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Citations for Millipore Sigma :
201 - 250 of 10000+ citations for Mumps virus lysate Isolate 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... An empty TRC1 lentiviral construct was used as the Control virus (Sigma).
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bioRxiv - Cell Biology 2024Quote: ... Cells were transduced with virus along with 10 μg/mL polybrene (Sigma). Following transduction ...
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bioRxiv - Genomics 2024Quote: ... virus-containing supernatant was filtered through a 0.45 μm filter (Merck Millipore), aliquoted ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein lysates were prepared with HENS buffer (250 mM HEPES, pH 7.7, 1 mM EDTA, 0.1 mM neocuproine [Sigma, P9599], 1% SDS) and protease inhibitor cocktail ...
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bioRxiv - Genetics 2020Quote: ... 450 µL of the cleared lysates were incubated with 1 µL of primary antibody (α-Flag M2 (Sigma-Aldrich; 1:400)) at 4 °C for 3 hours ...
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bioRxiv - Neuroscience 2022Quote: ... The standard settings for the 66-440 kDa separation modules were used with 0.2 mg/ml of each lysate and anti-HTT (Ab1, 1:50) plus anti-Vinculin (Sigma, 1:2000) antibodies ...
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bioRxiv - Immunology 2019Quote: ... duplicate wells of a 12-well plate were seeded with 1 ml/well of the cell suspension (1 × 106 cells/ml) and virus was added in the presence of 6 μg/ml polybrene (Sigma-Aldrich, #107689) (multiplicity of infection of 25) ...
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bioRxiv - Cell Biology 2022Quote: ... the virus-containing supernatant was filtered and used to infect WM115 cells in the presence of 1 μg/mL polybrene (Sigma-Aldrich, H9268). This was repeated with fresh viral supernatant 48 hours later ...
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bioRxiv - Pathology 2022Quote: ... and a mouse monoclonal antibody raised against Simian Virus-40 (SV-40, Macaca mulatta polyomavirus-1, Betapolyomavirus macacae) LT antigen (CalBiochem®, Clone PAb416, Sigma-Aldrich) was used as primary antibody at a 1:200 dilution ...
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bioRxiv - Microbiology 2022Quote: ... beginning with a virus stock dilution of 1:5 in complete DMEM media as mentioned above supplemented with DEAE-Dextran (Sigma Aldrich #30461) at the final concentration of 40 mg /ml ...
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bioRxiv - Systems Biology 2023Quote: ... K562 cells were transduced in 6-well plates by mixing the cells and virus with 1:500 dilution of 4 mg/mL Polybrene (Sigma H9268-5G). For spinfection ...
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bioRxiv - Microbiology 2020Quote: ... Aliquots of the environmental water samples used to isolate bacteria were centrifuged and filtered (0.22 μm Millipore membrane) and then spotted on the lawns in order to identify the bacterial isolates that could be infected by the phages present in the samples ...
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bioRxiv - Biophysics 2020Quote: ... The coupled enzymatic reaction contained ~2 units of pyruvate kinase/lactic dehydrogenase (rabbit muscle isolate mixture, Sigma Aldrich) 50 mM KPi ...
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bioRxiv - Microbiology 2022Quote: ... A PgVV-only lysate was obtained by 0.2 µm filtering of a mixed lysate (Millex, SLGV033RS, Merck Millipore), TFF concentration (Repligen N06-E100-05-N ...
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bioRxiv - Biochemistry 2020Quote: ... Clarified lysate was loaded onto a gravity column containing 1 mL HIS-Select Nickel Affinity Gel (Sigma) pre-equilibrated with Resuspension Buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 mg of lysate was incubated with pre-washed anti-FLAG M2 affinity gel (Sigma-Aldrich, #A2220) for 2 hours at 4°C with gentle nutation ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mg of lysate was incubated overnight with 20µl anti-Flag M2 magnetic Dynabeads (Sigma-Aldrich/Merck) under shaking ...
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bioRxiv - Microbiology 2019Quote: ... Cell lysates were incubated 45 min at 37 °C with 0.6 mg.mL−1 fructose-6-phosphate (Sigma) and 0.6 mg.mL−1 glutamine (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... The lysates were then subjected to Western blot with anti-FLAG M2 mAb (F3165, Sigma, 1:3,000), anti-HA mAb (HA.C5 ...
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bioRxiv - Cell Biology 2022Quote: Flag-tagged proteins were captured from 1 mg total cell lysate using anti-Flag affinity matrix (Sigma) for 1 hour at 4°C ...
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Myosin VI regulates ciliogenesis by promoting the turnover of the centrosomal/satellite protein OFD1bioRxiv - Cell Biology 2021Quote: ... Anti-Flag IP was performed by incubating 1 mg lysate with anti-Flag M2 conjugated beads (Sigma) for 2 hours at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Whole-cell lysates were incubated for 1 h with ANTI-FLAG® M2 Affinity Gel (Sigma; A2220) followed by extensive washing ...
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bioRxiv - Neuroscience 2023Quote: ... and 1% Triton™ X-100) and cleared lysates were added to anti-FLAG magnetic beads (Sigma). Protein bound beads were washed with 3 washes with Tris-buffered saline (TBS ...
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bioRxiv - Biochemistry 2023Quote: ... Cleared RPE and retinal lysates were prepared in RIPA buffer with 1% protease inhibitor mixture (Sigma; P8340) and 2% phosphatase inhibitor mixture 2 (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... Supplemented lysate was then mixed with 1 mL of pre-washed anti-FLAG M2 agarose beads (Sigma) and allowed to bind under nutation at 4℃ for 2 hrs ...
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bioRxiv - Immunology 2023Quote: ... lysates were incubated with anti-FLAG matrix (M8823, Millipore; pre-blocked with 1% BSA-PBS for 1h) for 2h ...
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bioRxiv - Genetics 2020Quote: ... The lysate was treated with Benzonase (Sigma) together with MgCl2 (final concentration ...
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bioRxiv - Cell Biology 2021Quote: ... The lysates were treated with Benzonase (Sigma) together with MgCl2 (final concentration ...
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bioRxiv - Molecular Biology 2022Quote: ... lysates were applied to Glutathione-Agarose (Sigma), washed with 20 CV of resuspension buffer with 1% Tween-20 ...
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bioRxiv - Genomics 2023Quote: The lentiviral titer was determined by transducing 400,000 cells with increasing volumes (0, 1, 2, 5, 10, 20, and 40 μl) of concentrated virus and polybrene (6 μg/ml; Millipore, TR-1003-G). 48 hours after the transduction ...
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bioRxiv - Microbiology 2024Quote: ... ½-log serially diluted influenza viruses in virus growth medium plus trypsin (DMEM, 0.1% BSA, 1 µg/mL TPCK-treated trypsin (Sigma, St. Louis, MO, USA)) were added to confluent monolayers of MDCK-SIAT1 cells (37 ...
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Targeted rescue of synaptic plasticity improves cognitive decline after severe systemic inflammationbioRxiv - Neuroscience 2021Quote: ... Virus was then concentrated using Amicon Ultra-4 centrifugal filter units (Merck-Millipore) and the solution was filtered with a 0.2 µm Acrodisc syringe filter (Sigma-Aldrich).
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bioRxiv - Molecular Biology 2019Quote: ... Virus was thawed at 37°C and 5μl 10mg/ml polybrene (Sigma H9268) was added to the virus ...
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bioRxiv - Biochemistry 2021Quote: ... Cholesteryl hemisuccinate (CHS) and tobacco etch virus (TEV) protease were from Sigma-Aldrich.
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bioRxiv - Neuroscience 2021Quote: ... Virus was then concentrated and purified via Iodixanol gradients (“Optiprep” Sigma D1556-250mL). Centrifuge the gradients for 90 min at 46,500 rpm at 16 °C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... virus containing supernatant was harvested and filtered (0.45 μm, Millex-HV Filter; Millipore). Subsequently ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were infected with virus in the presence of 8μg/ml polybrene (Sigma).
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bioRxiv - Cell Biology 2022Quote: ... Live filtered virus was used to transduce cells with polybrene (10μg/ml, Sigma).
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bioRxiv - Cell Biology 2020Quote: ... the virus was concentrated using Amicon ultra-15 centrifugal filter units (Millipore Sigma) by centrifuging at 3000 rpm and stored at -80°C until use ...
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bioRxiv - Immunology 2019Quote: ... Virus-containing supernatants were filtered through a 0.45-μm-pore-size filter (Millipore) and supplemented with polybrene (8 μg/ ml ...
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bioRxiv - Cell Biology 2019Quote: ... Virus was resuspended by fresh MEFs medium with 8 μg/ml polybrene (Sigma).
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bioRxiv - Microbiology 2020Quote: ... 100 μl of virus preparations were treated with 25 units of Benzonase (Sigma) for 30 min before RNA isolation.
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bioRxiv - Cancer Biology 2020Quote: ... the virus was harvested from the medium filtered by 0.45 mm Steriflipfilter (Millipore). Virus infection was carried out by incubating cells with cell medium containing indicated virus and 5 μg/ml polybrene (Sigma ...
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bioRxiv - Microbiology 2020Quote: Cells or virus were lysed with RIPA buffer and protease inhibitor cocktail (Sigma). Protein loaded from viral lysates were normalized to p24 antigen content ...
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bioRxiv - Immunology 2021Quote: ... Virus and cell pellets were solubilized in lysis buffer [10mM iodoacetamide (Sigma-Aldrich), CompleteTM protease inhibitor tablets (Roche) ...
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bioRxiv - Immunology 2021Quote: ... The virus-plasma-cell mixtures were plated in white polystyrene plates (Millipore-Sigma) and cultured at 37°C 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... The virus-plasma-cell mixtures were plated in white polystyrene plates (Millipore-Sigma) and cultured at 37°C 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... Supernatant was aspirated and cells resuspended in virus medium containing TPCK-trypsin (Sigma), 20 mM HEPES (Corning ...
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bioRxiv - Bioengineering 2023Quote: ... filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1 ml aliquots in cryovials at -80°C.
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bioRxiv - Bioengineering 2023Quote: ... filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1 ml aliquots in cryovials at -80°C.