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Citations for Millipore Sigma :
2151 - 2200 of 10000+ citations for Human Dual Specificity Phosphatase 3 DUSP3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Alkaline phosphatase conjugated anti-Rabbit IgG (1:5000) (Sigma-Aldrich, St. Louis, MO) was used as a secondary antibody ...
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bioRxiv - Microbiology 2023Quote: ... followed by incubation with anti-rabbit secondary antibodies conjugated to alkaline phosphatase (Sigma). Reactions were visualized by the addition of Sigma fast 5-bromo-4-chloro-3-indolylphosphate-nitroblue tetrazolium.
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bioRxiv - Immunology 2023Quote: ... Alkaline Phosphatase (AP) activity was assayed using SIGMAFASTTM nitrophenyl phosphate tablets (Sigma-Aldrich) and the reaction was blocked with the addition of NaOH ...
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bioRxiv - Biochemistry 2022Quote: ... Alkaline phosphatase conjugated anti-Rabbit IgG (1:5000) (Sigma-Aldrich, St. Louis, MO) was used as a secondary antibody ...
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bioRxiv - Immunology 2023Quote: ... and secondary incubation with Goat anti-rabbit IgG Alkaline Phosphatase Antibody (Sigma, A3687).
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bioRxiv - Cancer Biology 2022Quote: ... and 1.5 mmol/L MgCl2) supplemented with protease and phosphatase inhibitors (EMD Millipore). The lysates were clarified by centrifugation ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 0.1 % (v/v) protease and phosphatase inhibitor cocktail (Sigma- Aldrich, US). Protein concentration was measured using the Bio-Rad protein assay.
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bioRxiv - Physiology 2023Quote: ... protease inhibitor cocktails and Ser/Thr and Tyr phosphatase inhibitor cocktails (Sigma, UK)] ...
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bioRxiv - Cell Biology 2023Quote: ... secondary antibodies were applied (goat anti-rabbit IgG-Alkaline Phosphatase, A9919, Sigma Aldrich; goat anti-mouse IgG-Alkaline Phosphatase ...
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bioRxiv - Biochemistry 2023Quote: ... the membrane was incubated with an alkali phosphatase-bound chlorine-antibody (Sigma A3562), washed three times ...
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bioRxiv - Molecular Biology 2023Quote: ... EDTA-free protease and phosphatase inhibitor) together with 250 U Benzonase (Sigma Aldrich) per ml ...
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bioRxiv - Pathology 2023Quote: ... and endogenous alkaline phosphatase was quenched with 5 mM levamisole (L9756, Sigma-Aldrich). Preparations were then digested with 0.5% pepsin A (P7000 ...
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bioRxiv - Neuroscience 2023Quote: ... followed by treatment of 1 unit of alkaline phosphatase (Cat# P5931, Sigma-Aldrich) for 2 h at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... containing protease and phosphatase inhibitor cocktails (cOmplete™, Mini Protease Inhibitor Cocktail, Sigma #11836153001 ...
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bioRxiv - Developmental Biology 2023Quote: ... each step supplemented with phosphatase inhibitor cocktail 2 (1:100, Sigma, cat#P5726). Samples were mounted in DABCO anti-fade medium prior imaging.
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bioRxiv - Cell Biology 2023Quote: ... The immunoprecipitated TFEB proteins were dephosphorylated by λ phosphatase (Cat# P9614, Sigma-Aldrich) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... containing a protease and phosphatase inhibitor cocktail (Sigma-Aldrich Inc., St. Louis, MO). After protein quantification (Pierce BCA Protein Assay Kit ...
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bioRxiv - Immunology 2023Quote: ... and 1% Triton X-100) supplemented with protease-phosphatase inhibitor cocktail (Sigma-Aldrich) and clarified by centrifugation at 7500 xg for 10 mins at 4°C ...
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bioRxiv - Immunology 2024Quote: ... cells were lysed in lysis buffer with protease and phosphatase inhibitors (Sigma-Aldrich). Protein concentration in samples was quantified by BCA assay (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... and 50 mM Tris pH 8.0) with protease and phosphatase inhibitor cocktails (Sigma, cat#11836170001 ...
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bioRxiv - Cancer Biology 2023Quote: ... containing a protease and phosphatase inhibitor cocktail (Sigma-Aldrich Inc., St. Louis, MO). Protein concentration was measured using the bicinchoninic acid (BCA ...
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bioRxiv - Pathology 2024Quote: ... and phosphatase inhibitor cocktail (1:100; P5726, Sigma-Aldrich, St Louis, MO, USA) and homogenized on ice using a tight-fitting Teflon pestle ...
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bioRxiv - Neuroscience 2024Quote: ... 1% Sodium Deoxycholate) with four protease or phosphatase inhibitors at 1% (Sigma-Aldrich, #P8340-5ML ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with protease inhibitor and phosphatase inhibitor cocktail (Merck Millipore, Burlington, MA, USA) on ice ...
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bioRxiv - Neuroscience 2024Quote: ... I3021]) with addition of Complete protease and PhosSTOP phosphatase inhibitor (Sigma, 11697498001, 4906837001). A bicinchoninic acid assay (Fisher Scientific ...
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bioRxiv - Biophysics 2024Quote: ... supplemented with protease and phosphatase inhibitor cocktail (Sigma, St. Louis, MO, USA, #P1860). Total protein concentration was determined with the BCA assay (Thermo Fisher ...
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bioRxiv - Neuroscience 2021Quote: ... antisense: 5’-ACCGAUUGAAGUUAAUGUC-3’), Myef2 (sense: 5’-CUUUGGUGGAGUUGGCCGA-3’, antisense: 5’-UCGGCCAACUCCACCAAAG-3’) or siRNA universal negative control (SIC001, Sigma-Aldrich) were purchased from Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... Then the supernatant was separated by size to a large (>3 kDa) and small (<3 kDa) fraction using Amicon ultrafiltration system with 3 kDa filter (Millipore, Ireland). The fraction then filtered again through a 0.22 µm (Millipore).
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bioRxiv - Biochemistry 2023Quote: ... N-(3-Oxoocatnoyl)-L-HSL (>97%) (3-Oxo-C8) and N-(3-Oxododecanoyl)-L-HSL (>98%) (3-oxo-C12) were used as received from Sigma-Aldrich. N-(3-Hydroxy-7-cis-tetradecanoyl)-L-HSL (>95% ...
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bioRxiv - Cell Biology 2020Quote: ... Terminal deoxy-nucleotidyl transferase dUTP nick end labeling (TUNEL) was performed on transduced human islets (Apoptag In situ Apoptosis Detection Kit; Millipore) as per the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Sandwich ELISA kits for quantitative detection of human Aβ 1-40 peptides in a flexible 96 well format were purchased from Millipore (cat ...
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bioRxiv - Cancer Biology 2019Quote: ... Murine mCherry-G166 GSCs glioma cells or human G166 GSCs labelled with a Red Fluorescent Cell kit PKH26 (Sigma-Aldrich) were injected into NOD SCID mice ...
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bioRxiv - Cell Biology 2020Quote: Bacterial expression vectors for human Arf1 (UniProtKB: P84077) and Arf6 (UniProtKB: P62330) were constructed using a pET-41 Ek/LIC vector kit (Novagen), as described for protein expression of human Rab-family small GTPases (Tamura and Mima ...
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bioRxiv - Immunology 2023Quote: ... EasySep™ Human NK Cell Isolation Kit) and were frozen down in CS10 CryoStor cell cryopreservation media media (Sigma-Aldrich).
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bioRxiv - Cell Biology 2023Quote: ... Cytokine analysis was performed by Cincinnati Children’s Hospital Research Flow Cytometry Core per kit instructions (Milliplex MAP human protein panel, Millipore-Sigma).
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bioRxiv - Neuroscience 2023Quote: ... Cytokine levels were determined using a Milliplex bead assay kit (Milliplex MAP human cytokine/chemokine magnetic bead panel, #MCYTOMAG-70K; Millipore) using the Luminex MAPIX system with data analysis using xPONENT 4.2 software (Luminex) ...
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bioRxiv - Neuroscience 2023Quote: ... Aβ and pTau levels in the organoids were quantified with Milliplex human amyloid beta and tau kit (Millipore HNABTMAG-68K) on the Luminex ...
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bioRxiv - Biochemistry 2022Quote: ... The dual-affinity purified protein was concentrated via ultrafiltration using an Amicon Ultra-4 30 K device (Millipore), supplemented with BSA and glycerol to obtain final concentrations of 100 µg/mL and 50% (v/v) ...
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bioRxiv - Neuroscience 2023Quote: ... Beta-Mercaptoethanol Thermo Fisher Scientific 21985023) with double the concentration of dual-SMAD inhibitors (8uM Dorsomorphin Sigma-Aldrich P5499 ...
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bioRxiv - Immunology 2022Quote: A 96 well ELISA plate was coated with bovine fetuin (5 μg/mL; Sigma) in carbonate buffer overnight ...
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bioRxiv - Microbiology 2019Quote: ... supernatants were collected and analyzed for total GLP-1 levels by ELISA (Millipore Sigma) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... followed by the addition of the TMB/E ELISA substrate (Merck Millipore, Billerica, MA). Absorbance was measured as described.
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bioRxiv - Neuroscience 2023Quote: ... IAPP was measured by an enzyme-linked immunosorbent assay (ELISA; Merck Millipore, EZHA-52K).
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bioRxiv - Cell Biology 2020Quote: ... Lipin-1 siRNA 5’-GAAUGGAAUGCCAGCUGAA-3’ and 3’-UUCAGCUGGCAUUCCAUUC-5’ (Invitrogen; HSS118307 (Sigma); optineurin siRNA (Invitrogen 4392420) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Each mouse was intraperitoneal injected with 3 mL of 3% thioglycolate (Sigma-Aldrich) on day 1 and sacrificed using isoflurane on Day 3 ...
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bioRxiv - Neuroscience 2019Quote: ... Three additional small molecules were introduced on day 3: CHIR99021 (Sigma, 3 µM), SU5402 (R&D Systems ...
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bioRxiv - Neuroscience 2020Quote: ... after approximately 3 days in culture (DIV 3) cytosine β-D-arabinofuranoside (Sigma) was added to the hippocampal cultures in a 50% growth media exchange at a final concentration of 2 µM ...
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bioRxiv - Biochemistry 2021Quote: ... 1.7 g (8.9 mmol) 1-ethyl-3-(3- dimethylaminopropyl)carbodiimide (EDAC, Sigma Aldrich), and 1.2 g (8.9 mmol ...
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bioRxiv - Microbiology 2019Quote: ... and primers p181 (5’-GCGAAGATAACAGTGACTCTA-3’ and p54 (5’-CGGCTCTGATTAAATTCTGAAG-3’) (Sigma, UK).
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bioRxiv - Cell Biology 2021Quote: ... and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, 0.1 mg/ml, Sigma-Aldrich). Gels were rinsed 3 times with milli-Q water and dried in the oven for 15 min at 60 °C ...