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Citations for Millipore Sigma :
3051 - 3100 of 10000+ citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade E CM244 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Clarified lysates were incubated with 1.5 µg polyclonal anti-V5 or IgG for 1 h and captured with protein A agarose resin (P2545, Sigma) for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Nanobody fusion proteins were detected using a primary anti-HA (mouse; 1:3,000, Sigma-Aldrich, St. Louis, MO, USA). An anti-mouse IgG-horseradish peroxidase (HRP ...
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bioRxiv - Microbiology 2022Quote: Cleaved protein was concentrated to ~1 mL using an Amicon Ultra-15 centrifugal filter 30 kDa MWCO (EMD Millipore), then loaded onto a HiLoad Superdex S200 pg gel filtration column (GE Healthcare Life Science) ...
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bioRxiv - Plant Biology 2024Quote: ... resuspended in 1 mL total protein extraction buffer (10 mM Phosphate Buffered Saline (PBS) at pH 7.4 (Sigma P5368), 0.5% v/v NP-40 (Nonidet™ P 40 Substitute from Sigma 74385) ...
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bioRxiv - Plant Biology 2024Quote: ... His- and GST-tagged proteins were detected with anti-His (Monoclonal Anti-polyHistidine antibody, Sigma H1029, 1:5000 dilution) or anti-GST (Mouse anti-GST monoclonal antibody ...
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bioRxiv - Microbiology 2024Quote: ... HA tagged proteins were detected in cell lysates using mouse monoclonal anti-HA antibody (Cat# H9658, Sigma, 1:10,000). HA tagged proteins were detected in virus lysates using rabbit polyclonal anti-HA antibody (Cat# H6908 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the equivalent of 500-800 µg of protein was incubated with 1 µg of anti-GFP antibody (Sigma-Aldrich) and then incubated with Protein A/G magnetic beads (Thermofisher ...
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bioRxiv - Microbiology 2024Quote: ... The purified protein was concentrated to ∼1 mg/mL by centrifugal filtration (AmiconTM 30K, Millipore Sigma, Burlington, MA, USA), flash frozen in liquid nitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... An equal amount of protein was used for immunoblot analysis using an anti-HA antibody (1:1000 dilution, Sigma) and anti-cMyc antibody (1:1000 dilution ...
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bioRxiv - Biochemistry 2023Quote: ... 10 μL of this dilution was then added to 90 μL of buffered D2O (prepared 5 mL with 4.5 mL of D2O and 0.5 mL of 10x protein dilution buffer and 0.2 μg/mL of peptide standard Glu-1-Fibrino peptide (CAS: 103213-49-6, Sigma)) to initiate deuteration at 22 °C ...
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bioRxiv - Microbiology 2023Quote: ... THP-1 cells (100% confluent) were harvested and lysates prepared using CytoBuster protein extraction reagent (Novagen/EMD, Gibbstown, NJ) supplemented with complete mini EDTA-free protease inhibitor (Roche ...
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bioRxiv - Immunology 2023Quote: Male mice were sensitized with 4 mg of PN butter (∼1 mg of protein; Capitán Mani) and 5 μg of cholera toxin (C8052, Sigma) in 0.5 mL of PBS administered intragastrically ...
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bioRxiv - Cancer Biology 2023Quote: ... 210 ug of protein was digested using Lys C (Wako) at an enzyme:protein ratio of 1:100 and trypsin (Sigma) at 1:50 ratio overnight at 37 °C ...
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bioRxiv - Biochemistry 2023Quote: ... and the protein samples were concentrated to 1-2.5 mg/mL using a concentrator (cut-off: 100 kDa, Amicon Ultra, Millipore). Representative SEC profiles and Coomassie blue stained SDS-PAGE gels for the Closed ...
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bioRxiv - Biochemistry 2023Quote: ... followed by resuspension of the dry pellets by vortexing (≈1 min) and subsequent addition of 150 μL lysis buffer (HEPES buffer containing 0.35X BugBuster Protein Extraction Reagent (Novagen) and 0.1% Lysonase Bioprocessing Reagent (Novagen)) ...
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bioRxiv - Biophysics 2023Quote: ... a solution of 50 μM αS and 1 μM N-protein in 20 mM Tris buffer (Sigma-Aldrich, UK), pH = 7.4 ...
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bioRxiv - Biochemistry 2022Quote: ... HMCES-3flag protein was detected by immunoblotting with anti-flag antibody (1:10,000 dilution in PBST; Sigma, F1804-200UG) and rat anti-mouse secondary antibody peroxidase conjugate (1:20,000 dilution in PBST ...
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bioRxiv - Molecular Biology 2023Quote: ... and the eluted proteins were subjected to Western Blot analysis using a GST antibody (1:1000, Millipore 05-782).
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bioRxiv - Molecular Biology 2023Quote: The following commercially available antibodies were used to detect targeted proteins: anti-RBM17 (1:1500 dilution; HPA037478, Sigma-Aldrich), anti-SF3B1 (1:3000 dilution ...
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bioRxiv - Cancer Biology 2024Quote: ... Detection of specific protein bands was accomplished using a rabbit monoclonal anti-GLRX3 antibody (1:1,000, HPA028941, Sigma-Aldrich), ferritin (1:1,000 ...
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bioRxiv - Microbiology 2024Quote: ... Eluted proteins were concentrated to 2.5 mg mL-1 using 100 kDa molecular weight cut-off centrifugal filters (Merck Millipore). To each 200-mesh holey carbon grid (Au R1.2/1.3 ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were detected after incubating with peroxidase conjugated secondary antibodies for 1 hour and 5min with ImmobilonR Western (Millipore) after 5x washes ...
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bioRxiv - Immunology 2020Quote: ... TZM-bl cells infected with JR-FL without Env (No Env virus) were treated with 100 mM 2-deoxy-glucose (2-DG) (Sigma-Aldrich, St. Louis, MO, USA) 2 h prior to image acquisition.
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bioRxiv - Microbiology 2024Quote: ... using 100 mg of human stool sample per 1 ml of PBS supplemented with 0.1% L-cysteine and 15% glycerol (Sigma-Aldrich, St-Louis, MO, USA). The suspension was incubated for 10 min to allow large particles to settle ...
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bioRxiv - Bioengineering 2024Quote: ... dissociated hiPSC-Cardiomyocytes and primary cardiac fibroblasts were mixed in a 3:1 ratio and resuspended in fibrinogen by mixing the cell solution with 33 mg/mL human fibrinogen (Sigma-Aldrich, cat. no. F3879) and RPMI-B27 (described above ...
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bioRxiv - Cell Biology 2023Quote: The following primary and secondary antibodies were used at indicated concentrations: anti-human α-actinin1 (mouse monoclonal, BM75.2, Sigma Aldrich, A5044; WB 1:1000), anti-human talin (mouse monoclonal ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 3 units/ml of human EPO (Peprotec, #100-64) and 10 ng/ml of G-CSF (Filgrastim, Millipore Sigma, 121181-53-1) (Stem Cell Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies for DNA damage assays were diluted in PBS with 10% SuperBlock at the following dilutions: anti-human γH2AX Ser139 (EMD Millipore; JBW301; 1:200), goat anti-mouse IgG AF546 (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... Even protein loading and successful protein transfer was confirmed using Ponceau S stain (Sigma Aldrich) prior to probing with primary antibodies (Table S2) ...
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bioRxiv - Cell Biology 2022Quote: ... for total protein loading quantification before proteins were transferred to PVDF membrane (Millipore, Cat. # IPVH00010). The blots were incubated with primary antibodies against SUMO2/3 (Enzo Life Sciences ...
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bioRxiv - Immunology 2021Quote: ... with protein concentrations normalized to total protein levels in lysate (BCA assay; Sigma-Aldrich®).
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bioRxiv - Microbiology 2019Quote: ... and the protein level was measured with the Enhanced BCA Protein Assay kit (Sigma-Aldrich). The extracts which contained 25 μg of total proteins were subjected to 10% SDS polyacrylamide gel ...
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bioRxiv - Cell Biology 2021Quote: ... and the protein concentration was estimated using the Bio-Rad Protein Assay Dye Reagent (Sigma). 10 µl of liver lysate was transferred to 96-well plate and ALT was measured using a microplate-based ALT activity assay kit (Pointe Scientific ...
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bioRxiv - Cell Biology 2020Quote: Proteins of cellular membrane were extracted using the ProteoExtract® Transmembrane Protein Extraction Kit (NOVAGEN). Total 500 μg of membrane proteins were used for immunoprecipitation with an anti AT1-R or LOX-1 antibody ...
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bioRxiv - Cell Biology 2019Quote: ... Protein concentration was determined using a BCA protein concentration assay kit (Sigma, Shanghai, China, FP0010). Equal amounts of protein (20 μg ...
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bioRxiv - Cancer Biology 2020Quote: ... the concentration of EV protein was quantified by using a BCA Protein Assay Kit (Novagen). For BMDC stimulation by EVs ...
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bioRxiv - Cancer Biology 2021Quote: ... the protein-protein interaction was identified using a commercially available PLA kit (Duolink; Sigma-Aldrich) as described before 34 ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein complexes were pulled down by 1.5 h incubation with Protein A agarose beads (Millipore) or GFP-Trap®_A beads (Chromotek) ...
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bioRxiv - Cell Biology 2020Quote: Proteins from 3T3-L1 fibroblast lysates extracted with Cytobuster protein extraction reagent (Novagen®, Merck). After cell disruption for 10 min at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were separated by SDS-PAGE and biotinylated proteins detected using Streptavidin-HRP (Sigma # RABHRP3) and Supersignal detection kit (Thermofisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... The fusion protein was treated with SUMO protease (Sigma SAE0067, 5 U/ mg target protein) to liberate Mpro with authentic N-(Ser1 ...
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“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”bioRxiv - Developmental Biology 2024Quote: ... Total proteins extraction from hPSCs was performed in ice-cold RIPA protein extraction buffer (Sigma) supplemented with protease inhibitors (Protease inhibitor cocktail set I ...
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bioRxiv - Plant Biology 2023Quote: ... Protein levels were then quantified using a Coomassie protein assay kit (B8522, Sigma-Aldrich, USA) followed by 12% SDS-PAGE separation (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Protein concentrations were then normalized to 6 μg protein and the KGDH assay kit (Sigma) protocol was followed.
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bioRxiv - Cancer Biology 2023Quote: ... The protein-protein interaction was investigated using a commercially available PLA kit (Duolink; Sigma-Aldrich) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentrations were determined by Bicinchoninic Acid Kit for Protein Determination (BCA1-1KT, Sigma Aldrich) or the Bradford Protein Assay (BioRad) ...
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bioRxiv - Genetics 2023Quote: ... Protein expression was verified and quantified by Western blot using recombinant GST protein (Sigma, G5663) as expression standards ...
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bioRxiv - Cancer Biology 2023Quote: ... and protein concentration in the supernatant was measured using the BCA Protein Assay kit (Sigma).
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bioRxiv - Cancer Biology 2024Quote: ... Samples were then washed with buffer and recombinant Tau protein (Sigma recombinant Tau protein #T0576) and ATP (Sigma #A1852 ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysates were pre-cleared by Protein G Plus/Protein A Agarose Suspension (Merck Millipore #IP05). The lysates were then separately incubated with truncated form antibodies (i ...