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Citations for Millipore Sigma :
3151 - 3200 of 10000+ citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade E CM244 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... a known quantity of EV protein was mixed with 1% ultra-low gelling temperature agarose (Sigma Aldrich, Oakville, ON, Canada), 1% gelatin (Sigma Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... PTP-tagged or MHTAP-tagged protein variants were detected using the peroxidase-anti-peroxidase soluble complex (PAP) (1:2000, Sigma) as previously described.10 Detection of 6x His-tagged recombinant POLIB variants was performed with primary antibody Penta•His (1:1000 ...
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bioRxiv - Plant Biology 2022Quote: ... 1 mL of filtered protein extract was mixed with 40μL of anti-FLAG M2 magnetic beads (Merck, formerly Sigma-Aldrich) (equilibrated in GTEN + 0.1 % Tween-20 prior to use ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1 μg of FhNEJ-Teg or recombinant protein in the presence or absence of 15 ng t-PA (Sigma) or 10 ng u-PA (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... and the double phosphorylated form of S6 ribosomal protein (p-Ser240-244 -S6rp) were simultaneously detected through incubation with combined polyclonal goat anti-ChAT (1:500, #AB144P, Millipore) and polyclonal rabbit anti-p-Ser240-244 -S6rp (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated overnight with the following primary antibodies diluted in block: Anti-myelin basic protein (MBP, 1:100, Millipore #MAB386), anti-NeuN (1:500 ...
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bioRxiv - Microbiology 2024Quote: ... A total of 1 μg MBP-fusion protein (in 100 μl of 1% NP40) was incubated with 0.5 μg Avicel microcrystalline cellulose (Sigma-Aldrich) for 3 hours at 4°C with rocking ...
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bioRxiv - Neuroscience 2024Quote: ... The protein extracts (50 µg) were then in-gel digested with trypsin overnight (1:100, w/w, Sigma-Aldrich, USA). Peptides were applied to a reverse-phase liquid chromatographer [Acquity UPLC M-Class System (Waters Corporation ...
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bioRxiv - Molecular Biology 2024Quote: ... For shRNA-mediated knockdown, all TRC pLKO.1 vectors (targeting the YTH domain-containing proteins, THOC1, and THOC2) were purchased from Sigma. The TRIPZ doxycycline (Dox)-inducible lentiviral YTHDC1 shRNA vector is purchased from Dharmacon.
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated overnight at 4 °C with antibodies that recognise specific proteins expressed by each cell type such as mouse anti-NeuN (1:250; Millipore) to label neurons ...
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bioRxiv - Cell Biology 2024Quote: Doxycycline-inducible stable Flp-In T-REx HEK293 cell lines expressing mitochondrial-relocated golgin-BirA* fusion proteins were induced with 1 μg/ml doxycycline (Sigma) in culture medium once they reached approximately 80% confluency ...
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bioRxiv - Cell Biology 2024Quote: ... TRPM2-FLAG protein was probed with mouse anti-FLAG®M2 antibodies (F1804, Sigma; primary, 1:5000 in blocking buffer) in conjunction with HRP-conjugated goat anti-mouse IgG (A16078 ...
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bioRxiv - Biochemistry 2024Quote: ... The purified protein sample was concentrated to 40 mg mL-1 using Amicon Ultra-15 3K MWCO Centrifugal Filter Unit (Merck Millipore) and subsequently diluted 1:4 with MilliQ water (yielding 10 mg mL-1 of protein in 1x crystal buffer (10mM Tris ...
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bioRxiv - Cell Biology 2024Quote: ... The hexahistidine-tagged protein was expressed in Hi5 insect cells (Trichoplusia ni) grown in 1 liter of M3 medium (Sigma) supplemented with 10% fetal bovine serum (Wisent) ...
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bioRxiv - Biophysics 2024Quote: ... Buffer exchange to 1 x PBS was performed using desalting column (Generon) and eluted protein was concentrated with 30kDa MWCO Amicon (Millipore) to 20 mg/mL ...
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bioRxiv - Biochemistry 2024Quote: ... The final protein was concentrated to ∼10 mg ml-1 using a 30 kDa molecular weight cut-off concentrator (Amicon Ultra, Millipore). The concentrated protein was flash-frozen and stored at −80 °C until further use.
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bioRxiv - Plant Biology 2023Quote: ... 5-day- old light- and dark-grown seedling protein extract was incubated with 1 µl anti- phosphoserine antibody (Sigma, USA) with gentle rotation for 2h at 4°C followed by centrifugation and subjecting the pellet to western blotting as described by Matsushita et al ...
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bioRxiv - Molecular Biology 2023Quote: ... the FLAG-AGO1 proteins were detected with anti-FLAG-HRP antibody (1:4000 [Sigma] in 5 % milk in PBS-T). The membrane was developed as described above with homemade ECL or SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... the purified proteins and domains were pooled and concentrated to 0.2–1 ml in PBS buffer by Amicon Ultra-15 (Millipore, USA). A BCA Protein Assay Kit (Beytotime Bio Co Ltd. ...
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bioRxiv - Cell Biology 2023Quote: For LC-MS/MS, proteins were reduced and alkylated in SDC buffer (1% Sodium deoxycholate, 40 mM 2-Cloroacetamide (Sigma), 10 mM TCEP (Thermo ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were pre-cleared at 4°C for 1 h on a rotating wheel using 30 µl Protein-S agarose beads (Merck Millipore) slurry ...
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bioRxiv - Cell Biology 2023Quote: ... coli strains BL21 (D3E)/ pRARE carrying the corresponding expression vectors were induced for protein expression with 0.5 mM isopropyl-1-thio-D-galactopyranoside (IPTG, Sigma-Aldrich) for three hours at 30 ° C ...
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bioRxiv - Microbiology 2023Quote: ... Cleared cell lysates were immunoprecipitated using 1 μg FLAG-M2 antibody coupled to 40 μL of packed protein G Sepharose beads (Cat GE17-0618-01, Sigma) for 3 h at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Immunoprecipitation was achieved by incubating cell lysates containing 1 mg of protein overnight with anti-HA agarose beads (Sigma-Aldrich), Myc-agarose beads (Santa cruz biotechnology ...
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bioRxiv - Cell Biology 2023Quote: ... and overexpression of the proteins was induced by 0.1–0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG, Sigma Aldrich, #I5502) for 20 hrs at 18°C for His6-tagged caltubin or 3 hrs at 30°C for GST-tagged caltubin ...
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bioRxiv - Immunology 2023Quote: ... 50 µg protein in 1× Laemmli buffer was separated by SDS-PAGE and then transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore). The PVDF membranes were blotted with primary antibodies ...
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bioRxiv - Biochemistry 2023Quote: ... cell lysates were normalized by dilution to a common protein concentration at 1 mL total volume and pre-cleared with 4B Sepharose beads (Sigma) at 4°C for 2 h while rocking ...
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bioRxiv - Biochemistry 2023Quote: ... The column was washed with 10 column volumes of FLAG buffer and proteins were eluted as 1 ml fractions with 100 μg/ml FLAG peptide (Sigma). 5 mM DTT was added to each fraction and peak fractions as determined from Coomassie blue gel electrophoresis were pooled and concentrated in Vivaspin 15-max speed filter (MWCO 30kD ...
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bioRxiv - Biochemistry 2022Quote: ... FLAG-tagged L protein mutants were detected using horseradish peroxidase-conjugated anti-FLAG M2 antibody (1:9,000) (A8592; Sigma-Aldrich) and bands were visualised by chemiluminescence using Clarity Max Western ECL Substrate (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were processed with a free-floating section staining protocol for immunofluorescence using unconjugated primary antibodies (βIV-Tubulin, T7941, Sigma‒Aldrich, dilution 1:400; GFAP, glial fibrillary acidic protein, G-A-5, Sigma‒Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... anti-mNG (Vanderbilt Antibody and Protein Resource Core, VU527) (1:1000) or anti-ɑ-tubulin (Sigma-Aldrich Cat# T5168, RRID:AB_477579) (1:5000 ...
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bioRxiv - Biophysics 2023Quote: The LAF-1 protein coding sequence was ordered as a gBlock Gene Fragment (TM) and inserted into a pET28a(+) vector from Novagen. The LAF-1 mutants E398A (DAAD ...
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bioRxiv - Biochemistry 2023Quote: ... FLAG-tagged L protein mutants were detected using horseradish peroxidase-conjugated anti-FLAG M2 antibody (1:9,000) (A8592; Sigma-Aldrich) and bands were visualised by chemiluminescence using Clarity Max Western ECL Substrate (Bio-Rad ...
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Arabidopsis actin-binding protein WLIM2A links PAMP-triggered immunity and cytoskeletal organizationbioRxiv - Plant Biology 2023Quote: ... Proteins were reduced at 37 °C for 1 hour with 10 mm Tris(2-carboxyethyl)phosphine (TCEP, C-4706 Sigma) in 100 mm NH4HCO3 ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were separated on SDS-PAGE gels and analyzed by western blotting (anti-FLAG M2, 1:1,000 dilution; Sigma, F1804)
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Treg cells drive MYCN-mediated immunosuppression and tumor aggressiveness in high-risk neuroblastomabioRxiv - Cancer Biology 2023Quote: ... CAC-NIH-NA-02)42–44, or anti-zebrafish Ncam1 antibody (1:100, Institute for Protein Innovation) in PBS containing 10 U/mL Heparin (Sigma), 10% FBS ...
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bioRxiv - Cell Biology 2024Quote: ... Overexpression of the fusion protein was induced by adding 1 mM isopropyl-D-thiogalactopyranoside (IPTG; Sigma Aldrich; St. Louis, MO) to the transformed cell culture ...
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bioRxiv - Neuroscience 2024Quote: ... The samples were then blocked in 6% donkey serum and immunolabeled for protein gene product 9.5 (PGP9.5, 1:500, Millipore-Sigma, SAB4503057-100UG) as a pan-neuronal marker or rabbit-IgG (Abcam ...
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bioRxiv - Cell Biology 2024Quote: ... HA-tagged proteins were probed with a 1:1000 dilution of Roche rat anti-HA monoclonal 3F10 primary (Sigma 11867423001) and a 1:5000 dilution of donkey anti-rat DyLight800 (Invitrogen Life Technologies SA5-10032) ...
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bioRxiv - Cell Biology 2024Quote: ... and following centrifugation immunoprecipitations were done using supernatant (I mg protein) and anti-FLAG antibody (Sigma #F1804; dilution 1:1000) and Protein G agarose beads (60 µL ...
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bioRxiv - Molecular Biology 2024Quote: ... 500 µg of protein was diluted to a concentration of 1 µg/µl and 2 µg of anti-FLAG M2 antibody (Sigma) was added and incubated overnight at 4°C for IP ...
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bioRxiv - Cell Biology 2019Quote: ... or western blotting using anti-Protein A from rabbit (for detecting TAP fusion proteins; P3775; Sigma), anti-myc from mouse (9E10 ...
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bioRxiv - Cancer Biology 2021Quote: ... antibody overnight and then with equilibrated protein G beads (Millipore Sigma, Protein G Agarose, Fast Flow) for 2h ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein-protein interactions were assessed using the PLA Duolink in situ starter kit (Sigma Aldrich, DUO92101) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations were estimated by the bicinchoninic acid (BCA) protein assay method (Sigma-Aldrich, Merck, USA). Proteins (50 μg ...
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bioRxiv - Cancer Biology 2022Quote: ... Total protein concentration was determined using the Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich Cat# BCA1) as per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Protein-protein interactions were detected using the Detection Kit Red (DUO92008) according to the manufacturer’s (Sigma) instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... and 100 µg protein was incubated with 20 µl packed protein G-sepharose beads (Sigma P3296) at 4°C for 1 hr for preclearing ...
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bioRxiv - Biochemistry 2019Quote: ... 2 µg of Rab protein were mixed with 5x SYPRO® orange protein gel stain (Sigma) in a volume of 20 µl ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration of the collagen was verified by both BCA Protein assay (Sigma-Aldrich, cat: BCA1) and drying via vacuum centrifugation.