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Citations for Millipore Sigma :
2551 - 2589 of 2589 citations for Rat FAM24A shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... One to two microliters of plasmid DNA (final concentration of 1 μg/μL) mixed with 0.25% fast green dye (Sigma Aldrich Cat#F7252) was injected into the lateral ventricles using a pulled glass micropipette ...
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bioRxiv - Cell Biology 2024Quote: ... hiPSCs were nucleofected as described above with 2 µg of donor plasmid and 1.5 µl each of 10 µM sgRNA and 10 µM Cas9 (Sigma Aldrich, CAS9PROT-50UG). Once cells reached confluency ...
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bioRxiv - Cancer Biology 2023Quote: ... 250 ng of pTOPflash (wt-TCF) or pFOPflash (mutated-TCF binding site, negative control) reporter plasmids (TOPflash luciferase reporter assay, Merck Millipore, Darmstadt, Germany) were transfected with Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... cells were transfected with either non-targeting siRNA or si-Adipor1 or si-Adipor2 plasmid using lipofectamine 3000 reagents (Sigma-Aldrich, USA) as per manufacturer protocol ...
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bioRxiv - Microbiology 2023Quote: ... active and inactive NS2B(H)-NS3 pro proteases were generated as described previously and cloned into the MCS1 (multiple cloning site 1) of dual protein expression plasmid pETDuet-1 (Novagen, Beijing, China) through restriction sites EcoRI and NotI ...
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bioRxiv - Microbiology 2023Quote: ... were transformed with the lithium acetate method 45 and strains containing the recombinant plasmids were selected on YPD agar with 200 μg/mL geneticin (G418 – Sigma A1720-5G).
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bioRxiv - Cell Biology 2023Quote: ... lentiviral expression plasmids were co-transfected with ΔNRF and VSVG (gifts from J. bear) virus packaging plasmids into HEK293T cells using 50 µg/mL polyethylenimine (PEI)-Max (Sigma-Aldrich Chemistry). Two days later ...
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bioRxiv - Cancer Biology 2024Quote: ... Lentiviral particles were prepared by transfecting 293T cells with expression clones along with the lentiviral envelope and packaging plasmids using X-tremeGENE HP DNA transfection reagent (Sigma-Aldrich 6366546001). The media was refreshed after 24 h ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected with a mixture of 1 μg of plasmid DNA and 1 μl X-tremeGENE™ HP DNA Transfection Reagent (Sigma-Aldrich) per well ...
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bioRxiv - Biochemistry 2021Quote: ... These Wild Type or Mutant Type let-7a-5p plasmids were co□transfected into treated human chondrocytes cells along with NC mimics or KCNQ1OT1 mimic (Sigma□Aldrich; Merck KGaA) using Lipofectamine 6000 following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Cells transfected with the pSpCas9(BB)-2A-Puro plasmid (sgRNAs 1) were treated with 1 μg/mL puromycin (Sigma-Aldrich, 58-58-2) 24 hours after transfection and were selected for 96 hours ...
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bioRxiv - Immunology 2022Quote: ... coli transformed with plasmid pET42 expressing N-terminally GST- and HIS-tagged human IL-18 were grown at 37°C in Lysogeny broth (LB) medium containing kanamycin (25 μg/ml) as selection marker for the pET42a plasmid (Cat No 70561, Novagen, Merck, Overijse, Belgium). When the optical density at 600 nm (OD600 ...
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bioRxiv - Microbiology 2022Quote: ... Sub-confluent T175 flasks of 293T cells (human embryonic kidney cell line) were transfected with the RBD-His tagged plasmid using X-tremeGENE 9 reagent (Millipore Sigma, Cat# 6365809001). The supernatant was collected six days post-transfection and filtered through 0.22μm PES membrane filters (Nalgene) ...
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HIV-1 Nef interacts with LMP7 to attenuate immunoproteasome formation and MHC-I antigen presentationbioRxiv - Microbiology 2019Quote: Nef and LMP7 genes were cloned into pGEX-6P-1 (GE, Boston, MA, USA) and pET-28a plasmids respectively (Novagen, Madison, WI, USA). pGEX-6P-1 empty plasmid and pEGX-6P-1-Nef plasmid were respectively transformed into BL21 (DE3 ...
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bioRxiv - Immunology 2020Quote: ... monoclonal antibodies were produced by transient co-transfection of 293-F cells with human heavy chain and light chain antibody expression plasmids using polyethylenimine (PEI) (Sigma-Aldrich, catalog #408727). Seven days after transfection ...
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bioRxiv - Microbiology 2021Quote: ... Sub-confluent T175 flasks of 293T cells (human embryonic kidney cell line) were transfected with the RBD-His tagged plasmid using X-tremeGENE 9 reagent (Millipore Sigma, Cat# 6365809001). Supernatant was collected 6 days post-transfection and filtered through 0.22 μm PES membrane filters (Nalgene) ...
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bioRxiv - Biophysics 2022Quote: ... were cultivated to 85% confluency in 35 mm culture dishes and transfected with 1.0 μg plasmid DNA using polyethyleneimine (Sigma, St. Louis, MO, USA). After 12 h at 37 °C in 5% CO2 ...
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bioRxiv - Cell Biology 2022Quote: ... from NIH3T3 cells co-expressing Flag-NDRG1 WT or S336A plasmids with GFP-Cdc42 were incubated with 30 μl of ANTI-FLAG® M2 Affinity Gel (Sigma-Aldrich, A2220) and eluted with 3X FLAG® Peptide (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... coli DH10B (remaining plasmids) were used as cloning strains and electroporated with desalted ligation/Hot Fusion reaction (MF-Millipore membrane, VSWP, 0.025 μm).
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bioRxiv - Cell Biology 2024Quote: ... cells were transfected with 5 µg of EPS15 or DECR1 expression plasmids using X-tremeGENE™ 9 DNA Transfection Reagent (Sigma-Aldrich, 6365787001) using 1:3 DNA:reagent ratio ...
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bioRxiv - Immunology 2023Quote: Chitinase activity in the culture supernatant of CHIT1 plasmid-transfected HEK293T cells was measured by using a fluorescence assay kit (Sigma-Aldrich Cat. # CS1030). A 10 μl supernatant sample was mixed with 90 μl substrate solution containing 0.5 mg/ml of 4-methylumbelliferyl N,N’-diacetyl-β-D -chitobioside in an assay buffer provided as part of the kit and in a 96-well plate (black/clear bottom plate ...
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bioRxiv - Cell Biology 2023Quote: ... were generated by PCR and cloned into the pFlag-CMV4 plasmid (for N-terminal Flag fusion in mammalian cells, cat#E7158, Merck Millipore, Burlington, MA, USA). CDS of ARVA ...
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bioRxiv - Molecular Biology 2023Quote: Two-fold dilution series from 40 to 1.25 plasmids per 5µl were prepared using sterile filtered water for molecular biology (Sigma-Aldrich, Saint-Quentin Fallavier, France). Eight repeats of the six dilutions were analyzed per plate ...
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bioRxiv - Genetics 2023Quote: ... WT WTC11 hiPSCs were co-transfected with the pC13N-iCAG-dCas9-VPR-P2A-mCherry repair template and pZT-C13-L1 and pZT-C13-R1 plasmids using GeneJuice transfection reagent (Sigma; cat. no. 70967). Edited hiPSCs were selected using G418 (InvivoGen ...
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bioRxiv - Plant Biology 2023Quote: ... Expression of the plasmid in floral tissue of T2 plants was confirmed by GUS staining assay following the manufacturer’s protocol (Millipore Sigma, Burlington, MA, USA) (Figure S6) ...
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bioRxiv - Molecular Biology 2023Quote: ... Individual cell clones were picked and transfected with pKH011-puro guide plasmids together with piggyBAC transposase and selected with 2 μg/mL Puromycin (Millipore Sigma, Cat# 540411). Cells were subsequently maintained with 6 μg/mL Blasticidin and 2 μg/mL Puromycin ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with siRNAs/plasmid as described earlier and RNA was extracted using GenElute™ Mammalian Total RNA Miniprep Kit (Sigma Aldrich, # RTN70). RNA was reverse transcribe using High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher #4368814) ...
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bioRxiv - Molecular Biology 2020Quote: ChIPs were performed on plasmid shuffled derivatives of KY3232 as described above with an antibody against the FLAG epitope (Sigma-Aldrich, 30 µL A2220) to immunoprecipitate FLAG-Rpb3 ...
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bioRxiv - Cancer Biology 2022Quote: ... MCF7-NLS-eGFP-dCAS9KRAB were then transduced with lentiviral particles containing plasmids expressing individual sgRNAs and selected with Puromycin (Sigma-Aldrich cat no. P8833). For each gene of interest ...
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bioRxiv - Developmental Biology 2021Quote: ... where the Cre enzyme was inserted after excision from pSK-Cre1 plasmid (provided by Clifford Tabbin, Harvard Medical school, diluted in PBS containing 0.05% of Fast Green (Sigma-Aldrich, Saint-Quantin Fallavier, France) was injected into the lateral ventricle of embryos using a Hamilton needle (Dutsher ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The plasmid that contains the kinase domain of human HASPIN was transformed into Escherichia coli strain Rosetta™ 2(DE3) pLysS (Novagen, Madison, WI, USA). The transformed cells were grown at 37□ until OD600 reached 0.8 ...
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bioRxiv - Cell Biology 2023Quote: ... to validate the gene expression the plasmid (vide-supra) was transfected into HEK293 cells using PEI (Sigma-Aldrich/Merck Cat. No. 49553-93-7) following an optimized version of the standard protocol(Rajendra ...
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bioRxiv - Bioengineering 2020Quote: Plasmid DNA was purified after overnight culture in standard lysogeny broth containing 100 µg/mL ampicillin using a miniprep plasmid DNA isolation kit (Sigma Aldrich, St Louis, MO; PLN350) and quantified using spectrophotometry (NanoDrop One ...
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bioRxiv - Molecular Biology 2022Quote: For Generation of stable AKT1 and KLF4 overexpressing cell lines the AKT1 (TRCN0000473539) and KLF4 (TRCN0000492053) overexpression plasmids were obtained from Sigma (Mission TRC3.0, Sigma Aldrich, USA). For lentivirus packing ...
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bioRxiv - Bioengineering 2024Quote: Bacterial expression plasmids carrying CODEs were transformed into homemade competent cells propagated from Rosetta™ 2(DE3)pLysS Singles™ Competent Cells (Millipore Sigma, Cat# 71401). Individual colonies were picked and inoculated in 50 mL Luria Broth (Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... the IA2 geneblock within the Pet53dest plasmid was isolated and mixed with BL21DE3 competent cells and grown prior to IPTG (Millipore Sigma, Cat#367-93-1) and EtOH induction of gene expression ...
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bioRxiv - Molecular Biology 2023Quote: HeLa Flp-In T-REx cells were transfected with pcDNA5-2xFlag-His6-siinsEXOSC10 (WT or K583R) or pcDNA5-2xFlag-His6 (empty vector) plasmids using XtremeGene9® transfection reagent (Sigma Aldrich, St Louis, MO, USA) according to the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2019Quote: ... HEK-293T cells were cultured in suitable medium and transfected by calcium phosphate method with the following plasmids: 10 μg of pLKO.1 (Sigma-Aldrich, St Louis, MO, USA – SHCLNG-NM_000259), containing the shRNA cassette ...
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bioRxiv - Plant Biology 2022Quote: ... were transfected with the purified plasmids (20–30 μg) by adding an equal volume of PEG-Ca solution (40% PEG4000 [Sigma-Aldrich], 0.2 M mannitol, 100 mM CaCl2) for 5–6 min ...