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Citations for Millipore Sigma :
51 - 100 of 10000+ citations for Mumps virus lysate Isolate 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: Virus was produced by polyethylenimine (PEI; Sigma-Aldrich) transfection of 89-90% confluent HEK293T cells with the viral vector together with the lentiviral packaging plasmids ...
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An endothelial SOX18-mevalonate pathway axis enables repurposing of statins for infantile hemangiomabioRxiv - Molecular Biology 2024Quote: ... or an empty vector control virus (Sigma SHC001V) followed by puromycin (1 μg/mL ...
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bioRxiv - Microbiology 2019Quote: ... Stock solutions of the isolates were kept in 50% glycerol (Sigma, Dorset, UK) and Middlebrook 7H9 Broth and stored at −80 °C ...
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bioRxiv - Microbiology 2020Quote: ... Gram stain of each isolate was performed using a standard protocol (Sigma, UK) and visualised under a Motic Trinocular light microscope mounted with a Moticam X Lite camera (Motic ...
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bioRxiv - Microbiology 2020Quote: Total SpeB activity was measured in individual isolates by hydrolysis of azocasein (Sigma) by methods previously described (21 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The suspension from each isolate was amended with 0.1% Tween 20 (Sigma-Aldrich) prior to inoculation.
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bioRxiv - Cell Biology 2021Quote: ... 1 mg of cell lysates were incubated with Flag-M2 (M8823, Sigma) or GFP (gtma ...
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bioRxiv - Molecular Biology 2019Quote: ... Tissue lysates were mixed with 1-bromo-3-chloropropane (B9673, Sigma-Aldrich) at a ratio of 1:4 and the RNA containing aqueous/organic phase was separated using 5Prime PhaseLock Gel Heavy (#2302830 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Lysate was then acidified to 1 % trifluoroacetic acid (Sigma Cat. No. 302031) for DNA hydrolysis ...
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bioRxiv - Microbiology 2020Quote: ... Airway Chips infected with influenza virus (MOI = 0.1) were treated with 1 µM oseltamivir acid (Sigma-Aldrich) under flow (60 µl/h ...
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bioRxiv - Microbiology 2021Quote: ... and 10% formamide in DEPC-treated H2O) with anti-influenza A virus NP antibody (Millipore, 1:2,000) and FISH probes ...
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bioRxiv - Microbiology 2022Quote: ... virus was preincubated for 1 hr at 4 °C with 100 μg/mL soluble H (Sigma H4784), HS (Sigma H7640) ...
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bioRxiv - Cancer Biology 2023Quote: ... the virus was removed and replaced with normal growth medium containing 1 mg/ml puromycin from Sigma. The puromycin selection was used to kill off any uninfected control cells ...
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bioRxiv - Microbiology 2023Quote: ... the virus suspension was replaced with 1mL/well of overlay medium (OM) containing 1% methylcellulose (Sigma, USA) in MM ...
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bioRxiv - Cancer Biology 2021Quote: ... To transduce cells with virus cells were treated with both virus (MOI 0.5-1.0) and 5 μg / mL Polybrene infection reaction (EMD Millipore, TR-1003). For pLKO.1 vectors ...
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bioRxiv - Neuroscience 2021Quote: ... Lysates were incubated O/N with prewashed 1:1 anti-HA agarose beads (Sigma, Cat# A2095) at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Aggregated virus was removed by a 0.22µm filter (Millipore). Sub-confluent monolayer of MEFs cultured in DMEM ...
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bioRxiv - Physiology 2020Quote: ... Virus was filtered with a 0.45mm OVDF filter (Millipore). Supernatants were collected and purified using PEG-it (System Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... supernatant containing virus was filtered with 0.22μm filters (Millipore) and store at −80◻ in aliquots.
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bioRxiv - Cancer Biology 2023Quote: ... adherent cells were infected with COL7A1 virus (Sigma Aldrich) (1–5 MOI (multiplicity of infection) ...
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bioRxiv - Molecular Biology 2023Quote: ... and the virus was concentrated using polyethylglycol (Sigma, P4338). Cells were infected in medium containing 5 μg ml−1 polybrene (Millipore ...
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bioRxiv - Microbiology 2019Quote: ... coli 10129 and AMC-resistant isolates were grown on LB agar (Lennox) (Sigma, UK) for colony counting during MIC determination.
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bioRxiv - Microbiology 2021Quote: ... aureus isolates were grown overnight in 5 ml of Tryptic-Soy Broth (TSB; Sigma) in a 30 ml glass tube at 37°C with shaking at 180 rpm ...
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bioRxiv - Genomics 2019Quote: ... This isolate was propagated on potato dextrose agar (PDA; Sigma Aldrich, St. Louis, USA) and incubated at 24°C using a 12 h:12 h light:dark photoperiod ...
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bioRxiv - Microbiology 2023Quote: ... blood of healthy volunteers was used to isolate PBMCs by Histopaque-1077 (Sigma-Aldrich) density gradient centrifugation ...
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bioRxiv - Neuroscience 2023Quote: ... GBS clinical isolate COH1 (serotype III) was grown in Todd-Hewitt broth (THB)(Sigma) at 37° C and growth was monitored by measuring optical density at 600 nm (OD600) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Fungal isolates were grown on Sabouraud agar medium (Sigma Aldrich, St. Louis, MO, USA) for 48 h at 30°C and resuspended in distilled water at a concentration of 1-5 × 105 CFU/ml before testing ...
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bioRxiv - Microbiology 2023Quote: ... tuberculostearicum isolates were grown in overnight liquid culture consisting of BHI broth (Sigma-Aldrich), augmented with 1% RPI Tween80 ...
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bioRxiv - Microbiology 2023Quote: The isolates were stored at −80 °C in Luria-Bertani (LB) broth (Sigma Aldrich) (W/V ...
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bioRxiv - Cell Biology 2020Quote: ... Complementary DNA was reverse transcribed from 1 μg total RNA with Moloney murine leukemia virus reverse transcriptase (Sigma) using dNTP (Promega ...
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bioRxiv - Cancer Biology 2022Quote: ... filtered (0.45 µm) and CDX cells were infected with 1 mL virus containing 12 µg/mL polybrene (Sigma), followed by selection with Puromycin (Merck ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were transduced with lenti-shPGC1α RNA virus with 1:2000 dilution polybrene (Millipore, TR-1003-G) for 24 h and then media was changed ...
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bioRxiv - Microbiology 2023Quote: ... Virus-plasma mixture were aspirated and 2ml of 1% methylcellulose (combination of 2% methylcellulose (Sigma, Cat#435244-250G) and equal parts of 2X MEM (Gibco ...
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bioRxiv - Immunology 2023Quote: ... Pseudotyped ebola virus were incubated at 37°C for 1 h with 200 μg/ml thermolysin (Sigma, T7902). The reaction was stopped by addition of 400 μM Phosphoramidon (Sigma ...
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bioRxiv - Immunology 2019Quote: ... The leukocytes-rich pack was used to isolate peripheral blood mononuclear cells (PBMCs) by Ficoll-Histopaque 1077-1 (Sigma-Aldrich, St. Louis, MO) density gradient ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Lysates and concentrated particles were analyzed for FLAG (Sigma–Aldrich; A8592; 1:10,000), GAPDH (Sigma–Aldrich ...
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bioRxiv - Microbiology 2019Quote: ... One tube of lysate was treated with 1 μL of trehalase (Sigma-Aldrich) enzyme or vehicle control ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein lysates were prepared in RIPA buffer (containing protease (1:100; Sigma, P8340) and phosphatase inhibitors (1:500 ...
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bioRxiv - Cancer Biology 2020Quote: ... Lysates were collected RIPA buffer containing 1 × protease inhibitor cocktail (P8340; Sigma-Aldrich). Twenty micrograms of protein per sample was loaded on polyacrylamide–SDS gels ...
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bioRxiv - Immunology 2021Quote: Bacterial cultures or cell lysates were acidified to pH=1 using HCl (Sigma) and extracted twice with ethyl acetate (Sigma) ...
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bioRxiv - Molecular Biology 2022Quote: These His-tagged protein lysates were combined with 1 ml nickel beads (Sigma) and incubated at 4 °C/2 hours/rolling ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were incubated with 1 μg anti-FLAG-BioM2 antibody (#F9291, Sigma-Aldrich) or without antibody for 3 h at 4°C under constant rotation ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were treated with 1 U/mL RNase T1 (Sigma-Aldrich, cat#R1003) for 15 minutes at 22 ℃ ...
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bioRxiv - Microbiology 2020Quote: ... lysates were diluted 1:1 in RIPA buffer and precleared with protein A agarose beads (Millipore-Sigma) for 60 min at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... lysates were diluted 1:1 in RIPA buffer and precleared with protein A agarose beads (Millipore-Sigma) for 60 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... The media was replaced after 24 hours with 1 mL growth media mixed with 1 mL of virus media and 8 μg/mL of Polybrene (Merck Millipore, TR-1003-G). One day later ...
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bioRxiv - Plant Biology 2019Quote: ... and 1 mM DTT) for 2 hours at 30°C with native Tobacco Etch Virus (TEV) protease (Sigma-Aldrich). The purified recombinant proteins were concentrated using Amicon centrifugal filters following the manufacturer recommendations (Millipore) ...
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bioRxiv - Microbiology 2019Quote: ... cDNA was generated from 1 μg of RNA using Moloney murine leukaemia virus (M-MLV) reverse transcriptase (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: RNA was extracted from CsCl purified reovius preparations (~1×1010 virus particles) using TRI Reagent LS (T3934, Millipore Sigma) as per manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2020Quote: ... the virus was removed and the cultures overlaid with a viscous solution of 1% (w/v) carboxymethylcellulose (Sigma #C4888) in DMEM-F12 (8% FBS ...