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Citations for Millipore Sigma :
2951 - 3000 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... SAA and CTX-I were measured using the human SAA ELISA (Sigma-Aldrich) and Serum Crosslaps (CTX-I ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasma insulin was measured by enzyme-linked immunosorbent assay (ELISA) (Millipore, Burlington MA).
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bioRxiv - Immunology 2024Quote: ... 96-well ELISA plates were first coated overnight with poly-L-lysine (Sigma), then overnight with DNA from calf thymus (Sigma ...
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bioRxiv - Physiology 2023Quote: ... and plasma insulin (n=5) was analyzed by ELISA (EZRMI-13K, Sigma Aldrich), according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: Costar 96-well ELISA plates were coated with 10 μg of fibronectin (Sigma) for the fibronectin-binding assay and 10 μg of fibrinogen (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... Protein expression was induced by adding 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) (EMD Millipore Corp.; Cas: 367-93-1). Cells were then incubated for 18 to 20 hours at 20°C at 200 rpm ...
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bioRxiv - Cell Biology 2020Quote: ... 4-dinitrophenylhydrazine using protein carbonyl content assay kit according to the manufacturer’s instructions (Sigma, USA). The optical density of the adduct was examined at 375 nm ...
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bioRxiv - Molecular Biology 2020Quote: ... The immunoprecipitates were purified using Protein G Immunoprecipitation Kit (Cat. No. IP50, Sigma-Aldrich Inc.) and were separated on SDS-PAGE for western blotting using monoclonal antibodies against T18 as described above ...
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bioRxiv - Microbiology 2020Quote: The DRM and DSM fractions119 were separated using the CelLytic MEM Protein Extraction Kit (Sigma). 600 μl of lysis and separation buffer were added to 50 μl of resuspended membrane and incubated at 4°C overnight ...
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bioRxiv - Physiology 2021Quote: ... The remaining supernatant was precipitated using the Calbiochem Protein Extraction Kit (EMD Millipore, Billerica, MA) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: Tissue proteins were extracted according to kit instructions (Sigma Aldrich, R0278, St. Louis, Missouri, USA) and protein concentrations were routinely quantified by using a BCA kit (Beyotime ...
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bioRxiv - Cancer Biology 2020Quote: ... Firefly luciferase activity was normalized to protein content using Bicinchoninic Acid Kit from Sigma-Aldrich® and measured with using a luminometer (Centro XS3 LB960 ...
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bioRxiv - Cell Biology 2021Quote: RIP assays were performed using the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, USA) with the mouse anti-Ago2 antibody (Millipore ...
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bioRxiv - Cancer Biology 2022Quote: RIP was performed according to the Magna-RIPTM RNA-binding protein immunoprecipitation kit (Millipore/Merck) as previously described (Papoutsoglou et al ...
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bioRxiv - Molecular Biology 2022Quote: RNA immunoprecipitation was performed using the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, Billerica) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... and RIP assays were carried out with Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore) and anti-Flag antibody (Abcam ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentration in the extracts was measured using the QuantiPro BSA Assay Kit (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2023Quote: RIP experiment was optimized using the Magna RIP RNA-binding protein immunoprecipitation kit (Millipore, USA) in light of the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The total protein (μg/mL/OD600) was determined using a Lowry kit (Sigma-Aldrich, TP0300), and bovine serum albumin (BSA ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: The protein samples were separated by 8% and 12% SDS PAGE and were transferred to PVDF membranes (Merck Millipore, USA, Cat# ISEQ. 00010, LOT#R6PA4145H). The membranes w ere blocked with 5% skim milk for 3 h at 37 °C and were incubated for 14 h at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... a 40 μg aliquot of protein from each extract was separated by Tris-glycine sodium dodecyl-sulfate polyacrylamide gel electrophoresis (4% to 20%) and the protein bands were transferred onto polyvinylidene-fluoride membranes (Sigma-Aldrich, St. Louis, MO, USA), which were subsequently blocked with non-fat milk ...
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bioRxiv - Neuroscience 2022Quote: ... Frozen samples were sent to Eve Technologies and analyzed using the Rat Cytokine/Chemokine Array 27-Plex (BioPlex 200 Cytokine Array Assay Kit Source, Millipore Milliplex; Calgary, AB, Canada).
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The key features of SARS-CoV-2 leader and NSP1 required for viral escape of NSP1-mediated repressionbioRxiv - Molecular Biology 2021Quote: ... The membrane was probed with the following primary antibodies: mouse anti-flag antibody 1:2000 (F1804 Sigma), mouse anti-beta-actin 1:5000 (A2228 Sigma).
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bioRxiv - Physiology 2022Quote: ... Membranes were blocked for 1 hour at room temperature in 5% milk or 5% BSA (Millipore Sigma) in 1X Tris-buffered saline + 0.1% Tween 20 (TBST) ...
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bioRxiv - Molecular Biology 2020Quote: ... The membranes were probed with a secondary antibody: either anti-rabbit-HRP (1:5,000; Merck Millipore AP132P), anti-mouse-HRP (1:5,000 ...
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bioRxiv - Developmental Biology 2019Quote: ... followed by a second washing step using PBS membrane and coating with 1% bovine Gelatin (Sigma #G9391) for 1 hour at 37°C ...
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bioRxiv - Plant Biology 2019Quote: ... Pik-1 and AVR-Pik effectors were detected probing the membrane with anti-FLAG M2 antibody (SIGMA) and anti c-Myc monoclonal antibody (Santa Cruz) ...
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bioRxiv - Biochemistry 2020Quote: ... the membrane was blocked for 1 hour (PBS-T [Sigma, US] containing 5% skim milk powder [Sigma]) and incubated in 1:10,000 rabbit-anti-G ...
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bioRxiv - Neuroscience 2020Quote: ... separated on a SDS-PAGE gel and then electrotransfered (100V, 1 hour) on a PVDF membrane (Millipore). After blocking for 1 hour in Tris-buffered saline with 0.1% Tween-20 (TBS-T ...
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bioRxiv - Plant Biology 2020Quote: ... Pik-1 and AVR-Pik effectors were detected probing the membrane with anti-FLAG M2 antibody (SIGMA) and anti-c-Myc monoclonal antibody (Santa Cruz) ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were labeled in 5% BSA/TBST with either anti-HA peroxidase conjugated antibodies (Sigma, 1:200) or rabbit TgALD1 antibody (1:200 ...
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bioRxiv - Synthetic Biology 2021Quote: ... the membrane was incubated with secondary antibody (1:10000) anti-Goat IgG secondary antibody (A0545, Sigma Aldrich) in the buffer (10ml 1x TBST ...
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bioRxiv - Cancer Biology 2021Quote: ... Western blot was performed using PVDF membranes incubated with anti-vinculin (Sigma-Aldrich; V9131, 1:10,000 dilution) antibody as cytoplasmic loading control ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were blocked for 1 h with Tris-buffered saline (TBS) containing 0.1 % Tween 20 (Sigma Aldrich) and 5 % dry milk and further incubated for 1 h with primary antibodies diluted in TBS ...
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Construction of a constitutively active type III secretion system for heterologous protein secretionbioRxiv - Synthetic Biology 2022Quote: ... The membrane was probed with rabbit anti-GroEL according to manufacturer’s instructions (Sigma Aldrich, 1:10,000 dilution). Membranes were subsequently probed with goat anti-rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2022Quote: ... Western blotting was performed as described above and membranes were probed with anti-Cx43 (1:1000, Sigma) and anti-Cx43(1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... the membrane was incubated for 1 min in Ponceau S solution (Sigma-Aldrich, cat no. P7170-1L) and imaged ...
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bioRxiv - Neuroscience 2020Quote: ... the membranes were incubated with horseradish peroxidase (HRP)-conjugated anti-rabbit IgG (rabbit, A9169, 1:10000, Sigma) or anti-mouse IgG (mouse ...
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bioRxiv - Microbiology 2019Quote: ... Membranes were washed and incubated with polyclonal antibodies specific for alpha-toxin (1:5,000 dilution; Sigma-Aldrich) for 2 h at room temperature ...
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bioRxiv - Genetics 2021Quote: ... Membranes were soaked in a 1:1000 dilution of primary antibody (mouse anti-FLAG, Sigma Cat #F1804) overnight at 4 °C with rocking ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was diluted 1:4 in lysis buffer and filtered through a 0.22 μm membrane (Millipore). MS3_01370 was purified by IMAC by loading onto a prepacked 1 ml His-Trap HP column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... transferred to a PVDF membrane and blocked for at least 1h in 1% Casein blocking buffer (Sigma). Membranes were incubated overnight at 4°C with the following primary antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... tissue was incubated at 4°C in blocking solution with BODIPY membrane dye (Sigma, D3821, 1:1000) for 24 hours ...
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bioRxiv - Neuroscience 2021Quote: ... transferred to a PVDF membrane and blocked for 1 hour in 5% bovine serum albumin (Sigma, A7906) in TBS+0.01% Tween20 ...
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bioRxiv - Plant Biology 2019Quote: ... 1 mL of the solution was filtered through a 0.45 μm nylon membrane filter (Merck Millipore, USA).
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bioRxiv - Biochemistry 2021Quote: ... The membrane was incubated with a mono ADP-ribose binding reagent (MABE 1076, Millipore Sigma, 1:2500) and next treated with a secondary antibody (goat anti-rabbit conjugated to HRP ...
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bioRxiv - Microbiology 2021Quote: ... The membranes were incubated with 1:5,000 monoclonal ANTI-FLAG M2 antibody produced in mouse (Sigma-Aldrich) and Goat Anti-Mouse IgG (H+L)∼HRP Conjugate (BioRad) ...
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bioRxiv - Cell Biology 2021Quote: Membrane potential was measured with 1 μM tetramethylrhodamine (TMRE, excitation: 540 nm, emission: 595 nm; Sigma Aldrich) as described before (6) ...
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bioRxiv - Genetics 2022Quote: ... Membrane pieces were incubated in antibody solution {1xTBS with 1% milk and 0.2% Tween 20 (Sigma P7949)} with primary antibodies against GAPDH and CRMP2 (Supplementary Table 3 ...
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bioRxiv - Developmental Biology 2024Quote: ... Membranes were blocked at RT for 1 hour with 5 % milk diluted in PBS (Sigma-Aldrich, P4417) containing 0.1% Tween-20 (PBS-T) ...