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Citations for Millipore Sigma :
2801 - 2850 of 10000+ citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The purified proteins were concentrated by ultracentrifugation (Amicon Ultra-15, Millipore) to ~15-20 mg/ml and stored in 5 mM bis-tris propane–HCl (BTP-HCl) ...
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bioRxiv - Biochemistry 2020Quote: ... Eluted protein was concentrated using 100-kDa cut off concentrator (Millipore) and further purified by Superose 6 increase (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... mouse anti-Alzheimer Precursor Protein (APP) 22C11 (1:100, MAB348, Millipore), and mouse anti-β-Tub III/Tuj 1 (1:4000 ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were reconstituted in sterile DMSO (Millipore-Sigma, Burlington, MA, USA) to stock concentrations of 10 mM ...
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bioRxiv - Neuroscience 2020Quote: ... Bound DNA and proteins were eluted with 16 mM biotin (Sigma) in water (at pH 7.0 ...
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bioRxiv - Biophysics 2021Quote: ... Crosslinked protein was concentrated using 100-kDa cut-off concentrator (Millipore) and further purified by Superose 6 increase (GE Healthcare ...
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bioRxiv - Cancer Biology 2021Quote: ... the protein was transferred to a methanol-activated PVDF membrane (Millipore) at 25 V for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... total protein was detected by staining with Ponceau S (Sigma-Aldrich). Membranes were blocked for 1 hour at room temperature in 5% milk or 5% BSA in Tris-Buffered-Saline-0.1% Tween-20 (TBST) ...
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bioRxiv - Cell Biology 2021Quote: ... Immunoreactive proteins were visualized by Immobilion western chemiluminescence substrate (Merck-Millipore). Densitometry was carried out by FIJI.
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bioRxiv - Cell Biology 2021Quote: ... Protein quantification was done using the Bradford colorimetric assay (Sigma B6916). 20µg of proteins were separated run on a 10% SDS-PAGE gel ...
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bioRxiv - Cancer Biology 2020Quote: ... at 1 ug/ml or control protein Bovine Serum Albumin (Sigma) at 1 ug/ml in PBS were coated onto flat bottom ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Resultant protein concentrations were determined using a Bradford assay (Sigma-Aldrich) and purity of the protein preparation determined by SDS-PAGE followed by silver staining ...
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bioRxiv - Pathology 2021Quote: ... Proteins from the resulting gels were transferred to PVDF membranes (Millipore), and analyzed by western blotting with relevant antibodies ...
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bioRxiv - Biophysics 2021Quote: ... The recombinant proteins were first purified by Ni2+-affinity column (Novagen) under native conditions and subsequently in-gel cleavage by TEV protease was conducted ...
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bioRxiv - Plant Biology 2020Quote: ... Purified protein was concentrated using an Amicon Filter 3K (#UFC500324, Millipore). In order to avoid any interference with downstream procedures ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were detected using Immobilon Western Chemiluminescent HRP substrate (Millipore MA) as per the manufacturer’s instructions.
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bioRxiv - Biochemistry 2020Quote: ... proteins were buffer exchanged in centrifugal concentrators (Amicon Ultra, EMD Millipore) to buffer A plus 300 mM NaCl and 20 mM imidazole ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were then transferred to a PVDF membrane (Merck Millipore) using a semi-dry western blot transfer system set to a constant current of 200 mA for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... Protein concentrations were determined using the Bradford Reagent (Sigma, Cat# B6916), SDS-PAGE densitometry ...
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bioRxiv - Biochemistry 2020Quote: ... and the protein inhibitor mixture was from Sigma (catalog no. S8830). Leupeptin ...
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bioRxiv - Biochemistry 2020Quote: ... The protein bands were transferred to PVDF membranes (Millipore, Cat# PVH00010). After being blocked with 5% non-fat dry milk ...
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bioRxiv - Biochemistry 2020Quote: ... the protein was concentrated by 50-kDa cut-off Centricon (Millipore) and performed size exclusion chromatography (SEC ...
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bioRxiv - Plant Biology 2020Quote: ... The immunoprecipitated proteins were incubated with 1 μg MBP (Sigma-Aldrich) in kinase buffer (25 mM Tris-HCl ...
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bioRxiv - Synthetic Biology 2021Quote: ... Proteins were then transferred onto a PVDF membrane (Milipore Sigma, IPFL00010) using the Biorad Trans-Blot Turbo system in accordance with the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and proteins were eluted with an excess of FLAG peptide (Sigma) or HA peptide (Sigma ...
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bioRxiv - Systems Biology 2020Quote: ... The purified proteins were concentrated with Amicon 10K filters (Millipore Sigma) and their concentration assessed using Coomassie and BCA assays (Thermo Fisher Scientific).
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bioRxiv - Plant Biology 2021Quote: ... proteins were blotted onto a Protan nitrocellulose membrane (Sigma-Aldrich, 10401396). Rabbit anti-RGA polyclonal antibody (Agrisera ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were eluted with 50μL of 100μg/mL 3xFLAG peptide (Sigma) in 1x Tris-buffered saline ...
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bioRxiv - Microbiology 2021Quote: ... cell protein expression was synchronized with cycloheximide (Sigma, 0.1 mg/mL), followed by membrane protein cross-linking with 3,3’-dithiobis(sulfosuccinimidyl propionate ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were detected using rabbit polyclonal anti-GAPDH (Sigma Aldrich, G9545), mouse monoclonal anti-SARS-CoV-2 Nucleocapsid [1C7] and mouse monoclonal anti-SARS-CoV-2 Spike [2B3E5] protein (a kind gift by Dr ...
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bioRxiv - Microbiology 2021Quote: ... proteins were transferred onto the PVDF membrane (Immobilon-FL, Merck-Millipore). After the electrotransfer ...
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bioRxiv - Physiology 2021Quote: ... breast cancer resistance protein (Bcrp-1:100; Merck Millipore, Massachusetts, USA) or Abcg1 (1:200 ...
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bioRxiv - Microbiology 2021Quote: ... Post-spin lysates were then precleared using protein A-agarose (Sigma) for 1 h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were detected using rabbit polyclonal anti-GAPDH (Sigma Aldrich, G9545), mouse monoclonal anti-SARS-CoV-2 Nucleocapsid [1C7] and mouse monoclonal anti-SARS-CoV-2 Spike [2B3E5] protein (a kind gift by Dr ...
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bioRxiv - Cell Biology 2021Quote: ... Protein extractions were electroblotted on a polyvinylidene fluoride (PVDF) membrane (Millipore). Membranes were blocked with 5% nonfat dry milk in TBST for 1h and probed with 1:1000 anti-p53 (cst ...
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bioRxiv - Cell Biology 2021Quote: ... Protein signals were developed with Immobilon Western Chemiluminescent HRP Substrate (Millipore) and imaged with ChemiDoc™ Imaging Systems (Bio rad).
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were transferred to an Immobilon-P PVDF membrane (EMD Millipore). Primary and secondary antibodies were added in order ...
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bioRxiv - Cell Biology 2021Quote: ... The proteins were transferred to an immobilon-P membrane (EMD Millipore). For protein detection ...
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bioRxiv - Biophysics 2021Quote: ... protein was expressed in N5052 minimal medium24 using 15NH4Cl (Sigma-Aldrich). For 13C,15N labelling ...
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bioRxiv - Biophysics 2020Quote: ... resolved proteins were transferred onto Immobilon-P PVDF membranes (Millipore Sigma) using a Bio-Rad Trans-blot® semi-dry transfer cell ...
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bioRxiv - Biophysics 2021Quote: ... the proteins were purified using His-Select Nickel agarose resin (Sigma) and anion exchange chromatography (Source Q ...
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bioRxiv - Biophysics 2020Quote: ... Proteins were expressed via BL21(DE3) Rosetta Escherichia coli cells (Novagen). Full-length PRC1 was expressed for 3.5 hours at 18°C after induction with 0.5 mM IPTG ...
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bioRxiv - Biophysics 2020Quote: ... Eluted protein was concentrated using 100-kDa cut off concentrator (Millipore) and further purified by Superose 6 increase (GE Healthcare ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lysate protein bands were normalized to GAPDH (EMD Millipore, MAB347) at 1:2000 dilution ...
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bioRxiv - Cancer Biology 2021Quote: ... Resolved protein was transferred to PVDF membranes (Millipore, Billerica, MA, USA) using a Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, Darmstadt, Germany) at 14V constant in a semi-dry transfer machine ...
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bioRxiv - Cell Biology 2020Quote: Proteins were transferred onto a PVDF membrane (Immobilon-P, Merck Millipore) using wet transfer ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were electroblotted to 0.45μm PVDF membranes (Imobilon-P, Merck Millipore, IPVH00010 ...
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bioRxiv - Cell Biology 2021Quote: ... the electrophoresed proteins were transferred from gel to PVDF membranes (Millipore) following with incubation with primary and secondary antibodies and finally bands were detected ...
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bioRxiv - Cell Biology 2021Quote: ... serine/threonine protein phosphatase inhibitors (Phosphatase inhibitor Cocktail 3, Sigma-Aldrich) and 1 mmol/L Na3VO4 ...