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Citations for Millipore Sigma :
2251 - 2300 of 10000+ citations for Human Dual Specificity Phosphatase 3 DUSP3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Werner syndrome helicase is a selective vulnerability of microsatellite instability high tumor cellsbioRxiv - Cancer Biology 2019Quote: ... and Phosphatase inhibitor cocktails (Sigma-Aldrich, St. Louis, MO, US; P5726 and P0044).
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bioRxiv - Biochemistry 2019Quote: ... and 1% NP-40 containing protein phosphatase inhibitor cocktail 1 and 2 (Sigma) and protease inhibitors (Sigma)] ...
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bioRxiv - Cell Biology 2019Quote: ... protease inhibitor mixture and phosphatase inhibitor mixture (Sigma-Aldrich, St Louis, MO, USA). Whole cell lysates obtained from the supernatant after microcentrifugation at 14,000 rpm for 15 min at 4°C were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Immunology 2020Quote: ... cells were lysed in lysis buffer with protease and phosphatase inhibitors (Sigma-Aldrich). Protein concentration in samples were quantified by BCA assay (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2021Quote: ... 0.1 % SDS) supplemented with protease and phosphatase inhibitors (catalog #P8340 and #P2850; Sigma), sonicated and cleared by centrifugation (10,000 × g ...
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bioRxiv - Neuroscience 2019Quote: ... 5 mM EDTA pH 8) supplemented with protease and phosphatase inhibitor cocktails (Sigma), using an ice-cold plastic cell scraper ...
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bioRxiv - Cancer Biology 2019Quote: ... and phosphatase inhibitor cocktail II and III (100x, Sigma-Aldrich, St Louis, MO) were added to the first tube ...
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bioRxiv - Plant Biology 2019Quote: ... α-RFP-biotin was detected using α-rabbit IgG–Alkaline Phosphatase (Sigma, a3687).
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bioRxiv - Immunology 2020Quote: ... Plates were developed by adding 50 µL of alkaline phosphatase substrate (Sigma Aldrich) for 15-30 min after 6 washes with PBS-T ...
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bioRxiv - Cell Biology 2019Quote: ... membranes were incubated with the respective alkaline phosphatase-conjugated secondary antibody (Sigma-Aldrich) for 1 h at RT and developed in a solution of nitroblue tetrazolium chloride (NBT ...
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bioRxiv - Cell Biology 2019Quote: ... supplemented with Roche protease/phosphatase inhibitor cocktail (Sigma-Aldrich, St. Louis, MO, USA)) for 30min on ice ...
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bioRxiv - Immunology 2019Quote: ... 2mM phenylmethylsulfonyl fluoride (PMSF) supplemented with phosphatase (Sigma-Aldrich, Saint Louis, MO, USA) and protease (Roche ...
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bioRxiv - Genetics 2019Quote: ... Secondary antibodies were alkaline phosphatase conjugates of goat anti-mouse IgG (Sigma, A3562); rabbit anti-goat IgG (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... 1% Triton X-100) supplemented with protease and phosphatase inhibitor cocktails (Sigma-Aldrich, Cat ...
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bioRxiv - Microbiology 2020Quote: ... A 1:2500 dilution of alkaline phosphatase conjugated α-rabbit secondary antibody (Sigma) in TBS-T 0.1% supplemented with 0.1% BSA was applied and blots incubated for 2 hours at room temperature followed by the washing procedure described above with the addition of a final 10 min ...
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bioRxiv - Biochemistry 2022Quote: ... 1% IGEPAL CA-630) supplemented with protease and phosphatase inhibitor cocktails (Sigma-Aldrich). 15-30 µg of total protein was loaded per well of precast NuPAGE gels (Life Technologies).
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bioRxiv - Biochemistry 2022Quote: ... The immobilized phosphatase was removed using a 0.22-micron spin filter (EMD Millipore). The protein was concentrated to less than 1 mL and was injected onto a Superdex 75 10/300 GL size exclusion column (GE Healthcare ...
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bioRxiv - Genetics 2022Quote: ... fortified with protease and phosphatase inhibitors (Sigma-Aldrich #4693132001 and Millipore Sigma #524627). Lysates were then centrifuged at 40C for 10 min at 13,000 rpm ...
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bioRxiv - Microbiology 2022Quote: ... Alkaline phosphatase conjugated anti-Rabbit IgG (1:5000) (Sigma-Aldrich, St. Louis, MO) was used as a secondary antibody ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 0.1% SDS) supplemented with protease inhibitors and phosphatase inhibitors (Roche, 04693132001; SIGMA, P5726) were added per 100mg of tissue and homogenized using a Polytron PT2100 benchtop homogenizer rotor ...
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bioRxiv - Neuroscience 2023Quote: ... The plasmid backbone was additionally treated with FastAP Thermosensitive Alkaline Phosphatase (Sigma Aldrich) after digestion to avoid re-ligation.
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bioRxiv - Biophysics 2024Quote: ... supplemented with protease and phosphatase inhibitor cocktail (Sigma, St. Louis, MO, USA, #P1860). Total protein concentration was determined with the BCA assay (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... I3021]) with addition of Complete protease and PhosSTOP phosphatase inhibitor (Sigma, 11697498001, 4906837001). A bicinchoninic acid assay (Fisher Scientific ...
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bioRxiv - Pathology 2024Quote: ... and phosphatase inhibitor cocktail (1:100; P5726, Sigma-Aldrich, St Louis, MO, USA) and homogenized on ice using a tight-fitting Teflon pestle ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with protease inhibitor and phosphatase inhibitor cocktail (Merck Millipore, Burlington, MA, USA) on ice ...
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bioRxiv - Developmental Biology 2024Quote: ... and 50 mM Tris pH 8.0) with protease and phosphatase inhibitor cocktails (Sigma, cat#11836170001 ...
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bioRxiv - Immunology 2024Quote: ... cells were lysed in lysis buffer with protease and phosphatase inhibitors (Sigma-Aldrich). Protein concentration in samples was quantified by BCA assay (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... each step supplemented with phosphatase inhibitor cocktail 2 (1:100, Sigma, cat#P5726). Samples were mounted in DABCO anti-fade medium prior imaging.
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bioRxiv - Cell Biology 2023Quote: ... The immunoprecipitated TFEB proteins were dephosphorylated by λ phosphatase (Cat# P9614, Sigma-Aldrich) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Secondary antibodies were Alkaline Phosphatase (AP)-conjugated anti-mouse (1:30,000, A3562, Sigma), and anti-rabbit antibodies (1:30,000 ...
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bioRxiv - Microbiology 2023Quote: ... Alkaline phosphatase conjugated anti-Rabbit IgG (1:5000) (Sigma-Aldrich, St. Louis, MO) was used as a secondary antibody ...
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bioRxiv - Molecular Biology 2023Quote: ... Digests were then treated with 10 units alkaline phosphatase (CIP, P4978, Sigma Aldrich), incubated for 1 hour at 37°C ...
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bioRxiv - Immunology 2023Quote: ... Alkaline Phosphatase (AP) activity was assayed using SIGMAFASTTM nitrophenyl phosphate tablets (Sigma-Aldrich) and the reaction was blocked with the addition of NaOH ...
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bioRxiv - Microbiology 2023Quote: ... followed by incubation with anti-rabbit secondary antibodies conjugated to alkaline phosphatase (Sigma). Reactions were visualized by the addition of Sigma fast 5-bromo-4-chloro-3-indolylphosphate-nitroblue tetrazolium.
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bioRxiv - Biochemistry 2022Quote: ... Alkaline phosphatase conjugated anti-Rabbit IgG (1:5000) (Sigma-Aldrich, St. Louis, MO) was used as a secondary antibody ...
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bioRxiv - Immunology 2023Quote: ... and secondary incubation with Goat anti-rabbit IgG Alkaline Phosphatase Antibody (Sigma, A3687).
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bioRxiv - Cancer Biology 2022Quote: ... and 1.5 mmol/L MgCl2) supplemented with protease and phosphatase inhibitors (EMD Millipore). The lysates were clarified by centrifugation ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 0.1 % (v/v) protease and phosphatase inhibitor cocktail (Sigma- Aldrich, US). Protein concentration was measured using the Bio-Rad protein assay.
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bioRxiv - Physiology 2023Quote: ... protease inhibitor cocktails and Ser/Thr and Tyr phosphatase inhibitor cocktails (Sigma, UK)] ...
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bioRxiv - Cell Biology 2023Quote: ... secondary antibodies were applied (goat anti-rabbit IgG-Alkaline Phosphatase, A9919, Sigma Aldrich; goat anti-mouse IgG-Alkaline Phosphatase ...
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bioRxiv - Neuroscience 2023Quote: ... followed by treatment of 1 unit of alkaline phosphatase (Cat# P5931, Sigma-Aldrich) for 2 h at 37 °C ...
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bioRxiv - Pathology 2023Quote: ... and endogenous alkaline phosphatase was quenched with 5 mM levamisole (L9756, Sigma-Aldrich). Preparations were then digested with 0.5% pepsin A (P7000 ...
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bioRxiv - Neuroscience 2024Quote: ... 1% Sodium Deoxycholate) with four protease or phosphatase inhibitors at 1% (Sigma-Aldrich, #P8340-5ML ...
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bioRxiv - Molecular Biology 2023Quote: ... containing protease and phosphatase inhibitor cocktails (cOmplete™, Mini Protease Inhibitor Cocktail, Sigma #11836153001 ...
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bioRxiv - Biochemistry 2023Quote: ... the membrane was incubated with an alkali phosphatase-bound chlorine-antibody (Sigma A3562), washed three times ...
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bioRxiv - Molecular Biology 2023Quote: ... EDTA-free protease and phosphatase inhibitor) together with 250 U Benzonase (Sigma Aldrich) per ml ...
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bioRxiv - Immunology 2023Quote: ... and 1% Triton X-100) supplemented with protease-phosphatase inhibitor cocktail (Sigma-Aldrich) and clarified by centrifugation at 7500 xg for 10 mins at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... containing a protease and phosphatase inhibitor cocktail (Sigma-Aldrich Inc., St. Louis, MO). After protein quantification (Pierce BCA Protein Assay Kit ...
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bioRxiv - Cancer Biology 2023Quote: ... containing a protease and phosphatase inhibitor cocktail (Sigma-Aldrich Inc., St. Louis, MO). Protein concentration was measured using the bicinchoninic acid (BCA ...
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bioRxiv - Immunology 2024Quote: ... Macrophages were lysed in RIPA buffer supplemented with protease and phosphatase inhibitor (Sigma) on ice for 10 minutes ...