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Citations for Millipore Sigma :
2601 - 2650 of 7813 citations for rno mir 148b 3p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... the reaction buffer was exchanged to the lysis buffer five times by Amicon Ultra 10K (Merck Millipore). Then ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Time-lapse images were acquired during ototoxin lesions by application of 50 μM copper sulfate (Millipore Sigma) to the bath ...
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bioRxiv - Immunology 2023Quote: ... Plates were then washed four times with PBS-T and 4-nitrophenyl phosphate (pNPP, Sigma-Aldrich, 71768) substrate was added ...
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bioRxiv - Cell Biology 2023Quote: ... Time-lapse imaging was performed using an ONIX microfluidics perfusion system (CellASIC ONIX; EMD Millipore, Burlington, MA). A suspension of 50 μL of 40 × 106 cells/ml YE was loaded into Y04C plates for 5 s at 8 psi ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed a final time with PBS and 3,3’,5,5’- Tetramethyl benzidine (TMB) substrate (Sigma Aldrich) was added to each well ...
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bioRxiv - Cancer Biology 2022Quote: ... Wells were washed 3 times with PBS and cells were blocked with 10% goat serum (Sigma-Aldrich) for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Fixed neurons were rinsed three times with PBS and permeabilized in 0.1% Triton X-100 (Millipore Sigma) in PBS for 10min ...
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bioRxiv - Physiology 2023Quote: ... for developmental time points and 0.45-0.55 mg/ml collagenase and 0.05 mg/ml protease (Type XIV, Sigma) for adult animals ...
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bioRxiv - Physiology 2024Quote: ... Filters were washed three times with cold phosphate-buffered saline containing MgCl2 and CaCl2 (PBS; Sigma, D8662), filters were excised with a razor blade ...
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bioRxiv - Bioengineering 2023Quote: ... Hydrogels were washed 6 times with sterile PBS and kept in fibronectin solution (20 μg/ mL; Sigma) for 1 h at room temperature.
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bioRxiv - Microbiology 2023Quote: ... Oocysts were then washed 4 times in DPBS containing 1% (wt/vol) bovine serum albumin (BSA) (Sigma) before resuspending with 1% BSA ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed three times with PBS and blocked with 3% bovine serum albumin (A8412, Sigma-Aldrich) in PBS for 30 minutes at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... membranes were washed 3x every 10 minutes for a total of 3 times before ECL (Millipore #WBLUF0500) was added and imaged by chemidoc+ system (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... washed three times with a buffer containing PBS and 0.15% Triton X-100 (Sigma, Cat#X100-500ML). The resulting pellets were resuspended in 20 μl of 1X NuPAGE LDS sample buffer (Thermo Scientific Cat#NP007 ...
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bioRxiv - Biophysics 2023Quote: ... and rinsed four times with 0.1 M phosphate buffer pH 7.4 and 0.05 M glycine (Sigma-Aldrich). After washing ...
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bioRxiv - Immunology 2024Quote: ... and subsequently transduced two times with viral supernatant in the presence of 6 ug/ml polybrene (Sigma) and 50 U/ml IL-2 ...
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bioRxiv - Cancer Biology 2024Quote: The cells on coverslips were first washed three times with Phosphate-Buffered Saline (PBS, Sigma-Aldrich, P4417) before being fixed with a 4% (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... wells were washed 3 times with PBS supplemented with 0.05% (v/v) Tween®20 (Sigma-Aldrich) and 3 times with PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed three times with 1X PBS and then blocked using 1% bovine serum albumin (Sigma)/1X PBS solution for 40 minutes at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... cells were washed three times with PBS 1x and incubated with 0.01% sodium borohydride (Sigma Aldrich, 213462) for 7 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... the column was washed seven times according to procedure with 0.4 M NaCl (NaCl (EMD Millipore; 1.06404.1000) in DPBS ...
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bioRxiv - Microbiology 2024Quote: ... washed 3 times with 1x phosphate-buffered saline (PBS) then mounted in Fluoroshield histology mounting medium (Sigma). For each experiment ...
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bioRxiv - Microbiology 2024Quote: ... twice in Tris-HCl pH 7.5 and three times in 100 nM Triethylammonium bicarbonate buffer (Sigma Aldrich). The proteins on the beads were digested as described (Hubner et al. ...
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bioRxiv - Cell Biology 2020Quote: ... The PCR was performed with REDTaq ReadyMix (Sigma-Aldrich, cat# R2523) and loaded on a 1% agarose gel ...
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bioRxiv - Developmental Biology 2020Quote: ... The PCR-product was amplified with the KOD polymerase (Novagen; 71086) and purified with the QIAquick PCR Purification Kit (Qiagen ...
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bioRxiv - Neuroscience 2019Quote: cDNA was PCR-amplified using a JumpStart REDTaq ReadyMix (Sigma-Aldrich) and svr- ...
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bioRxiv - Cell Biology 2021Quote: ... The cap and lower third of 0.2 mL PCR tubes (Sigma) were removed to create wells for imaging extract12 ...
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bioRxiv - Microbiology 2021Quote: ... The colony PCR was performed with REDTaq ReadyMix (Sigma-Aldrich R2523) using the T7 promoter primer as forward and the T7 terminator primer as the reverse ...
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bioRxiv - Microbiology 2020Quote: ... PCR was carried out using KOD hot-start polymerase (EMD Millipore) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Quantitative PCR was performed with SybrGreen JumpStart Taq (Sigma-Aldrich, USA) in a RotorGene qPCR thermocycler (QIAGEN ...
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bioRxiv - Microbiology 2022Quote: ... PCR amplicons were ligated into the kanamycin resistant vector pET28b(+) (Novagen) using T4 ligase (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: ... VZV ORF31 was amplified by PCR with KOD Extreme (EMD Millipore) following the manufacturer’s instructions using the oligonucleotides [31]F56625-56645/[31]R59697-59717 ...
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bioRxiv - Neuroscience 2021Quote: ... PCR mix was prepared using gene-specific primers (0.6 μM, Sigma, primer sequences in the Appendix Table S1 and S2 ...
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bioRxiv - Microbiology 2020Quote: ... Primers for PCR amplification of fragments were ordered from Sigma-Aldrich. All primers and oligonucleotides used for cloning are listed in the table below.
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bioRxiv - Genetics 2022Quote: ... The PCR was performed with Thermococcus kodakaraenis (KOD) DNA polymerase (Novagen).
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bioRxiv - Developmental Biology 2019Quote: ... Genotyping was done using REDExtract-N-Amp PCR ReadyMix (Sigma-Aldrich) and respective primers ...
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bioRxiv - Immunology 2020Quote: ... All PCR products were amplified using KOD DNA polymerase (EMD Millipore) and purified by gel extraction (Clontech Libraries).
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bioRxiv - Synthetic Biology 2020Quote: PCR reactions were performed with Expand high Fidelity system (Sigma-Aldrich), Phusion DNA Polymerase (NEB) ...
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bioRxiv - Biochemistry 2020Quote: ... by PCR and inserted into bacterial expression plasmid pET23a (Novagen, USA) in order to construct either pET-hGFAT2 with and without HisTag ...
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bioRxiv - Neuroscience 2021Quote: ... The PCR product was digested and ligated into pET20b vector (Novagen) using AscI and XhoI ...
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bioRxiv - Biochemistry 2022Quote: ... The PCR fragment was cloned into the vector pET-11a (Novagen) using corresponding restriction sites ...
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bioRxiv - Cell Biology 2022Quote: ... Amplification was performed using REDExtract N Amp PCR ReadyMix (Sigma #R4775) according to the manufacturer protocol.
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bioRxiv - Microbiology 2023Quote: ... PCRs were done using the JumpStart Taq DNA polymerase (Sigma-Aldrich). Primer pairs were used to amplify by PCR flanking regions of approximately 500 bp downstream and upstream of the target genes using the appropriate genomic DNA as a template ...
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bioRxiv - Microbiology 2024Quote: ... PCR reactions were performed with KOD Polymerase (Novagen, San Diego, CA), and products were cleaned using the ZymoResearch Clean and Concentrator kit (Irvine ...
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bioRxiv - Molecular Biology 2023Quote: ... Inserts were acquired by PCR using KOD Hotstart Mastermix from NovaGen and purified from gel using Monarch DNA Gel Extraction kit from NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... We used the Expand Long Enzyme PCR system from Millipore Sigma to make small deletions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The PCR cleanup was then performed using Sephadex columns (Sigma-Aldrich). The purified product was sent to Macrogen Europe for sequencing.
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bioRxiv - Molecular Biology 2023Quote: ... Primers utilized to produce PCR fragments were purchased from Sigma-Aldrich.
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bioRxiv - Microbiology 2023Quote: All PCR primers used in this study were synthesized by Sigma and are indicated Supplementary Table S1.
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bioRxiv - Genomics 2023Quote: ... All PCRs were performed using KOD Hot Start DNA polymerase (Novagen) according to (46) ...