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Citations for Millipore Sigma :
2901 - 2950 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-amyloid precursor protein (APP, C-terminal, 1:1000, Sigma, #A8717), rat anti-green fluorescent protein (GFP ...
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bioRxiv - Neuroscience 2024Quote: ... Mouse Anti-Glial Fibrillary Acidic Protein (1:100, Millipore, Cat# MAB360, RRID:AB_11212597), Mouse Anti-NeuN (1:1000 ...
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bioRxiv - Biochemistry 2023Quote: ... protease incubation (Pepsin Porcine, 1:50, enzyme to protein ratio, Sigma-Aldrich) was performed for 4 h at 37 °C in 40 mM HCl in a total volume of 2 ml (pH 2) ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were digested overnight at 37C with 1:50 trypsin (Sigma-Aldrich) and 1:75 and lysyl endopeptidase (Wako) ...
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bioRxiv - Cell Biology 2023Quote: ... and 4 g digitonin:1 g mitochondrial protein (Sigma-Aldrich, #D141-100MG). Mitochondria were incubated on ice for 20 minutes and then centrifuged at 20,000 x g for 10 minutes at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... protein expression was induced with 1 μg/mL doxycycline hyclate (Sigma Aldrich), and the culture was placed in a shaking incubator maintaining 37°C and a 5% CO2 atmosphere ...
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bioRxiv - Bioengineering 2023Quote: ... mouse monoclonal anti-glial fibrillary acidic protein (1:1000, Millipore Sigma, #MAB3402), rat monoclonal anti-CD45 (1:200 ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were precipitated with 1:4 trichloroacetic acid solution (TCA, Sigma Aldrich) and washed with acetone ...
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bioRxiv - Neuroscience 2023Quote: ... mouse anti-glial fibrillary acidic protein (GFAP, 1:1000, Millipore Sigma, USA), anti-NeuN (1:500 ...
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Molecular basis of selective cytokine signaling inhibition by antibodies targeting a shared receptorbioRxiv - Immunology 2021Quote: ... The plates were washed using ELISA washing solution followed by addition of substrate (4-Nitrophenyl phosphate disodium salt hexahydrate, Sigma-Aldrich, 1 mg/ml), 150 µl/well ...
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bioRxiv - Microbiology 2020Quote: ... lysates were diluted 1:1 in RIPA buffer and precleared with protein A agarose beads (Millipore-Sigma) for 60 min at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... lysates were diluted 1:1 in RIPA buffer and precleared with protein A agarose beads (Millipore-Sigma) for 60 min at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... The membrane was hybridized with a digoxigenin-labeled DNA probe generated with a PCR DIG Probe Synthesis Kit (Sigma). Hybridization with a 5’ probe produced an 8.1 Kb band from the WT and a 5.1 Kb band from the targeted locus ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was washed with 0.1% TBST three times for 15 minutes each and developed using ECL kit (Millipore). The blots were imaged on an Amersham Imager 600 and band intensity was quantified using Image J.
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bioRxiv - Immunology 2022Quote: ... cell corpses were stained with the PKH26 Red Fluorescent Cell Linker Kit for General Cell Membrane Labelling (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The electroporated mixture was transferred into a 1.5 mL tube to which is added PKH26 dye (0.4 µL) (PKH26 Red Fluorescent Cell Linker Kit for General Cell Membrane Labeling, Sigma) mixed with 100 µL diluent C ...
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bioRxiv - Cell Biology 2020Quote: ... the membrane was incubated with the primary antibody (rabbit anti-Sarm1, 1:500, ANASPEC 55381; Mouse anti-β-Tubulin, 1:2000, Sigma T5168) at 4°C overnight ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were incubated with anti-TSP1 (1:500, NeoMarkers catalog#MS-1066-P1) or anti-βactin (1:50000, Sigma-Aldrich catalog#A3854). Appropriate peroxidase-conjugated secondary antibodies (1:10000 ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were probed with anti-mouse-AID (1/25, eBioscience, 14-5959-82) and anti-mouse-tubulin (1/5000, Sigma-Aldrich, T9026). Then ...
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bioRxiv - Biophysics 2023Quote: ... We washed the membrane 3 times with TBST before incubating with a 1:1 ratio of horseradish peroxidase substrate and enzyme mixture (Millipore, Burlington, Massachusetts). We imaged using a Biorad Chemidoc camera set to detect chemiluminescence ...
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bioRxiv - Cell Biology 2024Quote: ... filtered through a 0.45 μm membrane and diluted 1:1 with complete medium containing 10 ug/ml polybrene (Millipore, TR-1003-G). Target cells (hTERT-RPE1 Plk1flox/Δ ...
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bioRxiv - Developmental Biology 2019Quote: Single cell suspensions of wild type AGM and YS at E9.5 and E11.5 were stained with rat anti-mouse CD144-efluor660 1:200 (eBioscience #50-1441-82, clone eBIOBV13) and 7AAD (Sigma, #A9400). Cells were analyzed and sorted on FACSAria (BD) ...
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bioRxiv - Neuroscience 2021Quote: ... 1:500 dilution of Rat polyclonal anti-GnRH antibody (Skrapits et al., 2015) and 1:250 dilution Guinea Pig polycloncal anti-NeuN (EMD Millipore). After the brain slices were washed 3x with 1XPBS they were incubated with the following secondary antibodies in blocking buffer for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated for 1 hr in blocking solution (PBS with 0.5% Triton X-100 and 5% goat serum) at RT followed by rat monoclonal anti-SST primary antibody (1:100; MAB354, Sigma-Aldrich) for at least 12 hr at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Detection was carried out by incubating with a 1:4000 dilution of a rabbit anti-rat antibody conjugated to peroxidase (A5795, Sigma-Aldrich). The membranes were then developed with the ECL Plus™ Western blotting detection system (PerkinElmer Life Sciences ...
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bioRxiv - Cancer Biology 2021Quote: ... Plates were coated with collagen type I at diluted 1:100 ratio with PBS (solution from rat tail) (Sigma-Aldrich, # C3867) for 1 h at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... the samples were incubated in 1:30 rat anti-DN-cadherin (DN-EX #8, Developmental Studies Hybridoma Bank) and 1:500 rabbit anti-GABA (A2052, Sigma-Aldrich) or 1:200 rabbit anti-Lucifer yellow (A5750 ...
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bioRxiv - Cell Biology 2023Quote: ... and of PTMs (tyrosinated tubulin: YL ½ antibody, ab6160; rat monoclonal, Abcam; acetylated tubulin: T6793 antibody, clone 6-11B-1, mouse, Sigma-Aldrich; polyglutamylated tubulin ...
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bioRxiv - Cell Biology 2023Quote: ... each coverslip was washed 3 times in PBS then placed on top of 50 µL drops of the secondary antibody mixture of goat anti-rat TRITC (1:100; Millipore Sigma) and goat anti-mouse FITC (1:100 ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked at room temperature for 1 hour in 1% casein/PBS and probed overnight at 4°C with a 1:1000 dilution of Roche monoclonal 3F10 rat anti-HA-tag antibody (Sigma 11867423001) and 1:1000 dilution of rabbit anti-P ...
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bioRxiv - Neuroscience 2023Quote: ... for 30 min at room temperature and then incubated overnight at 4°C with anti-HA (rat, 1:1000 dilution, Sigma-Aldrich) antibody in TBS containing 10% goat serum ...
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bioRxiv - Neuroscience 2024Quote: ... A rat anti-D1DR monoclonal antibody was used for D1DR-receptor staining (1:500; Sigma-Aldrich, St Louis, MO; Catalog #D2944). To enhance the GFP signals ...
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bioRxiv - Neuroscience 2021Quote: ... the integrity of the bEnd.3 cell monolayer was assessed by using trans endothelial electrical resistance in an Epithelial Volt/Ohm meter equipped with STX2 electrodes (EMD Millipore Corporation). Uptake of MEs labeled with FM 4-64 and RNA cargos labeled with ExoGlow-RNATM was quantified by using a microplate fluorescence reader (BioTek Instruments ...
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bioRxiv - Cell Biology 2019Quote: ... was used to analyze the morphology of epithelial tissue collected after surgery which were fixed with 3.7% glutaraldehyde (Sigma Cat No-G5882) in PBS for 15 min in aluminum-coated coverslip ...
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bioRxiv - Microbiology 2020Quote: ... 0.1 ng/ml human recombinant epithelial growth factor and 50 U/ml penicillin and 50 U/ml streptomycin (Sigma-Aldrich, MO, USA). The VK2 cells were seeded into 24-well tissue culture plates ...
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bioRxiv - Developmental Biology 2022Quote: ... Epithelial cells in suspension were collected in mTEC Plus medium [mTEC Basic supplemented with 10 μg/ml insulin (Sigma-Aldrich, Cat #I1882), 5 μg/ml transferrin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... followed by transfer to a PVDF membrane (Immobilon-PSQ PVDF membrane, Millipore-Sigma; Burlington, MA). Immunoblotting was performed as described earlier [41] ...
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bioRxiv - Cell Biology 2020Quote: ... followed by transfer to a PVDF membrane (Immobilon-PSQ PVDF membrane, Millipore-Sigma; Burlington, MA). Immunoblotting was performed as described earlier [41] ...
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bioRxiv - Developmental Biology 2022Quote: ... prior to transfer to a PDVF membrane (Immobilon-P Membrane, Merck Millipore Ltd., Burlington, MA). The membrane was then incubated in a 5% milk tris-buffered saline with 0.1% tween-20 (TBS-T ...
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bioRxiv - Neuroscience 2022Quote: ... and transferred to nitrocellulose membrane (AmershamTM Protran® Western Blotting Membrane, Nitrocellulose, GE10600002, Sigma Aldrich) using Bio-Rad transfer apparatus ...
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bioRxiv - Neuroscience 2023Quote: ... the gel was transferred to a PVDF membrane (IPVH00010 Immobilon-P transfer membrane, Sigma-Aldrich), using the Mini Trans-Blot Cell system (BIORAD) ...
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bioRxiv - Cell Biology 2024Quote: ... and transferred in semi-dry conditions onto PVDF membrane (Immobilon-P membrane 0.45 μm, Millipore). A primary anti-GFP (#11814460001 Roche ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... the rats in SCI group were received the process of SCI and administrated with normal saline and the rats in SCI+Ex-4 group were administrated intraperitoneally (i.p.) with Ex-4 (10 µg/rat) (Sigma-Aldrich, St. Louis, MO, USA) in normal saline after SCI and dosing interval was 24h for three days ...
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bioRxiv - Immunology 2021Quote: ... ELISA plates were coated with the capturing reagent in PBS [OVA (Sigma, A5503) for antigen ELISAs ...
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bioRxiv - Microbiology 2021Quote: ... 5′5′-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA (Sigma-Aldrich, Taufkirchen, Germany) was added until a colour change was observed ...
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bioRxiv - Neuroscience 2021Quote: Alongside the ELISA a Bicinchoninic acid assay (BCA) assay (#71285-3, Novagen, Millipore) was performed according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: Alongside the ELISA a Bicinchoninic acid assay (BCA) assay (#71285-3, Novagen, Millipore) was performed according to manufacturer’s protocol ...
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bioRxiv - Pathology 2019Quote: ... BR) and insulin Elisa assay (cat#EZRMI-13K, Merck Millipore, Billerica, MA, EUA) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Overnight fasting serum levels were quantified by ELISA for insulin (#EZRMI-13K; Millipore) and leptin (#90030 ...
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bioRxiv - Genetics 2020Quote: ... Cell lines were tested for mycoplasma contamination using the Mycoplasma PCR ELISA (Sigma).