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Citations for Millipore Sigma :
151 - 200 of 654 citations for Lactose operon repressor LacI E.coli His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... cOmplete His-Tag Purification Resin (Sigma Millipore) was applied for affinity purification ...
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bioRxiv - Immunology 2020Quote: ... cOmplete His-Tag Purification Resin (Sigma Millipore) was applied for affinity purification ...
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bioRxiv - Plant Biology 2020Quote: ... and purified on His•Bind resin (Novagen) in the presence of 8 M urea according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... anti-His monoclonal antibody (Sigma, 1:1000) and anti-mouse (Sigma ...
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bioRxiv - Neuroscience 2019Quote: ... was incubated with His-HDAC6 (EMD Millipore) and 1 mM ATP in kinase buffer for 1 h at 37°C ...
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bioRxiv - Immunology 2020Quote: ... then detected with anti-His antibody (Sigma). S protein expression was detected with anti-SARS-CoV-2 S protein S2 subunit mouse mAb unconjugated (GeneTex) ...
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bioRxiv - Immunology 2020Quote: ... with added 10% HI FBS (Sigma-Aldrich) and 1% Pen/Strep (Lonza) ...
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bioRxiv - Cell Biology 2021Quote: ... or anti-HIS (1:10,000, Sigma H1029) was used ...
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bioRxiv - Neuroscience 2021Quote: ... amino acids (His, Ser, Ala, Trp; Sigma) were diluted to a final concentration of 10-4 M and bile acid (TDCA ...
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bioRxiv - Microbiology 2023Quote: ... anti-His (mouse, 1:3k, Sigma, A7058), anti-c-Myc (mouse ...
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bioRxiv - Biochemistry 2022Quote: ... anti-His (Millipore: 05-949, clone HIS.H8) or Goat anti-Mouse IgG (Millipore ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and His-Bind Purification kit (Novagen, USA) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... L-histidine (L-his, Sigma-Aldrich #H8125), L-ornithine (L-orn ...
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bioRxiv - Molecular Biology 2024Quote: ... or pET30c (HIS) (Merck/Novagen, Darmstadt, Germany) using EcoRI and NotI sites ...
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bioRxiv - Microbiology 2021Quote: ... His-tagged recombinant proteins were detected using an HRP-conjugated anti-His monoclonal antibody (Sigma-Aldrich, St. Louis, MO) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... His- and GST-tagged proteins were detected with anti-His (Monoclonal Anti-polyHistidine antibody, Sigma H1029, 1:5000 dilution) or anti-GST (Mouse anti-GST monoclonal antibody ...
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bioRxiv - Immunology 2019Quote: ... and 5μl of either saline or 1μg/ml LPS in saline (0.005mg; from E.coli O111:B4, Sigma Aldrich) injected automatically into the striatum using a Hamilton syringe (Figure 1A) ...
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bioRxiv - Microbiology 2020Quote: ... and E.Coli HflX constructs were transformed into BL21(DE3)-T1R competent cells (Sigma-Aldrich, CAT No.:B2935) for protein expression ...
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bioRxiv - Cell Biology 2020Quote: ... Purification of both protein constructs was similar: plasmid was transformed into the Rosetta2 strain of E.coli (Novagen). Two liters of culture were grown in terrific broth for 8-12h at 37°C (until the OD600 ~2) ...
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bioRxiv - Neuroscience 2022Quote: Male and female mice received an intraperitoneal (i.p) injection of LPS (LPS from E.coli, O127:B8, Sigma) at 0.83 mg/kg ...
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bioRxiv - Biophysics 2019Quote: ... as a Tobacco Etch Virus (TEV)-cleavable N-terminal His-tagged (6×-His) fusion protein using a pET46 Ek/LIC vector (Novagen) and purified using Ni-NTA affinity chromatography and gel filtration chromatography as previously described (Hughes et al ...
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bioRxiv - Evolutionary Biology 2021Quote: ... A first purification step was performed using immobilized metal affinity chromatography with a His-bind resin column (His-Bond kit, Novagen) to recover PGM variants ...
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bioRxiv - Biochemistry 2023Quote: ... the protein was eluted in a gradient of His-AC washing buffer and His-AC elution buffer (both supplemented with 0.1% Tween-20 (Sigma Aldrich), 0.1% NP-40 (Thermo Fischer Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... His–NFR1CD–Myc or His–NFR1CD–K351E–Myc and NopT–FLAG–His were co–expressed by using Duet expressing vectors (Novagen) with 0.5 mM IPTG at 28°C for 18 h ...
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bioRxiv - Microbiology 2020Quote: ... as a His-tag fusion in pET28b (Novagen). Cells were grown in Terrific-Broth at 37°C to an OD600 = 1.2 and after 15 min at 4°C induced with 1 mM isopropyl β-d-thiogalactoside ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were detected using anti-His-HRP (Sigma) and anti-FLAG-HRP (Sigma ...
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bioRxiv - Plant Biology 2021Quote: ... -His with or without 3-AT (Sigma-Aldrich).
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bioRxiv - Pathology 2021Quote: ... cloned into a pET28a His-tag vector (Novagen) and expressed in E ...
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bioRxiv - Microbiology 2020Quote: ... Mouse anti-His antibody (1:12000) (Sigma-Aldrich) and biotinylated PNA (1:6000 ...
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bioRxiv - Biophysics 2020Quote: ... His-purifying resin was from Novagen (Darmstadt, Germany). Bis-(sulfosuccinimidyl ...
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bioRxiv - Cell Biology 2021Quote: ... and Anti-His(1:3000) antibody(Sigma #H1029).HRP conjugated mouse IgG was used at 1:4000 dilution(Sigma #A3673 ...
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bioRxiv - Neuroscience 2019Quote: ... and His-tagged human HDAC6 protein (EMD Millipore) were incubated with 57 nm 32P-ATP in kinase buffer (50 mM Tris HCl ...
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bioRxiv - Biochemistry 2021Quote: ... anti-His (Sigma H1029; 1:3000 for IB), anti-Hsp70 (Abcam ab47455 ...
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bioRxiv - Plant Biology 2022Quote: ... followed by blotting using Anti-His (SAB4301134, Sigma) and Anti-GST antibody (ab9085 ...
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bioRxiv - Genomics 2023Quote: ... with 10% heat-inactivated (HI) FBS (Sigma F0926). Vero/hSLAM cells were a kind gift from Dr ...
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bioRxiv - Systems Biology 2023Quote: ... MCF7 in DMEM-Hi Glucose medium (Sigma-Aldrich) HCC1937 and ZR-75-1 in RPMI 1640 media (Gibco) ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 2% GlutaMAX (200 mM ...
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bioRxiv - Cell Biology 2024Quote: ... +10% HI FBS(Sigma Aldrich, cat#F4135-500ML) + penicillin/streptomycin (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... An anti-His-primary antibody (Sigma, 1:2,000) was incubated with the protein-treated lipid strips to detect Jps1 bound via its C-terminal His-tag ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: All strains were maintained on OP50 E.coli seeded NGM agar supplemented with 50 IU/mL penicillin (Sigma #P4333), 50 µg/ml streptomycin (Sigma #P4333) ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were then stimulated with a cocktail of 500μg/mL lipopolysaccharide (LPS, isolated from E.coli O55:B5, Sigma) and 10μM inhibitors (MG132 ...
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bioRxiv - Immunology 2022Quote: ... Hybridization mix contained hybridization buffer (10% Dextran sulfate Sigma D8906, 30% Formamide, 1□mg/ml E.coli tRNA Sigma R1753 ...
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bioRxiv - Immunology 2020Quote: ... intravitreal injection with a 34-gauge Hamilton microsyringe through the pars plana was used to deliver 0.5 ng of lipopolysaccharide (LPS) from E.coli 055:B5 (Sigma) in a one microlitre volume of phosphate buffered saline (PBS ...
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bioRxiv - Immunology 2020Quote: Sorted neutrophils viability was evaluated after 1 h stimulation with 100ng/mL of LPS (E.coli 0111:B4, Sigma). Neutrophils were then incubated 15min at room temperature with anti-annexinV antibodies in binding buffer (BD Bioscience) ...
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bioRxiv - Genomics 2021Quote: ... Treated sections were then mounted with hybridization buffer (5%/15% Formamide; 10% Dextran sulfate Sigma D8906; 0.02% BSA Ambion AM2616; 1 mg/ml E.coli tRNA Sigma R1753 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated in hybridization buffer (10% Dextran sulfate Sigma D8906, 20% Formamide, 1mg/ml E.coli tRNA Sigma R1753 ...
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bioRxiv - Microbiology 2021Quote: ... and extensively dialysed against HEPES-buffered saline and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag mAb, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (Bethyl).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... thereafter referred to as Mpp75Aa1.1-His) or without the C-terminal His tag (thereafter referred as Mpp75Aa1.1) was cloned into a pET24a vector (Novagen, Madison, WI) and expressed in Rosetta 2 (DE3 ...