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Citations for Millipore Sigma :
2701 - 2750 of 10000+ citations for AS 1517499 CAS 919486 40 1 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... cells following subjected treatments were collected and lysed in NP40 buffer (50mM Tris-HCl pH 8, 150mM NaCl, 5mM EDTA, 0.5% NP-40, 1x EDTA-free protease inhibitor (Sigma-Aldrich), 1x phosphatase inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... The digested glomeruli were washed with 750 ⎧l 1X PBS + 0.04% BSA and filtered using a 40 ⎧m Flowmi Tip Strainer (Sigma BAH136800040). 1.25 ml 1X PBS + 0.04% BSA was added and the suspension was centrifuged at 500 xg for 8 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... 150 mM NaCl, 2 mM EGTA, 2 mM MgCl2, 0.5% NP-40, 1mM DTT, 1U/ml Benzonase from Novagen/EMD Millipore, Temecula ...
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bioRxiv - Genetics 2021Quote: ... Cleared lysates were adjusted to match the composition of the immunoprecipitation (IP) buffer (10 mM Tris-HCl, pH 8.0, 150 mM NaCl, 0.1% NP-40 alternative (EMD-Millipore product 492018). The adjusted lysates were combined with anti-VSV-G agarose beads (Sigma ...
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bioRxiv - Genetics 2020Quote: ... Crosslinked cells were resuspended in a pre-chilled lysis buffer (10 mM Tris HCl, 10 mM NaCl, 0.2% NP-40) with fresh protease inhibitors (Millipore Sigma). The lysate was then digested with the primary restriction enzyme ...
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bioRxiv - Genomics 2020Quote: ... The bottom oil layer was removed and replaced with FC-40 fluorinated oil (Sigma-Aldrich, catalog no. 51142-49-5) with 5% (w/w ...
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bioRxiv - Neuroscience 2021Quote: ... Behavioral responses were captured at 20 frames/sec using a µEye camera (IDS Imaging) fitted with a 40 mm macro lens (EX DG Macro, Sigma). Each trial contained a 120 sec baseline period followed by ∼15 sec vibratory stimulation and a 210 sec recovery period ...
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bioRxiv - Molecular Biology 2021Quote: ... The 40S- and 60S-subunit fractions were mixed and concentrated using Amicon Ultra filter units (MWCO 50 kDa, Merck Millipore). The concentrated mixture was loaded onto a 10–50% (w/v ...
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bioRxiv - Molecular Biology 2021Quote: ... as per manufacturer’s instructions and dissolved in 20-40 μl of RNase/DNase free water (Merck Millipore, Billerica, MA, USA). Quantity ...
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bioRxiv - Molecular Biology 2021Quote: Pellets of cells expressing SFB- or BioID2-tagged viral genes were subjected to lysis with NETN buffer (100mM NaCl, 1mM EDTA, 20mM Tris-HCl, and 0.5% Nonidet P-40) with protease inhibitor cocktail (Sigma-Aldrich) at 4 °C for 20 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... Beads were then washed 4x with Buffer A containing only 0.1% NP-40 and bound protein was eluted by 3 sequential incubations in PBS containing 0.1% triton x-100 (Sigma-Aldrich) and 0.5mg/ml of 3x FLAG peptide (Sigma-Aldrich ...
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bioRxiv - Genomics 2020Quote: ... The bottom oil was then removed and replaced with FC-40 fluorinated oil (Sigma-Aldrich, catalog no. 51142-49-5) with 5% (w/w ...
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bioRxiv - Microbiology 2020Quote: ... muffled 40 mL glass vials with screw caps lined with a PTFE septum (Supelco, Sigma-Aldrich Chemie GmbH, Buchs, Switzerland). Incubation (30°C ...
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bioRxiv - Physiology 2020Quote: ... followed by antigen retrieval in citrate buffer (10 mM, pH=6.0) for 40 min in 90□ and blocking in 2% BSA (A2153, Sigma Aldrich) with 0,3 M glycine (G8898 ...
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bioRxiv - Physiology 2020Quote: ... ob-Acsl1H-/- and lean and obese Acsl1flox/flox littermate control mice were injected intraperitoneally with tamoxifen (3 mg/40 g of body weight) (Sigma) dissolved in corn oil (20 mg/ml ...
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bioRxiv - Systems Biology 2020Quote: ... Seq-Well arrays were sealed in a dry 37°C oven for 40 minutes before being submerged in a lysis buffer containing guanidium thiocyanate (Sigma), 1mM EDTA ...
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bioRxiv - Bioengineering 2020Quote: ... the cells/aggregates were completely covered in Oil Red O staining solution (stock solution: 0.5 g/100 ml isopropanol, staining solution: 60 % stock + 40 % demineralized water; O0625; Sigma, USA) followed by subsequent washing steps with 60 % isopropanol and water ...
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bioRxiv - Neuroscience 2021Quote: ... 4% in PBS for 15 min at RT] and subjected to staining with filipin (40 μg/mL in PBS for 2 h at RT; from 250-fold ethanolic stock solution; F9765, Sigma). Fluorescence images of cultured cells were acquired using an inverted microscope (Axiovert 135TV ...
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bioRxiv - Neuroscience 2020Quote: ... 6-month-old littermate matched were injected subcutaneously with vehicle [40% (w/v) (2-hydroxypropyl)-β-cyclodextrin (Sigma– Aldrich #332607)] or MLi-2 dissolved in vehicle at a 30 mg/kg final dose ...
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bioRxiv - Neuroscience 2020Quote: 4% hydrogel monomer solution (HMS) was prepared for perfusion by mixing 40% (w/v) acrylamide (4% final concentration; V900845, Sigma), 2% (w/v ...
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bioRxiv - Cell Biology 2021Quote: ... and the cavity filled with freshly prepared 90-100μl GLOX-MEA buffer (0.5 mg/ml glucose oxidase (Sigma-Aldrich, Cat# G7141, 40 μg/ml catalase (Sigma-Aldrich) 10% w/v glucose (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... 420 µL clarified lysate was retrieved and added to 40 µL packed volume of anti-FLAG M2 agarose slurry (Sigma), Pierce anti-HA agarose slurry (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... Cleaved His-tagged Mistics and remaining TEV protease were removed via a second Ni-NTA purification after buffer exchange to Tris buffer containing 40 mM imidazole in an Amicon centrifugal filter (Merck Millipore) with adequate cutoff ...
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bioRxiv - Microbiology 2020Quote: ... The remaining crude lydate was cleared by centrifugation and incubated with 40 μl prepared Anti-FLAG M2 beads (Sigma-Aldridge) with rotation at 4°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... OD600nm adjusted to 0.1 (measured in 100 µL volume in a clear flat bottom 96 well plate) and supplemented with 1.6 mg/ml Red#40 dye (Sigma, Israel). Fifty µL of this culture were pipetted to rows A-G of the plate containing the amoeba ...
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bioRxiv - Microbiology 2021Quote: ... was used only in the delta variant hamster study and it was formulated as 40 mg/ml in a vehicle containing 60% PEG400 (Sigma) in sterile distilled water ...
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bioRxiv - Plant Biology 2021Quote: ... Cleaved His-tagged GB1 and remaining TEV protease were removed via a second Ni-NTA purification after buffer exchange to HEPES buffer containing 40 mM imidazole using an Amicon Ultra-10K centrifugal filter (Merck Millipore). The tag-free protein was subjected to SEC using a Superdex S75 Increase 10/300 column equilibrated in HEPES buffer without imidazole and a pH of 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... parasites at 32 hpi and 38 hpi were cultured at 37 °C or 40 °C for 6 h in the presence or absence of 20 μM Wortmannin (Selleckchem, Houston, TX) or 40 μM LY294002 (Sigma). The cells were subsequently washed and resuspended in complete medium (1% hematocrit) ...
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bioRxiv - Immunology 2022Quote: ... were isolated as previously described (40) using ethylenediaminetetraacetic acid (EDTA) containing calcium-free media and collagenase VIII (Sigma-Aldrich, C2139), respectively ...
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bioRxiv - Physiology 2022Quote: WT female C57BL6 mice were anesthetized with Pentobarbital then transcardially perfused with 20 ml PBS followed by 40 ml 10% Formalin/PBS (Sigma). Brains were removed and incubated in 15% sucrose/10% Formalin overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... diluted with 600 µL of dilution buffer (lysis buffer without NP-40) and incubated with 25 µL of pre-washed Anti-FLAG M2 Magnetic Beads (Sigma) on a rotor overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: Human glioblastoma cancer stem cells Gli4 and Gli7 were cultured in T75 tissue culture flasks precoated with 40 μg/cm2 of poly-2-hydroxyethyl methacrylate (poly-HEME, Sigma) to avoid cell adhesion ...
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bioRxiv - Neuroscience 2021Quote: ... Sucrose stock solutions (10, 16, 22, 28, 34, 40, 46, 52, 58, 64, 70 and 90%) (Sigma-Aldrich, MO, USA) were prepared in 1x PBS (PAN Biotech ...
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bioRxiv - Microbiology 2020Quote: ... 5 μl of an overnight culture of OD600 = 5 suspended in water was spotted onto LB without NaCl agar plates containing the dye Congo red (40 μg/ml, Sigma) and Coomassie Brilliant Blue G-250 (20 μg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... flow channels of the Drop-seq chip were subjected to a hydrophobicity treatment using 1H,1H,2H,2H-Perfluorodecyltrichlorosilane (in 2% v/v in FC-40 oil; Alfa Aeser/Sigma). After 5 min of treatment ...
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bioRxiv - Neuroscience 2021Quote: ... one striatal site per animal was pressure-injected with 40 nl of wheat germ agglutinin conjugated to horseradish peroxidase (WGA-HRP; Sigma) (Table 1) ...
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bioRxiv - Microbiology 2021Quote: ... Lysates were first precleared by incubation with 40 µL of protein A-sepharose beads CL-4B (Sigma, #GE17-0963-03) for 1 h at 4°C and then incubated with mouse mAb anti-HA (clone 16B12 ...
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bioRxiv - Biochemistry 2020Quote: ... Remaining protease activity was then detected using a final concentration of 40 μM of Z-FR-AMC (Sigma-Aldrich, C9521). Fluorescent activity was recorded as outlined above ...
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bioRxiv - Bioengineering 2021Quote: ... the oil phase consisted of 2.2% Ionic Krytox 157 FS-H in HFE7500 and the aqueous phase consisted of 40 μM DFHBI (Sigma-Aldrich) with or without 50 nM Broccoli aptamer in binding buffer (40 mM Tris HCl ...
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bioRxiv - Systems Biology 2021Quote: ... 10 mL of lysis buffer (IBA LifeSciences Buffer W supplemented with 0.5% NP-40 substitute and one tablet of protease inhibitor (cOmplete™ EDTA-free Protease Inhibitor Cocktail, Sigma Millipore) was added to the thawed pellet ...
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bioRxiv - Neuroscience 2022Quote: ... The suspension containing the SGNs was filtered through a 40-μm cell filter and planted on glass coverslips pretreated with 0.5 mg/ml poly D-lysine (Sigma-Aldrich) and 1 mg/ml laminin (Sigma-Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... This cell suspension is passed through successive filters in this order: metal mesh sieve filter (size 40 then 60, Sigma), and nylon cell strainer (100 μm then 40 μm ...
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bioRxiv - Microbiology 2022Quote: ... cells were analyzed on Amnis Mark II ImageStream (405 nm, 488 nm, 561 nm, and 642 nm excitation lasers, 40× magnification; EMD-Millipore) and data analysis was performed using IDEAS software (Amnis).
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bioRxiv - Cell Biology 2022Quote: ... and epidermal growth factor (EGF, #E9644, stock concentration: 40 μg/mL in 10 mM acetic acid + 0.1% BSA) was from Sigma Aldrich, St ...
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bioRxiv - Neuroscience 2022Quote: ... PEG 3350 40% v/v) with the DNA mix to be recombined and 50 μg of salmon sperm denaturated DNA (31149, Sigma) and then cooled for at least 5 min on ice ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were sliced 40 µm thick on a self-cooling microtome and stored in a 0.02% sodium azide (Millipore Sigma) PBS solution ...
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bioRxiv - Neuroscience 2020Quote: ... To retrogradely label callosally projecting neurons for areal identification in the left hemisphere, 30-40 pressure injections (Picospritzer, Parker-Hannafin) of bisbenzimide (5% in water, 20 nl each; Sigma) were made into the right hemisphere (occipital ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins (40 μg) from cell homogenates were separated on a 10% SDS-polyacrylamide gel and electrotransfered to polyvinylidene fluoride membranes (Millipore). Each membrane was washed with Tris-buffered saline Tween-20 ...
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bioRxiv - Cell Biology 2020Quote: ... Conditioned medium was concentrated ~40 times by Amicon Ultra-15 Centrifugal Filter Units with 30 kDa cutoff filter (Millipore, UFC903024), and then added to primary neuron cultures at 10 days in vitro (plated at 1.5 × 105 cells per well in a poly-D-lysine-coated 24-well plate) ...
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bioRxiv - Plant Biology 2021Quote: ... 2.5 to 40 µg of total protein extracts were separated on SDS-PAGE gels and blotted onto PVDF Immobilon-P membrane (Millipore). AGO1 protein was detected using the anti-AGO1 antibody (rabbit polyclonal ...