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Citations for Millipore Sigma :
2451 - 2500 of 10000+ citations for AS 1517499 CAS 919486 40 1 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... RBCs were incubated on ice in the presence of 40 μg/ml of the ionophore nystatin (Sigma-Aldrich) in a saline solution of either 75 mM NaCl / 75 mM KCl or 110 mM NaCl / 40 mM KCl in 5 mM Hepes pH 7.4 and 55 mM sucrose ...
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bioRxiv - Genetics 2020Quote: ... and suspended in 1 mL of ice-cold protein extraction buffer (50mM HEPES pH 7.5, 150mM NaCl, 0.02% NP-40, 1mM EDTA, 1mM phenylmethylsulfonyl fluoride [PMSF; Sigma, P7626] ...
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bioRxiv - Biochemistry 2021Quote: ... cells were harvested and lysed in 0.1% NP-40 with phosphatase inhibitor cocktails (Sigma-Aldrich, St. Louis, MO). After brief sonication ...
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bioRxiv - Immunology 2020Quote: ... cells were split into three different conditions light SILAC media (40 mg/mL L-Lysine K0 (Sigma, #L8662) and 84mg/mL L-Arginine R0 (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates (20-40 μg) were subjected to 8-15% SDS-PAGE and transferred to nitrocellulose membranes (Millipore). The membrane was blocked using 5% milk in TBST buffer at room temperature for 1 h ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... compound 21 was administrated at a concentration of 40 mg/kg diluted in sterile corn oil (Sigma-Aldrich) intraperitoneally five times per week starting at the age of 6 weeks ...
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bioRxiv - Physiology 2020Quote: Cells in suspension were washed with PBS and lysed using NP-40 lysis buffer containing proteinase inhibitors (Sigma) and phosphatase inhibitors (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were immersed for 40 min in 10 mM citrate buffer pH 6 (sodium citrate, Sigma Aldrich, Germany) previously heated at 96 °C in a water bath ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... A saturated 40 mM 5-HT stock solution was prepared by dissolving serotonin creatinine sulfate monohydrate (Sigma-Aldrich) in S-medium (67 ...
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bioRxiv - Biochemistry 2021Quote: Cells expressing GRAMD1C-EGFP or GRAMD1C(ΔGRAM)-EGFP were lysed with NP-40 lysis buffer (150 mM, 1.0% NP-40, 50 mM Tris-HCl pH 8.0) supplemented with PhosStop phosphatase inhibitor (Sigma) and complete protease inhibitor cocktail (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... and SARS2-71 [40] anti-S protein antibodies and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Genetics 2021Quote: ... Lysed worm powder was thawed on ice in a final volume of 3.75 ml lysis buffer including 0.5 % v/v NP-40 (Sigma), 0.4 U/μL RNasin (Promega) ...
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bioRxiv - Developmental Biology 2020Quote: ... Westerns were probed with affinity purified rabbit anti-Dsh [24] and Actin AC-40 mouse monoclonal (A4700, Sigma). Detection was using SuperSignal West Dura Extended Duration Substrate (Thermo Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... albicans cells were washed 1x in Sorbitol buffer (1.4 M Sorbitol, 40 mM HEPES-KOH pH 7.5, 0.5 mM MgCl2 10 mM DTT; all Sigma-Aldrich). The pellet was then resuspended in 330 μL Sorbitol buffer and 192 μL of this suspension were mixed with 8 μL of 50 mg/ml 100T Zymolase (2 mg/ml final concentration ...
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bioRxiv - Genomics 2021Quote: ... Mineralized nodule formation was measured by staining cultures with Alizarin Red (40 mM, pH 5.6; Sigma A5533-25G). Reported results were obtained from three biological replicate experiments.
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bioRxiv - Cell Biology 2022Quote: ... Streptozotocin (STZ) to deplete insulin required administration of low doses of STZ (40 mg/kg; Sigma-Aldrich, S0130) injected intraperitoneally once-a-day for 5 consecutive days ...
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bioRxiv - Genomics 2022Quote: ... and 100μL of pre-warmed (65 °C) solution of CTAB/NaCl (40 mM/0.1 M) (Millipore-Sigma, USA) were added ...
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bioRxiv - Genomics 2022Quote: ... and 100μL of pre-warmed (65 °C) solution of CTAB/NaCl (40 mM/0.1 M) (Millipore-Sigma, USA) were added ...
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bioRxiv - Microbiology 2022Quote: ... the mice were orally gavaged with 200 μL of 40 mg/ml FITC-Dextran (4 kDa; Sigma Aldrich) in sterile PBS ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Then 40 ul of each sample was added to 96-well plates and catalase solutions (Sigma, cat#:C1345) in 50 mM potassium phosphate buffer were added to the wells for both standards and samples to lower the background ...
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bioRxiv - Neuroscience 2023Quote: ... Conditioning was performed on groups of around 40-50 flies with 3-octanol (around 95 % purity; Sigma-Aldrich) and 4-methylcyclohexanol (99 % purity ...
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bioRxiv - Physiology 2022Quote: ... females: 9-10 weeks) were treated with a single dose of tamoxifen (40 mg/kg i.p.; Sigma-Aldrich) or vehicle alone ...
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bioRxiv - Bioengineering 2022Quote: ... followed by 40-fold dilution using PBS and ultrafiltration using Amicon Ultra-15-30K centrifugal units (Merck Millipore) for buffer replacement.
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bioRxiv - Cell Biology 2022Quote: ... An equal 20-40 ug of proteins were separated by SDS PAGE and transferred to PVDF membranes (Millipore). Western blots were performed as standard procedures ...
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bioRxiv - Plant Biology 2024Quote: ... The resulting fungal cultures were filtered through a 40-µm strainer (Corning, Cell Strainer; Sigma-Aldrich, Rehovot, Israel) to remove the mycelia and then centrifuged at 3220 g for 10 min to pellet the conidia ...
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bioRxiv - Physiology 2024Quote: ... Culture medium was changed biweekly using 40-µm Corning™ Sterile Cell Strainers (Sigma-Aldrich, St. Louis, MO), which retains the tardigrades while allowing algae and waste to flow through ...
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bioRxiv - Neuroscience 2024Quote: ... Oleic acid was complexed with fresh 40 % HPB (2-Hydroxypropyl)-β-cyclodextrin (H107 Sigma-Aldrich, St Louis, USA). 9 nmols of oleic acid/dose and 2 µl/dose were injected ...
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bioRxiv - Microbiology 2024Quote: ... The cells were washed and the nuclei counter stained with 40-6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich) for 20 min ...
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bioRxiv - Bioengineering 2024Quote: ... 67.3 mM HEPES [pH 8.0], 560 µg/ml glucose oxidase [G2133; Sigma], 40 µg/ml catalase [C1345; Sigma]), and cells were imaged immediately after adding the imaging buffer for no longer than 30 min on a HILO microscope.
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bioRxiv - Neuroscience 2022Quote: ... dissociation and lysed with elution buffer (150 mM NaCl - Sigma, 0.1% NP-40 - Sigma, 5 mM EDTA-Sigma, 50 mM HEPES - Sigma pH7.5 and protease inhibitor cocktail-Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... 0.1% NP-40 - Sigma, 5 mM EDTA-Sigma, 50 mM HEPES - Sigma pH7.5 and protease inhibitor cocktail-Sigma) for 15 minutes at 4°C and centrifuged for 10 minutes at 300xg at 4°C to obtain cell lysates ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μl of 140 nM 48S complex (based on the concentration of the 40S) was applied onto UltrAuFoil R1.2/1.3 300 mesh gold grids pre-covered with graphene oxide (Sigma) suspension made in-house ...
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bioRxiv - Cell Biology 2023Quote: ... 15 U/mL of glutathione reductase and 40 μg/mL of 5,5’-dithiobis(2-nitrobenzoic acid) (Sigma-Aldrich) were dissolved in assay buffer (100 mM K2HPO4/KH2PO4 ...
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bioRxiv - Microbiology 2022Quote: ... and grown in LB broth or on LB agar supplemented with selective antibiotic (40 µg/ml kanamycin; Sigma) as indicated ...
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bioRxiv - Immunology 2023Quote: ... Affinity columns were prepared as follows: 40 mg of Cyanogen bromide-activated-Sepharose 4B (Sigma-Aldrich, Cat# C9142) were activated with 1mM hydrochloric acid (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... The mixed cultures (5 μL) were spotted on 1.5% agarose supplemented with 40 µg/mL CPRG (Sigma-Aldrich) in 96 wells plates ...
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bioRxiv - Cell Biology 2022Quote: ... Tissues were cultured in DMEM supplemented with L-Proline (40 μg/mL, Sigma-Aldrich, St. Louis, MO, USA), Insulin-Transferrin-Selenium (1% ...
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bioRxiv - Immunology 2023Quote: ... The cell suspension was then layered on discontinuous 60% and 40% percoll gradients (Sigma-Aldrich, Saint Louis, Missouri) and centrifuged for 30 minutes at 930xg with the brakes off ...
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bioRxiv - Immunology 2023Quote: ... coverslips were washed with PBS for 2 minutes at RT and mounted with Mowiol 40-88 (Sigma-Aldrich). Confocal microscopy images were acquired using a Zeiss LSM 880 instrument ...
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bioRxiv - Immunology 2023Quote: ... coverslips were washed with PBS for 2 minutes at RT and mounted with Mowiol 40-88 (Sigma-Aldrich). Confocal microscopy images were acquired using a Zeiss LSM 880 instrument ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were incubated with 40 μL Anti-FLAG M2 affinity gel (cat A2220, Sigma-Aldrich, St. Louis, US) and incubated at 4°C overnight ...
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A synthetic elastic protein as molecular prosthetic candidate to strengthen vascular wall elasticitybioRxiv - Cell Biology 2023Quote: ... Samples were coacervated at 40°C in silicone molds in presence of 50 mM aqueous genipin (Sigma-Aldrich) and cross-linked overnight at 40°C ...
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bioRxiv - Molecular Biology 2024Quote: ... The cell pellet was resuspended in 500 µl of ice-cold PBS containing 0.1% NP-40 (Millipore Sigma), and 100 µl of the cell suspension were collected as the whole cell fraction ...
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bioRxiv - Cancer Biology 2024Quote: ... nuclei were incubated in mild lysis buffer (10 mM Tris-HCl pH8.5, 10 mM NaCl, 0.5% NP-40) containing protease inhibitor (PI, Sigma), followed by wash buffer (10 mmol/L Tris-HCl pH8.5 ...
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bioRxiv - Biochemistry 2024Quote: ... 160 µL per condition were mixed with 40 µL of 5x Laemmli buffer [10% glycerol (Sigma-Aldrich, 15523), 1% beta-mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... We applied 3 μl of 140 nM 40S ribosomal complexes onto UltrAuFoil R1.2/1.3 300 mesh gold grids pre-covered with graphene oxide (Sigma) suspension made in-house as described before 16 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 2013) by dissolving the metabolite pellet carefully in 20 µL of 40 mg of methoxyamine hydrochloride (Sigma Aldrich) in 1 mL pyridine (anhydrous >99,8 % ...
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bioRxiv - Immunology 2024Quote: Total protein (40 µg/lane) was separated by SDS-PAGE and transferred onto the PVDF membrane (Millipore, USA). After the membranes were blocked with 3% BSA in PBST containing 0.05% Tween-20 ...
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bioRxiv - Molecular Biology 2024Quote: ... Gels ran for 35 to 40 minutes in MES-buffer (50 mM MES (SIGMA, Cat. No. M3671-250G), 50 mM Tris base (Fisher Bioreagents ...
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bioRxiv - Cancer Biology 2024Quote: ... were lysed in ice-cold lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% NP-40: Sigma). Transposition was performed directly on nuclei using 25 μl tagmentation reaction mix (Tagment DNA Buffer #15027866 ...