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Citations for Millipore Sigma :
2051 - 2100 of 3147 citations for 6 ISOPROPYLINDOLE 97 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Three-month-old C57BL/6 male mice were injected with 10 µg BrdU (Sigma-Aldrich, St. Louis, MO; www.sigmaaldrich.com)/gram body weight intraperitoneally every 12 hours for 2 days before sacrifice ...
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bioRxiv - Cell Biology 2020Quote: ... and (1uM) Clemastine Fumarate D3:D20 (Cayman) for 6 weeks (M2; the optimal media) and (2.5ug/ml) Laminin (Sigma), and (1 ug/ml ...
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bioRxiv - Cell Biology 2020Quote: ... Following staining with secondary antibodies and 0.1 µg/ml 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI, Sigma, D-9542) diluted in 5% NGS-PBS ...
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bioRxiv - Cell Biology 2020Quote: ... cells were transfected with a total of 1000 μg to 1500 μg DNA in 35 mm fluorodishes or 6 well plates using Genejuice as per the manufacturer’s instructions (Merck Millipore). Cells were typically imaged 48 h after transfection ...
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bioRxiv - Genetics 2021Quote: ... and embryos from natural spawning were injected with up to 600pg RNA in phenol red (P0290, Sigma, 1:6). Injected embryos were maintained at 28.5°C in egg water and collected at 26hr for histology ...
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bioRxiv - Neuroscience 2020Quote: ... Each IHC staining round was accompanied by nuclear cell counterstaining with 4’,6-diamidino-2-phenylindole (1μg/ml DAPI (Sigma) diluted 1:1000 in PBS) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 0.1% w/v Ferrozine (Disodium-4-[3-pyridin-2-yl-6-(4-sulfonatophenyl)-1,2,4-triazin-5-yl]benzosulfonate (Sigma-Aldrich) in dd ...
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bioRxiv - Microbiology 2022Quote: ... the larvae were kept in 6-well plates containing egg water (60 μg/mL sea salts (Sigma-Aldrich; S9883) in demi water ...
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bioRxiv - Cell Biology 2022Quote: ... rinsed in PBS and incubated with 3 μg/ml DAPI (4’,6-diamidino-2-phenylindole; D8417 from Sigma Aldrich) at RT for 30min ...
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bioRxiv - Cell Biology 2022Quote: ... Actin was visualized by FluoProbes 547H (557/572nm) coupled Phalloïdin (Interchim) and nuclei with 0.2 μg/mL 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich). Coverslips were mounted in Mowiol (Sigma-Aldrich).
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bioRxiv - Neuroscience 2021Quote: ... Cells were plated at a density of 6×104 cells / cm2 on glass coverslips coated with 0.2mg/ml Poly-L-lysine (#P2636-100MG Sigma) made in Borate buffer pH 8.5 ...
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bioRxiv - Neuroscience 2021Quote: ... The neurons were quantified and centrifuged at 1,800 rpm for 6 min and then plated on the glass coverslips pre-coated with poly-L-lysine (Sigma). The culture medium was Neurobasal (Gibco ...
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was washed in a pre-hybridization solution (6 x SSC, 1 x Denhardt’s, 100 μg/ml denatured Herring Sperm DNA (Sigma) and 0.5 % SDS ...
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bioRxiv - Molecular Biology 2020Quote: ... Reconstituted complexes were purified over a Superose 6 increase 3.2/300 size exclusion column SEC in GF150 buffer and immediately crosslinked in 0.025% Glutaraldehyde (Sigma-Aldrich) for 30 minutes on ice followed by quenching with 1 mM Tris-HCl (pH 7.4) ...
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bioRxiv - Developmental Biology 2022Quote: ... and 37°C with daily medium change and split twice a week in a 1:6 ratio using 0.05% Trypsin-EDTA (Sigma).
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bioRxiv - Cell Biology 2022Quote: ... the nucleus was stained by incubation with 4’,6-diamidino-2-phentylindole (DAPI, Sigma, 200 ng/ml in PBS) for 1 min at RT ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Embryos were washed in TBS stained with 4’,6-diamidino-2-phenylindole (DAPI) (1:1000, REF-D9542, Sigma, USA) for 30 min at RT ...
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bioRxiv - Physiology 2019Quote: ... The study included five treatment groups (n≥6 per group) receiving either 0.24 g/kg bodyweight bovine fetuin-A (Sigma) or 0.10 g/kg bodyweight PPi (Sigma ...
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bioRxiv - Neuroscience 2019Quote: ... and kinate receptors was achieved with 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo[f]quinoxaline-2,3-dione (NBQX; 50 µM; Sigma, Australia), N-methyl-D-aspartate (NMDA ...
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bioRxiv - Plant Biology 2019Quote: ... Fe(III)-cellulose (5 mM FeCl3 + 50 mM cellulose, pH 5.8, bought from Sigma, CAS number: 9004-34-6), Fe(III ...
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bioRxiv - Physiology 2019Quote: ... Eight-week-old male C57Bl/6 mice (Janvier Laboratories, France) were used for pharmacological experiments with spironolactone (Sigma-Aldrich), eplerenone (Inspra ...
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bioRxiv - Immunology 2019Quote: ... spleens from C57BL/6 mice were cut to smaller pieces and digested with Collagenase D (2 mg/ml, Sigma) For 30 min at 37°C ...
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bioRxiv - Developmental Biology 2020Quote: ... resuspended in complete Neurobasal media and plated at 0.5 × 106 cells/well on 6-well plates coated with 0.1 mg/ml poly-L-lysine (PLL; Sigma Aldrich). Neurons were cultured at 37°C in a humidified incubator with 5% CO2 and medium replaced every 5 days with fresh medium containing B27.
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bioRxiv - Cancer Biology 2019Quote: ... cells were seeded in 6-well plates and transfected the next day with 30pmol of ESR1 (Sigma-Aldrich, SASI_Hs01_00078592), ESR2 (Dharmacon ...
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bioRxiv - Immunology 2019Quote: ... diluted 1:150 in washing buffer (45 min) and 4’,6-diamidino-2-phenylindole counterstain (DAPI; Roche/Sigma-Aldrich) diluted 1:250 in TBS (15 min ...
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bioRxiv - Cell Biology 2019Quote: ... Following washing the tissues were counterstained with 1 μg/mL DAPI (4–6-diamidino-2-phenyl indole, Sigma-Aldrich) and mounted with PromoFluor Antifade Reagent (PromoKine).
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bioRxiv - Biochemistry 2019Quote: ... catalog number G1112 and an electron coupling reagent (PMS) [CAS: 299-11-6] from Sigma (St. Louis, MO,USA).
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bioRxiv - Biophysics 2019Quote: ... Dead cells were removed on day 6 after T cell isolation through density-dependent gradient centrifugation (Histopaque 1119, Sigma) and experiments were conducted between day 7 and day 9 after isolation.
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bioRxiv - Cancer Biology 2019Quote: ... Cross-linked pellets were thawed and lysed on ice for 30 min in ChIP harvest buffer (12 mM Tris-Cl, 1 × PBS, 6 mM EDTA, 0.5% SDS) with protease inhibitors (Sigma). Lysed cells were sonicated with a Bioruptor (Diagenode ...
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bioRxiv - Molecular Biology 2019Quote: ... male Wistar rats (n=5-6/group) were deeply anesthetized with sodium phenobarbital (Sigma-Aldrich; 50 mg/kg i.p.), and then positioned in a stereotaxic frame (TrentWells ...
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bioRxiv - Neuroscience 2021Quote: ... band power values in each frequency band (Delta:1-4Hz; Theta:6-9.5Hz; Sigma:10-16Hz; Gamma:30-60Hz) were divided by a normalization factor that corresponds to the sum of the power across all the frequencies according to the experimental condition ...
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bioRxiv - Developmental Biology 2020Quote: ... mice were fasted for 6 hours prior to IP injection of 2 g/kg 20% D-glucose (Sigma-Aldrich) in water with a 26G needle ...
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bioRxiv - Immunology 2021Quote: ... cells were stimulated with HDM or the known AhR agonist FICZ (5,11-Dihydroindolo[3,2-b]carbazole-6-carboxaldehyde, Tocris Bioscience, Bristol, UK or Sigma-Aldrich) to activate the AhR and induce CYP1A1 transcription ...
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bioRxiv - Immunology 2021Quote: ... Cell suspensions were incubated with 2 μg/mL 4’,6-diamidine-2’-phenylindole dihydrochloride (DAPI, Sigma-Aldrich, Missouri, USA) for 10 minutes as the final step in all flow cytometry assays to distinguish membrane integrity and disruption ...
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bioRxiv - Plant Biology 2020Quote: ... Electrodes with fine tips (Resistance = 4 to 6 GΩ) were filled with ion-selective ionophore cocktails (Sigma, Buchs, Switzerland) and their tips were moved towards and away from the sample in a slow (5 s cycle ...
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bioRxiv - Plant Biology 2020Quote: ... and infiltrated with a solution containing 240 mM sorbitol and 6 mg.L-1 Lucifer Yellow CH dipotassium salt (LYCH, Sigma), which is used as a tracer to normalize apoplastic fluids (Derrick et al. ...
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bioRxiv - Biochemistry 2021Quote: ... The culture supernatant containing the target protein was concentrated 3-fold and buffer exchanged against 6 volumes of working buffer (50 mM Tris/HCl, 300 mM NaCl, pH 8.0) by crossflow filtration (Millipore Pellicon 2 mini filter ...
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bioRxiv - Immunology 2020Quote: 1×107 draining lymph node or spleen cells were restimulated for 6 hours with 10μM MCC peptide with the addition of 5μM Brefeldin A (Sigma Aldrich) for the final 4 hours ...
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bioRxiv - Immunology 2021Quote: ... Nuclei were then counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich, catalog no. D9542, 100 ng/ml). The stained tissue sections were scanned using an Olympus VS-120 slide scanner and imaged using a Hamamatsu ORCA-R2 C10600 digital camera for all bright-field and fluorescent images ...
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bioRxiv - Neuroscience 2020Quote: ... The oxygen electrodes were calibrated in air-saturated 0.9% sodium chloride (at 37°C) and oxygen-free standard solution [0.1M sodium hydroxide (SX0607H-6, Sigma-Aldrich) and 0.1M sodium ascorbate (A7631 ...
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bioRxiv - Cancer Biology 2021Quote: ... and 1% Glycerol) containing a 3-fold dilutions (from 0.125 units/ml to 6 units/ml) of Benzonase nuclease (Millipore). This was mixed gently and incubated for three minutes at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 mM MgCl2 6 H2O and 5-Bromo-4-chloro-3-indolyl β-D-galactoside or X-gal (Sigma, B4252 ...
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bioRxiv - Biochemistry 2021Quote: ... EMSA reactions were then run on a non-denaturing 0.5X TBE 6% polyacrylamide gel and stained with ethidium bromide (Sigma). Band intensities were quantified using ImageJ.
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bioRxiv - Cell Biology 2021Quote: ... the cells were split into 6-well plates at a density of 0.5×106 cells/well and subjected to 2µg/mL puromycin (Sigma P8833) for selection of infected cells ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were extracted using 1.6 ml of extraction/washing buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.1% Triton, 0.2% NP-40, 6 mM BME and 1x protease inhibitor cocktail [SIGMA, USA]) during 30 minutes at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Lysates were resolved on 4-12% Bis-Tris Gels (Thermo) or homemade 6% Bis-Tris gels and transferred to 0.45 μm PVDF (Millipore) membranes ...
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bioRxiv - Cell Biology 2020Quote: ... and a thrombin solution containing CaCl2 and aprotinin (final concentration of fibrin components: 6 mg/mL fibrinogen (Sigma-Aldrich); 2 NIH U/mL thrombin from human plasma (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2020Quote: ... and pre-cultivated on MS1 medium[MS0 with 0.1 g/L IAA (Sangon Biotech Co., Ltd, Shanghai, China) and 1.5 g/L 6-BA (Sigma, USA)] ...
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bioRxiv - Microbiology 2020Quote: ... and naïve CD4+ T cells (6 × 104 cells/well) were plated together with 0.1 nM retinoic acid (Sigma-Aldrich) and 2 ng/ml recombinant mouse TGF-β (R&D Systems) ...
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bioRxiv - Microbiology 2020Quote: ... The epithelial cell proteins that remained bound to the fungal cells were eluted twice with 6 M urea (Sigma). The proteins were then separated on 10% SDS-PAGE and transferred to immun-Blot PVDF Membrane (BIO-RAD) ...