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Citations for Millipore Sigma :
3001 - 3050 of 7143 citations for Borrelia burgdorferi sensu stricto B31 P39 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... 15 μl of Magna ChIP protein A magnetic beads (16–661, Millipore, Molsheim, France) were added for 1h at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... cleaved protein was concentrated with a 30-kDa molecular mass cut-off concentrator (Millipore) by centrifugation for 5 min at 2,500 x g ...
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bioRxiv - Developmental Biology 2022Quote: ... and separated proteins were transfer to polyvinylidene fluoride (PVDF) membranes (EMD Millipore, Fisher Scientific). Total lane protein was then determined via Ponceau stain (0.1% Ponceau-S in 5% acetic acid ...
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bioRxiv - Cell Biology 2022Quote: ... Small molecules/ proteins were added to extracts as follows: 1 µM MLN8237 (Sigma-Aldrich) (or 0.5% DMSO ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were first precleared with PureProteome™ Protein A/G agarose suspension (Merck Millipore) for 1 h ...
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bioRxiv - Molecular Biology 2021Quote: ... The eluted protein was concentrated by ultrafiltration using an Amicon 50K filter unit (Millipore). Next ...
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bioRxiv - Molecular Biology 2021Quote: ... Eluted proteins were analyzed on SDS-PAGE and concentrated with Amicon Ultra concentrators (Millipore), aliquoted and stored at −80 °C ...
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bioRxiv - Biochemistry 2019Quote: ... Eluted protein was concentrated by a 30kDa centrifugal filter unit (Millipore Sigma Cat# ufc903024), and stored in 50% glycerol PBS at −20°C.
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bioRxiv - Immunology 2019Quote: ... mouse anti-MAP2 (microtubules associated protein 2) antibody (Cat. MAB378, Millipore, Boston, MA, USA), anti-ZIKV-E antibody (Cat ...
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bioRxiv - Physiology 2020Quote: ... The protein concentration was assessed using bicinchoninic acid assay (BCA, BCA1-1KT, Sigma Aldrich). A total of 10-40 μg of protein was loaded on 8-14% acrylamide gel ...
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bioRxiv - Cell Biology 2019Quote: ... The protein was then transferred on to a polyvinylidene fluoride membrane (PVDF) (Millipore, USA) using a semi-dry transfer system (Bio-Rad ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Variants were subcloned from the pEDA3 background into protein expression plasmid pET-29b(+) (Novagen) or into the constitutive expression plasmid pEDA2 (Wrenbeck et al ...
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bioRxiv - Plant Biology 2019Quote: ... The proteins in the gels were either visualized by silver staining (ProteoSilver; Sigma Aldrich) or transferred from the gel to a nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Plant Biology 2019Quote: ... 20µg of digested protein were desalted using 8µg C18 ZipTip (Millipore, Bedford, MA, USA). Approximately 1.5µg protein was injected into Acquity M-Class UPLC (Waters) ...
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bioRxiv - Plant Biology 2019Quote: ... 1 mL of protein extraction was applied to M5 anti-FLAG magnetic beads (Sigma). The beads were then boiled and the resulting fraction was run on a 12% SDS-PAGE gel ...
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bioRxiv - Microbiology 2020Quote: ... FLAG-tagged ANP32 proteins were detected using mouse anti-FLAG M2 primary antibody (Sigma), followed by goat anti-mouse Alexa Fluor 568 (Invitrogen) ...
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bioRxiv - Developmental Biology 2019Quote: For protein half-life analysis cells were treated with 10μg/ml cyclohexamide (Sigma Aldrich) for 2 ...
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bioRxiv - Immunology 2020Quote: ... Proteins were then transferred onto a PVDF membrane (Immobilon®-P Transfer Membrane, Millipore) via wet transfer and blocked in 5% skim milk/Tris-buffered saline (TBST ...
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bioRxiv - Cell Biology 2019Quote: ... The proteins were transferred on to a membrane (Millipore IVD HYBOND–C Extra membrane) by slot transfer under vacuum ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: We extracted the soluble protein from the damselflies using 0.1 M NaOH (Sigma-Aldrich) as a lysis buffer ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were transferred from SDS-PAGE gels to PVDF-membranes (Immobilon-P, Millipore 0.45µm) in transfer buffer (per 1 l ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant containing cleaved proteins were concentrated using a 50K MWCO concentrator (EMD Millipore) to 1 mL ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant containing cleaved proteins was concentrated using a 100K MWCO concentrator (EMD Millipore) to 500 µL and purified via size exclusion chromatography on a TSKgel G4000SWXL column (TOSOH Bioscience ...
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bioRxiv - Neuroscience 2019Quote: ... Protein synthesis was inhibited by including 20 μg/mL cycloheximide (Sigma-Aldrich, Cat #C4859) in the imaging medium ...
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bioRxiv - Biophysics 2019Quote: ... 15 μL diluted proteins were mixed with 1 μL 100x Sypro Orange (Sigma-Aldrich) and loaded in LightCycler 480 Multiwell Plate 96 (Roche ...
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bioRxiv - Plant Biology 2019Quote: ... Purified proteins were then concentrated using Amicon Ultra concentrators (molecular-weight cutoff 10,000; Millipore) and purity and structural integrity was assessed by SDS-PAGE and Right Angle Light Scattering (RALS) ...
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bioRxiv - Cancer Biology 2020Quote: ... and antibody/bead complexes isolated with Magna ChIPTM Protein A+G magnetic beads (Millipore). Complexes were washed ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were transferred onto a PVDF membrane (0.45 μm pore size, Immobilon-P, Millipore) using semidry transfer (Biorad) ...
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bioRxiv - Plant Biology 2020Quote: ... total proteins were incubated with 50 µL agarose conjugated with anti-Myc antibody (Sigma) for 2 h ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The protein content was determined using the Bradford reagent (Serva) with BSA (Sigma-Aldrich) as a standard (Bradford ...
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bioRxiv - Cell Biology 2019Quote: ... total protein content was stained using 0.1% (w/v) Ponceau S (Sigma-Aldrich, P3504) in 1% (v/v ...
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bioRxiv - Biochemistry 2019Quote: ... The delipidated NPC2 proteins were incubated with increasing concentrations of cholesterol (>99%) (Sigma Aldrich), in DMSO ...
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bioRxiv - Microbiology 2019Quote: ... and the soluble proteins were separated from the OMVs using an iodixanol (Optiprep, Sigma) density gradient ...
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bioRxiv - Microbiology 2019Quote: ... and the refolded protein was concentrated using 10,000 MWCO Amicon filter units (Millipore-Sigma). The concentrated material was then dialyzed using the same method described above ...
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bioRxiv - Microbiology 2019Quote: ... and the refolded protein was concentrated using 10,000 MWCO Amicon filter units (Millipore-Sigma). The concentrated material was then dialyzed using the same method described above ...
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were resolved by 10% SDS-PAGE and electrotransferred onto polyvinylidene fluoride membrane (Millipore) in transfer buffer (10 mM CAPS ...
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bioRxiv - Biochemistry 2020Quote: ... The purified proteins were then concentrated on either Amicon (Merck Millipore, MWCO 10 kDa) or Vivaspin (Sartorius ...
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bioRxiv - Bioengineering 2020Quote: ... using one of the following extracellular matrix (ECM) protein products: laminin (Laminin 111, Sigma L2020 ...
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bioRxiv - Pathology 2021Quote: ... proteins were transferred from the gel onto to a polyvinylidene fluoride membrane (Merck Millipore) via electroblotting at 120 V for 60 minutes in transfer buffer containing 20% methanol ...
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bioRxiv - Systems Biology 2021Quote: ... Proteins were detected using a monoclonal anti-FLAG M2 antibody (F3165, Sigma/Merck Millipore) and an HRP-conjugated polyclonal goat anti-mouse antibody (P0447 ...
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bioRxiv - Systems Biology 2021Quote: ... Proteins were detected using a monoclonal anti-FLAG M2 antibody (F3165, Sigma/Merck Millipore) and an HRP-conjugated polyclonal goat anti-mouse antibody (P0447 ...
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bioRxiv - Systems Biology 2020Quote: ... Total protein concentration was measured using bicinchoninic acid assay (BCA Kit, Sigma-Aldrich, USA).
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bioRxiv - Microbiology 2021Quote: ... Proteins were concentrated using Centriprep centrifugal filter devices (Millipore; molecular weight cutoff, 10 kDa) and further purified using size exclusion chromatography (SEC ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were reduced using 2.5 μL of dithiothreitol (200 mM DTT stock solution) (Sigma) at 90 °C for 20 min ...
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bioRxiv - Cell Biology 2021Quote: ... The immune complexes were incubated with protein A Sepharose beads (Sigma, St. Louis, MS) at 4°C for 1 hour ...
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bioRxiv - Biochemistry 2021Quote: ... Protein purity was assessed by 20% SDS-PAGE stained with Coomassie Brilliant Blue (Sigma). PageRule Unstained Broad Range Protein Ladder and PageRuler Plus Prestained Protein Ladder (both Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... and Flag-tagged FtsX proteins were detected by using ANTI-FLAG® antibody (Sigma) as previously described by Stamsås et al ...
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bioRxiv - Genetics 2021Quote: ... The immune-reactive proteins were detected using the Luminata Crescendo Western HRP substrate (Millipore).
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bioRxiv - Biochemistry 2020Quote: ... Bound proteins were eluted by 1X PBS with 10 mM reduced glutathione (Sigma Aldrich). Fractions containing VCA-NWASP were concentrated and dialyzed to 20 mM Tris (pH 7.4) ...
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bioRxiv - Microbiology 2021Quote: ... Target proteins were bound to 1 mL Ni-NTA His-Bind resin (Millipore Sigma) in gravity columns ...