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Citations for Millipore Sigma :
1951 - 2000 of 10000+ citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Minced pancreas samples from NOD mice were digested in RPMI-10 supplemented with 5 mg/ml Collagenase Type V (Sigma) and 10 ug/ml DNase I followed by incubation in enzyme-free cell dissociation buffer (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... A catheter was inserted into the distal end of the aortotomy and used to infuse the aorta with sterile isotonic saline (NaCl, 0.9%) containing type I Porcine Pancreatic Elastase (2.5 U/mL) (Sigma-Aldrich, #E1250) at 120 mm Hg for 5 min ...
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bioRxiv - Cell Biology 2023Quote: Wild-type HeLa Kyoto cells (RRID: CVCL_1922) were incubated in Dulbecco’s Modified Eagle Media (DMEM with low glucose, Sigma, D6046-500ML) supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were resuspended and plated onto 10 µg/mL fibronectin- or rat tail collagen type I-coated glass bottom dishes (Sigma). Cells were fixed the next day with 4% paraformaldehyde ...
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bioRxiv - Neuroscience 2023Quote: ... wild-type C57BL/6N mice received intraperitoneal injections of a solution (10ml/kg) containing desipramine hydrochloride (2.5 mg/ml, Sigma-Aldrich) and pargyline hydrochloride (0.5 mg/ml ...
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bioRxiv - Bioengineering 2024Quote: ... Tumor nodules from each mouse were diced with scissors then incubated for one hour at 37 °C in 2 mL of 1 mg/mL collagenase type IV (Sigma) in RPMI media ...
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bioRxiv - Genetics 2024Quote: ... The liver tissue was then digested with a KRG buffer containing 2 mM CaCl2 and 0.5 mg/mL collagenase type IV (Sigma-Aldrich). Then ...
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bioRxiv - Biochemistry 2024Quote: ... washed one time with 40 ml cold 1 M sorbitol and resuspended in a Ficoll buffer (18% Ficoll type 400 (Sigma), 20 mM KH2PO4 pH 6.8 ...
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bioRxiv - Immunology 2024Quote: ... the peritoneal cavity was opened and the liver was perfused in situ via the portal vein at 37°C with 20 ml PBS followed by 20 ml DMEM containing 10 mg collagenase (type IV, Sigma). The liver was then removed and gently minced ...
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bioRxiv - Cell Biology 2024Quote: ... cells were dislodged with 1x trypsin (HiMedia) and cultured on polyacrylamide (PA) hydrogels coated with rat tail Collagen type I (Sigma) and/or low molecular weight (8-15 kDa ...
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bioRxiv - Bioengineering 2024Quote: ... the ideal support bath was prepared by mixing 2% w gelatin type B from bovine bone 250 LBB8 (Rousselot Biomedical) with 0.5% gum arabic (G9752, Sigma-Aldrich) in 50% v of the total solvent (deionized water ...
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bioRxiv - Cancer Biology 2024Quote: ... Fibroblasts were isolated from normal human skin and immortalized using human papilloma virus type oncogenes E6/E7 as previously described66 and cultured at 37 °C and 5% CO2 in DMEM (Sigma) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2024Quote: A549 wild-type cells (A549 WT, ATCC: CCL-185) and A549 cells endogenously expressing GFP-tagged EGFR (Sigma-Aldrich, CLL1141) were cultured and maintained in DMEM (Gibco ...
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bioRxiv - Immunology 2024Quote: ... lungs were minced and transferred to 50 mL tubes containing 2 mL of digestion buffer (1 mg/mL type IV collagenase and 0.1 mg/mL DNase [Sigma-Aldrich] in Dulbecco’s PBS [DPBS] with calcium and magnesium) ...
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bioRxiv - Cancer Biology 2024Quote: ... xenograft tumors excised from mice were weighed before immediate mechanical and enzymatic dissociation in DMEM-collagenase media for 1 hour in a 37°C shaker at 150 rpm (Hyclone DMEM, 2 mg/mL Collagenase type IV (Sigma), 5% FBS (Atlas Biologicals) ...
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bioRxiv - Neuroscience 2024Quote: ... All animals were injected in the L2-L3 spinous ligament region via Hamilton syringe with 5 microliters (µL) of either vehicle (0.9% saline) or urokinase-type plasminogen activator (uPA, 2mg/ml, CC4000, Millipore Sigma) under anesthesia (4% isoflurane and 2 liters of oxygen) ...
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bioRxiv - Biophysics 2024Quote: ... the apical surface of the preparation was exposed for 20 min to endolymph supplemented with 67 μg ⋅ mL−1of the protease subtilisin (type XXIV, Sigma). The otolithic membrane was then peeled off to obtain access to individual hair bundles ...
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bioRxiv - Immunology 2024Quote: ... the tumors were incubated at 37°C for 30 min in 10 mL of RPMI supplemented with 10% heat-inactivated FBS and 1% penicillin-streptomycin as well as 20 μg/mL DNase I type IV (Millipore) and 1 mg/mL collagenase from Clostridium histolyticum (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2024Quote: ... Fresh human subcutaneous lipoaspirate from patients undergoing elective liposuction surgery was processed by digestion in collagenase Type IA (Sigma Aldrich), and then density centrifugation was used to isolate the stromal vascular fraction ...
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bioRxiv - Bioengineering 2024Quote: ... sodium alginate (Protanal LF 10/60 FT, FMC BioPolymer) and 7% (w/v) gelatin from bovine skin Type B (Sigma) sterile powder were dispersed into Dulbecco’s phosphate buffer saline (DPBS ...
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bioRxiv - Genomics 2024Quote: ... The biopsy was minced into 1-2 mm3 pieces and digested with 0.7 mg/ml type 1A collagenase (Sigma C9891) in culture media at 37°C for 45 min with gentle agitation every 15 min ...
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bioRxiv - Developmental Biology 2024Quote: ... and kidneys were homogenized with scissors and digested in 10 mL RPMI including 1:10 Collagenase Type IV (60U f.c., Sigma-Aldrich), 25 µg/ml Dispase I (Roche) ...
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Dissecting the regulatory logic of specification and differentiation during vertebrate embryogenesisbioRxiv - Developmental Biology 2024Quote: ... ∼50 embryos per experimental condition were dechorionated using 1 mg/ml Pronase (Protease type XIV from Streptomyces griseus, Millipore Sigma). At 50% epiboly ...
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bioRxiv - Cell Biology 2024Quote: ... Muscles were digested mechanically using surgical scissors and incubated for 90 min at 37⁰C with agitation in collagenase type V (Sigma) and dispase II (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were separated by 8% to 12% SDS–PAGE and transferred to Immobilon-P PVDF membrane (Millipore). Blocking and probing with antibodies were performed as previously described (Iréna Lassot et al. ...
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bioRxiv - Cell Biology 2020Quote: ... mOSE cells were cultured in stem cell media [Dulbecco’s Modified Eagle’s Medium: Nutrient Mixture F-12 (Sigma) supplemented with 1 X B27 supplement (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... and maintained in imaging medium composed of F-12 Ham Nutrient Mixture with 25 mM HEPES (Sigma), the latter to compensate for the lack of CO2 ...
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bioRxiv - Developmental Biology 2021Quote: ... adult females at 5-12 month old were anesthetized in 550 µg/ml tricaine (Sigma, Cat A5040) in a petri dish ...
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bioRxiv - Molecular Biology 2022Quote: ... Thalassiosira pseudonana 1085/12 was grown in f/2 plus silica and 0.6 μM cyanocobalamin (Millipore-Sigma) at the same temperature and light regime ...
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bioRxiv - Neuroscience 2022Quote: ... cells were treated either with or without 600 nM phorbol-12-myristat-13-acetat (PMA, Sigma-Aldrich) for 3 h and OCR and ECAR were determined in parallel ...
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bioRxiv - Immunology 2022Quote: ... differentiated by treatment with 100 μM of phorbol 12-myristate 13-acetate (PMA, Sigma, St. Louis, MO) for 24 hrs ...
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bioRxiv - Physiology 2020Quote: ... The non-patterned hydrogel was made by dissolving 12%w/v porcine gelatine (Sigma, St. Louis, MO) in ddH2O by heating to 65°C and dissolving 10% w/v solution of microbial transglutaminase (MTG ...
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bioRxiv - Microbiology 2021Quote: ... The lipid films were rehydrated with 12 mL of 0.7 M clodronate (Sigma Aldrich, St Louis, MO) prepared in degassed sterile water for injection (Hospira ...
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bioRxiv - Microbiology 2021Quote: ... THP-1s were differentiated with 50 ng mL-1 phorbol 12-myristate 13-acetate (PMA, P1585, Sigma) for 3 days and then rested for 2 days in PMA-free ...
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bioRxiv - Biochemistry 2020Quote: ... 18 mm #1 coverslips (Fisher 12-545-100) were etched in 3M sodium hydroxide (Millepore Sigma 221465) prior to cell plating ...
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bioRxiv - Neuroscience 2020Quote: ... separated on 4-12% Bis-Tris Mini Gel (NuPAGE) and transferred to a PVDF membrane (Millipore #ISEQ00010). Nonspecific binding was blocked for 1 hr at RT with 5% BLOTTO (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: Mouse splenic B-cells were pre-treated or not with 12 μM bromoenol lactone (BEL, Sigma-Aldrich) in DMEM-BSA for 30 min at 37°C and then incubated with Tf-beads (1:2 cell-bead ratio ...
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bioRxiv - Microbiology 2020Quote: ... THP-1 cells were differentiated with 50 ng/mL phorbol 12-myristate 13-acetate (PMA, P1585, Sigma) for 3 days followed by a rested for 2 days in complete medium without PMA ...
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bioRxiv - Microbiology 2020Quote: ... THP-1 monocytes were differentiated into macrophages with 50ng/ml PMA (Phorbol 12-myristate 13-acetate) (Sigma) for 16 hours followed by 24 hours of incubation in complete medium (Mehto et al. ...
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bioRxiv - Cell Biology 2020Quote: T cells were stimulated with a combination of Phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich, P8139) and ionomycin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... Islets were isolated from 6-12 week old male and female FVB mice using collagenase XI (Sigma) digestion according to a previously published protocol101 ...
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bioRxiv - Pathology 2022Quote: ... Animals were administered an MMP-12 inhibitor (MMP-408; 10 mg/kg, i.g.; Merck Millipore, Burlington, MA) or vehicle (saline ...
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bioRxiv - Molecular Biology 2022Quote: ... Thalassiosira pseudonana 1085/12 was grown in f/2 plus silica and 0.6 μM cyanocobalamin (Millipore-Sigma) at the same temperature and light regime ...
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bioRxiv - Cancer Biology 2022Quote: T cells were stimulated with 5 ng/ml of phorbol 12-myristate 13-acetate (PMA, Sigma Aldrich) and 250 ng/ml of ionomycin (Sigma Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... and then seeded on a coverslip (12 mm diameter) in Dulbecco’s modified Eagle’s medium (DMEM; Sigma-Aldrich) supplemented with 10% (vol/vol ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were PCR-amplified with 10-12 cycles using KOD Hot Start DNA polymerase (Millipore, 71086-3) and cleaned up using 1X KAPA Pure Beads ...
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bioRxiv - Immunology 2020Quote: ... For panels evaluating response to activation 10 ng/ml phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich) and 1 µg/ml of ionomycin were used to stimulate cells for 4 hours at 37°C 5% CO2 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HepG2 Cells were grown and maintained in Dulbecco’s Modified Eagles’s Medium Nutrient Mixture F-12 Ham (Sigma) supplemented with L-glutamine and 15mMHEPES ...
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bioRxiv - Microbiology 2021Quote: 12-O-Tetradecanoylphorbol 13-acetate (Cat#P8139) and sodium butyrate (Cat#AAA1107906) were purchased from Sigma-Aldrich and Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... and boiled at 95°C for 10 min before electrophoresis on 4-12% pre-cast gels (Sigma) following manufacture instructions ...