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Citations for Millipore Sigma :
3101 - 3150 of 10000+ citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade E CM244 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... The protein concentration was then determined using a Pierce BCA protein assay (Sigma-Aldrich; 71285) and intact cell respiration was normalized to milligrams of total intact cell protein.
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bioRxiv - Cancer Biology 2024Quote: ... The protein concentration was then determined using a Pierce BCA protein assay (Sigma-Aldrich; 71285).
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bioRxiv - Cell Biology 2019Quote: ... The desired Young’s modulus (E) of the pre-mixes was adjusted by mixing pre-defined ratios of 40% (w/v) acrylamide monomer (Sigma-Aldrich, A4058) and 2% (w/v ...
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bioRxiv - Developmental Biology 2021Quote: ... cells from the ATOH1-EGFP line were gently scraped off Transwell membranes and plated on poly-D-lysine (Millipore, A-003-E) and Laminin (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... HEK293T and U-2 OS cells were seeded on to poly-D-lysine (Cat. No. A-003-E, Sigma, St. Louis, USA) pre-coated 24-well plates at 24 h prior to transfection.
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bioRxiv - Genomics 2019Quote: ... 2×105 HEK293T cells were plated onto each well of a 25 ug/ml poly-D lysine (EMD Millipore, A-003-E) coated 24-well plate ...
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bioRxiv - Synthetic Biology 2021Quote: ... rhaeticus cultures were grown shaking to mid exponential phase. Cells treated with 50 nM (E. coli) or 50 μM (K. rhaeticus) of AHL (N-Acyl homoserine lactone, Sigma-Aldridge K3255) and induced for approximately 4 doubling times (i.e ...
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bioRxiv - Microbiology 2021Quote: ... Pooled culture media containing E/S was concentrated by centrifugation using Amicon ultra centrifugal filter units (MWCO 10 kDa, Sigma-Aldrich, Denmark), and filtered prior to testing of protein content by bicinchoninic (BCA ...
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bioRxiv - Microbiology 2022Quote: ... 5°6.7’ E) in September and October 2017 by filtering 2 L of seawater through PVDF membrane filters (Merck Millipore, Burlington, Massachusetts,USA), pore size ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were washed 2X with YPRaff media (1X with YPRaff and 1X with YPRaff + 50 μg/ml Pronase E (Sigma-Aldrich, 1074330001)) ...
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bioRxiv - Developmental Biology 2023Quote: H&E and tartrate-resistant acid phosphatase (TRAP) staining were performed according to the manufacturer’s protocols (Sigma, St. Louis, MO, United States) as described earlier [25] ...
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bioRxiv - Molecular Biology 2023Quote: ... MV4-11 cells were attached overnight by coating the coverslips in poly-D-lysine (A003-E; Sigma Aldrich; 100µg/ml in H2O) for 1 h ...
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bioRxiv - Genetics 2023Quote: ... The blocks were cut into 4 μm sections and stained with hematoxylin and eosin (H&E) to visualize morphology and with potassium hexacyanoferrate(II) trihydrate (Sigma cat# P3289) to visualize ferric iron deposits and counterstained with Neutral Red to visualize cell morphology (Sigma-Aldrich cat#72210).
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... Cellular monolayers were then incubated at 37 °C and under 5% CO2 in cell culture medium containing the lysosomal protease inhibitors E-64d (10 μg/mL final concentration; #E8640, Sigma-Aldrich, USA) and pepstatin A (10 μg/mL final concentration ...
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bioRxiv - Microbiology 2024Quote: Supernatants containing VSV(GFP)ΔG particles with S and E were layered on a cushion of 20% sucrose (Sigma-Aldrich, #S9378-1KG) in PBS and centrifuged for 90 min at 4°C and 20,817 x g ...
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bioRxiv - Immunology 2022Quote: ... The pseudotyped virus-mAb mixture were then overlayed onto target cell lines in the presence of 5 μg/ml polybrene (Sigma Aldrich, catalog no. TR-1003-G) and analyzed 48 h post-infection ...
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bioRxiv - Genomics 2020Quote: ... RRID:AB_2798177) overnight at 4°C or 1:10,000 dilution of monoclonal mouse anti-human β-actin (clone AC-74, Sigma-Aldrich, Cat. No. A2228, RRID:AB_476997) for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... MSCs embedded in the PEG-NB gels were stained for α-SMA with FITC-conjugated mouse monoclonal anti-human α-SMA (F3777, Sigma; dilution 1:300), cellular extra domain-A (ED-A ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated at 4 °C overnight in the same solution with the primary rabbit anti-OMP (olfactory marker protein; 1:500, O7889 from Sigma). Immunofluorescence was achieved by reaction with the secondary antibody donkey anti-rabbit-488 (A21206 from Molecular Probes ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were stained overnight at 4°C with primary antibody (Mouse anti-γH2AX antibody; Millipore Sigma #05-636, 1:300) diluted in blocking solution ...
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bioRxiv - Cell Biology 2022Quote: ... Immunohistochemistry was used to detect protein expression using the following antibodies on paraffin sections: SftpC (rabbit, Millipore, AB37086, 1:250), and Ki67 (eBioscience ...
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bioRxiv - Genomics 2019Quote: ... 0.04% (v/v) Tween-20) and FLAG-tagged protein were incubated for 1 hr with Anti-FLAG M2 Affinity Gel (Sigma) while rotating ...
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bioRxiv - Molecular Biology 2019Quote: ... 200 µg protein were incubated with DNase I (7,5 U) and 1 µg anti-Flag resin (cat# F3165, Sigma-Aldrich) at 12 rpm overnight at 4°C ...
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bioRxiv - Physiology 2019Quote: ... Hydrolyzed protein diet was prepared by incubating 5 mL intact protein diet with 43.7 mg trypsin (Trypsin Powder, Porcine 1:250, 85450C, Sigma Aldrich) at 37°C for 20 hours ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein digestion was performed overnight at 37 °Cwith Lys-C (Wako) at an enzyme/protein ratio 1:75 and trypsin (Sigma) at an enzyme/protein ration of 1:50 ...
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bioRxiv - Microbiology 2020Quote: ... and equal amounts of protein were analyzed using the following antibodies: anti-FLAG M2 (Sigma, cat#F3165, RRID:AB_259529; 1:7,500), anti-IE1 (clone 1B12 (Zhu et al. ...
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bioRxiv - Biochemistry 2021Quote: ... Protein extracts were incubated overnight with 1 μg of anti-PDGFRβ (D-6) antibody and immunocomplexes recovered using 25 µL Protein G Sepharose beads (Sigma) for 1 h 30 min in the wheel at 4°C ...
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bioRxiv - Biochemistry 2020Quote: A protein/Sypro orange mix containing 5 μM protein and a 1:5,000 dilution of dye in DMSO (as supplied by Sigma Aldrich) was prepared just before plate setup in 20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... Coverslips were labeled with antibodies as follows: HA-tagged proteins were detected with rat anti-HA 3F10 (Sigma 1:200), parasite cyst wall and parasite cytoplasm by in-house mouse SalmonE anti-CST1 (1:500 ...
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bioRxiv - Microbiology 2020Quote: ... Residual methylation reagents were neutralised by addition of 1M Tris-HCl pH 7.5 to a final concentration of 50 mM Tris-HCl and the protein complex was concentrated to 2 mg mL−1 using 30 kDa cut-off concentrators (Millipore) before injection into a S75 16/600 column (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... After incubation for 90 min at RT the microplates were washed 4X (PBS-Tween 20) and 25 μl of protein LA-HRP diluted 1:3000 (Sigma) was added and the microplates were then incubated for 1h at RT ...
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bioRxiv - Plant Biology 2021Quote: ... Wild-type and mutated Pik-1 and Pik-2 proteins were detected probing the membrane with anti-FLAG M2 antibody (Sigma) and anti-HA high affinity antibody 3F10 (Roche) ...
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bioRxiv - Cell Biology 2020Quote: ... the codon optimized gene encoding for rPMDs was subcloned into PET28a (+) and protein expression in E.coli cells was induced with 1 mM isopropyl-β-D-thiogalactoside (IPTG) (Sigma). The his-tagged proteins were purified using Ni-NTA chromatography and the concentration was determined using the BCA estimation kit (Pierce).
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bioRxiv - Biophysics 2020Quote: Wild-type or Y473E mutant human PPARγ LBD (UniProt P37231; residues 203–477, isoform 1 numbering) protein was expressed from a pET46 Ek/LIC vector (Novagen) as a Tobacco Etch Virus (TEV)-cleavable N-terminal Hexa(6x)His-tagged fusion protein in Escherichia coli BL21(DE3 ...
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bioRxiv - Cell Biology 2021Quote: ... and the dried protein pellet resuspended in digestion buffer (100 mM Tris-HCl pH 8.5, 1% sodium deoxycholate (Sigma-Aldrich), 5 mM TCEP ...
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bioRxiv - Biochemistry 2022Quote: ... The beads were washed three times with 0.5 ml of FLAG wash buffer and the bound protein was eluted with 1X SDS-PAGE loading buffer supplemented with 100 µg·ml-1 3X FLAG peptide (Sigma-Aldrich) on ice ...
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bioRxiv - Biochemistry 2022Quote: ... PKG Iβ (71-686) protein was concentrated to 20 mg ml-1 by using 30 kDa cutoff Amicon Ultra (Millipore) and initial crystal screening was performed by Mosquito Crystal robot (TTP Labtech ...
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bioRxiv - Microbiology 2022Quote: ... The cells were sonicated on a Sonics Vibracell VCX750 Ultrasonic Cell Disrupter (Labotal) and BugBuster protein extraction reagent (1:100 ratio) (Novagen) were added to complete the lysis ...
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bioRxiv - Biophysics 2022Quote: ... Protein was concentrated to 30-40 mg ml-1 using a 50-kDa cutoff Amicon Ultra centrifugal filter unit (Millipore). NpSxph crystals were obtained by hanging drop vapor diffusion at 4°C using 1:1 v/v ratio of protein and precipitant ...
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bioRxiv - Developmental Biology 2021Quote: ... 30 μl of Protein G coated Bio-Adembeads (Ademtech) were incubated with either 1 μg of mouse monoclonal anti-FLAG antibody (F3165, Sigma), or goat anti-HA antibody as mock (sc-805 ...
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bioRxiv - Molecular Biology 2020Quote: ... then 1-2 mg (as indicated) of total protein was incubated with pre-washed M2 α-FLAG magnetic beads (Sigma) or MagStrep “type3” XT beads (IBA ...
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bioRxiv - Plant Biology 2021Quote: ... and 1 × protease inhibitor cocktail] and precleared by incubation with 50 μL Protein-A agarose beads/Salmon sperm DNA (Millipore) at 4 °C for 1 h ...
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bioRxiv - Plant Biology 2021Quote: ... and the detection of the total MAPK3 and 6 protein levels were by using polyclonal anti-MAPK3 (1:1000, Sigma) and anti-MAPK6 (1:1000 ...
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bioRxiv - Cell Biology 2019Quote: ... antibodies were added for 1 hour at room temperature and protein signal was then visualized using Immobilon Forte (Millipore, WBLUF0500) on ImageQuant LAS 4000 (GE Healthcare Life Sciences).
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bioRxiv - Molecular Biology 2020Quote: ... 1.2mg Dynabeads-Protein-A was blocked overnight at 4°C in 1X IP buffer supplemented with 0.5mg ml-1 BSA (Sigma A7906). The antibody-RNA mixture was split into 50µl aliquots on ice and crosslinked with 0.15J cm-2 254nm UV radiation six times ...
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bioRxiv - Biochemistry 2020Quote: The gene encoding for the full-length protein of the CNFY C866S variant was cloned into a modified pCOLA Duet-1 vector (Novagen) encoding for an N-terminal Strep-tag II and TEV-protease recognition site (construct ...
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bioRxiv - Cancer Biology 2019Quote: Control pLKO.1-LacZ shRNA and four different lentiviral shRNA pLKO.1 plasmids designed to silence DARPP-32 protein expression were purchased from Sigma. To prepare the lentivirus ...
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bioRxiv - Genomics 2021Quote: ... 5 mM sodium metabisulfite and 1 mM MgCl2) containing 100 μg of purified protein and 100 μM GGPP (Sigma-Aldrich). The reaction mixture was overlaid with 500 μL of pentane (Macklin ...
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bioRxiv - Neuroscience 2020Quote: ... The purified protein was then left at room temperature overnight to facilitate protein folding and chromophore maturation before concentrating and buffer exchanging into 1× TBS using 10 kDa centrifugal filter units (Millipore). Unless noted otherwise ...
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bioRxiv - Neuroscience 2022Quote: ... Protein were transferred to polyvinylidene fluoride membranes and immunoblotted with monoclonal antibodies at ratio of 1:1000 (anti-N1, Millipore, MAB1586 ...