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Citations for Millipore Sigma :
2501 - 2550 of 10000+ citations for AS 1517499 CAS 919486 40 1 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... All 40 brains were extracted from the skull in ice-cold Hanks’ balanced salt solution (HBSS, Sigma, UK). Hindbrains were cut using a vibroslicer (Campden Instruments ...
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bioRxiv - Immunology 2024Quote: ... a 10ml clearing solution was prepared using 5.5ml 40% (v/v) N-methylacetamine (diluted with PBS; Sigma-Aldrich) and 10g Histodenz (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... Following electrophoresis, proteins were transferred overnight (40 V, 4°C) to a PVDF membrane (Millipore Sigma-Aldrich, #IPVH00010) in Tris-glycine buffer (25 mM Tris ...
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bioRxiv - Bioengineering 2024Quote: ... Afterwards the cells were washed twice with washing buffer consisting of 0.05% (v/v) NP-40 (SIGMA-ALDRICH) and 5mM EDTA (SIGMA-ALDRICH ...
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bioRxiv - Cell Biology 2024Quote: ... 2 months animals were treated with Lipopolysaccharides from Escherichia coli O55:B5 (40 mg/kg) (Sigma-Aldrich L2880). To induce severe tissue damage ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then stained with 40 mM Alizarin red S (pH 4.1) (Sigma Aldrich, St. Louis, MO, USA) for 20 minutes and then washed with fresh deionized water 5 times for 5 minutes ...
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bioRxiv - Biophysics 2024Quote: ... cells were cultured to 70%-80% confluency and treated with 40-100 units of SLO (Sigma-Aldrich, S5265) for five minutes ...
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bioRxiv - Bioengineering 2020Quote: ... and 800 mg L-1 humic acids (CAS No. 1415-93-6) were prepared by adding in ultrapure water (Sigma Aldrich, UK).
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bioRxiv - Neuroscience 2022Quote: ... Slices were then incubated in primary antibody for 48 hours at 4°C (ChAT (1:200): goat anti-ChAT (Abcam Cat. #ab144P, Millipore, Temecula, CA), PV (1:2000) ...
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bioRxiv - Neuroscience 2020Quote: ... fixed overnight (ON) in paraformaldehyde 4% and stored at -20 °C in cryoprotectant (150 g of sucrose (Sigma - CAS 57-50-1) and 150 mL of ethylene glycol (Sigma - CAS 107-21-1 ...
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bioRxiv - Microbiology 2021Quote: ... The resulting hybridoma cells were plated onto 96-well plates and cultured in HAT selection medium (Hybridoma-SFM [Life Technologies, Grand Island, CA, USA]; 10% FBS; 1 ng/mL mouse IL-6; 100 μM hypoxanthine [Sigma-Aldrich, St ...
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bioRxiv - Synthetic Biology 2020Quote: ... and blocked for 1h in 3% BSA (Fisher bioreagents CAS 9048-46-8) and 0.1% Triton X-100 (Sigma 9002-93-1). The cells were then incubated in primary antibody overnight ...
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bioRxiv - Cell Biology 2020Quote: ... Then the cells were washed with PBS 3 times and incubated with cyanine 3 (Cy3)-conjugated donkey anti-goat IgG secondary antibody (1:500, Millipore, Temecula, CA) at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were incubated for 48 h at 4°C with primary mouse anti-TH antibody (anti-mouse-TH; MAB-318, 1:1000; Millipore, Temecula, CA). The sections were then incubated for 1 h with the appropriate secondary antibody after which sections were washed with PBS and incubated with the required detection kits for development ...
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bioRxiv - Plant Biology 2024Quote: ... The pistils of emasculated flowers were immersed uniformly in 100 µM GA3 or 50 µM picloram (Sigma-Aldrich, CAS-1918-02-1) solution that also contained 0.01% (v/v ...
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bioRxiv - Bioengineering 2024Quote: ... We also prepared an EDTA/Ca buffer containing 50 mM EDTA and 50 mM CaCl2 by diluting 1 M CaCl2 (Sigma, 21115-25L) and 0.5 M EDTA (pH 8.0 ...
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bioRxiv - Genetics 2022Quote: Fipronil (CAS# 120068-37-3) and Spinosad (CAS# 168316-95-8) were both obtained as analytical standards from Sigma. Insecticide bioassays were performed as described previously [20] ...
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bioRxiv - Microbiology 2021Quote: ... ammonium chloride (>99.5%, CAS number: 12125–02–9), ammonium formate (>97%, CAS number: 540-69-2) were acquired from Sigma–Aldrich ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... taurine (CAS 107-35-7) and ascorbic acid (CAS 50-81-7) were obtained from Sigma-Aldrich (Taufkirchen, Germany). 1M sucrose solutions (Südzucker AG ...
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bioRxiv - Cell Biology 2020Quote: ... and the pellets were directly lysed in 750 µl of Polysome Lysis Buffer (10mM HEPES pH 7.4, 150mM KCl, 5mM MgCl2, 0.5% NP-40, 0.5mM DTT (Sigma-Aldrich, D0632), 50mM iodoacetamide ...
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bioRxiv - Cell Biology 2020Quote: ... Concanamycin A (Vacuolar type ATPase inhibitor; used at 10 nM) and 3,4-Dichloroisocoumarin (granzyme inhibitor; used at 40 μM) were purchased from Sigma, Santa Cruz Biotechnology and Cayman Chemical ...
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bioRxiv - Cell Biology 2020Quote: ... 150 mM NaCl, 2 mM EGTA, 2 mM MgCl2, 0.5% NP-40, 1mM DTT, 1U/ml Benzonase from Novagen/EMD Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... The cell pellet was resuspended in 100 μl of Proteinase K extraction buffer [40 mM Tris-HCl (pH 8.0) (Sigma), 1% Tween-20 (Sigma) ...
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bioRxiv - Developmental Biology 2021Quote: Whole embryonic mouse brains were harvested at E15.5 and lysed in NP-40 lysis buffer with protease inhibitor cocktail (Sigma). Whole brain lysates from one embryo were added to either 6μg GST or 6μg GST-MLLT11 bead slurries and nutated overnight at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... lysed them in NP40 lysis buffer (50mMTris-HCl, 150mMNaCl, 0.1% NP-40, 5mM EDTA, 10% glycerol) with protease inhibitors cocktail (Sigma), and sonicated using a Bioruptor (Diagenode) ...
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bioRxiv - Molecular Biology 2021Quote: ... then they were resuspended in cell lysis buffer (5 mM PIPES pH 8.0, 85 mM KCl, 0.5% NP-40; Sigma-Aldrich) complemented with 1xPIC (Roche ...
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bioRxiv - Molecular Biology 2020Quote: ... The hydrogel beads were composed of a 4xAB solution (2.6 ml 40% acrylamide in water (Sigma-Aldrich, cat #01697), 3.6 ml 40% total 19:1 acrylamide:bis-acrylamide aqueous solution (Bio-lab ...
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bioRxiv - Molecular Biology 2020Quote: ... then 10min at 4°C in the Lysis buffer 2 (50mM Tris pH8, 10mM EDTA, 0.5% NP-40, Complete protease inhibitor (Sigma)) and subsequently sonicated in 15ml conical tubes with a Bioruptor Pico (Diagenode ...
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bioRxiv - Microbiology 2022Quote: ... 900 μL of the supernatant were incubated with 40 μL of Agarose Anti-FLAG M2 gel beads (Sigma-Aldrich) and incubated overnight (ON ...
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bioRxiv - Genomics 2020Quote: ... cells were lysed on ice in 5 ml lysis buffer (10 mM Tris-HCl, pH 8, 10 mM NaCl, 0.2% Igepal NP-40 (Sigma), 1× cOmplete protease inhibitor (Roche ...
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Mapping of the autophagosomal degradome identifies IL-7Rα as key cargo in proliferating CD4+ T-cellsbioRxiv - Immunology 2021Quote: ... and added to the cells for 5 min without and for another 40 min with 10 mM H2O2 (Sigma). After washes in CB ...
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bioRxiv - Cancer Biology 2020Quote: ... ~2×108 cells were collected and lysed in a hypotonic buffer (5 mM PIPES pH 8, 85 mM KCl, 0.5% NP-40) supplemented with Complete Protease Inhibitor Cocktail (Sigma) for 20 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... This solution was overlaid on top of a gradient (17%, 25%, 40%, 60%) of Iodixanol (Millipore Sigma, cat. #D1556) and ultracentrifuged at 46,500 rpm (Beckman Coulter VTi50 ...
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bioRxiv - Plant Biology 2020Quote: ... and ground in liquid N2 in 2 volumes of extraction buffer (50 mM Tris–HCl pH 7.5, 150 mM NaCl, 10% glycerol, 0.1% Nonidet P-40 and 1x protease inhibitor cocktail; Sigma-Aldrich) and centrifuged for 12,000 g for 10 min at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... to assess protein concentration and then solubilized in 4x loading dye (250 mM Tris-HCl pH 6.8, 40% glycerol, 0.008% bromophenol blue, 8% SDS; all from Sigma-Aldrich). All samples were boiled at 65°C for 10 minutes and then equal amounts of each sample were separated using SDS-PAGE and subsequently blotted on nitrocellulose membranes using the Trans-Blot Turbo System (Bio-Rad) ...
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bioRxiv - Neuroscience 2020Quote: Mice were anesthetized (disodium salt pentobarbital, 40 mg/kg, IP) and intracardially perfused with cold PBS (Sigma-Aldrich, USA) and paraformaldehyde 4 % (Electron Microscopy Sciences ...
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bioRxiv - Cell Biology 2021Quote: ... 0.5 mM DTT, 2 µg/ml leupeptin, 40 U/ml RNAsin, 1.4 µg/ml pepstatin, 0.2 mg/ml heparin [Sigma-Aldrich] ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were extracted using 1.6 ml of extraction/washing buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.1% Triton, 0.2% NP-40, 6 mM BME and 1x protease inhibitor cocktail [SIGMA, USA]) during 30 minutes at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... on coverslips previously treated 30-40 minutes with a sterile bovine gelatin solution (0.1% v/v in PBS, Sigma) and incubated for 24 hours prior to irradiation ...
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bioRxiv - Biophysics 2020Quote: The water phase was prepared by mixing two parts of acrylamide solution (40 % v/v, Sigma, St. Louis, USA) with one part of N,N’-methylenebisacrylamide solution (2 % v/v ...
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bioRxiv - Systems Biology 2022Quote: ... Cell pellets were then lysed in 0.1% NP-40 Tris-NaCl lysis supplemented with protease and phosphatase inhibitors (Sigma). Cell lysis was facilitated by three cycles of rapid freeze-thawing in liquid nitrogen ...
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bioRxiv - Biophysics 2022Quote: ... Protein was concentrated up to 40–60 mg/ml using a 100 kDa molecular weight cut-off concentrator (Millipore). The protein purity was checked by SDS-PAGE ...
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bioRxiv - Neuroscience 2022Quote: ... Alkyne agarose (25 μl per sample) was washed with milli-Q and pre-blocked with 2% PVP-40 (Sigma) in buffer 1 (100 mM Tris pH 8.5 ...
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bioRxiv - Cell Biology 2022Quote: ... cell pellets were resuspended in 500 μl 50mM Na-Citrate and treated with 0.7 μl RNAse A (20-40 mg/ml stock; Sigma) at 50°C for at least 1 day ...
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bioRxiv - Genomics 2022Quote: ... The pellet was resuspended in the same volume of Buffer I and an equivalent volume of ice-cold Buffer I supplemented with 0.2% NP-40 alternative (Sigma) was added ...
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bioRxiv - Synthetic Biology 2022Quote: ... were used in this work: The perfluorinated amine Fluorinert™ FC-40 was purchased from Sigma-Aldrich (Overijse, Belgium), while the perfluorinated ether FC-770 and the perfluorinated amine FC-3283 were obtained from Acros Organics (Geel ...
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bioRxiv - Neuroscience 2022Quote: ... First 400 µL of 100 mM pentafluorobenzyl bromide (PFB-Br, CID: 1765-40-8, Sigma-Aldrich, Catalog number: 101052), prepared in acetonitrile (Fisher Chemical ...
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bioRxiv - Biochemistry 2022Quote: ... in a stoppered quartz cuvette (Helma 110-10-40) fitted with a Suba seal silicone septum (Sigma Z279730-25ea) and an 8 mm x 3 mm pivoted spin bar (VWR 37119-6183) ...
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bioRxiv - Neuroscience 2020Quote: ... 5 min) and equally distributed in wells containing growth medium (DMEM-F12-glutamax [Gibco], 40 μg/mL insulin [Sigma] ...
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bioRxiv - Developmental Biology 2021Quote: ... Larvae were incubated for 40 minutes at 28°C in 300 µl of trypsin solution (trypsin-EDTA, Sigma T4299) containing 1 µl of Hoechst (Hoechst 33342 ...