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Citations for Millipore Sigma :
201 - 250 of 654 citations for Lactose operon repressor LacI E.coli His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... and extensively dialysed against HBS and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag monoclonal antibody, 70796, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (A3562 ...
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bioRxiv - Immunology 2023Quote: ... All antibodies were incubated separately at a concentration of 10 μg/ml in tubes together with 330 ng/ml HIS-tagged CD40 recombinant protein and 4 μg/ml peroxidase-coupled anti-HIS detection antibody (Sigma-Aldrich) for 60 minutes in ELISA buffer (PBS ...
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bioRxiv - Biophysics 2020Quote: ... the soluble His-tagged proteins were produced and purified after binding to a Ni-NTA His-Bind resin (Sigma-Aldrich, MO, US) as described by Martínez-Martínez (19) ...
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bioRxiv - Biophysics 2020Quote: TCRβ-reactive H57 antibody single chain fragments featuring a C-terminal unpaired cysteine residue (H57 cys scFV) were expressed in E.coli BL-21 as inclusion bodies using the pET expression system (Novagen) and refolded as described (9 ...
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bioRxiv - Biophysics 2020Quote: ... biotin binding deficient subunits equipped with a C-terminal His6-tag – “dead” – and functional subunits with C-terminal 3C protease cleaving site followed by Glu6-tag – “alive” – were expressed as inclusion bodies in E.coli BL-21 using the pET expression system (Novagen). Inclusion bodies were dissolved in 6 M Guanidine hydrochloride at 10 mg/ml ...
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bioRxiv - Immunology 2021Quote: ... The gene sequence was amplified without the signal sequence and then PCR amplicons were ligated into the E.coli expression vector pET28a (Novagen) at the restriction sites shown in Table 1 ...
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bioRxiv - Systems Biology 2020Quote: Recombinant HA-tagged Gln4 protein for use as a standard was produced by transforming E.coli strain Rosetta-gami B (DE3; Novagen) with pET19b-GLN4 ...
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bioRxiv - Immunology 2021Quote: Ccl19-Cre x R26R-EYFP mice were subcutaneously injected in one flank with 5 mg of LPS from E.coli (Sigma) together with 100 mg Ovalbumin grade VI (Sigma) ...
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bioRxiv - Biochemistry 2020Quote: ... or catalytically-inactive BT4656S1_11 (in which Ser 77 is intact but prevents formation of formylglycine during expression in E.coli) were expressed in Escherichia coli strain TUNER (Novagen), and cultured to mid-exponential phase in LB supplemented with 50 μg/mL kanamycin at 37 °C and 180 rpm ...
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bioRxiv - Cell Biology 2021Quote: ... for 5min and then with hybridization buffer (10% Dextran sulfate Sigma D8906, 20% Formamide, 1mg/ml E.coli tRNA Sigma R1753 ...
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bioRxiv - Cell Biology 2021Quote: ... the tissues were mounted with the hybridization buffer (10% Dextran sulfate Sigma D8906, 20% Formamide, 1 mg/ml E.coli tRNA, Sigma R1753 ...
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bioRxiv - Cell Biology 2020Quote: ... for 5 min and mounted with the hybridization buffer (10% Dextran sulfate Sigma D8906, 20% Formamide, 1 mg/ml E.coli tRNA Sigma R1753 ...
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bioRxiv - Immunology 2020Quote: ... a 5mg/kg nonlethal dose and 35mg/kg lethal dose of gram negative bacterial lipopolysaccharide (E.coli O55:B5, Sigma) was injected intraperitoneally ...
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bioRxiv - Developmental Biology 2021Quote: ... and mounted with the hybridization buffer (10% Dextran sulfate Sigma D8906, 30% Formamide, 1 mg/ml E.coli tRNA Sigma R1753 ...
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bioRxiv - Physiology 2020Quote: ... or intravenous E.coli LPS (1 μg/kg infused over one hour; O55:B5, Sigma-Aldrich, Castle Hill, NSW, Australia). Once a PaO2/FiO2 ratio <100 mmHg (PEEP ≥5 cmH2O ...
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bioRxiv - Plant Biology 2019Quote: ... a C-terminal polypeptide encompassing the last 284 amino acids of the PKL protein has been expressed in fusion with a hexahistidine tag in the E.coli strain BL21DE3 Rosetta (Novagen). The protein was purified under denaturing conditions on Ni2+-nitrilotriacetic acid matrix (Ni-NTA Agarose ...
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bioRxiv - Biochemistry 2019Quote: ... A synthetic gene encoding the KRAB domain (residues 1-71) from ZNF93 (UniProt: P35789) codon-optimized for E.coli was expressed from the pET20 plasmid (Novagen) with N-terminal Twin-StrepII and maltose binding protein (MBP ...
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bioRxiv - Cell Biology 2019Quote: ... were injected iv with purified HPSE2 (5μg/g body weight) and immediately thereafter low dose (5 ng/g body weight) of LPS (E.coli O111:B4, Sigma) administration was performed by iv injection ...
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bioRxiv - Immunology 2020Quote: SPF and GF C57Bl/6Jrj mice were anesthetized with ketamine/xylazine (Rompun/Imalgene) and 10 ng/g LPS from E.coli O111: B4 (Sigma) or saline solution (PBS ...
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bioRxiv - Biochemistry 2023Quote: ... Y730F)41 was cloned into pET28a vector incorporating a His6-tag and a SUMO tag at the amino terminus of the expressed protein and expressed in E.coli BL21 gold strain (Novagen). Cells were grown at 37°C in TB medium containing 0.1 mg/ml kanamycin and 0.050 mg/ml tetracyline to an OD600 of ∼3.0 ...
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bioRxiv - Biophysics 2020Quote: ... The (His)8-tag was cleaved by thrombin (Sigma) using 20 U per 1 mg GltPh in the presence of 5 mM CaCl2 at room temperature overnight ...
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bioRxiv - Biophysics 2021Quote: ... 6×His-tagged recombinant MSP1E3D1 (Sigma-Aldrich, Merck, USA) and FLAG-tagged recombinant mCardinal (kindly provided by Jakub Czapiński ...
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bioRxiv - Biochemistry 2020Quote: ... and quantitative immunoblotting using an anti-His antibody (Novagen).
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bioRxiv - Cell Biology 2022Quote: ... mouse α-His-HRP (A7058, Sigma-Aldrich, 1:10000).
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bioRxiv - Plant Biology 2019Quote: ... and anti-His (Sigma-Aldrich, St. Louis, MO, USA) antibodies.
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bioRxiv - Biochemistry 2021Quote: ... recombinant His-Akt1 was from EMD Millipore (#14-279); recombinant GST-mTORf (containing a 30 fragment of mTOR encoding amino acids 2144-2175 ...
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bioRxiv - Biophysics 2022Quote: ... Anti-His mAb was from Sigma (St. Louis, MO). Anti-A1 mAb CR1 was a generous gift from M ...
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bioRxiv - Biochemistry 2023Quote: ... His tag and Flag tag (1:1000; F3165, Sigma).
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bioRxiv - Biochemistry 2020Quote: ... The production of the (His)6-tagged recombinant proteins was confirmed by Western analysis using mouse monoclonal α-His-horseradish peroxidase conjugated antibodies (Sigma Aldrich, USA). Imaging of the protein bands on the Western blot was conducted using the ECL kit (ThermoScientific ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 ug RNA was mixed with 0.025 pmole of tRNA standards (e.coli-tyr-tRNA and s.cerevisia-phe-tRNA at ration of 1:8 (Sigma-Aldrich; XX)) ...
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bioRxiv - Cell Biology 2022Quote: ... non-adherent cells were collected and DC maturation was induced by overnight incubation with 200ng/ml of lipopolysaccharide (LPS) from E.coli 0127:B8 (Sigma, L4516). Before every experiment ...
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bioRxiv - Biochemistry 2020Quote: ... The recombinant proteins were overexpressed in E.coli BL21 (DE3) at 17 °C overnight and then purified by Glutathione-agarose (Sigma #G4510). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... coli O111:B4 (catalog no. L3024) and lipid A from E.coli F583 (Rd mutant, catalog no. L5399) were purchased from Sigma Aldrich. Deuterium oxide was from Cambridge Isotope Laboratories (Tewksbury ...
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bioRxiv - Cell Biology 2020Quote: ... the codon optimized gene encoding for rPMDs was subcloned into PET28a (+) and protein expression in E.coli cells was induced with 1 mM isopropyl-β-D-thiogalactoside (IPTG) (Sigma). The his-tagged proteins were purified using Ni-NTA chromatography and the concentration was determined using the BCA estimation kit (Pierce).
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bioRxiv - Neuroscience 2020Quote: ... were expressed as fusion proteins with 6x polyHis tail at the C-terminus in E.coli strain BL21(DE3) (Merck-Novagen, Darmstadt). After harvesting ...
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bioRxiv - Biochemistry 2019Quote: MBP-Ypt7-tm was purified as follows: MBP-Ypt7-tm was produced in E.coli Rosetta(DE3)pLysS (Novagen, Milwaukee WI). A single colony was inoculated into 50ml LB medium containing 100 μg/ml ampicillin (Amp ...
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bioRxiv - Immunology 2021Quote: ... and 1 ml of fresh media (IMDM+10%FBS+1%A/A) was added with either 100 ng/ml LPS (E.coli; Serotype 0111:B4; Sigma Aldrich) or control for 24h ...
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bioRxiv - Immunology 2019Quote: ... For survival experiments and flow cytometry analysis of peritoneal lavage fluid cells mice received 15 mg/kg LPS (E.coli O55:B5; purified by phenol extraction; Sigma-Aldrich) one week after heme treatment ...
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bioRxiv - Neuroscience 2024Quote: ... Media was removed and cells were subsequently incubated in 160µl E.coli solution containing either 5µM Cytochalasin D (Sigma-Aldrich, Cat#: C8273) as a negative control for phagocytosis ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were cultured and passaged every 2 or 3 days in Dulbecco’s modified Eagle’s medium (DMEM) (Hi Media Laboratories) supplemented with 10% FBS (Hi Media Laboratories) and 1% penicillin/streptomycin (Sigma-Aldrich, St. Louis, MO, USA). Both cell types were cultured at 37°C and in a humidified incubator with a 5% CO2 atmosphere ...
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bioRxiv - Bioengineering 2019Quote: The His-TEV tag was cleaved using TEV protease (Sigma). The reaction was made overnight at 4°C into dialysis buffer for TEV (25 mM Tris-HCl ...
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bioRxiv - Plant Biology 2019Quote: ... HIS-TAN1-GFP was conjugated with Atto488-maleimide (Sigma 28562). 4μM HIS-TAN1-GFP in 80mM PIPES ...
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bioRxiv - Immunology 2021Quote: ... This was followed by 0.5ug/mL anti-his HRP (Sigma), and binding was detected after incubation with substrate at an absorbance of 450nm ...
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bioRxiv - Biochemistry 2020Quote: ... The antibodies used for western blotting were anti-His (Sigma), anti-Strep HRP conjugated (iba ...
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bioRxiv - Biochemistry 2020Quote: ... FLAG tag (F1804) or His tag (H1029) were from Sigma. Mouse monoclonal antibody raised against hemagglutinin (HA ...
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bioRxiv - Plant Biology 2020Quote: ... and 1:5000 dilution of primary anti-His (SIGMA, USA), 1:10000 for anti-GST (SIGMA ...
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bioRxiv - Molecular Biology 2021Quote: ... SD-Leu-Trp-His+1mM 3-AT (3-aminotriazol, Sigma) and SD-Leu-Trp-His-Ade (Formedium).
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bioRxiv - Cancer Biology 2020Quote: ... 100 ng recombinant His-AKT1 (inactive, Upstate/Millipore #14-279), at a final volume of 30 μl ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were detected using α -His from mouse (Sigma H1029), α-Gfp from mouse ...
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bioRxiv - Biochemistry 2020Quote: Recombinant Ded1-His was expressed from the pET22b plasmid (Novagen) and purified as previously described (46) ...