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Citations for Millipore Sigma :
151 - 200 of 10000+ citations for Mumps virus lysate Isolate 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... the virus was mixed with fully supplemented DMEM (Sigma-Aldrich, D6429) and 8 μg/ml Polybrene (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated for 24-48 h with the virus supernatant supplemented with 8 µg ml-1 polybrene (Sigma-Aldrich #H9268) before being subjected to further experimental procedures.
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bioRxiv - Microbiology 2020Quote: ... Duplicate cDNA preparations from each sample were generated from 1 μg of RNA using Moloney murine leukaemia virus (M-MLV) reverse transcriptase (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... all transductions were performed in multiple plates with a range of virus volumes and 8 μg mL−1 polybrene (EMD Millipore), but only the pool with the most optimal transduction efficiency was expanded and/or screened.
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bioRxiv - Microbiology 2021Quote: 3 μl of A/PR/8/34 (H1N1) influenza virus mixed with 1 μl of 5x BSA-coated 10 nm colloidal gold (Sigma-Aldrich) were applied to freshly washed and glow-discharged R2/2 holey carbon copper grids (Quantifoil) ...
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bioRxiv - Microbiology 2021Quote: A549-ACE2 cells were infected by incubating the virus for 1h with the cells maintained in DMEM supplemented with 1 μg/ml TPCK-trypsin (Sigma #4370285). The viral input was then removed and cells were kept in DMEM supplemented with 2% FBS ...
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bioRxiv - Microbiology 2021Quote: Duplicate cDNA preparations from each sample were generated from 1 μg of RNA using Moloney murine leukaemia virus (M-MLV) reverse transcriptase (Sigma−Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Duplicate cDNA preparations from each sample were generated from 1 μg of RNA using Moloney murine leukaemia virus (M-MLV) reverse transcriptase (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... The swabs were placed into 1 ml of Trizol or 2 ml of virus transport medium comprising tissue culture medium 199 (Sigma-Aldrich) supplemented with 25mM HEPES ...
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bioRxiv - Microbiology 2022Quote: ... A 500 μL aliquot was collected every 10−50 s (depending on the virus) and mixed with 5 μL of 5,000 mg L−1 sodium thiosulfate (Sigma-Aldrich, Germany) to instantly quench the residual free chlorine ...
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bioRxiv - Microbiology 2022Quote: KSHV BAC16 virus stock was prepared by inducing lytic cycle in iSLK-BAC16 cells with Doxycycline (1 µg/ml; Sigma-Aldrich) and sodium butyrate (1 mM ...
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bioRxiv - Neuroscience 2022Quote: ... RabiesΔG-mCherry virus (titer of around 110 TU/ml) was mixed at a 2:1 ratio with silk fibroin (Sigma #5154) to make rabies injection solution (Jackman et al. ...
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bioRxiv - Microbiology 2022Quote: ... the cells were washed three times with PBS and virus media with 1 μg/ml TPCK treated trypsin (Sigma-Aldrich, USA) was added ...
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bioRxiv - Biochemistry 2024Quote: ... The HeLa cells were transduced in 6-well plates with 1 mL of unconcentrated virus + 10 µg/mL polybrene (TR-1003-G, Sigma Millipore) per well for 24 h ...
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bioRxiv - Neuroscience 2019Quote: ... We then removed the supernatant and incubated the lysates on ice for 1 hour with rabbit antibody to D2 receptors (1 μg per 100 μL, Millipore). Anti-rabbit Ig IP beads were added and samples were incubated overnight at 4°C with gentle agitation ...
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bioRxiv - Biochemistry 2023Quote: ... RGS9 was immunoprecipitated from the lysate by adding 1 μg of a HA antibody (Sigma Aldrich 1186723001, 1:400 IP) following the same protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... at 1.5 × 105 cells per well and infected with 100-300μl of low-titer virus in the presence of infection mix (1/10 dilution of polybrene [10 mg ml−1 Sigma; 107689-100MG in PBS] in FBS[-]), by centrifuging plates at 1,100 g for 30 min at 37 °C in a Thermo Heraeus Megafuge 40R centrifuge ...
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bioRxiv - Genomics 2023Quote: ... The lysate was benzonase (Millipore, 70664) treated for 30 min ...
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bioRxiv - Biophysics 2021Quote: ... A single colony isolate was grown overnight and then sub-cultured in tryptone media (0.015g/l; Sigma) till mid-log phase (OD 600 at 200 rpm ...
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bioRxiv - Microbiology 2021Quote: Overnight cultures of all 37 isolates were grown in M9 minimal media (2x M9, Minimal Salts (Sigma), 2 mM MgSO4 and 0.1 mM CaCl2 ...
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bioRxiv - Microbiology 2023Quote: ... DK1042 and soil isolates were routinely cultured in tryptone soy broth (CASO broth; Sigma-Aldrich, Darmstadt, Germany), LB ...
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bioRxiv - Biochemistry 2023Quote: ... was used to isolate peroxisomes from primary hepatocytes by referring to the protocol provided by Sigma-Aldrich. The isolated subcellular fractions were lysed with lysis buffer and analyzed by Western Blot.
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bioRxiv - Microbiology 2023Quote: ... and Enterobacter hormaechei patient isolates was prepared using the GenElute Bacterial Genomic DNA Kit (Sigma-Aldrich, UK), and the Gram-negative DNA extraction protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... DNA and RNA were removed from the cell lysate using benzonase (1 U/ml, Merck Millipore) for 10 min on room temperature (RT) ...
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bioRxiv - Immunology 2019Quote: ... We prepared protein lysates by scraping the cells in cold lysis buffer containing 1% Igepal (Sigma) and CompleteTM protease inhibitor cocktail and preclearing by centrifugation at 14,000 g for 10 min at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Lysates were incubated with Benzonase (1 μl; 250 units/mL stock solution; Sigma, St. Louis, MO) for 10 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mg of cleared lysate was incubated with CaM agarose resin (Sigma Aldrich, San Luis, USA) for 2 hours at 4°C under gentle rotation ...
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Pseudohypoxic HIF pathway activation dysregulates collagen structure-function in human lung fibrosisbioRxiv - Cell Biology 2021Quote: ... Western blotting of cellular lysates was performed for β-actin (1:100.000, Sigma-Aldrich, Poole, UK), LOXL2 (1:1000 ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysate was applied to 1 mL bed volume (BV) Anti-M2 Flag resin (Sigma-Aldrich) in buffer D (50 mM Hepes-KOH pH 7.6 ...
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bioRxiv - Cell Biology 2021Quote: ... 500-μg of pre-cleared lysates were immunoprecipitated with 1-μg anti-FLAG antibody (Sigma-Aldrich) overnight ...
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bioRxiv - Cell Biology 2023Quote: ... the cell lysates were pre-incubated with 1 µL of anti-Flag M2 antibody (Sigma-Aldrich) for 2 hours at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... the clarified lysate was treated with 50U/ml of benzonase (Novagen; 1 h at 37 °C) and centrifuged ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The lysate was sonicated then incubated with 500 units/1 L culture Benzonase Nuclease (Sigma-Aldrich) for 30 minutes then with 5 units/1 L RQ1 RNase-free DNase (Promega ...
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bioRxiv - Cancer Biology 2021Quote: ... Virus was then filtered with a 0.45 µm PVDF filter (SLHV013SL, Millipore) and mixed with polybrene (TR-1003-G ...
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bioRxiv - Genetics 2021Quote: ... Increasing amounts of virus and 8 μg/ml polybrene (hexadimethrine bromide, Sigma) were added to each well ...
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bioRxiv - Microbiology 2020Quote: ... The virus was propagated once in Vero E6 cells in DMEM (Sigma) supplemented with 2% fetal bovine serum (Gibco) ...
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bioRxiv - Systems Biology 2020Quote: ... Virus was concentrated using Amicon Ultra-15 Centfigiual Filter Concentrators (Millipore UFC910008). Virus was titered by plaque assay ...
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bioRxiv - Microbiology 2020Quote: ... virus-containing supernatants were filtered through a 0.45 μm membrane (Merck Millipore). The amount of virus in the supernatants was quantified by RT assay ...
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bioRxiv - Cell Biology 2022Quote: ... Virus amplification was performed in Sf9 TriEX cells (Novagen / Merck #71023-3) grown in TriEx media (Novagen / Merck #71022) ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibody for DENV was MAB8705 Anti-dengue virus complex antibody (Millipore); secondary antibody was the Goat anti-mouse Alexa Fluor 488 ...
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bioRxiv - Immunology 2022Quote: ... virus particles were precipitated by adding 50% (w/v) PEG6000 (Sigma-Aldrich) in NT buffer (150 mM NaCl ...
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bioRxiv - Neuroscience 2021Quote: ... Virus was backfilled into a pipette of mineral oil (M5904, Sigma-Aldrich). We injected 400nl virus into the identified C2 barrel column through a single injection site over 4 min at 300μm below pia ...
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bioRxiv - Immunology 2020Quote: Inactivated virus was concentrated using Amicon Ultra Centrifugal Filter units (Millipore Sigma) at 100k cutoff ...
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bioRxiv - Microbiology 2020Quote: ... Unabsorbed virus were removed and cells were overlaid with MEM (Sigma-Aldrich) containing 0.65% agarose (Nacalai tesque ...
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bioRxiv - Cancer Biology 2023Quote: ... The virus was concentrated using Amicon Ultra filters (Millipore cat. no. UFC910024) until they have about 1.5ml of concentrated virus ...
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bioRxiv - Cell Biology 2023Quote: ... where cells and virus were mixed with polybrene (Millipore, TR-1003-G) in antibiotic-free media at 2 M cells per well of a 12-well plate ...
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bioRxiv - Cell Biology 2023Quote: ... where cells and virus were mixed with polybrene (Millipore TR-1003-G) in antibiotic-free media at 2 M cells per well of a 12-well plate ...
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bioRxiv - Neuroscience 2023Quote: ... The virus-infected GL261 cells were trypsinized with 0.25% trypsin (Sigma-Aldrich) 72 hours after infection ...
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bioRxiv - Cell Biology 2023Quote: ... Virus was then filtered with a 0.45 µm PVDF filter (SLHV013SL, Millipore) and mixed with 4-fold volume of fresh media containing 12.5 µg/ml polybrene (TR-1003-G ...
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bioRxiv - Cell Biology 2023Quote: ... Virus was then filtered with a 0.45 µm PVDF filter (SLHV013SL, Millipore) and mixed with 4-fold volume of fresh media containing 12.5 µg/ml polybrene (TR-1003-G ...