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Citations for Millipore Sigma :
1951 - 2000 of 2557 citations for SCAMP1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... For elution of FLAG-tagged proteins and their complexes 100-300 µL of 0.1 mg/mL FLAG3 peptide (Sigma) was added to samples ...
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bioRxiv - Microbiology 2020Quote: ... cells were stimulated with pools of S1 or S2 peptides (final concentration of 2μg/ml) on IPVH-membrane plates (Millipore) coated with 5μg/ml anti-mouse IFN-γ (AN18) ...
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bioRxiv - Physiology 2022Quote: ... 150 μl of 0.75mg/ml myelin oligodendrocyte glycoprotein 33 to 55 peptides (MOG35–55) mixed with Complete Freund’s Adjuvant (FS8810; Sigma-Aldrich) was injected subcutaneously into the tail base of 7 to 11-week-old female C57Bl/6 mice ...
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bioRxiv - Immunology 2022Quote: ... PBMC were restimulated with 1μg/mL peptide + anti-CD28 in the presence of 1μg/ml Brefeldin A (Sigma-Aldrich) for 16h at 37°C ...
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bioRxiv - Bioengineering 2022Quote: ... (Ac)GCRE-ISAFLGIPFAEPPMGPRRFLPPEPKKP(Am) (BM-binder).39 All peptides were reconstituted in acidic (pH 5.5) Milli-Q water (Millipore). The concentration of free thiols in all peptides was determined using Ellman’s reagent (Sigma Aldrich).
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bioRxiv - Pathology 2019Quote: ... For MALDI-TOF/TOF/MS analysis dried peptides were dissolved in peptide resuspension solution (0.1% TFA in 5% ACN) and desalted/concentrated using C18 zip tips (Merck Millipore) as per the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... The peptide concentration was 20 μM in 100 mM N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES) (Sigma Aldrich) buffer (pH 7.4) ...
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bioRxiv - Molecular Biology 2019Quote: ... the remaining resin was washed with 20 mL of A buffer twice and was eluted with 5mL of A buffer containing 0.8 mg/mL 3X Flag peptide (Sigma). The peptide was incubated with the resin for 20 min before elution ...
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bioRxiv - Cancer Biology 2019Quote: ... 0.1% trifluoroacetic acid (TFA) was used to extract peptides and concentrated by C18 zip-tips (Millipore,-C18, P10 size) on Xcise (Proteome Systems) ...
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bioRxiv - Plant Biology 2019Quote: ... and bound protein was eluted with 60 μL of 0.5 mg/mL 3×FLAG peptide (Sigma-Aldrich, MO, USA) for 1 h at 4°C ...
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bioRxiv - Physiology 2019Quote: ... FLAG-tagged protein were eluted twice with 200 ng/μL FLAG peptide (Sigma, #F3290; 1 h at 4°C). PL transfer activity was assayed using NBD labeled Phosphoethanolamine ...
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bioRxiv - Genetics 2020Quote: ... Beads were then washed three times with lysis buffer and eluted in lysis buffer supplemented with 0.5mg/mL 3xFLAG peptide (Sigma). Eluted proteins were precipitated by adding 20% TCA followed by overnight incubation on ice ...
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bioRxiv - Molecular Biology 2020Quote: ... twice with TBS and eluted by incubating samples with Flag elution buffer (200 µg/ml Flag peptide (Sigma-Aldrich) in 1x TBS ...
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bioRxiv - Cell Biology 2020Quote: ... the beads were washed with 1% Trition buffer three times and then incubated in 30 μl of 1% Trition buffer with 0.2 μg/μl of 3xFLAG peptide (Sigma) at room temperature for 20 min ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 mM DTT) and eluted by incubation in TAP buffer supplemented with 0.2 mg/mL HA peptide (Sigma aldrich). Eluates were washed and concentrated by Vivacon 2 ...
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bioRxiv - Immunology 2019Quote: EAE was induced by subcutaneous immunization with 200 μg MOG35–55 peptide (HZI, Braunschweig) emulsified in CFA (Sigma-Aldrich), followed by intravenous injection of 200 ng pertussis toxin (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... Yta7 and mutant versions were eluted in 1 CV of lysis buffer containing 150 mM KCl with 0.5 mg/ml 3x FLAG peptide (Sigma), followed by 2 CV of lysis buffer containing 150 mM KCl with 0.25 mg/ml 3x FLAG peptide ...
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bioRxiv - Microbiology 2020Quote: ... the peptide corresponding to the C-terminus region of BtrS (CALREALRERGYDSVP) were conjugated with hemocyanin from keyhole limpets (Sigma) by using 3-maleimidobenzoic acid N-hydroxysuccinimide ester (Sigma) ...
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bioRxiv - Biochemistry 2021Quote: ... 100 mM NaCl) and eluted with wash buffer containing 0.15 mg/ml 3x FLAG peptide (Sigma cat no. F4799) (Extended Data Fig 1b) ...
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bioRxiv - Immunology 2021Quote: ... and two pools of S2 peptides (pools 3 and 4) (final concentration of 2μg/mL) on IPVH-membrane plates (Millipore) coated with 5μg/mL anti-mouse IFNγ (AN18) ...
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bioRxiv - Biochemistry 2019Quote: ... followed by elution of carboxylase by nutation in 200 μl Buffer A with 100 μg/mL FLAG peptide (Sigma) for 1 h at 4 °C and centrifugation (10000 × g).
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bioRxiv - Genetics 2019Quote: ... the column was inverted and bound proteins were eluted with 10 ml of buffer PT containing 0.1 mg/ml Flag peptide (Sigma). The eluate fractions were dialyzed against buffer A [25 mM Hepes-NaOH (pH 7.5) ...
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bioRxiv - Biochemistry 2021Quote: ... Solvents and reagents used in peptide synthesis were sourced as follows: DMF and DCM (ACS grade) from Sigma-Aldrich; piperidine (99% ...
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bioRxiv - Immunology 2020Quote: ... splenocytes were stimulated with S peptide pools at a final concentration of 2μg/ml on IPVH-membrane plates (Millipore) coated with 5μg/ml anti-mouse IFN-γ (clone AN18 ...
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bioRxiv - Biochemistry 2021Quote: ... sample was placed on a magnetic rack and the supernatant containing peptides was collected and an additional elution (50μl) was performed using 2% dimethyl sulfoxide (DMSO, Sigma) prior sonication in a water bath for 1 min ...
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bioRxiv - Neuroscience 2021Quote: ... were labelled by calcitonin gene-related peptide (CGRP) immunoreactivity using a monoclonal anti-CGRP antibody (1:5000; Sigma #C7113) raised in mouse ...
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bioRxiv - Immunology 2021Quote: ... peptide cocktails were combined with membrane disrupting antimicrobials effective against relevant pathogens beginning at: 10 μM Cecropin A (Sigma), 500μg/mL ampicillin ...
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bioRxiv - Molecular Biology 2021Quote: ... Beads were washed three times with lysis buffer-PBS at 1:4 and eluted with 3xFLAG peptide (Sigma-Aldrich) or Laemmli sample buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... RNCs were eluted after 3 sequential 10 min incubations at room temperature in the RNC buffer that contained 0.2 mg/mL 3x FLAG peptide (Sigma). The elutions were combined and centrifuged at 186,000 x g at 4 °C for 1.5 h in a (TLA-120.1 ...
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bioRxiv - Developmental Biology 2022Quote: ... Beads were then washed three times with lysis buffer and eluted in lysis buffer supplemented with 0.5mg/mL 3xFLAG peptide (Sigma). Eluted proteins were precipitated by adding 20% TCA followed by overnight incubation on ice ...
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bioRxiv - Cell Biology 2022Quote: ... The beads were washed with IP lysis buffer and incubated with 5 mg ml−1 3× FLAG peptide (Sigma–Aldrich ...
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bioRxiv - Immunology 2022Quote: ... at a concentration of 1.5 μg/ml/peptide at 37 °C for 16-18 h in pre-coated ELISpot plate (MSIPS4W10 from Millipore) coated with AN18 IFNγ (1 µg/ml ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... A 20 μL sample of the supernatant and 1 μL of internal peptide standard (H2016, Sigma-Aldrich, Oakville, ON) were added to a 2 mL screw threaded HPLC vial (Chromatographic Specialties ...
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bioRxiv - Plant Biology 2023Quote: ... The washed beads were resuspended in 300 μL wash buffer containing 300 ng/μL of 3xFLAG peptide (F4799, Sigma) and 20 U/mL RNAsin (Promega ...
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bioRxiv - Immunology 2022Quote: ... and 175,000 were co-cultured with 25,000 OTII T cells with 200 ng/mL OVA323-339 peptide (Sigma-Aldrich) and varying ratios of Tregs ...
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bioRxiv - Neuroscience 2022Quote: ... was used in a concentration of 20 µM and autocamtide-2 related inhibitory peptide II (AIP-II; Merck Millipore) at 4 µM.
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bioRxiv - Developmental Biology 2022Quote: ... and peptide peak area ratios were generated against a spiked internal standard with bovine insulin (Sigma – 11070-73-8).
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bioRxiv - Molecular Biology 2024Quote: ... and the FANCI-FANCD2 complex was eluted with FLAG wash buffer containing 100 µg/mL 3X FLAG peptide (Sigma) and 5% glycerol ...
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bioRxiv - Microbiology 2024Quote: ... The antigen-bound magnetic beads were washed five times with lysis buffer and eluted with 2.5 volumes of 3× FLAG peptide (150 ng/μl, Sigma) for 3 h at 4°C with gently shaking ...
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bioRxiv - Plant Biology 2023Quote: ... TF complexes were eluted with 100 μl IP buffer supplemented with 200 μg/ml 3xFLAG peptide (Merck Millipore F4799). The eluted protein was then immobilized on anti-c-Myc magnetic beads (Thermo Fisher 88843 ...
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bioRxiv - Molecular Biology 2023Quote: ... The FLAG-tagged CLAMP proteins were eluted for 3 h at 4°C on a rotating wheel in the presence of 0.5 mg/ml FLAG-Peptide (Sigma) in 1 ml Buffer C containing 1x PIC ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... dried peptides were resuspended in 200 mM ammonium formate adjusted to pH 10 with ammonium hydroxide solution (Sigma-Aldrich). Then ...
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bioRxiv - Molecular Biology 2023Quote: ... The pulldowns were eluted from the FLAG and Strep resins using 0.1 mg/mL 3xFLAG peptide (GlpBio) or 2.5 mM desthiobiotin (Sigma-Aldrich), respectively ...
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bioRxiv - Molecular Biology 2023Quote: ... The FLAG-tagged MSL proteins were eluted for 3 h at 4°C on a rotating wheel in the presence of 0.5 mg/ml FLAG-Peptide (Sigma) in 1 ml Elution buffer ...
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bioRxiv - Cell Biology 2023Quote: ... The resin was then washed with 3x10 bed volumes of lysis buffer and eluted with 800 µL (for 60 ml of cell suspension) lysis buffer (without protease inhibitor) supplemented with 0.2 mg/mL 3x Flag peptides (Sigma). The N-terminal 3xFlag tag was removed by incubation with the GST tagged Prescission Protease (GenScript ...
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bioRxiv - Molecular Biology 2023Quote: ... and 100 µM Na3VO4) before the protein was eluted with FLAG wash buffer containing 0.5 mg/mL 3xFLAG peptide (Sigma). LIMK1 concentration was determined by running alongside BSA standards on SDS-PAGE with Coomassie staining.
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bioRxiv - Molecular Biology 2023Quote: The FLAG-tagged peptide and Pgk1 were detected using the anti-FLAG M2 (Sigma; cat# F1365; 1:1000 dilution) and anti-Pgk1 22C5D8 (abcam ...
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bioRxiv - Biochemistry 2023Quote: ... with molar ratio of 1:5 (Nucleosome:SUV420H1) or 1:100 (Nucleosome: SUV420H1 C-terminal peptide) in the presence of 40 µM of SAM (Sigma). Samples were incubated on ice for 20 mins ...
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bioRxiv - Biophysics 2023Quote: ... The beads were washed 3 × 1 mL with membrane resuspension buffer for KRas before the beads were eluted with membrane resuspension buffer supplemented with 0.2 mg/mL 3X FLAG peptide (SAE0194, Sigma). Aliquots of samples were collected at each stage (input to anti-FLAG resin ...
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bioRxiv - Cell Biology 2023Quote: ... and FLAG-Sox4 protein was eluted by 5 rounds of competitive elution with 1× column volume (0.6 ml) of 100 μg/ml FLAG peptide (Sigma) in TBS ...