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Citations for Millipore Sigma :
1 - 50 of 654 citations for Lactose operon repressor LacI E.coli His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... and α-LacI (Millipore, 05-503).
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-LacI (1:1000; Millipore). We used Alexa fluor secondary antibodies (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2020Quote: ... 1 mM acceptor (lactose (Sigma) and lacto-N-neotetraose (LNnT ...
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bioRxiv - Biochemistry 2022Quote: ... and lactose (Sigma Aldrich Japan) were utilized as hapten sugar to ConA and SSA ...
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bioRxiv - Microbiology 2023Quote: ... 1 mM sialyl-lactose (Sigma) containing 0.2% BSA ...
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bioRxiv - Biochemistry 2021Quote: ... pNP-lactose (pNP-Lac; Sigma-Aldrich), pNP-xylopyranoside (pNP-Xylp ...
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bioRxiv - Cell Biology 2023Quote: ... β-D-lactose (Sigma, Ref. L3750), NHS-Cy3 (GE ...
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bioRxiv - Cancer Biology 2021Quote: ... β-Lactose (Sigma-Aldrich, St. Louis, MO) was included as a positive control for galectin-3 binding inhibition ...
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bioRxiv - Microbiology 2022Quote: ... Lactose (L3750) was obtained from Sigma Aldrich, Maltose (M2250 ...
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bioRxiv - Immunology 2023Quote: A solution of lactose purchased from Sigma‒Aldrich at 100 mM was used to prepare working standard solutions of 100 ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse (Ms)-anti-LacI (1:250, Millipore 05-503), Rb-anti-H3K9ac (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse (Ms)-anti-LacI (1:250, Millipore 05-503), was performed at 4°C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... Solutions of α-Lactose monohydrate (Sigma, L2643-500), sucrose (Fisher ...
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bioRxiv - Bioengineering 2021Quote: ... Dynasore and Lactose were ordered from Sigma Aldrich. Nuclease free water ...
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bioRxiv - Neuroscience 2022Quote: ... A lactose assay kit (Sigma-Aldrich Cat. #MAK017) was used to measure lactose content in milk diluted 1:500 with lactose assay buffer (as outlined by Chen et al. ...
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bioRxiv - Genetics 2020Quote: ... 0.2% lactose (Sigma Aldrich, St. Louis, MO, USA), 0.001% thiamine (Sigma Aldrich) ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 2% (v/v) lactose (Sigma-Aldrich) and 20 μg/ml of thymidine (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... E.coli cells (Novagen) with pNIC-CH vectors was prepared via transformation ...
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bioRxiv - Bioengineering 2021Quote: ... packed with α-lactose agarose affinity resin (Sigma-Aldrich). Proteins were eluted with a linear gradient of β-lactose (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 0.4% w/v lactose (Sigma-Aldrich, L3750). Individual colonies were randomly chosen and picked with a sterile stick and simultaneously streaked onto an EMB plate and a DM plate ...
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bioRxiv - Bioengineering 2023Quote: ... 0.2 % D-Lactose (Sigma Aldrich; CAS 64044-51-5) and 0.05 % D-Glucose monohydrate (Thermo Scientific Chemicals ...
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bioRxiv - Biochemistry 2019Quote: ... The heterodimeric SMR transporters operons were expressed from pET19b (Novagen) as described19.
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bioRxiv - Molecular Biology 2021Quote: ... un-spliced precursor and mutant SufB proteins carrying an N-terminal 6X-His-tag were over-expressed in BL21 (DE3) E.coli cells via IPTG (500µM) (Sigma 367-93-1) induction at 37°C for 4hrs ...
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bioRxiv - Immunology 2019Quote: E.coli strain BL21DE3(pLysS) (Novagen) was transformed with RNF144A constructs cloned into PGEX-5X3 to allow production of GST/RNF144A fusion proteins ...
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bioRxiv - Immunology 2020Quote: ... LPS E.coli 0111:B4 (Sigma) and poly(I:C ...
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CRISPR-Csx28 forms a Cas13b-activated membrane pore required for robust CRISPR-Cas adaptive immunitybioRxiv - Biochemistry 2021Quote: ... E.coli Rosetta2 (DE3) pLysS (Novagen) transformants were grown in terrific broth (TB ...
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bioRxiv - Neuroscience 2023Quote: ... 1mg/ml E.coli tRNA (Sigma), 1mg/ml salmon sperm DNA (Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... E.coli BL21(DE3) (Merck Millipore) cells were grown at 22°C in LB autoinduction media (AIMLB0210 ...
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bioRxiv - Bioengineering 2021Quote: ... Supernatant containing soluble recombinant protein was loaded into α-lactose agarose (Sigma) affinity column preequilibrated with PBS ...
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bioRxiv - Bioengineering 2021Quote: ... Proteins were eluted with a linear gradient of β-lactose (Sigma-Aldrich) in phosphate buffer.
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bioRxiv - Neuroscience 2022Quote: ... commercially available ELISA kits were used to measure lactose content (Sigma-Aldrich Cat ...
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bioRxiv - Genetics 2020Quote: Cells were streaked on LB agar with 0.1% Lactose (Sigma L-3625), 40μg/ml X-Gal (Sigma B4252 ...
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bioRxiv - Synthetic Biology 2023Quote: ... lac operon amplicon was amplified from the pETDuet-1 plasmid (Novagen 71146) and cloned into the pBBR1-MCS1 plasmid to construct the pBBR1-MCS1-plac plasmid ...
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bioRxiv - Plant Biology 2020Quote: E.coli strains Rosetta pLysS (Novagen, USA) and T7 Shuffle (New England Biolabs ...
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bioRxiv - Immunology 2022Quote: ... E.coli LPS-055:B5 (Sigma, L2880) was injected at a dose of 1mg/kg body weight diluted in sterile low endotoxin PBS (Armitage et al. ...
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bioRxiv - Cell Biology 2023Quote: Rosetta 2(DE3) E.coli cells (Novagen) were transformed with the plasmids expressing His6-SUMO3 tagged KSR probes or GFP alone (control) ...
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bioRxiv - Synthetic Biology 2021Quote: ... Western blot analysis was performed using a monoclonal antibody against LacI (Sigma-Aldrich #05-503-I) and an anti-mouse HRP-conjugated secondary antibody (Invitrogen #31430) ...
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bioRxiv - Cell Biology 2019Quote: ... 1 mg/ml E.coli tRNA (Sigma, R8759), 2 mM ribonucleoside vanadyl complex (RVC ...
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bioRxiv - Microbiology 2021Quote: ... E.coli BL21 (DE3) from Novagen (Shanghai, China) was used to express recombinant enzyme ...
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bioRxiv - Plant Biology 2023Quote: ... protein expression in E.coli Rosetta cells (Novagen) was induced with 0.5 mM IPTG (isopropyl-β-D-thiogalactopyranoside ...
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bioRxiv - Microbiology 2020Quote: ... pH 6.5 and lactose was digested with β-galactosidase from Kluyvermomyces lactis (Sigma Aldrich) (20 U mL-1 ...
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bioRxiv - Physiology 2021Quote: The first step is based on affinity chromatography on agarose-lactose columns (Sigma-Aldrich), previously equilibrated with equilibration buffer (1XPBS ...
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bioRxiv - Cell Biology 2022Quote: ... or by using BL21 (DE3) E.coli cells (Sigma) and expression in LB medium at +l6°C for 30 hours ...
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bioRxiv - Biochemistry 2021Quote: ... Competent E.coli BL21(DE3) PLysS cells (Novagen #69451) (50 μL ...
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bioRxiv - Biophysics 2023Quote: ... Proteins were overexpressed in E.coli Rosetta (DE3) (Novagen) cells and cultured in LB broth to an OD600 of 0.6-0.8 ...
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bioRxiv - Biochemistry 2024Quote: ... wildtype K12 E.coli were treated with actinonin (Sigma) at a final concentration of 60 µg/mL when OD600 reached 1.0 and incubated for 0 ...
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bioRxiv - Cell Biology 2022Quote: ... α-Lactose-Agarose beads used for galectin-8 purification were purchased from Sigma (Catalog no. L7634). The glass-bottomed petri-dishes (MatTek ...
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bioRxiv - Cell Biology 2020Quote: ... α-Lactose-Agarose beads used for galectin-8 purification were purchased from Sigma (Catalog no. L7634). Galectin-8 mutated forms were generated as previously described (Levy et al. ...
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bioRxiv - Cell Biology 2024Quote: ... respectively (Millipore Cat# HI-14K, HI-13K). Samples treated with 3 mM glucose KRBH were measured undiluted ...
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bioRxiv - Cell Biology 2020Quote: ... were transformed into the Rosetta2 strain of E.coli (Novagen). 1-4 liters of culture were grown in terrific broth for 8-12h at 37°C (until the OD600 ∼2-3) ...