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Citations for Millipore Sigma :
3001 - 3050 of 10000+ citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... proteins were transferred Immobilon-E PVDF membrane (Merck Millipore, Tullagreen, Ireland) and AGAL was detected by monoclonal anti-Galactosidase alpha antibody (# ab168341 ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were transferred to a PVDF membrane (Immobilon-P, Millipore, IPVH00010) at 100 mA overnight ...
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bioRxiv - Bioengineering 2023Quote: ... protein was extracted by using RIPA lysis buffer (20-188, Millipore) supplemented with cOmplete Mini protease and phosphatase inhibitors tablets (Roche) ...
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bioRxiv - Biophysics 2024Quote: ... The protein was concentrated with Amicon Ultra-15 centrifugal filters (Millipore) and buffer exchanged to storage buffer (20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2024Quote: ... the proteins were transferred onto PVDF membranes (Millipore, Billerica, MA, USA), the membranes were quenched and proteins were detected using specific antibodies ...
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bioRxiv - Genomics 2024Quote: ... The protein concentration was measured with the Bradford Assay (Sigma-Aldrich). Aliquots of the extracts were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes (Amersham/GE Healthcare) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was concentrated using Amicon Ultra centrifugal filter units (Merck Millipore). Protein was then aliquoted ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins in the gel bands were reduced with dithiothreitol (DTT) (Sigma) and alkylated by iodoacetamide (IAA ...
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bioRxiv - Biochemistry 2024Quote: ... Eluted protein was concentrated using Amicon Ultra Centrifugal Filter units (Millipore) and further purified by size exclusion chromatography using a Superdex 75 prepgrade column (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was blotted off with filter paper (Sigma–Aldrich, WHA1001110), and immediately after blotting ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins then transferred to 0.45 µM Immobilon-FL PVDF (Millipore Sigma) after methanol activation ...
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bioRxiv - Neuroscience 2024Quote: ... The proteins were transferred to a PVDF membrane (Millipore, catalog #IPVH00010) at 100V for 30 min in transfer buffer (20% methanol ...
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bioRxiv - Physiology 2024Quote: ... Protein was visualized with Immobilon Western Chemiluminescent HRP Substrate (Millipore, #WBKLS0500) and imaged on a ChemiDoc (BioRad) ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins bound to anti-Flag M2 affinity beads (Sigma-Aldrich) were recovered from the supernatants and washed by Flag wash buffer (50 mM NaPi (pH 7.5) ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred onto Immobilon-P PVDF membranes (Millipore, Darmstadt, Germany) in 1× Tris Glycine with 20% ethanol ...
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bioRxiv - Cell Biology 2024Quote: ... and the protein concentration was determined with Bradford reagent (Sigma-Aldrich). Lysates were subsequently denatured in Laemmli sample buffer for 5 minutes at 95°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were subsequently transferred to a 0.45 µm PVDF membrane (Millipore). Primary antibodies were used at the following dilutions in 5% Blotting-Grade Blocker (BioRad) ...
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bioRxiv - Developmental Biology 2023Quote: ... protein lysates were prepared in 1× RIPA buffer (Millipore, #20-188) from Drosophila head tissue expressing UAS-FLAG-Dx ...
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bioRxiv - Genetics 2024Quote: ... the proteins were transferred onto a nylon membrane (Immobilon-P, Millipore) and incubated with primary antibodies in a blocking buffer (1ξ PBS ...
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bioRxiv - Synthetic Biology 2023Quote: ... and pellets were resuspended in BugBuster® protein extraction reagent (Millipore). Protein from the lysed cell suspension was then extracted by chloroform/methanol precipitation ...
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bioRxiv - Immunology 2023Quote: ... FLAG-tagged proteins were immunoprecipitated with anti-FLAG M2-agarose (Sigma), following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was determined by a Bradford assay (Sigma Aldrich; B6916). Proteins were denatured in NuPAGE™ LDS Sample Buffer (4X ...
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bioRxiv - Biochemistry 2023Quote: The lysate was pre-cleared with ∼20µL Protein G agarose (Sigma) per 1mL of lysate at 4°C for 30min ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit anti-pro-surfactant protein C (SFTPC, 1:1000, AB3786, Millipore), goat anti-SOX9 (SOX9 ...
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bioRxiv - Immunology 2023Quote: ... injection of 30 μg of OVA protein (Grade V; Sigma-Aldrich) adsorbed to 2 mg of aluminum hydroxide (alum ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were blotted onto Immobilon-P PVDF membrane (0.2 µm, Millipore) using a Trans-Blot Semi-Dry transfer Cell (BioRad ...
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bioRxiv - Cell Biology 2023Quote: ... Protein bands were detected with Immobilon Western HRP Substrate (Merck Millipore) and images captured with a CCD camera and the Bio-Rad ChemiDoc MP System (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... anti-prosurfactant protein C (proSP-C, Millipore Sigma AB3786, RRID: AB_91588) was used at 1:4,000 with microwave antigen retrieval as described above ...
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bioRxiv - Neuroscience 2023Quote: ... proteins were visualized using chemiluminescence (Immobilon ECL western HRP substrate, Millipore). Densitometric analysis was carried out using the Image J software ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant fusion protein was purified with Glutathione Sepharose beads (Sigma). Definite probes were designed for different cis-elements and their mutated versions (Table S3) ...
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bioRxiv - Plant Biology 2024Quote: ... Protein extracts were then reduced with 10 mM dithiothreitol (D9779, Sigma) for 5 min at 95 °C ...
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bioRxiv - Microbiology 2024Quote: ... Protein was concentrated with Amicon® Ultracel ® 3K filters (Millipore) for immunization of rabbits by Covance (Denver ...
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bioRxiv - Developmental Biology 2024Quote: ... Recombinant protein was produced in Escherichia coli strain BL21(DE3) (Novagen). The cells were grown in LB medium at 37°C and the protein expression was induced at OD600 of 0.7 with 0.2 mM IPTG (Isopropyl β-d-1-thiogalactopyranoside ...
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bioRxiv - Physiology 2024Quote: ... Protein concentration was determined using Bradford assay (Sigma, Cat. No. B6916). 10 μg from each sample was separated in 10% SDS-PAGE gels ...
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bioRxiv - Physiology 2024Quote: ... Protein concentration was determined using Bradford assay (Sigma, Cat. No. B6916). 35 μg of protein were electrophoretically separated using 8% SDS-PAGE and transferred to PDVF membranes for 2.5 h at 200 mA per membrane using a semi-dry system at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Target proteins were visualized using Immobilon Western Chemiluminescent HRP substrate (Millipore).
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bioRxiv - Microbiology 2023Quote: ... Protein concentration was determined by the Bradford method (Sigma-Aldrich, B6916). 50 μL magnetic beads (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: ... Purified proteins were concentrated using Amicon Ultra 3K MWCO filters (Millipore) in storage buffer (50 mM Tris-HCl pH 7.6 ...
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bioRxiv - Biophysics 2023Quote: ... Protein expression was performed in Bl21 (DE3) pLys competent cells (Novagen) using an L.B ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were also lysed in BugBuster Protein Extraction buffer (EMD Millipore) for 30 min instead of sonication.
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bioRxiv - Biochemistry 2023Quote: ... 25 µg of protein was reduced with 2 mM dithiothreitol (Sigma) for 60 minutes at 55 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were then transferred to a methanol-activated PVDF membrane (Millipore) for 1 h at 100 V ...
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bioRxiv - Cell Biology 2023Quote: ... anti-Cell cycle control protein 50A (anti-CDC50A/TMEM30A, Sigma, AV47410), anti-Microtubule-associated protein 1 light chain B (anti-MAPLC3/LC3 ...
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bioRxiv - Physiology 2023Quote: ... Protein was visualized with Immobilon Western Chemiluminescent HRP Substrate (Millipore; #WBKLS0500) and imaged on a ChemiDoc (BioRad) ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were detected using the Immobilon Western Chemiluminescent HRP Substrate (Millipore) or SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... injection of 40 μg of OVA protein (Grade V; Sigma-Aldrich) adsorbed to 2 mg of aluminum hydroxide (alum ...
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bioRxiv - Biochemistry 2023Quote: ... Protein concentration was then measured using a Bradford assay (#B6916, Sigma), after which the proteins were diluted in Promega buffer (50 mM NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... Protein samples were then concentrated using Amicon 3-kDa concentrators (Millipore), and the purity of each protein was assessed by SDS-PAGE and further confirmed by Western blotting using commercial antibodies against the strep tag (Biobharati Life Sciences ...
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bioRxiv - Physiology 2023Quote: ... proteins were transferred to polyvinylidene difluoride (PVDF) membranes (Millipore, Burlington, MA) overnight ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred from the gel onto PVDF membranes (EMD Millipore) using standard methods ...