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Citations for Millipore Sigma :
3051 - 3100 of 10000+ citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... was immobilised on MagnaChIP™ Protein A magnetic beads (Sigma-Aldrich). Both the wildtype as well as the med25-1 mutant were used for the analysis under normoxic and hypoxic conditions ...
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bioRxiv - Immunology 2023Quote: ... Proteins were transferred to an Immobilon-FL membrane (Sigma Aldrich, USA) using a humid transfer device and 350 mA ...
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bioRxiv - Physiology 2024Quote: ... Protein content was determined using a Bradford assay (Sigma-Aldrich, USA).
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bioRxiv - Cell Biology 2022Quote: ... Protein extracts were mixed with 2x Laemlli sample buffer (S3401, Sigma) and heated at 95°C for 10 minutes prior to SDS-PAGE ...
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bioRxiv - Cell Biology 2022Quote: Protein lysates were collected using Radio-Immunoprecipitation Assay (RIPA) buffer (Sigma), supplemented with protease inhibitor (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... After protein quantification by the bicinchoninic acid colorimetric assay system (Sigma), 1mg protein samples were used for HDAC3 immunoprecipitation using four microliters of HDAC3 antibodies (Cell Signalling ...
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Co-stimulatory molecules decide T cell fate through regulations of their invigoration and impairmentbioRxiv - Molecular Biology 2022Quote: ... and the resolved proteins were then transferred to PVDF membrane (Millipore). The membrane was blocked using 5% skimmed milk and then immunoblotted with primary antibodies at 4°C overnight ...
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bioRxiv - Biochemistry 2022Quote: ... the proteins were digested with trypsin (TPCK Treated; Sigma; Cat. # 4352157). iTRAQ labeling of the peptides in each sample was performed using the iTRAQ Reagents Multiplex kit (AB Sciex) ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was concentrated in a 50,000 MWCO Amicon Concentrator (Millipore) to <1 mL and injected onto a Superdex™ 200 10/300 GL Increase size-exclusion column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... The eluted protein was treated with 10 U PreScission protease (Sigma) at 4°C for 2 hours ...
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bioRxiv - Biochemistry 2022Quote: ... the proteins were concentrated using a 10 K Amicon (Merck-Millipore), flash-frozen in liquid nitrogen and stored at −80°C until use.
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bioRxiv - Cell Biology 2022Quote: ... and phosSTOP if phosphorylated proteins were being investigated (Sigma-Aldrich, 04906845001)] ...
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bioRxiv - Cell Biology 2022Quote: ... After protein transfer membranes were incubated in Ponceau solution (Sigma-Aldrich) to confirm even transfer ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentrations were determined by Bradford assay kit (Sigma-Aldrich, B6916). Lysates were mixed with 5 × sample buffer (250 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... stained with 1:200 mouse anti-ubiquitinylated proteins (Sigma #04-263) overnight at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were electro-transferred to a polyvinylidene fluoride membrane (Millipore) using Turbo-Blot Turbo transfer system (settings ...
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bioRxiv - Microbiology 2022Quote: ... HA-tagged proteins were immunoprecipitated with anti-HA agarose beads (Sigma) and processed for Western blotting as previously described.
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bioRxiv - Microbiology 2022Quote: ... affinity purified IcsAp protein was mixed with 0.1 mM DSP (Sigma) in PBS and incubated at room temperature for 30 min ...
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bioRxiv - Synthetic Biology 2022Quote: ... resuspended and lysed in 200 μL Bugbuster protein extraction reagent (Millipore). Following another centrifugation ...
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bioRxiv - Plant Biology 2022Quote: ... the proteins were transferred on a PVDF membrane (Immobilon-P, Millipore) using a wet blotting system ...
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bioRxiv - Plant Biology 2022Quote: ... samples were incubated with 25 μl of protein A beads (Millipore) for 2 h in cold room (100 μL were collected as input) ...
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bioRxiv - Neuroscience 2022Quote: ... Resulting protein lysates were precipitated with trichloroacetic acid (TCA) (Sigma, T0699). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-phosphoepitope SR proteins clone1H4 (Millipore, cat#MABE50, 1:2,000 dilution) were used as primary antibodies ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... as measured by a BCA protein assay kit (Sigma-Aldrich, USA).
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bioRxiv - Plant Biology 2022Quote: ... Proteins were electro-transferred onto Immobilon TM PVDF membranes (Millipore, USA). The membrane was incubated with rabbit anti-C ...
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bioRxiv - Molecular Biology 2023Quote: ... resolved proteins were transferred onto Immobilon-P PVDF membranes (Millipore Sigma) using a Bio-Rad Trans-blot® semi-dry transfer cell ...
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bioRxiv - Plant Biology 2023Quote: ... Protein extracts were then reduced with 10 mM dithiothreitol (D9779, Sigma), followed by alkylation with 30 mM Iodoacetamide (I1149 ...
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bioRxiv - Biochemistry 2023Quote: ... the proteins were transferred to Immobilon-P transfer membranes (Sigma-Aldrich) using the Trans-Blot® TurboTM Transfer System (Bio-Rad Laboratories) ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were extracted as whole cell lysate using trichloroacetic acid (Sigma) and glass beads as described before87.
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bioRxiv - Developmental Biology 2022Quote: Proteins were transferred to 0.45 μm Immobilon-P PVDF membrane (Millipore) in transfer buffer (25 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... For conditional induction of protein expression 1μg/ml of doxycycline (Sigma) was added to the culture media and incubated for 16-24h.
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bioRxiv - Cell Biology 2022Quote: ... Transfer of protein on activated PVDF membranes (EMD-Millipore, cat#IPVH07850) were performed overnight at 4°C at 50mA ...
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bioRxiv - Cell Biology 2022Quote: ... Protein quantification was performed by the bicinchoninic acid method (Sigma-Aldrich) for normalization of lipolysis data.
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bioRxiv - Cancer Biology 2023Quote: Protein extracts were prepared by lysing cells in RIPA buffer (Sigma) supplemented with a phosSTOP tablet (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2022Quote: ... The recombinant proteins were first purified by Ni2+-affinity column (Novagen) under native conditions and subsequently in-gel cleavage by TEV protease was conducted ...
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bioRxiv - Biophysics 2023Quote: ... The protein was concentrated using an Amicon centrifugal concentrator (EMD Millipore) as necessary ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein supernatants were collected and quantified by Bradford assay (Sigma Aldrich).
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bioRxiv - Cell Biology 2023Quote: ... Resolved proteins were transferred overnight into a PVDF membrane (0.22µm, Millipore) followed by 30 min incubation in blocking buffer (TBS ...
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bioRxiv - Cell Biology 2023Quote: ... the proteins were transferred to a polyvinylidene fluoride membrane (Merck Millipore), followed by blocking in 2% BSA containing 50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were then filtered through a 10kDa protein filter (Millipore) by centrifugation at 12,000g ...
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bioRxiv - Developmental Biology 2023Quote: ... 103 units/mL ESGRO recombinant mouse LIF protein (Millipore Sigma, ESG1107), 2 μM PD0325901 (Tocris ...
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bioRxiv - Developmental Biology 2023Quote: ... The samples were lysed with Cytobuster™ Protein Extraction Reagant (Millipore) supplemented with HALT protease inhibitor cocktail ...
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bioRxiv - Immunology 2023Quote: PureProteome Protein A magnetic beads were obtained from EMD Millipore (LSKMAGA10). The RPMI-1640 media with L-glutamine ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were separated by 8% resolving gel containing trichloroethanol (TCE; Sigma) to visualize proteins (Ladner et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were concentrated using an ultracel-100 membrane filter unit (Millipore) and then the concentration of NaCl was adjusted to 100 mM ...
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bioRxiv - Biochemistry 2023Quote: ... filtered through a 0.45 mM low protein-binding PVDF filter (Millipore) and applied to a 1 mL StrepTrap HP column pre-equilibrated in Strep-start buffer (Cytiva) ...
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bioRxiv - Physiology 2022Quote: ... proteins were transferred to PVDF membranes (Merck Millipore, IEVH00005, Tokyo, Japan), blocked with 2.5% non-fat milk in PBS containing 0.1% Tween 20 for 1 h ...
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bioRxiv - Physiology 2023Quote: ... The lysosomal protein degradation inhibitor chloroquine (ChQ; 100 μM; Sigma-Aldrich) was added to the cell culture medium 30 minutes prior to ghrelin treatment for 6 hours.
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bioRxiv - Biochemistry 2023Quote: ... The protein was concentrated in a 50,000 MWCO Amicon Concentrator (Millipore) to <1 mL and injected onto a Superdex™ 200 10/300 GL Increase size-exclusion column (GE Healthcare ...
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bioRxiv - Bioengineering 2023Quote: ... Then protein signal was detected using Immobilon Western HRP substrate (Millipore) and Fusion FX6 Imaging System (Vilber ...