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Citations for Millipore Sigma :
2751 - 2800 of 10000+ citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Flag-protein complexes were eluted using Flag peptide (Millipore, Cat # F3290) at 150 µg/ml by incubating the beads at room temperature for 30 minutes ...
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bioRxiv - Biophysics 2020Quote: ... FN3 proteins were expressed in Rosetta DE3 competent cells (EMD Millipore), lysed by sonication ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were transferred onto an Immobilon-P membrane (PVDF, EMD Millipore) using a Bio-Rad system with a 10% Tris-Glycine and 10% methanol transfer buffer diluted in distilled and deionized water ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were resuspended in 100 mM TEAB buffer (triethylammonium bicarbonate, Sigma) and then digested by trypsin (Pierce MS grade ...
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bioRxiv - Cancer Biology 2021Quote: Proteins were transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, Darmstadt, Germany) using a BioRad transfer machine after being run through 10% SDS-polyacrylamide (SDS-PAGE ...
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bioRxiv - Cancer Biology 2021Quote: ... Resolved protein was transferred to PVDF membranes (Millipore, Billerica, MA, USA) using a Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Cancer Biology 2021Quote: ... proteins were detected using either Luminata Crescendo Western HRP Substrate (Millipore) or ECL Western Blotting Analysis System (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were then transferred to an Immobilon-P transfer membrane (Millipore) using a Trans-Blot Turbo Transfer system (BIO-RAD) ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration in the supernatant was measured using Bradford Reagent (Sigma), and equal amounts of protein were resolved by SDS-PAGE.
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bioRxiv - Cell Biology 2020Quote: ... Proteins were then transferred to PVDF membranes (Millipore, Burlington, MA, USA) and blotted with anti-phospho-Akt (Ser473 ...
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bioRxiv - Genetics 2021Quote: ... The expressed proteins were partially purified on Q sepharose (Sigma q1126) and the fractions containing the protein of interest were collected ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were transferred to 0.45 μm Immobilon-FL PVDF membranes (Millipore), and membranes were blocked in either 5 % BSA or Odyssey Blocking buffer (LI-COR Biosciences ...
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bioRxiv - Cell Biology 2020Quote: ... Separated proteins were transferred to PVDF-FL transfer membrane (Merck Millipore) at 40V for 90 min at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins were reduced with 1.5 µmol of DTT (Sigma-Aldrich, Canada) for 20 minutes at 70 degrees Celsius ...
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bioRxiv - Molecular Biology 2021Quote: ... The cells where then lysed with BugBuster protein extraction reagent (Novagen) for 30 min at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... rabbit anti-surfactant associated protein C (SFTPC, 1:1000, Millipore, AB3786), goat anti-podoplanin (PDPN ...
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bioRxiv - Immunology 2020Quote: A commercial protein-A affinity chromatography called PURE-1A (Sigma-Aldrich.) was used to purify anti-anti-SpA antibodies (Ab-3 ...
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bioRxiv - Developmental Biology 2020Quote: ... and protein concentration was determined using the Direct Detect spectrometer (Millipore). 20 µg of protein per sample was denatured at 70°C for 10 mins in 1× SDS Loading Dye (100 mM Tris pH 6.8 ...
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bioRxiv - Developmental Biology 2020Quote: ... the proteins were transferred onto a PVDF membrane (EMD Millipore, IPVH00010). The membranes were blocked with StartingBlockTM T20 (TBS ...
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bioRxiv - Immunology 2022Quote: Protein lysate was extracted using CelLytic B Cell Lysis Reagent (Sigma) following the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2022Quote: ... protein bands were visualized using an enhanced chemiluminescence detection kit (Millipore) and and the Syngene Pxi4 imaging system (Ozyme) ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were transferred by wet transfer to a PVDF membrane (Millipore) at 4°C overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... AID-tagged proteins were conditionally depleted using 250mM auxin (Sigma, I2886) stock dissolved in ethanol at a final concentration of 0.5mM ...
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bioRxiv - Molecular Biology 2021Quote: ... 40 µl of pre-washed protein A beads per sample (Sigma, equilibrated to lysis buffer as mentioned in the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... The proteins were transferred to Immobilon FL (EMD Millipore Corp IPFL00010) membrane in transfer buffer containing 10% methanol and 0.2% SDS ...
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bioRxiv - Neuroscience 2021Quote: ... and house-keeping protein GAPDH (1:500; Mouse; Sigma-Aldrich G8795) or β-actin (1:100 ...
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bioRxiv - Cell Biology 2022Quote: ... was used to transfer proteins to PVDF Immobilon membranes (Merck Millipore). Blocking and antibody incubation steps were performed in EveryBlot Blocking Buffer (BioRad) ...
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bioRxiv - Genomics 2020Quote: ... Eluted protein was concentrated by centrifugal ultrafiltration (Millipore Amicon Ultra, 10K), and loaded onto a pre-equilibrated HiLoad Superdex 75 16/60 column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... Digested glycans were cleaned using a PVDF protein-binding membrane (Millipore) and analyzed as described above.
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bioRxiv - Biochemistry 2020Quote: ... Proteins were transferred onto Immobilon-FL PVDF membranes (Millipore; Cat # IPFL00010), and nonspecific protein binding was blocked with 3% BSA in Tris-buffered saline for at least one hour at room temperature ...
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bioRxiv - Physiology 2020Quote: ... Tissue proteins were isolated by homogenization in RIPA buffer (Sigma-Aldrich) supplemented with cOmplete Mini protease inhibitor cocktail tablets (Roche Diagnostics ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted proteins were concentrated using Amicon Ultra filters (Millipore, Ireland), and confirmed by SDS-PAGE.
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bioRxiv - Plant Biology 2020Quote: ... Transferred proteins were probed with anti-tubulin (Sigma, 1:10,000 dilution) and anti-GFP (Takara Bio ...
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bioRxiv - Plant Biology 2020Quote: ... All proteins were expressed in BL21 Rosetta DE3 pLysS cells (Novagen) and purified using IMAC as described in detail previously41.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The total protein extracted was quantitated using Bradford Reagent (Sigma, USA). Bovine Serum Albumin (BSA ...
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bioRxiv - Bioengineering 2021Quote: ... mouse anti-glial fibrillary acidic protein (GFAP)–Cy3 (Sigma, 1:2000) to show astrocyte structure ...
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bioRxiv - Genetics 2020Quote: ... Proteins were immunoprecipitated using ANTI-FLAG® M2 Affinity Gel (Sigma) for 2 h at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins expression was detected using an enhanced chemiluminescence kit (EMD Millipore).
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bioRxiv - Immunology 2021Quote: ... proteins were buffer exchanged in centrifugal concentrators (Amicon Ultra, EMD Millipore) to buffer A plus 300 mM NaCl and 20 mM imidazole ...
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bioRxiv - Systems Biology 2021Quote: ... Stained proteins were detected using Immobilon Western Chemiluminescent HRP Substrate (Millipore) and image acquisition using a ChemiDoc Imaging system (Bio-rad) ...
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bioRxiv - Cell Biology 2020Quote: ... EZview™ Red Protein G Affinity Gel was from Sigma Aldrich. Click-iT™ EdU Cell Proliferation Kit for Imaging ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was concentrated using Amicon Ultra Centrifugal Filters (Merk Millipore). Selenomethionine derivated PrgL32-208 was purified following the same protocol with the exception that 0.5 mM tris(2-carboxyethyl)-phosphine (TCEP ...
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bioRxiv - Cell Biology 2021Quote: ... the membrane was blotted with anti-Protein A (1:10,000, Sigma), anti-HA (1:2000 ...
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bioRxiv - Biochemistry 2021Quote: ... before adding 15 µL of Protein A-Sepharose beads (Sigma-Aldrich) and allowing the incubation to continue for further16 h at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... the purified protein was concentrated using Amicon Ultra 50 k (Millipore).
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bioRxiv - Biochemistry 2021Quote: ... or protein A/G agarose beads (EMD Millipore, cat. no. IP05) for 2 hours at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant proteins were induced in Rosetta™ (DE3) Competent Cells (Novagen) with 0.02% (w/v ...
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bioRxiv - Biochemistry 2021Quote: ... the N protein was labelled with ATTO 488 dye (Sigma Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: Heterologous protein expression was achieved using pET28b(+) (EMD Millipore, Burlington, MA), in which open reading frames for the enzymes were cloned into BamHI/XhoI doubly digested vector ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins from peak fractions were concentrated using centrifugal ultrafiltration devices (Millipore) to final concentrations of ∼1 mg/ml (RBD-8h proteins) ...