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Citations for Millipore Sigma :
2951 - 3000 of 10000+ citations for AS 1517499 CAS 919486 40 1 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... All plates except Total were then washed twice with ice-cold PBS and incubated for 40 minutes at 4°C with MESNA-containing buffer (Sigma Aldrich) to remove remaining surface-associated biotin ...
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bioRxiv - Molecular Biology 2022Quote: ... 150 μl of total cell lysates were used as input and 850 µl were incubated with 40 μl anti-FLAG M2 magnetic bead (Sigma M8233) at 4°C overnight while rotating ...
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bioRxiv - Genetics 2020Quote: ... 500 μL of the crude lysate was incubated with 40 μL of 50 % slurry of α-Flag M2 beads (Sigma-Aldrich) for 2 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... The pAG-MNase digestion was halted by the addition of 50 µl 4× STOP solution (680 mM NaCl, 40 mM EDTA (Sigma, E5134), 8mM EGTA (AG Scientific ...
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bioRxiv - Microbiology 2021Quote: ... B20 biodiesel was filter sterilized into an autoclaved 1L Schott Bottle using a Steritop™ bottle top filter unit with a 40 cm2 filter area and 0.22 µm nominal pore size (Millipore Sigma) and 3 mL of this biodiesel was aseptically added to the tubes ...
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bioRxiv - Microbiology 2021Quote: ... 1 L of B20 fuel was passed through a Steritop™bottle top filter unit with a 40 cm2 filter area and 0.22 µm nominal pore size (Millipore Sigma). This filter was cut out with a sterile scalpel ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Aliquots (40 μg) of protein samples were subjected to 10% SDS-PAGE and transferred to PVDF membranes (Millipore, Bedford, MA, USA). After blocking with 5% nonfat milk (dissolved in TBST ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Larvae were collected and prepared for the assay as detailed above except that they were kept in food with 40 μM all trans-retinal (ATR, Sigma-Aldrich) and in dark for 72 hours ...
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bioRxiv - Cell Biology 2021Quote: ... mice were i.p injected for 5 consecutive days with 40 mg/kg body weight of Streptozotocin (STZ) (Sigma-Aldrich, S0130-1G) in PBS 24-28 weeks post-transplantation ...
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bioRxiv - Plant Biology 2022Quote: ... the digested tissue suspensions were filtered through 40 μm nylon mesh and washed by W5 buffer with the components of 154 mM NaCl (Sigma, S5886), 5 mM KCl (Sigma ...
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bioRxiv - Cell Biology 2021Quote: Approximate intracellular water volume of FUCCI L1210 cells was measured in the SMR by growing the cells in culture media containing 40% OptiPrep Density Gradient Medium (Sigma-Aldrich). The OptiPrep containing media has a solution density which is closer to cell’s dry density than the density of water ...
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bioRxiv - Immunology 2020Quote: ... Typhimurium wt strain were cultured at 28°C and 37°C respectively and the bacterial suspensions were prepared with phosphate buffered saline (PBS) with 2% (w/v) polyvinylpyrrolidone-40 (PVP40, Sigma-Aldrich), as previously described (Benard et al. ...
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bioRxiv - Genetics 2020Quote: ... We generate all causal variants among common observed variants with MAF > 5% (∼ 40, 000 SNPs in simulated genotypes and 943, 244 SNPs in the SIGMA cohort). To represent environmental stratification ...
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bioRxiv - Microbiology 2020Quote: ... The resin was washed 5 times and transferred to a spin column with 40 μl of 3 X Flag peptide (Sigma, #F4799) for 1 h at 4°C on a rotator ...
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bioRxiv - Immunology 2021Quote: ... and rotated at 37 C for 40 minutes in Collagenase IV Buffer + 2 mg/mL Dispase II (Sigma Aldrich cat. 04942078001). Homogenate was filtered through a cell strainer (Corning cat ...
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bioRxiv - Molecular Biology 2020Quote: ... 10 μg of protein was reduced with 10 mM dithiothreitol (DTT; FUJIFILM Wako) and alkylated with 40 mM 2-chloroacetamide (CAA; Sigma-Aldrich). Digestion was performed according to the PTS protocol (Chang et al. ...
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bioRxiv - Microbiology 2021Quote: ... Protein complexes were isolated from the post nuclear fraction of the NP-40-lysed cells using magnetic beads loaded with Flag-specific monoclonal antibody (MAb) (Sigma, Mo) as described elsewhere (17) ...
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bioRxiv - Neuroscience 2022Quote: ... 300 mM NaCl, 0.5% Nonidet P-40, 2 mM EDTA, 2 mM MgCl2, 0.5 M urea and protease inhibitors; Sigma; cat# P-8340) followed by centrifugation at 4°C for 20 min at 16,100 × g ...
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bioRxiv - Cell Biology 2022Quote: ... medium was exchanged with 900 μl of fresh growth medium before cells were transfected with 40 nM of esiRNA against human FXR (MISSION esiRNA, Sigma, EHU133161). EsiRNA was diluted in Opti-MEM I Reduced Serum Medium (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2022Quote: ... The resulting material was filtered twice on 40-μm cell strainers and depleted of erythrocytes using a red blood cell lysis buffer (Sigma, R7757).
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were then stimulated during 22h with latency-reversing agents (LRAs; 40 nM Romidepsin (Selleckchem) plus 100 nM Ingenol-3-angelate (Sigma-Aldrich)) ...
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bioRxiv - Immunology 2022Quote: ... The supernatants were collected and incubated for 4 h at 4 °C on a rotating wheel with 40 μl of packed anti-FLAG M2 affinity gel (Sigma, #A2220), which had been washed three times with lysis buffer prior to use ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells were washed twice with PBS and lysed in 200µl RIPA buffer (150 mM sodium chloride, 1.0% NP-40 (Sigma-Aldrich, I8896-50ML), 0.5% sodium deoxycholate ...
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bioRxiv - Biochemistry 2022Quote: ... 100% ethanol was added to the proteome digest to a final concentration of 40% v/v before addition of undecanal (EMD Millipore) at an undecanal to peptide ratio of 20:1 w/w and addition of 1 M sodium cyanoborohydride to a final concentration of 30 mM ...
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bioRxiv - Molecular Biology 2022Quote: ... cut into small pieces (< 3mm3) and single cell suspensions were generated by mechanical separation (40 μm Corning cell strainer, Sigma-Aldrich). Pellets were washed once on ice-cold PBS/5 mM EDTA/1x GlutaMax/10 μM Y27632 ...
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bioRxiv - Molecular Biology 2022Quote: ... frozen at -20°C and then individually mixed with a NutriBullet 600 Series after having added 40 mL of distilled water (Sigma-Aldrich) to the 4 weeks old C57BL/6 mice ...
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bioRxiv - Cancer Biology 2022Quote: ... PCD and Trolox were prepared as follows: 40× PCA (protocatechuic acid) stock was made from 154 mg of PCA (Sigma-Aldrich) in 10 mL of Ultrapure Distilled water (Invitrogen ...
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bioRxiv - Pathology 2024Quote: ... Normalised amounts of protein were reduced and alkylated with 10 mM TCEP (Thermo, #77720) and 40 mM chloroacetamide (Sigma, C0267-100G) with incubation at 55 °C for 15 min ...
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bioRxiv - Neuroscience 2024Quote: ... The homogenized tissue was filtered through a 40 µm cell strainer and 5 mL of solution containing 50% iodixanol (OptiPrep Density Medium, Sigma D1556) with 7.5 mM KCl ...
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bioRxiv - Neuroscience 2024Quote: ... Slides containing the sections were immersed for 40 min in 10 mM citrate buffer pH 6 (sodium citrate, Sigma Aldrich, Germany) at 96°C in a water bath ...
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bioRxiv - Neuroscience 2024Quote: ... slices were washed in TB and exposed to a series of glycerol dilutions (25, 40, 55, 70, 85 and 90%; Sigma-Aldrich), immediately mounted on a 0.1% gelatin-coated slide surrounded by agar (4% ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed with PBS containing CaCl2 and MgCl2 followed by the addition of Neutral red solution (40 ug/ml in PBS, Sigma-Aldrich) and incubation at 37°C for 3 hours ...
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bioRxiv - Cell Biology 2024Quote: ... mESCs were split and approximately 6 × 104 cells were plated onto a 12-well plate coated with 1% matrigel in ESCs medium without LIF and the next day medium was replenished with differentiation medium containing 40% MCDB (Sigma-#M6770), 100µM β-mercaptoethanol ...
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bioRxiv - Microbiology 2023Quote: ... Germany) in 500 µL PBS and 40 µL of supernatant was plated on either R2A agar (Sigma-Aldrich, St. Louis, MO) or sheep blood agar plates (Biological Media Services ...
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bioRxiv - Immunology 2024Quote: ... Thymi from 4-month-old to 12-year-old individuals were cryopreserved in liquid nitrogen in the following solution: 95% (PBS-5% Dextran 40 (Sigma-Aldrich)) – 5% DMSO (Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... biotinylation of proteins proximal to V5-APEX2-SENP2 was performed according to previously published protocol [40] with a minor modification where Biotin-Phenol (SML2135, Sigma-Aldrich) was added to the media at a final concentration of 1 mM and the incubation with Biotin-Phenol was extended to 40 min.
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bioRxiv - Immunology 2024Quote: For allergen sensitization 6-10-week-old mice were immunized intranasally with 40 μL of allergen suspension containing 300 μg ragweed pollen (RWP, Greer) and 20 μg ovalbumin (OVA, Sigma Aldrich) in phosphate buffered saline (PBS ...
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T-cell development and activation in humanized mice lacking mouse major histocompatibility complexesbioRxiv - Immunology 2024Quote: ... The tissues were cryopreserved in cryo-medium (40% PBS; 50% Human Serum, Sigma-Aldrich, St. Louis, MO, USA; and 10% DMSO) and stored at -150 °C for further analysis.
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bioRxiv - Immunology 2023Quote: ... guts were transferred to a new 50 mL tubes containing 1x digestion mix (RPMI GlutaMAX™ containing 2% FCS, 1mg/mL of Colagenase VIII [Sigma] and 40 u/mL of DNAse I [Sigma]) and incubated for 20 min at 37 °C sin an orbital shaker at 200 rpm ...
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bioRxiv - Neuroscience 2023Quote: ... Either TA or soleus muscles were surgically exposed and injected with 30-40 μl of 2% (w/v) Evans blue dye solution (Sigma Aldrich) in phosphate buffered saline (PBS ...
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bioRxiv - Plant Biology 2023Quote: ... The samples were resuspended and a homogeneous aliquot of 40 uL was hydrolyzed at 37 °C overnight using a mix 110 uL of µL amyloglucosidase (from Aspergillus niger, Sigma, 10102857001) + α-amylase (from pig pancreas ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... sorted using a BD FACSAriaTM and lysed in 6 M urea/ 2 M thiourea supplemented with 10 mM tris(2-carboxyethyl)phosphine (TCEP) and 40 mM chloroacetamide (CAA) in 75 mM NaCl and 50 mM Tris-HCl (Sigma-Aldrich) and sonicated using a metal tip ...
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bioRxiv - Microbiology 2023Quote: ... in PBS 1X at RT for 40 min and incubated with a blocking buffer containing 3% bovine serum albumin (BSA, Sigma Aldrich) in PBS 1X RT for 40 min ...
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bioRxiv - Molecular Biology 2023Quote: ... the remainder was pre-cleared by co-incubation with 40 µL of PureProteome Protein A/G magnetic beads (Merck Millipore, LSKMAGAG10) per sample and rotation for 1 h at 4°C ...
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bioRxiv - Immunology 2023Quote: ... The cell suspension was passed through 40 µm nylon mesh and subjected to erythrocytes lysis by using HybriMax red blood cell lysis buffer (Sigma Aldrich) at room temperature for 3 minutes ...
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bioRxiv - Evolutionary Biology 2023Quote: ... fixed in 40% isopropanol by three-minute incubation at room temperature and incubated with 3 mg/ml Oil Red O (Sigma, 09755) for 2 hours at room temperature ...
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bioRxiv - Genetics 2023Quote: ... Samples were centrifuged at 500 g for 10 minutes at 4°C using a swinging bucket rotor and the pellets were resuspended in 500 µL of NWB buffer and filtered through a 40 µm cell strainer (Flowmi, Millipore Sigma). 900 µL of sucrose cushion buffer (SCB ...
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bioRxiv - Microbiology 2023Quote: ... at 107 CFUs/mL were incubated in LB buffered medium supplemented or not (control) with 40-80 μg/mL (10-20x MIC) ciprofloxacin (Sigma Aldrich) or 320 μg/mL (10x MIC ...
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bioRxiv - Cell Biology 2023Quote: ... in combination with 150 μg/ml heparin (Gilvasan, 1000 IE/ml) or 40-μM 4-aminobenzoic acid hydrazide (4-ABAH) (Sigma-Aldrich) where applicable.
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bioRxiv - Biochemistry 2023Quote: ... the microtubules were gently resuspended in 300 µL pre-warmed (37 °C) BRB80 containing 20-40 µM paclitaxel (taxol; Sigma-Aldrich).