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Citations for Millipore Sigma :
2751 - 2800 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2020Quote: ... Membranes were washed three times as above and then developed with BCIP/NBT liquid substrate system (Sigma-Aldrich). The protein concentration of the FIV was determined using a BCA assay (Pierce #23227 ...
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bioRxiv - Neuroscience 2020Quote: ... Supernatants were collected and concentrated ten times in an Amicon 10 k centrifuge filter (Millipore Merck, Darmstadt, Germany) following the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... the plate was washed four times and incubated with HRP conjugated anti-horse whole IgG secondary antibody (Sigma) for 1 hr at RT ...
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bioRxiv - Neuroscience 2019Quote: ... were washed two times with PBS + 0.02% Tween and coated with anti-FLAG M2 monoclonal antibody (#F3165 Sigma). Thoraces or heads of adult flies were cut and stored in lysis buffer containing 20 mM Hepes ...
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bioRxiv - Physiology 2020Quote: ... The remaining precipitate was washed with cold acetone for three times (All reagents from Sigma-Aldrich and Calbiochem). The protein was redissolved in 8M urea and the protein concentration was determined with BCA Protein Assay Kit (P0009 ...
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bioRxiv - Cell Biology 2021Quote: ... for 10 min followed by washing three times with PBS and permeabilized with 0.2% Triton X-100 (Sigma) for 10 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Membranes were then washed three times with TBS-T and incubated with alkaline phosphatase-conjugated secondary antibodies (Sigma) in 5% milk in TBS-T for 30 min at room temperature ...
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bioRxiv - Pathology 2021Quote: ... The membrane was washed three more times in TBST before addition of Immobilon Western Chemiluminescence HRP Substrate (Millipore) and visualization via film development ...
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bioRxiv - Developmental Biology 2021Quote: ... the semen was diluted 104–105 times in ASW with 1 mM theophylline (Sigma-Aldrich Japan, Tokyo, Japan) to induce the activation of motility (7) ...
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bioRxiv - Immunology 2020Quote: ... Wells were washed three times with 1xDPBS and then blocked with1xDPBS containing 1% bovine serum albumin (Sigma-Aldrich) for one hour at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... the microtitre wells were washed three times with Tris Buffered Saline-Tween (TBST, pH 7.2-7.4) containing 0.05% v/v Tween 20 (Sigma-Aldrich) and 5mM CaCl2 (Thermo-Scientific), ...
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bioRxiv - Molecular Biology 2021Quote: ... washed 3 times with PBT-X (PBT with 0,3%Triton X100) and permeabilized in PBTX with 0,3% Sodium deoxycholate (Sigma) for one hour ...
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bioRxiv - Immunology 2020Quote: ... The cells were washed three times with 1X PBS and detached from plates using Accutase (A6964; Sigma-Aldrich) incubation for 15 minutes at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Compounds were identified by comparing sample retention times with 16 available authentic standards (Figure S1) (Sigma – Aldrich, EUA). The concentrations of the identified compounds have not been determined.
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bioRxiv - Cell Biology 2022Quote: ... At the end of the time course all plates were permeabilized with 0.5% Triton X-100 (Sigma-Aldrich) for 1 h and blocked with 3% Donkey Serum (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... washed three times with PBS and visualized by incubating the wells in 0.2% (w/v) crystal violet (Sigma)/10% ethanol for 15 min at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... The blot was then washed 3 times for 5 minutes each with pH 7.4 Tris-Buffered Saline (Quality Biological) with 0.1% Tween 20 (Sigma) (TBST ...
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bioRxiv - Biochemistry 2022Quote: ... Aliquots were taken at different time points and kept on ice with 3 units of nuclease-P1 (Sigma). One hour after nuclease-P1 incubation ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed three times and incubated in 4,6-diamidino-2-phenylindole (DAPI) (1 µg/ml; Sigma-Aldrich) for 5 min ...
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bioRxiv - Molecular Biology 2022Quote: Time-lapse fluorescence microscopy (TLFM) was performed on B04A plates with an ONIX flow control system (Merck-Millipore) (24 ...
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bioRxiv - Neuroscience 2022Quote: ... washed again three times in TBSt and signal was developed with p-nitrophenyl phosphate (pNPP Sigma Cat #N2765), the color reaction being quantified at 405nm.
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bioRxiv - Plant Biology 2022Quote: ... Transparent leaf segments were washed three times in 150 mM K2HPO4 (pH 9.6) and stained with 0.02 % aniline blue (cat. no: B8563-500ML; Sigma) in 150 mM K2HPO4 (pH 9.6) ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue sections were washed three times in PBS/0.3% Triton X-100 (PBS-T; Sigma, St. Louis, MO), and incubated in 10% normal goat serum/PBS/0.3% Triton X-100 (NGST ...
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bioRxiv - Neuroscience 2023Quote: ... After washing three times with Dulbecco’s Phosphate Buffered Saline with calcium and magnesium (DPBSCa2+Mg2+; Sigma-Aldrich, D8662), 5 µg/ml laminin (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... the slices were washed two times with Tris buffer (TB, 50 mM Tris, pH 7.6, Sigma-Aldrich, Germany), mounted on Superfrost slides (Superfrost plus ...
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bioRxiv - Plant Biology 2023Quote: ... Beads were washed 5 times with immunoprecipitation wash buffer (GTEN extraction buffer with 0.3% v/v IGEPAL (SIGMA)) and resuspended in 60 μl of SDS loading dye ...
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bioRxiv - Cell Biology 2024Quote: ... then washed three times with 1xPBS and permeabilized with 0.5 % Triton-X100 (Sigma-Aldrich, St. Louis, MO, USA) in 1xPBS for another 5 min ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed three times with washing buffer (PBS + 0.2% Tween 20) and incubated in hematoxylin (Sigma Aldrich) for 3 min ...
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bioRxiv - Molecular Biology 2022Quote: Eluate from in-lysate crosslinking experiment was mixed with four times ice-cold Acetone (EMSURE®, Sigma Aldrich) and NaCl (to a final concentration of 150 mM ...
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bioRxiv - Immunology 2022Quote: ... After the last step the plates were washed five times with TBS/0.05% Tween-20 and developed with a solution of 1% 3,3’,5,5’-tetramethylbenzidine (Sigma-Aldrich), 0.01% hydrogen peroxide ...
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bioRxiv - Neuroscience 2022Quote: ... The tests were performed three times pre- and 30 min post-injection of CNO (5.0 mg/kg body weight, i.p.; Sigma), and the average speed was calculated ...
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bioRxiv - Microbiology 2022Quote: ... Major peaks were identified by retention time relative to a Bacterial Acid Methyl Ester (BAME) standard (Millipore-Sigma) and a 37 FAME standard (Supelco) ...
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bioRxiv - Microbiology 2022Quote: ... Major peaks were identified by retention time relative to a Bacterial Acid Methyl Ester (BAME) standard (Millipore-Sigma) and a 37 FAME standard (Supelco) ...
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bioRxiv - Microbiology 2024Quote: ... spotted lipids were migrated three times sequentially in a running solution of 50:1 petroleum ether (Sigma Aldrich) and ethyl acetate (Sigma Aldrich) ...
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bioRxiv - Molecular Biology 2024Quote: ... were washed three times with 3 ml of water using Amicon Ultra-4 (3 kDa cut-off, Millipore) (centrifugation at 4°C ...
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Constitutive and conditional epitope-tagging of endogenous G protein coupled receptors in DrosophilabioRxiv - Neuroscience 2023Quote: ... brains were washed three times in PBS with 0.5% Triton X-100 (Millipore Sigma, Cat#X100, Burlington, MA) (PBST ...
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bioRxiv - Developmental Biology 2024Quote: ... PFA was removed and samples were washed 3 times with PBST (RNAse-free PBS and Tween20, Sigma Aldrich) with incubation times of 15 minutes ...
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bioRxiv - Immunology 2024Quote: ... Animals were then harvested by washing with M9 multiple times before RNA was isolated using TriReagent (Sigma-Aldrich), column purified (Qiagen) ...
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bioRxiv - Biophysics 2023Quote: ... The samples were washed five times with extraction buffer and then immunoblotted with anti-Cherry antibodies (Sigma-Aldrich).
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bioRxiv - Cell Biology 2023Quote: ... cells were washed five times in ice-cold DPBS and lysed in 6 M guanidine hydrochloride (Sigma-Aldrich) in 10 mM Tris ...
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bioRxiv - Microbiology 2023Quote: ... At 2 hrs and 24 hrs post-infection (p.i.) the BMMs were rinsed 3 times with sterile PBS and lysed in PBS/0.1% sodium deoxycholate (Sigma), followed by serial dilution in sterile PBS and plating on TSAC plates for CFU enumeration ...
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bioRxiv - Microbiology 2023Quote: ... Cells were passaged three times in a complete medium supplemented with 1 μg/mL of puromycin (Sigma-Aldrich). The A549-ATG16L1-KD and the A549-shControl cells were both maintained in the presence of 1 μg/mL puromycin and 500 μg/mL geneticin ...
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bioRxiv - Immunology 2023Quote: ... the plates were washed 3 times and the reaction was developed with 100uL p-Nitrophenyl phosphate (PNPP, Sigma; N-9389 ...
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bioRxiv - Immunology 2023Quote: ... Slides were washed three times for 5 min each in PBS including 0.1% Tween 20 (Sigma-Aldrich, P1379). Slides were blocked with 5% normal goat serum (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... added at the time of treatments and labeled by the click reaction with Azide Fluor 545 (Sigma Aldrich) as reported (14,19) ...
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bioRxiv - Cancer Biology 2023Quote: ... Wells were washed 3 times and 100 µL per well of SIGMAFAST OPD tablet (#P9187, Sigma-Aldrich, USA) solution was added ...
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bioRxiv - Immunology 2023Quote: ... Total RNA was extracted from the liver at the indicated time points after Plasmodium infection using TRIzol (Sigma), followed by DNase digestion and clean up with RNA Clean and Concentrator kit (Zymo Research) ...
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bioRxiv - Cell Biology 2023Quote: ... washed three times with phosphate buffer saline (PBS) containing 0.05% Tween 20 (PBST, Cat. No. P2287-100ML, Sigma), and then permeabilized with 0.5% Triton X-100 (Cat ...
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bioRxiv - Cancer Biology 2023Quote: ... PLP1CreERT2iDTR mice were injected intraperitoneally (i.p.) 2 times every other day with 100 mg/kg Tamoxifen (Sigma-Aldrich) dissolved in 100 µL MIGLYOL®812 (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... and chips were washed three times with sterile water before being coated with 50µl of polyethyleneimine (PEI, Sigma). Chips were placed back in the incubator for one hour ...