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Citations for Millipore Sigma :
51 - 100 of 654 citations for Lactose operon repressor LacI E.coli His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... E.coli 0111:B4 LPS purified by gel filtration (Sigma), R848 (Invivogen) ...
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bioRxiv - Physiology 2022Quote: ... with 100μg LPS endotoxin (E.coli O111:B4, Sigma-Aldrich) and culled 2 hours later for collection of serum and peritoneal fluid (the latter obtained by lavaging the peritoneal cavity of euthanized mice with 1mL PBS) ...
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bioRxiv - Immunology 2020Quote: ... LPS (500 ng/mL, E.coli 011:B4, Sigma-Aldrich), Imiquimod (10 μg/mL ...
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bioRxiv - Immunology 2022Quote: PhyB was produced in BL21(DE3) E.coli (Sigma Aldrich) transfected with three plasmids obtained from the laboratory of Dr K Rosen and Leung (Leung et al. ...
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bioRxiv - Neuroscience 2022Quote: ... E.coli cells were lysed by using BugBuster Kit (Novagen) and purified with Glutathioine Sepharose 4B (GE healthcare ...
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bioRxiv - Plant Biology 2023Quote: ... Plasmids were transformed into E.coli Rosetta2 (DE3) cells (Novagen). Bacteria were grown in LB medium at 37 °C up to an OD600nm of 0.6 ...
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bioRxiv - Biochemistry 2023Quote: ... were expressed in Rosetta(DE3)pLysS E.coli strain (Millipore). Briefly ...
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bioRxiv - Biochemistry 2021Quote: ... the whole operon of FhuCDB was cloned from Escherichia coli (E. coli) genome into a pET15b (Novagen) vector with a His-tag and a thrombin digestion site on the N-termini of FhuC ...
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bioRxiv - Biochemistry 2020Quote: ... the E.coli cells of Origami™ B(DE3) pLysS (Novagen) transformed with the pLW01 vector were grown at 37 °C in lysogeny broth medium until OD600 ~ 0.6 ...
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bioRxiv - Biochemistry 2022Quote: SPOC domains were expressed in E.coli Rosetta2 (DE3) cells (Novagen). O/N cultures were diluted 1:50 in 2xTY ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein was expressed E.coli strain Rosetta 2 (DE3) (Novagen). The cells were grown at 37 °C in Luria-Bertani medium (LB ...
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bioRxiv - Biophysics 2023Quote: ... All recombinant plasmids were transformed into E.coli Rosetta (DE3) (Novagen) cells and grown in LB broth at 37°C for 3 h ...
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bioRxiv - Molecular Biology 2024Quote: ... hsZEB1 (28-45) were expressed in E.coli Rosetta (Sigma Aldrich). Cells were grown at 37 °C in LB medium with appropriate antibiotics until OD600 = 0.5 was reached ...
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bioRxiv - Molecular Biology 2019Quote: ... His-GFP or His-fusion proteins were bound to HIS-Selec HF Nickel Affinity Gel (Sigma) for 1 hour ...
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bioRxiv - Immunology 2019Quote: ... the total soluble tachyzoite antigen (STAg) fraction was loaded into a lactose column (Sigma-Aldrich, St. Louis, MO) and equilibrated with PBS containing 0.5 M NaCl ...
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bioRxiv - Cell Biology 2019Quote: ... α-His (Sigma H1029). α-Rabbit IgG HRP conjugate (Promega W4011) ...
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bioRxiv - Immunology 2021Quote: ... held upright and intranasally challenged with LPS (E.coli O111:B4; Sigma) at a concentration of 2.5 μg per gram of mouse in 0.9% saline (100 μl) ...
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bioRxiv - Plant Biology 2020Quote: ... 5 μg of purified His fusion protein (PuBZR1-His) was bound to Ni-NTA His binding resin (Novagen). GST fusion proteins containing PuACO1 (PuACO1-GST ...
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bioRxiv - Microbiology 2022Quote: ... SasG-His was then re-purified using HIS-Select resin (Sigma) and eluted with bind buffer containing increasing concentrations of imidazole ...
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bioRxiv - Systems Biology 2021Quote: ... separated proteins were transferred to a PVDF membrane using a semi-dry system and His-tagged proteins (hCDKL5 and His-Neuropilin) were detected with a HRP-conjugated anti-His antibody (1:2,000, Sigma) using the Enhanced Chemiluminescence kit (ECL ...
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bioRxiv - Microbiology 2021Quote: We used TSA plates containing 5% lactose-free, skim milk (Fairlife, LLC) or 0.5% Type II porcine gastric mucin (Sigma Aldrich) to screen the otic isolates for mucinase and protease secretion ...
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bioRxiv - Bioengineering 2021Quote: ... Columns were washed with 20 column volumes PBS and recombinant proteins were eluted with 100 mM β-d-lactose (Sigma) prepared in PBS ...
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bioRxiv - Microbiology 2019Quote: ... The pH was adjusted to 6.8 in both media and filter sterilized carbohydrate source supplementation (glucose, lactose, galactose, inulin, starch or pectin [Sigma, UK]) was added at 2% after autoclaving ...
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bioRxiv - Microbiology 2020Quote: ... The transformants were then plated onto MacConkey lactose agar containing 100 μg/ml ampicillin and 100 mM of iron sulfate (FeSO4) (Sigma). After 18 h of incubation at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Thereafter 10 μl of the culture was streaked on SA (1% lactose or glucose) agar plate supplemented with 10 μg/ml 5-fluoroorotate (Sigma). Plates were incubated at 30°C until 5-fluoroorotate resistant colonies emerged ...
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bioRxiv - Cell Biology 2020Quote: ... These plasmids were co-transformed into the Rosetta2 strain of E.coli (Novagen). Multiple liters of culture were grown in terrific broth for 8-12h at 37°C (until the OD600 ∼2-3) ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... and transformed into E.coli SIG10 5-α Chemically Competent Cells (Sigma-Aldrich). Positive clones were selected using LB broth agar plates with 100 µg/ml ampicillin ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 48 hours before immune challenge with LPS (E.coli O111:B4, Sigma-Aldrich) administered either i.n ...
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bioRxiv - Biochemistry 2020Quote: ... the protein was overexpressed in Rosetta (DE3) pLysS E.coli cells (Millipore Sigma) and grown in Terrific Broth (TB ...
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bioRxiv - Synthetic Biology 2022Quote: ... The assembled DNA-of-interest was inserted into a pET22b expression vector containing a T7 promoter and lac operon (Novagen). Ligated plasmids were electroporated into E ...
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bioRxiv - Biochemistry 2020Quote: ... HIS (Sigma-Aldrich H3667), purified full-length Vn (1 μM in PBS ...
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bioRxiv - Microbiology 2021Quote: ... Anti-His (Millipore, USA), anti-GST (Sigma ...
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bioRxiv - Plant Biology 2020Quote: ... or anti-His (Novagen) antibodies to detect Ub conjugates.
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bioRxiv - Plant Biology 2020Quote: ... and anti-His (Sigma) antibodies.
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bioRxiv - Plant Biology 2020Quote: ... and anti-His (Sigma) antibodies at a 1:2000 dilution ...
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bioRxiv - Molecular Biology 2019Quote: ... anti-His (Sigma, H1029) 1:2000 ...
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bioRxiv - Plant Biology 2022Quote: ... anti-His (Sigma H1029), anti-GFP (Agrisera ...
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bioRxiv - Molecular Biology 2020Quote: ... cleaved His-tag and His-tag-fused TEV protease were removed using a HIS-Select® Nickel Affinity Gel (Sigma-Aldrich) column ...
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bioRxiv - Biochemistry 2024Quote: ... the induction was done either by 1% (w/v) lactose or 0.5 mM isopropyl-β-D-1-thio-galactopyranoside (IPTG, Sigma Aldrich) (Table 1) ...
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bioRxiv - Microbiology 2019Quote: ... Bound His-tagged proteins were detected using HRP-conjugated anti-His antibodies (Sigma) at a 1:500 dilution ...
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bioRxiv - Microbiology 2020Quote: ... coli BL21(DE3)[F– ompT gal dcm lon hsdSB(rB-mB-) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5] (Novagen, Madison, WI, USA) was used as a host strain for overproduction of a His-tagged form of FklB or Y181A ...
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bioRxiv - Biophysics 2021Quote: All TwcK sample variants were expressed in E.coli Rosetta (DE3) cells (Merck Millipore) and grown in LB media containing 25 µg/mL kanamycin and 34 µg/mL chloramphenicol ...
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bioRxiv - Biochemistry 2021Quote: ... and/or LPS (1 µg, E.Coli 0111.B4, Sigma-Aldrich, Cat. No L3024) or vehicle (PBS ...
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bioRxiv - Biophysics 2020Quote: ... The CbAgo protein was expressed in E.coli Bl21(DE3) Rosetta™ 2 (Novagen). Cultures were grown at 37 °C in LB medium containing 50 μg ml−1 kanamycin and 34 μg ml−1 chloramphenicol till an OD600 nm of 0.7 was reached ...
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bioRxiv - Cell Biology 2022Quote: E.coli (DH5 alpha) were cultured overnight at 37°C in Luria broth (Sigma), washed in PBS and then fixed in 4% paraformaldehyde for 20 mins at room temperature ...
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bioRxiv - Biophysics 2019Quote: ... The CbAgo protein was expressed in E.coli Bl21(DE3) Rosetta™ 2 (Novagen). Cultures were grown at 37°C in LB medium containing 50μa.g ml-1 kanamycin and 34μg ml-1 chloramphenicol till an ODóOOnm of 0.7 was reached ...
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bioRxiv - Molecular Biology 2019Quote: ... or opFAs and lipopolysaccharide (LPS) (100 ng/ml) (LPS from E.coli) (Sigma Aldrich) in Williams E Medium (William’s Medium E ...
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bioRxiv - Cell Biology 2019Quote: ... cells were stimulated overnight with 200ng/ml Lipopolysaccharide from E.coli 0127:B8 (Sigma) and used for experiments on days 9-10.
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bioRxiv - Immunology 2020Quote: ... pGEN SpyCatcher AP205-CP3 was transformed into OverExpress™ C41(DE3) E.coli (Sigma). Single colonies were picked and inoculated into a 2xYT (Sigma ...
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bioRxiv - Cell Biology 2019Quote: All proteins were expressed and purified from E.coli Rosetta (DE3) Competent Cells (Novagen). Details of individual constructs and their expression plasmids are detailed in the plasmids section ...