Labshake search
Citations for Millipore Sigma :
3001 - 3050 of 10000+ citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade E CM244 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2023Quote: ... 10ug human epithelial growth factor (hEGF) and 10% FBS (Sigma); LNCaP ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were purified on collagen IV from human placenta (Sigma) and human plasma fibronectin (Life Technologies ...
-
bioRxiv - Biophysics 2023Quote: The MG63 human osteosarcoma cell line (Sigma, St. Louis, USA) was obtained from the Health Security Agency ...
-
bioRxiv - Microbiology 2023Quote: - fibronectin (Human plasma fibronectin, Sigma-Aldrich, USA, cat. no. FC010) 1 mg/mL solution in autoclaved distilled water,
-
bioRxiv - Cell Biology 2023Quote: ... 3% v/v heat-inactivated Human Male AB Serum (Sigma), 2 mg/ml Human Serum Albumin (HSA ...
-
bioRxiv - Developmental Biology 2023Quote: ... and 5 IU of hCG (Human chorionic gonadotrophin, Sigma-Aldrich) with a 48-hour interval between injections ...
-
bioRxiv - Cell Biology 2024Quote: ... and 0.2 U/ml human insulin (Sigma-Aldrich, I9278-5ML). MCF7 cells were cultured in DMEM (VWR ...
-
bioRxiv - Cell Biology 2024Quote: ... 10 μg/mL human apo-Transferrin (Sigma-Aldrich; cat. #T1147), 2.6 ng/mL sodium selenite (Sigma-Aldrich ...
-
bioRxiv - Bioengineering 2024Quote: Human fibrinogen from non-diseased donors (Merck Millipore, #341576-1GM) was reconstituted in sterile 0.9% saline to a stock concentration of 67mg/ml ...
-
bioRxiv - Bioengineering 2024Quote: Human dermal fibroblasts were available in-house (Sigma, #106-05a). Depending on experiments ...
-
bioRxiv - Bioengineering 2024Quote: ... was coated with 10ug/ml human fibronectin (EMD Millipore, FC010) for 30 min at 37°C then washed three times with deionized water (DI) ...
-
bioRxiv - Biophysics 2024Quote: ... using either recombinant human EGF (Sigma-Aldrich, St. Louis, MO) or Ephrin A1 as ligand ...
-
bioRxiv - Biophysics 2024Quote: ... using either recombinant human EGF (Sigma-Aldrich, St. Louis, MO) or NRG1 as ligand ...
-
bioRxiv - Immunology 2024Quote: ... and human monoclonal IgE (2 µg/ml; Sigma-Aldrich, #401152) to enhance the expression of the FcεRI before stimulation (Saleh et al. ...
-
bioRxiv - Neuroscience 2024Quote: ... and 5 mg/mL human insulin (cat#: I9278; Millipore Sigma), freshly supplemented with 100 ng/mL IL-34 (cat# ...
-
bioRxiv - Molecular Biology 2024Quote: ... human lymphoblast cells were maintained in RPMI-1640 media (Sigma), and U2OS (ATCC 30-2007 ...
-
bioRxiv - Cell Biology 2024Quote: ... and 0.023 U/ml human insulin (Sigma-Aldrich, I9278-5ML). HCC1937 cells were cultured in RPMI-1640 medium (VWR ...
-
bioRxiv - Cell Biology 2024Quote: ... and 0.01 mg/ml human insulin (Sigma-Aldrich, I9278-5ML). U2OS cells were purchased from Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures and cultured in Dulbecco’s Modified Eagle’s Medium (DMEM ...
-
bioRxiv - Systems Biology 2024Quote: Ovulation was induced by injection of human chorionic gonadotropin (Sigma) into the dorsal lymph sac according to standard procedure166 and in accordance with approved UCSF IACUC protocols ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 10 mU/mL human insulin (Sigma-Aldrich, I9278-5ML). The fGS/OSS cells were cultured using 25% conditioned medium (from the old culture ...
-
bioRxiv - Cell Biology 2020Quote: ... Lamin A proteins were detected with mouse anti-myc antibodies (clone 4A6, Millipore cat #05-724; 1:10,000 dilution) decorated with goat anti-mouse secondary IRDye 680RD antibodies (LI-COR ...
-
bioRxiv - Microbiology 2021Quote: ... Sized proteins were brought to concentrations typically >50 mg mL−1 using appropriate molecular weight cut-off concentrators (Millipore), flash frozen on liquid nitrogen ...
-
bioRxiv - Molecular Biology 2020Quote: ... The appropriate horseradish peroxidase-coupled secondary antibodies were used to reveal the proteins (antimouse at 1:10,000 (Sigma, A2554) and antirabbit at 1 ...
-
bioRxiv - Cell Biology 2022Quote: ... were added for 1 hour at room temperature and protein signal was then visualized using Immobilon Forte (Millipore, WBLUF0500) on ImageQuant LAS 4000 or Amersham ImageQuant 800 ...
-
Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: ... Then slides were incubated with antibodies specific to acrosin binding protein (ACRPB) (1:100 in PBS) (Sigma; HPA039081, USA), IZUMO1 (1:100 in PBS ...
-
bioRxiv - Neuroscience 2019Quote: ... proteins were transferred electrophoretically to polyvinylidene difluoride membranes and probed with the following antibodies:anti-EphB6 (1:500, SAB4503476, Sigma), anti-EphB1 (1:500 ...
-
bioRxiv - Molecular Biology 2019Quote: ... Bound proteins were cleaved off the column with thrombin diluted 1:250 in thrombin cleavage buffer (Novagen #69671-3). The thrombin solution was loaded manually into the column with a syringe ...
-
bioRxiv - Cell Biology 2019Quote: ... the proteins were blotted onto a nitrocellulose membrane and probed with anti-GFP (1:1000; Sigma-Aldrich and Roche), anti-dgt6 (1:500 ...
-
bioRxiv - Biochemistry 2019Quote: ... Proteins were resuspended in a solution containing 1 % SDC and 10 mM TCEP with 40 mM CAA (Sigma-Aldrich) as reduction and alkylating agents and heated to 37 °C for 1 hour ...
-
bioRxiv - Cell Biology 2021Quote: Expression of Flag- and EGFP-tagged proteins was induced with media containing 1 µg/mL doxycycline (Sigma-Aldrich D9891) 12-24 hours prior to harvesting ...
-
bioRxiv - Biochemistry 2020Quote: ... The protein was concentrated to 40 mg ml-1 with a centrifugal filter device (Millipore 50 kDa MW cutoff), and frozen until crystallization.
-
bioRxiv - Neuroscience 2021Quote: ... the cells were incubated with the primary antibodies of interested proteins at 4°C overnight (Nestin, 1:1000, Sigma; Tuj1 ...
-
bioRxiv - Biochemistry 2020Quote: ... Protein post cleavage was injected into FPLC and run over two HisTrap FF Crude 1 mL columns (Sigma-Aldrich) with FPLC running buffer ...
-
bioRxiv - Biochemistry 2020Quote: ... NaCl was removed from the buffer and the protein was concentrated to 100 mg mL−1 using a 50,000 Da molecular mass cut-off concentrator (Millipore). The concentrated protein was incubated with a four-fold molar excess of dalbavancin at 4 °C for 12 h ...
-
bioRxiv - Plant Biology 2020Quote: ... The protein extraction buffer (50 mM TrisHCl, pH 7.0, 1% w/v SDS, P9599 protease inhibitor cocktail, Sigma-Aldrich) was added to the powder ...
-
bioRxiv - Systems Biology 2021Quote: ... separated proteins were transferred to a PVDF membrane using a semi-dry system and His-tagged proteins (hCDKL5 and His-Neuropilin) were detected with a HRP-conjugated anti-His antibody (1:2,000, Sigma) using the Enhanced Chemiluminescence kit (ECL ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cells were blocked with 3% w/v BSA and incubated with 1:16,000 v/v rabbit anti-protein A antibody (SIGMA) for 1 h at room temperature ...
-
bioRxiv - Cell Biology 2022Quote: ... The glass surfaces were coated with 1 mg/mL of BSA protein (Albumin, biotin-labeled bovine, A8549 Sigma-Aldrich) by incubating for 2 hours ...
-
bioRxiv - Neuroscience 2022Quote: Mouse monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) antibody (diluted 1:200; cat. no. G3893; Sigma-Aldrich, MO, USA) and Goat anti-Mouse IgG (H+L ...
-
bioRxiv - Physiology 2019Quote: ... FLAG-tagged protein were eluted twice with 200 ng/μL FLAG peptide (Sigma, #F3290; 1 h at 4°C). PL transfer activity was assayed using NBD labeled Phosphoethanolamine ...
-
bioRxiv - Biochemistry 2019Quote: ... and proteins were resuspended in 100 μL of 100 mM ammonium acetate and 1 μg trypsin (Proteomics grade, Sigma). Proteins were digested by incubation at 37 °C for 16 h.
-
bioRxiv - Developmental Biology 2019Quote: ... proteins were transferred to a PVDF membrane and immunoblotted with an anti-phosphotyrosine antibody (1:1000 dilution, Upstate/Millipore) for chemiluminescence detection.
-
bioRxiv - Genetics 2021Quote: ... Reversal of TetR fusion protein binding was achieved by addition of 1 μg/ml Doxycycline (final concentration / Sigma-D9891) to mESC culture medium.
-
bioRxiv - Biochemistry 2021Quote: ... FLAG-tagged L protein mutants were detected using peroxidase-conjugated anti-FLAG M2 antibody (1:10,000) (A8592; Sigma-Aldrich) and bands were visualized by chemiluminescence using Super Signal West Femto substrate (Thermo Scientific ...
-
bioRxiv - Immunology 2021Quote: ... Efficiency of the expression and purification of recombinant proteins were determined by 15% SDS-PAGE and Western blotting by using anti-his tag antibodies (1:10,000) (Sigma). Protein concentrations were quantified by comparison with a curve of BSA resolved by SDS-PAGE.
-
bioRxiv - Immunology 2021Quote: ... 1 mM EDTA) buffer and OAS1 proteins were immunoprecipitated from the lysate using 20 µg anti-FLAG antibody (Sigma) and Dynabeads Protein G ...
-
bioRxiv - Genetics 2020Quote: ... Proteins were detected using either secondary anti-mouse or anti-rabbit IgG coupled to peroxidase (Millipore, 1:5000 dilution), western bright ECL substrate (Advansta ...
-
bioRxiv - Biochemistry 2022Quote: ... 4 mM DTT and 5% D2O at a protein concentration 1 mM using a Shigemi NMR tube (Sigma Aldrich). Data was collected on an ultrahigh field Bruker 900 MHz NMR spectrometer equipped with a triple resonance cryogenic probe ...
-
bioRxiv - Plant Biology 2022Quote: ... immunoblotting was performed with 50 μg of protein extracts using anti-Flag-M2-HRP (Sigma-Aldrich, A8592; 1:10,000), anti-c-myc-peoxidase (Roche ...
-
bioRxiv - Physiology 2022Quote: ... 80 μg of protein were incubated in 10 mM L-cysteine and 1 mM pyridoxal 5′-phosphate hydrate (Sigma); or ...