Labshake search
Citations for Millipore Sigma :
2251 - 2300 of 3147 citations for 6 ISOPROPYLINDOLE 97 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2021Quote: ... The secondary antibodies were washed away with PBS (3x 5 min) whereafter 4’,6-diamidino-2-phenylindole (DAPI, 1:500, Sigma) was added for 10 minutes to localize the cell nuclei ...
-
bioRxiv - Biophysics 2020Quote: Purified K2P2.1cryst was concentrated to 6 mg ml-1 by centrifugation (Amicon Ultra-15, 50 kDa molecular mass cut-off; Millipore) and crystallized by hanging-drop vapor diffusion at 4 °C using a mixture of 0.2 μl of protein and 0.1 μl of precipitant over 100 μl of reservoir containing 20–25% PEG400 ...
-
bioRxiv - Molecular Biology 2019Quote: ... the cells were washed 2× in PBS and then counterstained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; 1:10 000; Sigma) for 10 min at room temperature ...
-
bioRxiv - Cell Biology 2020Quote: ... the medium was changed to S1 medium consisting of basal medium supplemented with 6 μM CHIR99021 (Sigma, Cat No. SML1046). Basal medium was prepared by adding 1x GlutaMax supplement (ThermoFisher ...
-
bioRxiv - Cell Biology 2020Quote: The following monoclonal antibodies were used for immunofluorescence: anti-acetylated tubulin (mouse; clone 6-11B-1; Sigma-Aldrich; 1:500), anti-ubiquitin clone FK2 was used in all experiments except where indicated (mouse ...
-
bioRxiv - Neuroscience 2021Quote: ... saline or 6-OHDA (5 μg/μL; 2 μL in total, at a rate of 0.5 μL/min, Sigma, Canada) were injected unilaterally in the dorsal striatum ...
-
bioRxiv - Cell Biology 2020Quote: ... the cells were incubated with 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI, cat. D8517, Sigma Aldrich, St. Louis, MO, USA) diluted 1:3000 in PBS 1X and then mounted with Vectashield antifade medium (cat ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were fixed using 70% Ethanol and re-suspended in PBS containing 1 μg/ml 4’,6-diamino-2-phenylindole (DAPI, Sigma) and analyzed by microscopy ...
-
bioRxiv - Microbiology 2020Quote: ... cells were analyzed using a Guava EasyCyte 6/2L bench cytometer equipped with 488 nm and 532 nm lasers (Millipore). Flow cytometry plots are representative of at least three independent experiments.
-
bioRxiv - Microbiology 2020Quote: ... Infected cultures were then either trypsinized for detection of GFP-positive cells and/or dextran-positive cells by flow cytometry on a Guava EasyCyte 6/2L bench cytometer (Millipore), or fixed with 4% paraformaldehyde and analyzed by fluorescence microscopy after labeling with antibodies specific for UIS4 (Sicgen ...
-
bioRxiv - Developmental Biology 2021Quote: ... embryos were rinsed several times in PBSDT and endogenous peroxydase was inactivated by a treatment with H2O2 6% (Sigma, H1009) during 30 min ...
-
bioRxiv - Neuroscience 2022Quote: ... and the supernatant was gently layered on a discontinuous Percoll gradient (2 %, 6 %, 10 %, and 20 % v/v in Tris-buffered 0.32 M sucrose; Sigma-Aldrich). After centrifugation at 33’500 g for 5 min ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... A volume of 40 μL (corresponding to 4.106 cells/cm2) of suspension was plated on 6 cm plates filled with 2 mL of 2% Phytagel (Sigma-Aldrich) as previously described by Dubravcic et al ...
-
bioRxiv - Cancer Biology 2022Quote: Lentivirus was titrated into 2 × 105 PC-3M cells on 6-well plates with 2 mL complete RPMI-1640 supplemented with 8 μg/mL polybrene (Sigma). After overnight infection ...
-
bioRxiv - Neuroscience 2020Quote: ... were prepared and then immediately enzymatically treated at 34°C with an initial incubation in a papain (6 mg/mL, Sigma) and dithioerythritol (10 mg/mL ...
-
bioRxiv - Neuroscience 2021Quote: ... For some experiments the AMPA/kainate glutamate receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 25 μM, Sigma-Aldrich), the NMDA glutamate receptor antagonist [3H]3-(2-carboxypiperazin-4-yl ...
-
bioRxiv - Neuroscience 2021Quote: ... nuclear labelling was achieved by incubating the sections for 5 min in DAPI (4′,6-diamidino-2-phenylindole; 1:5000; Sigma). Two final washes in PBS were performed before the sections were mounted on glass slides using Vectashield (Vector Laboratories ...
-
bioRxiv - Molecular Biology 2020Quote: ... Transfected cells were grown for 48 h in the presence of 7 µM ouabain (Sigma, O3125; CAS:11018-89-6), then diluted into 14 cm-dishes and grown for 2-3 weeks in the presence of ouabain to obtain isolated clones ...
-
bioRxiv - Molecular Biology 2020Quote: ... the MILLIPLEX® MAP 6-plex Bcl-2 Family Apoptosis Panel 1 Magnetic Bead Kit (Millipore: Cat. No. 48-682MAG) was utilized ...
-
bioRxiv - Molecular Biology 2021Quote: ... CLIP-UPF1 stable cell lines or a GFP-expressing control line were seeded in 6 × 15 cm plates and then treated with 200 ng/mL doxycycline hyclate (Sigma) for 48 hours to induce CLIP-UPF1 expression ...
-
bioRxiv - Developmental Biology 2022Quote: ... in PBST and then counterstained with 4’,6-diamidino-2-phenylindole (DAPI; 0.001 mg/mL in 1x PBS, Sigma-Aldrich) for 5 min in the dark ...
-
bioRxiv - Genomics 2020Quote: ... 4’,6-diamidino-2-phenylindole (DAPI) (5 μM) and propidium iodide (PI) (50 μg/mL) (Sigma-Aldrich, St. Louis, USA) and incubated at room temperature for 30-60 minutes ...
-
bioRxiv - Genomics 2020Quote: Electroporations were performed in 2-4 biological replicates into p2L RAR-DamA126 cells by mixing the listed DNA amounts with 5-20*10^6 cells in 300 ul EmbryoMax Electroporation Buffer (Millipore) and electroporating in a 0.4-cm electroporation cuvette using a BioRad electroporator at 230 V ...
-
bioRxiv - Immunology 2021Quote: ... cells were seeded 24 hours prior to transduction at a density of 50,000 cells per well in 6-well plates and transduced with lentiviral vectors in the presence of 5 µg/ml polybrene (Millipore). When cells were transduced with a Pro-Code library ...
-
bioRxiv - Immunology 2021Quote: Blood was extracted from hBLT mice at 3 and 5-6 weeks post-infection and lysed with Red Blood Cell Lysis Buffer (SIGMA). T cells were activated for 1.5 h with 5μl/ml of anti-CD28 and anti-CD49d in the presence or absence of 6.4μg/ml of a Gag pool of peptides in the presence of 0.5μg/ml Brefeldin A ...
-
bioRxiv - Immunology 2021Quote: ... 1-2 x 106 mononuclear cells isolated from blood or tonsil samples were cultured in a round bottom 96 well plate at 37°C 5% CO2 in R10 media for 6 hours with or without 200 ng/ml phorbol myristate acetate (PMA) and 1.0 μg/ml Ionomycin (Sigma-Aldrich) or 0.5 μM/ml 5-amino-6-D-ribitylaminouracil (5-A-RU ...
-
bioRxiv - Molecular Biology 2021Quote: ... 4×106 ESCs were allowed to attach to gelatinised 15cm dishes for 6-8 hours and treated with 1μM all-trans retinoic acid (Sigma-Aldrich) in EC-10 medium (DMEM supplemented with 10% fetal bovine serum ...
-
bioRxiv - Molecular Biology 2019Quote: Embryos were incubated in 1:5000 acridine orange solution for 1 hr at 28.5°C (stock: 6 mg/ml, Sigma-Aldrich) followed by 2X washes in fish system water ...
-
bioRxiv - Immunology 2019Quote: ... adult animals (6-12 weeks) were perfused with 25 mL of PBS (Hyclone) and 25 mL of 4% paraformaldehyde (PFA, Sigma) sequentially at room temperature (RT) ...
-
bioRxiv - Neuroscience 2019Quote: ... mononuclear cells (1×106 cells/mL) were cultured for 6 h with or without phorbol 12-myristate 13-acetate (PMA, 50ng/mL; Sigma), ionomycin (50ng/mL ...
-
bioRxiv - Biophysics 2019Quote: ... The reaction was started by adding 20 µl of 500 µM DHF (Sigma Aldrich, cat# D7006, CAS: 4033-27-6) in MTEN with 5 mM DTT to 180 µL of pre-reaction mixture ...
-
bioRxiv - Cell Biology 2019Quote: ... Covalently modified αSYN was separated from non-reacted dye on a Sephadex G-25 column (Amersham, GE) and concentrated with 6 kDa MW cut-off spin columns (Millipore). The final concentration of the fluorescently labeled protein was measured by UV-VIS spectrophotometry at 280 nm with E ...
-
bioRxiv - Bioengineering 2019Quote: Cy3-(GT)6-SWCNTs and Cy3-(GT)30-SWCNTs were first filtered 3 times using 100kDa Amicon centrifuge filters (Millipore) to remove free Cy3-DNA from solution ...
-
bioRxiv - Bioengineering 2019Quote: ... Laccase activity was followed by the oxidation of 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) (Sigma-Aldrich, USA) (33) ...
-
bioRxiv - Cancer Biology 2020Quote: Cre expression and activity was induced in 6-8 week old Krt19-CreERT and Rosa26-CreERT mice by intraperitoneal injection of tamoxifen (Sigma) dissolved in corn oil (Sigma ...
-
bioRxiv - Microbiology 2019Quote: ... Viral size particles in the filtrate were concentrated to ~500 mL (viral concentrate) using tangential flow ultrafiltration with a 30kDa MW prep-scale Spiral Wound TFF-6 cartridge (Millipore) (53).
-
bioRxiv - Cell Biology 2019Quote: Cold APC purification buffer (100 mM NaPi, Sigma#S3139 and Sigma#S9390, 300 mM KCl, Sigma#P9333, 5 mM MgCl2 × 6 H2O, Sigma#M2670 ...
-
bioRxiv - Developmental Biology 2019Quote: ATDC5 were plated in 6-well plates to 80% confluency and lentiviral transduction was using diluted viral media with 0.1% polybrene (EMD Millipore) for 24 hours followed by selection with 4μg/ml Blastocidin and Puromycin for 5 days post transfection ...
-
bioRxiv - Biochemistry 2020Quote: Full length human KBP residues 1-621 in a PSTCm1 expression vector (with kanamycin resistance and a N-terminal thrombin cleavable 6 x His-tag) was expressed in Rosetta2 cells (Novagen). Following immobilised metal-affinity chromatography with Ni-NTA resin (Qiagen) ...
-
bioRxiv - Molecular Biology 2019Quote: ... cells were washed three times in PBS and mounted in 70% glycerol with with 5 μgml-1 concentration of 4-6-diamidino-2-phenylindole (DAPI, Sigma) and cells were mounted on cover slips ...
-
bioRxiv - Molecular Biology 2019Quote: Bloodstream parasites were spun down and resuspended in differentiation media (19) supplemented with 6 mM cis-aconitate (Sigma-Aldrich A3412) at 27°C for three days.
-
bioRxiv - Biochemistry 2019Quote: ... 3 mM KCl) and harvested in a PBS lysis buffer (PBS plus 6 uL/mL protease inhibitors cocktail (Sigma P8340), 0.6 mM PMSF ...
-
bioRxiv - Genomics 2019Quote: ... 1.5 g/L (days 0-5 and days 11-28) or 2.5 g/L (days 6-10) NaHCO3 (Sigma-Aldrich), and 0.25 mM (days 3-10 ...
-
bioRxiv - Neuroscience 2019Quote: 12 female young adult C57BL/6 mice (mean age 2.2 months) were sedated intraperitoneally with chlorprothixene (0.5 mg/kg, Sigma-Aldrich, UK) and anaesthetised with isoflurane (2% for induction ...
-
Molecular coevolution of nuclear and nucleolar localization signals inside basic domain of HIV-1 TatbioRxiv - Evolutionary Biology 2021Quote: ... pH 7.4) containing 10 mM sodium azide and 6 mM 2-deoxy-D-glucose (Sigma-Aldrich, St. Louis, MO, USA) and incubated for 50 min ...
-
bioRxiv - Cancer Biology 2019Quote: ... 6 week-old mice were injected intraperitoneally three times at one day interval with tamoxifen (T5648; Sigma-Aldrich, Bornem, Belgium) dissolved in corn oil at a concentration of 30 mg/mL and at a dose of 150 mg/kg of body weight ...
-
bioRxiv - Immunology 2021Quote: ... Monolayers were then rinsed thoroughly with Promocell DC generation medium to remove non-adherent cells and subsequently were cultured for 6 days in dendritic cell generation medium supplemented with cytokines (Sigma) to generate immature MoDCs.
-
bioRxiv - Bioengineering 2021Quote: ... cells were cultured for different periods (6, 12 or 24 hours) in different combinations of vascular endothelial growth factor (VEGF, human recombinant; Millipore) and brain-derived neurotrophic factor (BDNF ...
-
bioRxiv - Neuroscience 2020Quote: ... Spontaneous IPSCs were isolated by blocking AMPA receptor-mediated events with CNQX (6-cyano-7-nitroquinoxaline-2,3-dione; 20uM; Sigma-Aldrich). Miniature events were isolated by blocking sodium channels with the addition of tetrodotoxin (1uM ...
-
bioRxiv - Microbiology 2021Quote: Plaque size analysis were performed in HEK293T Spir-1 WT and KO cells seeded in 6-well plates coated with poly-D-lysine (Sigma). Once confluent ...