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Citations for Millipore Sigma :
2751 - 2800 of 10000+ citations for AS 1517499 CAS 919486 40 1 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... and epidermal growth factor (EGF, #E9644, stock concentration: 40 μg/mL in 10 mM acetic acid + 0.1% BSA) was from Sigma Aldrich, St ...
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bioRxiv - Neuroscience 2022Quote: ... PEG 3350 40% v/v) with the DNA mix to be recombined and 50 μg of salmon sperm denaturated DNA (31149, Sigma) and then cooled for at least 5 min on ice ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were sliced 40 µm thick on a self-cooling microtome and stored in a 0.02% sodium azide (Millipore Sigma) PBS solution ...
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bioRxiv - Neuroscience 2020Quote: ... To retrogradely label callosally projecting neurons for areal identification in the left hemisphere, 30-40 pressure injections (Picospritzer, Parker-Hannafin) of bisbenzimide (5% in water, 20 nl each; Sigma) were made into the right hemisphere (occipital ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins (40 μg) from cell homogenates were separated on a 10% SDS-polyacrylamide gel and electrotransfered to polyvinylidene fluoride membranes (Millipore). Each membrane was washed with Tris-buffered saline Tween-20 ...
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bioRxiv - Cell Biology 2020Quote: ... Conditioned medium was concentrated ~40 times by Amicon Ultra-15 Centrifugal Filter Units with 30 kDa cutoff filter (Millipore, UFC903024), and then added to primary neuron cultures at 10 days in vitro (plated at 1.5 × 105 cells per well in a poly-D-lysine-coated 24-well plate) ...
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bioRxiv - Plant Biology 2021Quote: ... 2.5 to 40 µg of total protein extracts were separated on SDS-PAGE gels and blotted onto PVDF Immobilon-P membrane (Millipore). AGO1 protein was detected using the anti-AGO1 antibody (rabbit polyclonal ...
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bioRxiv - Immunology 2020Quote: ... pH 8.0, 5 mm EDTA, 150 mM NaCl and 0.5% NP-40, supplemented with Protease Inhibitor Cocktail, Sigma-Aldrich, Israel). Protein extracts were then precleared with protein G beads (EZview™ Red Protein G Affinity Gel ...
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bioRxiv - Microbiology 2021Quote: ... To visualize protein bands for excising and mass spectrometry processing, gels were stained with Coomassie (40% methanol, 20% acetic acid, and 0.1% Brilliant Blue R (B7920, Sigma-Aldrich)) ...
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bioRxiv - Immunology 2020Quote: ... and 3×105 PBMC per well were seeded with 40 μl assay medium in XF 96-well cell culture microplate coated with poly-D-lysine (Sigma). The plate was centrifuged at 200 x g for 1 sec with no brake ...
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bioRxiv - Physiology 2020Quote: ... of all mice were injured with 40 μl of cardiotoxin (Naja kaouthia, 0.071 mg/ml; catalog no. C9759, Sigma-Aldrich); the contralateral limb served as the uninjured control ...
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bioRxiv - Cell Biology 2021Quote: ... then centrifuged at 10,000 x g for 10 min and the supernatant fraction (800 μl) was applied to 40 μl streptavidin-agarose beads (Sigma) followed by rotation overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Digested tissues were passed through a 70-µm cell strainer and washed once with 40 ml PBS containing 5% FBS and treated with red blood cell lysing buffer (Sigma). The number of viable cells was quantified by trypan blue staining.
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bioRxiv - Developmental Biology 2021Quote: ... mRNA was first isolated from 40 dpf wild-type AB strain ovaries using TRI reagent (Cat. No. T9424; Sigma-Aldrich) and then cDNAs were synthesized with RETROScript Reverse Transcription Kit (Cat ...
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bioRxiv - Microbiology 2021Quote: ... for 40 minutes after which refractive index matching and optical clearing were performed overnight with dibenzyl ether (108014, Sigma-Aldrich).
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bioRxiv - Immunology 2021Quote: ... tissue pieces remaining after the DTT/EDTA step were chopped and incubated for 40 min at 37°C with Collagenase D (2 mg/ml; Sigma) and DNase (1 mg/ml ...
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bioRxiv - Immunology 2021Quote: ... Arrays were sealed in a dry 37°C oven for 40 min and submerged in a lysis buffer containing guanidium thiocyanate (Sigma), EDTA ...
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bioRxiv - Cell Biology 2021Quote: ... and with 10mM EDTA then incubated with Goat anti plexin-A2-APC (RαD systems) for 40 min in the dark at 25 °C for staining plexin-A2 or with Mouse anti-FLAG (Sigma) for 40 min at 4°C followed by Goat anti mouse IgG H&L (Alexa Fluor®647)(abcam ...
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bioRxiv - Biochemistry 2020Quote: P-gp was incubated with a 10x molar excess of membrane scaffold protein (MSP) and a 500x molar excess of 40% L-alpha-phosphatidylcholine (PC) from soybean (Sigma) for 1 hour at room temperature with gentle agitation to facilitate formation of nanodiscs [73] ...
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bioRxiv - Genetics 2020Quote: ... Equal amounts (Protein estimation by Bradford) of soluble fractions were incubated with 40 μl of pre-equilibrated anti-FLAG M2 affinity agarose gel (Sigma) in binding buffer (Lysis buffer + 1% Triton X-100) ...
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bioRxiv - Biochemistry 2022Quote: ... The 300 μL mixtures were adjusted to a 40% OptiPrep Density gradient medium by mixing with 200 μL 100% (w/v) OptiPrep Density gradient medium (Sigma). The mixture was transferred to a 3.2 mL ...
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bioRxiv - Cell Biology 2022Quote: ... Pellets were washed twice with HBSS and then resuspended in discontinuous 40/70% Percoll® (Sigma-Aldrich, St Louis, MO.) gradient and centrifuged at 2500 rpm for 30 min (Qui and Sheridan ...
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bioRxiv - Cell Biology 2022Quote: ... and differentiated at 4×106 cells per 40 mm dish for 7 days at 37 °C in RPMI media (Sigma) supplemented with 1 mM Sodium Pyruvate (Sigma) ...
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bioRxiv - Immunology 2022Quote: ... was used at 0.5 µg/mL in PBS-BSA/500 mM NaCl/0.1% NP-40 alternative (EMD Millipore, Billerica, MA) for 60 minutes at room temperature ...
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bioRxiv - Genetics 2022Quote: ... Equal amounts of protein (40 µg) were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred onto Immobilon-P membranes (Millipore), as previously described (Nakamori et al. ...
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bioRxiv - Neuroscience 2022Quote: Cells were fixed with 4% paraformaldehyde for 10 min and permeabilized for 40 min using 0.1% Triton X-100 (Sigma-Aldrich) in PBS ...
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bioRxiv - Neuroscience 2022Quote: Polyacrylamide substrates on glass coverslips were prepared as previously described12 from a combination of acrylamide (40% w/w; A4058, Sigma), bisacrylamide (2% ...
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bioRxiv - Physiology 2023Quote: ... male and female mice 8-10 weeks of age were injected (i.p.) with 40 mg/kg tamoxifen (Sigma-Aldrich, T5648) for five consecutive days ...
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bioRxiv - Biochemistry 2022Quote: ... Yeast pellets were resuspended in 1 ml lysis buffer1 composed of 0.1 % nonyl phenoxypolyethoxylethanol (NP-40) and Phosphate-Buffered Saline (PBS, 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4 and KH2PO4, Sigma Aldrich) supplemented with protease inhibitor (cOmplete™ Protease Inhibitor Cocktail ...
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bioRxiv - Neuroscience 2022Quote: ... Protein samples (40 μg) were separated by 4-10% SDS-PAGE and transferred to a PVDF membrane (Immobilon-P, Millipore). Membrane was stained with anti-OXPHOS (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... was purchased from Jackson ImmunoResearch and NP-40 (catalog no. T8786) and Triton X-100 (TX-100; catalog no. T8787) was purchased from Sigma-Aldrich.
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bioRxiv - Molecular Biology 2022Quote: ... the eluted protein is pooled and concentrated to ∼40 mg/mL by using a 50 kDa cutoff ultrafiltration unit (Millipore). Finally ...
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bioRxiv - Neuroscience 2022Quote: ... 3 or 7 days after CCI (alongside age-matched naïve controls) via transcardial perfusion with 40 mL 1X phosphate-buffered saline (PBS) followed by 80 mL 4% paraformaldehyde (PFA, Sigma) in PBS at a rate of 10 mL/min ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were recovered using 40 μL of 50 mM TEAB buffer followed by 50 μL of 0.5 M NaCl (Sigma-Aldrich). Peptides were desalted using C18 solid phase extraction spin columns (The Nest Group) ...
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bioRxiv - Cell Biology 2022Quote: ... The tissues were treated for 20-40 min at +4⁰C with 1,25U/ml of Dispase II (4942078001; Sigma Aldrich) in PBS and 3-4 min at room temperature with a pancreatin-trypsin mixture (2.5mg/ml pancreatin [P3292 ...
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bioRxiv - Immunology 2023Quote: ... lung sections were washed twice with PBS for 2 minutes at RT and mounted with Mowiol 40-88 (Sigma-Aldrich). Confocal microscopy images were acquired using a Zeiss LSM 880 instrument ...
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mTORC1-dependent SOCE activity regulates synaptic gene expression and muscle response to denervationbioRxiv - Physiology 2024Quote: ... loaded with 2 μM of Fura-2 AM (F1221, ThermoFischer Scientific), 0.1 % pluronic acid (Pluronic F-127, P3000MP, ThermoFischer Scientific) and 40 µM of BTS (203895, Sigma-Aldrich) in the dark at room temperature for 60 min ...
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bioRxiv - Cancer Biology 2023Quote: ... staining solution (0.3 mg/ml of 5-bromo4-chloro-3indolyl β-D-galactoside, X-Gal Fermentas R0401, 40 mM citric acid, Sigma C-0759 ...
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bioRxiv - Biochemistry 2024Quote: ... cells were harvested in NP-40 lysis buffer and mixed with anti-FLAG M2 magnetic beads (Sigma-Aldrich, Cat# F4799) for 2 hours at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... 24 hours later the media was changed and 40 µL of Pff was added to the culture along with 200 nM Bafilomycin (Sigma) or an equivalent volume of DMSO ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were spun at 30,000 g for 10 min at 4°C and the cleared lysate was incubated with 40 µl anti-FLAG agarose bead slurry (Sigma) for 30 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... into C57BL/6J mice (CD45.2+) one hour before immunization with 40 μg of ovalbumin emulsified with RIBI (Sigma Adjuvant System). CD4+ T cell populations from the spleen were analyzed 2 and 3 days after immunization.
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bioRxiv - Molecular Biology 2023Quote: ... the elutions containing ORC (typically 2-5) were pooled and incubated with 40 µl of equilibrated Anti-V5-antibody-coupled Agarose Affinity Gel (Sigma) for 30 min at 4°C with rotation ...
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bioRxiv - Neuroscience 2023Quote: ... Cell lysis was performed by dissolving the bacterial pellet in 100 mL of 40 mM Tris-hydrochloride (HCl; Sigma-Aldrich) buffer (pH 7.5 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... mice (N≥5 per group) were administered (s.c.) various doses of mifepristone (10, 20, 25, 30, 40, 60, 80, 100, 150 μg; Sigma M8046) to establish the lowest dose required to induce PTB in 100% mice (prior to day 18.5 of pregnancy ...
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bioRxiv - Cell Biology 2023Quote: ... and dounced for 100 strokes in a 40-ml Dounce homogeniser (using the small clearance pestle, or the pestle B; cat. D9188, Sigma), followed by two times of centrifugation for 5 min at 600g at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... After addition of 250 µL 4× lysis buffer (4% SDS, 40 mM tris(2-carboxyethyl)phosphine (TCEP; Sigma-Aldrich, C4706), 160 mM chloroacetamide (CAM ...
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bioRxiv - Neuroscience 2023Quote: ... Adult female SNSiDTR (n=6) and TrkBiDTR (n=19) mice were injected i.p with 40 μg/kg DTX (Sigma, D0564) with a second dose after 72 h ...
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bioRxiv - Bioengineering 2023Quote: ... in the 40% fraction and concentrated in PBS (Phosphate Buffer Saline) with 100K cut-off concentrator (Amicon Ultra15, MERCK-Millipore). Virus titers were determined using AAVpro Titration Kit Ver2 (TaKaRa).
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bioRxiv - Microbiology 2023Quote: ... Fresh HEK-293T cells were plated in 6 well plates precoated with poly-D-lysine and were infected with 3 ml of lentiviral supernatant by constant centrifugation at 2.000 rpm for 40 min in the presence of 10 µg/ml Polybrene (Sigma-Aldrich). At 72 hpi ...