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Citations for Millipore Sigma :
301 - 350 of 10000+ citations for Mumps virus lysate Isolate 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Cleared lysate was incubated with benzonase (Sigma-Aldrich) at 8°C for 20 min and then applied on a 5 ml MBP-trap column (Cytiva) ...
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bioRxiv - Cell Biology 2022Quote: ... EVI1 or MECOM virus in 12 well plates with 8µg/ml of polybrene (Millipore), spun at 2,000 r.p.m ...
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bioRxiv - Microbiology 2021Quote: ... the virus was concentrated using a 30-kDa Amicon Ultra-15 Centrifugal Filter (Millipore). The viral stocks were stored at -80°C ...
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bioRxiv - Microbiology 2020Quote: ... the virus inoculum was removed and phenol-red free DMEM supplemented with3% BSA (Sigma), 100U/ml pen/strep (Life Technologies) ...
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bioRxiv - Biophysics 2021Quote: ... the virus supernatant was concentrated using 8% polyethylene glycol (PEG)-6,000 (Sigma-Aldrich, U.S.A). The pellet was resuspended in TNE buffer (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... Virus stocks were filtered via centrifugation with Amicon Ultra-15 Centrifugal filter unit (Sigma). Infectious viral titers were quantified by plaque assay in Vero-E6 cells in MEM supplemented with 2% FBS ...
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bioRxiv - Microbiology 2022Quote: ... Plates with Towne virus were stained with mouse anti-human CMV IE1 (MAB810, Millipore) then goat anti-mouse IgG-AF488 (Millipore) ...
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bioRxiv - Cancer Biology 2022Quote: ... thawed virus was mixed with culture media +8 μg/mL Polybrene (Millipore Sigma #107689) to infect cells overnight ...
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bioRxiv - Neuroscience 2021Quote: ... The pellet was dissolved in 0.5 ml virus lysis solution (50 mM TrisHCl; Sigma), 2 mM MgCl2 (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: ... virus-containing media were replaced by selection media containing 2 µg/ml puromycin (Sigma). All assays were performed 7–8 days after infection.
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bioRxiv - Microbiology 2021Quote: ... Virus were then buffer exchanged with Amicon Ultra-4 Centrifugal Unit (Millipore Sigma, #UFC800396) to get rid of furin inhibitor in the media.
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bioRxiv - Immunology 2020Quote: ... virus stocks were diluted at indicated MOIs in serum-free DMEM (D5756-500ML, Sigma). 300 μl serum-free media containing virus (or DMEM alone for the control wells ...
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bioRxiv - Cell Biology 2022Quote: ... medium containing the virus was collected and filtered with a 0.45 μm filter (Millipore). The filtered virus solution was directly used for the infection of cell lines or stored in aliquots at −80°C without prior concentration of the virus ...
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bioRxiv - Systems Biology 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5 mL aliquots in cryovials at -80ºC.
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bioRxiv - Systems Biology 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5 mL aliquots in cryovials at -80ºC.
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bioRxiv - Systems Biology 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5 mL aliquots in cryovials at -80ºC.
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bioRxiv - Systems Biology 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5 mL aliquots in cryovials at -80ºC.
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bioRxiv - Neuroscience 2023Quote: ... cells were lysed and virus was concentrated using an Optiprep density gradient (Sigma #D1556). Small-scale AAV virus was produced in HEK293T cells grown in 12-well plates by triple-transfection with 0.4μg HiUGE vector ...
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bioRxiv - Bioengineering 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5mL aliquots in cryovials at -80°C.
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bioRxiv - Microbiology 2023Quote: ... and resuspending in virus + polybrene (final conc 8 ug/mL, Sigma-Aldrich, TR-1003) or RPMI media + polybrene for the uninfected control at a concentration of 1E6 cells/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... the virus-containing supernatants from HEK293 cultures mixed with polybrene (Sigma, St. Louis, MO) at a final concentration of 8 mg mL-1 were added to the cells in each well ...
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bioRxiv - Bioengineering 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5mL aliquots in cryovials at -80°C.
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bioRxiv - Cancer Biology 2023Quote: ... Virus (400 µL) was added together with 5 ug/mL of polybrene (Sigma-Aldrich) and cells were centrifuged at 2,000 rpm for 2 h at 30°C ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lines were transduced with a mix of supernatant containing virus and polybrene (Sigma H9268 suspended at 4mg/ml in sterile water ...
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bioRxiv - Cell Biology 2024Quote: ... The virus was resuspended by fresh MEFs medium with 8 μg/ml polybrene (Sigma).
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bioRxiv - Genomics 2019Quote: ... We sequenced two biological and two technical replicates per isolate and used antibodies against the euchromatin histone mark H3K4me2 (#07–030, Merck Millipore). Sequencing was performed at the OSU Center for Genome Research and Biocomputing (Oregon State University ...
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bioRxiv - Microbiology 2021Quote: ... FQ-R isolates were selected by plating processed samples onto Tryptone Bile X-Glucuronide (TBX) agar (Sigma-Aldrich, Dorset, UK) containing 0.5 mg/L ciprofloxacin ...
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bioRxiv - Microbiology 2020Quote: The Congo red binding (CRB) ability of the APEC isolates was determined using agar plates supplemented with 0.003% CR dye (Sigma, USA) and 0.15% bile salts ...
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bioRxiv - Microbiology 2020Quote: ... Pneumococcal isolates were grown in triplicate in BHI in 96-well plates in the presence of hydrogen peroxide (H202, Sigma) at concentrations 0 ...
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bioRxiv - Microbiology 2021Quote: ... was used to test isolates for growth on xanthan oligosaccharides. Some isolates (e.g. Parabacteroides distasonis) required the inclusion of 5 mg/mL beef extract (Sigma, B4888) to achieve robust growth on simple monosaccharides ...
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Protective microbe enhances colonisation of a novel host species by modifying immune gene expressionbioRxiv - Microbiology 2019Quote: ... We plated serial dilutions onto Mannitol Salt Agar to isolate S.aureus and TSB with 100 μg/ml rifampicin (Sigma-Aldrich) to isolate E ...
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bioRxiv - Microbiology 2022Quote: ... the collected supernatants of each isolate were concentrated using a Centricon Plus-70 centrifugal filter device (100 K nominal molecular weight limit; Millipore) resulting in an approximately 100x concentrated stock (v/v ...
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bioRxiv - Pathology 2020Quote: ... a full-length P1 nucleotide sequence originated from the RYMV Tz3 isolate was already cloned in a pET3b vector (Novagen) between NdeI and BamHI restriction sites to produce the P1 referent protein (Gillet et al ...
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bioRxiv - Microbiology 2023Quote: ... The strains and isolates were revived by culturing from frozen stock on Sabouraud dextrose agar (SDA) plates (Millipore, Dramstadt, Germany) and incubated at 35°C for 48h ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were selected with hygromycin at a final concentration of 100 µg/ml and plated at low density to isolate single clones and screened via Western Blot for endogenous KDM3A or KDM3B and FLAG (M2 FLAG Sigma). FLAG-tagged protein expression was induced with 1ug/ml doxycycline.
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bioRxiv - Genomics 2020Quote: ... An amount of 35 or 40 μg whole cell lysates or tissue lysates were resolved by 10% SDS-PAGE and transferred onto PVDF membranes (Merck Millipore, Germany). After incubation in 5% non-fat milk for 1 h at room temperature ...
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bioRxiv - Systems Biology 2022Quote: Caco-2 cell lysates expressing FLAG-KRAS proteins were immunoprecipitated from 800 μg of cell lysate using anti-FLAG-M2 magnetic beads (Sigma, M8823) by using the KingFisher DuoPrime purification system (ThermoFisher) ...
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bioRxiv - Cancer Biology 2021Quote: 500 µl diluted lysate was incubated with the antibody against β-catenin (10 µg; Sigma, MA1-301, 1:2000) overnight at 4L°C with gentle rocking ...
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bioRxiv - Bioengineering 2022Quote: ... FDG is mixed in LB to 0.2 mM with IPTG (1 mM, final concentration), sodium N-lauroyl sarcosine solution (0.1%, cell lysate buffer to allow FDG in) (Sigma), Pluronic F127 (0.001%) ...
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bioRxiv - Biochemistry 2021Quote: ... and the lysate was incubated for 1 h with a previously equilibrated His-Select Nickel Affinity Gel (Sigma-Aldrich). Weakly-bound proteins were removed using buffer A (20 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mg of clarified lysate was incubated with 30 μl of pre-rinsed streptavidin magnetic particles (11641786001, Sigma-Aldrich) in 1 ml RIPA buffer for 16h ...
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bioRxiv - Immunology 2021Quote: ... 1 mM EDTA) buffer and OAS1 proteins were immunoprecipitated from the lysate using 20 µg anti-FLAG antibody (Sigma) and Dynabeads Protein G ...
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bioRxiv - Microbiology 2023Quote: ... THP-1 cells (100% confluent) were harvested and lysates prepared using CytoBuster protein extraction reagent (Novagen/EMD, Gibbstown, NJ) supplemented with complete mini EDTA-free protease inhibitor (Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... Chromatin lysates were prepared in LB3 (50 mM Tris-HCl pH8.0, 1% SDS, 10 mM EDTA, proteinase inhibitor cocktail (Sigma)) ...
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bioRxiv - Microbiology 2024Quote: ... HA tagged proteins were detected in cell lysates using mouse monoclonal anti-HA antibody (Cat# H9658, Sigma, 1:10,000). HA tagged proteins were detected in virus lysates using rabbit polyclonal anti-HA antibody (Cat# H6908 ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 ml of the lysate was added to 40 μl of equilibrated ANTI-FLAG® M2 Affinity Gel (Sigma). The lysate was incubated with the affinity gel in the cyclomixer for 4 hours at 4 ⁰C ...
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bioRxiv - Genomics 2020Quote: ... Single IMR90 isolates (I90-10 and −79, passage 5; Coriell Biorepository) were continuously passaged to replicative exhaustion in MEM (M4655, Sigma-Aldrich) supplemented with non-essential amino acids and 10% FBS under 5% CO2 ...
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bioRxiv - Neuroscience 2022Quote: ... then to 0 mV to isolate inhibitory currents which got pharmacologically blocked by bath application GABAA antagonist Gabazine (10uM; Sigma-Aldrich). Then cells were hyperpolarized to -80 mV to isolate AMPA currents which were then were blocked by NBQX (20uM ...
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bioRxiv - Microbiology 2019Quote: ... Cultures of each isolate were plated on Mueller-Hinton agar with 1mL of a 50mg/mL sodium phosphonoformate (PPF; Sigma-Aldrich) solution to a 200 µg fosfomycin disk supplemented with 50µg G6P ...
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bioRxiv - Microbiology 2019Quote: ... PCR was used to detect the mecA gene.[9] Isolates with zone sizes less than 21 mm for cefoxitin discs (Sigma, USA), according to the criteria of Clinical and Laboratory Standards Institute (CLSI),[10] and which were also mecA gene positive were considered MRSA ...