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Citations for Millipore Sigma :
2051 - 2100 of 2557 citations for SCAMP1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in primary antibody diluted in blocking solution (PBST, 1% BSA) overnight at 4°C with the following primary antibodies: mouse anti-NeuN (1:500, Millipore, Mab377), rat anti-BCL11B (1:1,000 ...
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bioRxiv - Microbiology 2022Quote: ... Tissue sections were then permeabilized overnight at 4°C by gently rocking in blocking buffer comprising 3% bovine serum albumin (Sigma Aldrich), 1% saponin (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... blocked in Pure Odyssey Blocking Buffer (Li-COR Biosciences, Lincoln) for 2 hours and incubated with an anti-ABCA4 clone 5B4 (1:1000; Sigma-Aldricht) and anti-Vinculin (1:5000 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with primary antibodies in blocking buffer for 1h at 21°C: 1:1000 α-tubulin (118M4779V; Sigma Aldrich), 1:250 CREST (15-234-0001 ...
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bioRxiv - Immunology 2022Quote: ... Fixed cells were washed twice with PBS and the remaining PFA was inactivated with blocking buffer (PBS-2% BSA (Sigma: #A9418), 1.5M Glycine (Sigma ...
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bioRxiv - Pathology 2020Quote: ... The blocking solution used for the anti-phospho-Smad2 and anti-phospho-Smad3 antibodies contained 5% bovine serum albumin (Sigma-Aldrich). The primary antibodies applied to HK-2 cells were anti-α-smooth muscle actin (α-SMA ...
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bioRxiv - Neuroscience 2020Quote: ... after several washes in PBS explants were incubated at room temperature for 1 hour in secondary antibodies diluted in the blocking solution (at 1:500 for Alexa-coupled antibodies (Life Sciences)) and 1:400 for TRITC-conjugated phalloidin (Sigma-Aldrich)) and then mounted using Fluoromount-G (Southern Biotech).
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bioRxiv - Biophysics 2021Quote: ... cell monolayers were allowed to migrate for at least four hours before exchanging the medium with pre-warmed medium containing 10 μg/ml E-cadherin blocking antibody (clone DECMA-1, Millipore MABT26).
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bioRxiv - Immunology 2020Quote: ... The IHC protocol mirrors the MIBI-TOF protocol with the addition of blocking endogenous peroxidase activity with 3% H2O2 (Sigma Aldrich) in ddH2O after epitope retrieval ...
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bioRxiv - Genomics 2019Quote: ... Cells were incubated with primary antibody diluted in the blocking buffer for 2 hours [alpha-tubulin mouse mAb (1:5000, Sigma T6199), Rad21 rabbit pAb (1:1000 ...
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bioRxiv - Genetics 2020Quote: ... 50 mL of blocking buffer was prepared per 2×108 cells by adding 1 gram of biotin-free BSA (Sigma Aldrich) and 200 μl of 0.5 M pH 8.0 EDTA (ThemoFisher 15575020 ...
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bioRxiv - Cell Biology 2020Quote: ... the channel was washed three times with 10 μl nuclease-free water and the channel walls were passivated with 10 mg/ml Western Blocking Reagent (Sigma Aldrich) in PBS for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were subsequently incubated for 1 hr at room temperature in a blocking solution consisting of PBS with 5% Goat Serum (Sigma-Aldrich). Following blocking ...
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bioRxiv - Biochemistry 2021Quote: ... Magnetic protein G beads were washed with blocking solution (0.5% BSA in PBS) and then incubated with rabbit antiserum against acetylated histone H4 (Millipore 06-866) or normal rabbit anti-serum in blocking solution for several hours at 4°C with rotation ...
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bioRxiv - Cancer Biology 2020Quote: ... membranes were blocked in blocking buffer (5% skim milk, 0.1% Tween in PBS) and incubated with β-Actin (#A5316, Sigma-Aldrich), MYC (#9402 ...
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bioRxiv - Cell Biology 2021Quote: ... the proportions of respiration driving ATP synthesis and proton leak were determined by blocking ATP synthase with 2 μM oligomycin (Sigma-Aldrich). Subsequently ...
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bioRxiv - Genomics 2021Quote: ... The cells were rinsed and kept in the blocking buffer before photo-bleaching and submerged in 3-5’-diaminobenzidine (DAB) solution (Sigma Aldrich) during photo-bleaching on the cold stage.
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bioRxiv - Neuroscience 2020Quote: ... Tissues were then incubated overnight with the following cocktail of primary antibodies to presynaptic components diluted in blocking solution with rocking: rabbit anti-NF 200 (1:200; Sigma, N4142), mouse anti-synaptophysin (1:300 ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated with blocking and permeabilization solution (PBS containing 0.25% Triton-X100 (Cat#93443, Sigma-Aldrich, St Louis, MO, USA) and 3% bovin serum albumin (BSA ...
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bioRxiv - Microbiology 2022Quote: Fixation was carried on in 4% PFA for 15 minutes and then the coverslips where incubated with a blocking/permeabilization solution (PBS-BSA 1%, 2mM Vanadyl ribonucleoside complexes (VRCs) solution (Sigma #94742), TRITON-X 0.3% ...
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bioRxiv - Immunology 2022Quote: ... Sections were then cleared using 10% w/v CHAPS and 25% v/v N-Methyldiethanoamine in PBS for 48hrs before washing with PBS followed by 72hrs of blocking 5% donkey serum (Sigma, #D9663) in PBS ...
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bioRxiv - Systems Biology 2022Quote: ... 50 mL of blocking buffer was prepared per 2 × 108 cells by adding 1 g of biotin-free BSA (Sigma Aldrich) and 200 mL of 0.5 M pH 8.0 EDTA into DPBS (GIBCO) ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were incubated for 30 min in blocking solution (0.03% v/v Triton X-100 [T8787, Sigma], 3% v/v bovine serum albumin [A9418, Sigma], in PBS) to improve antibody binding specificity ...
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bioRxiv - Neuroscience 2022Quote: ... Multiplex antibody cocktails were made in a whole-mount blocking solution consisting of 5% heat activated normal donkey serum (Sigma Millipore), 20% Dimethyl sulfoxide (Sigma Millipore) ...
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bioRxiv - Neuroscience 2022Quote: ... VTA brain sections were incubated for 1 hour at 4°C in a blocking solution of phosphate-buffered saline (PBS) containing 3% bovine serum albumin (BSA, Sigma; A4503) (vol/vol ...
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bioRxiv - Neuroscience 2022Quote: ... VTA brain sections were incubated for 1 hour at 4°C in a blocking solution of phosphate-buffered saline (PBS) containing 3% bovine serum albumin (BSA, Sigma; A4503) (vol/vol ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were incubated with the corresponding primary antibodies diluted in blocking solution: anti-tyrosine hydroxylase (TH) (Millipore, #MAB318; 1:2000) or anti-NeuN (Millipore ...
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bioRxiv - Cell Biology 2022Quote: ... #T9284)/PBS (15 minutes), washed in 1× PBS, and incubated (2 hours) with blocking solution (2% BSA, 5% donkey serum (SIGMA, #D9663), and 0.3% Triton X-100/PBS) ...
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bioRxiv - Microbiology 2024Quote: ... and mouse anti-ferret totIg (mAb 8H9 + mAb 4B10) diluted each to 1 µg/mL in blocking buffer (PBS containing 2% BSA [Sigma-Aldrich] ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were blocked with 1 drop of blocking solution provided with the Duolink® PLA Probes (DUO92002 and DUO92004, Sigma-Aldrich) for 1h at 37°C in a humid chamber ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated in blocking buffer containing secondary antibodies and 0.5 μg/mL 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich #D9542) for one hour ...
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bioRxiv - Molecular Biology 2024Quote: ... The tumours were then permeabilised in 70% EtOH for 2 h at RT and then blocked with blocking buffer [1% BSA (Sigma Aldrich), 0.2% Triton-X (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... Overnight primary antibody incubation was performed on a rocker in blocking solution containing the Anti-Tyrosine Hydroxylase (1/1000, Millipore, AB152) antibody ...
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bioRxiv - Genetics 2023Quote: ... The next morning membranes were washed once with blocking buffer for 5-10min and incubated with horseradish peroxidase-conjugated secondary antibodies (Sigma-Aldrich) diluted 1:10 000 in blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µl of a blocking/permeabilization buffer (5% BSA and 0.2% Triton in PBS; BSA from Sigma; TritonX-100 from VWR) was added and cells were incubated for 1h at RT ...
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bioRxiv - Systems Biology 2023Quote: ... Thereafter the blocking buffer was discarded and the membranes were incubated with 1:1000 dilutions of mouse anti-FLAG M2 antibody (Sigma-Aldrich) in 1X TBST (overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... They were then incubated in primary antibodies (supplementary table 7) diluted in Odyssey blocking buffer supplemented with 0.2% (v/v) Tween 20 (Sigma-Aldrich, P9416) overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... GFP-Trap®_MA beads were washed with low-salt buffer and blocked for 60 mins at room temperature in Casein Blocking Buffer (Sigma-Aldrich) supplemented with 50 μg/mL Heparin ...
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bioRxiv - Biophysics 2023Quote: ... we added a 1:400 solution of primary antibody in blocking buffer (anti-GM130 ab52649 Abcam, anti-tubulin Millipore Sigma T6199). We incubated coverslips with the primary antibody overnight at 4 °C ...
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bioRxiv - Biophysics 2023Quote: ... We blocked the membrane for 1 hour with blocking buffer: 5% bovine serum albumin (BSA, BioShop) in tris-buffered saline (BioShop) with Tween 20 (Millipore Sigma) (TBST ...
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bioRxiv - Microbiology 2023Quote: ... in PBS 1X at RT for 40 min and incubated with a blocking buffer containing 3% bovine serum albumin (BSA, Sigma Aldrich) in PBS 1X RT for 40 min ...
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bioRxiv - Neuroscience 2023Quote: ... preparations were washed for 1 hour in blocking buffer containing 5% (vol/vol) normal goat serum (NGS) (Cat# G9023, Sigma-Aldrich) in PBT then incubated for 24–48 hr at 4 °C in the primary antibody mouse anti-GFP (1:500 in blocking buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... After blocking in LiCOR blocking buffer the following primary antibodies were used: DDX5 (Pab204, gift from Prof. Frances Fuller-Pace; clone204, Sigma-Aldrich), DHX9 (ab26271 ...
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Molecular identification of ALDH1A1 and SIRT2 in the astrocytic putrescine-to-GABA metabolic pathwaybioRxiv - Neuroscience 2023Quote: ... The cells were then incubated with primary antibodies in blocking solution in the following composition: guinea-pig anti-GABA antibody (1:1000, AB175, Millipore, USA), chicken anti-GFAP antibody (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... Embryos were washed multiple times with PBST (0.8% Triton-X 100 in PBS) and incubated in blocking buffer (10% NGS (Sigma-Aldrich, #G6767) and 4% BSA (Sigma Aldrich ...
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bioRxiv - Physiology 2023Quote: ... and then incubated overnight at 4°C in primary antibodies diluted in 50% blocking buffer / 50% Tris buffered saline (bioWORLD #42020056–3) + 0.1% TWEEN (Sigma-Aldrich #P9416) (TBS-T) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells where thrice washed in PBS then incubated in blocking buffer (5% Donkey Serum (Jackson Immuno Research Labs 017-000-121, 1% BSA (Sigma A9647), 0.1% Triton X-100 (Sigma T8787 ...
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bioRxiv - Immunology 2023Quote: ... Blocking was performed in 5% non-fat dry milk in PBS containing 0.2% Tween 20 (Sigma-Aldrich, St. Louis, Missouri, USA). Membranes were incubated in primary antibodies for 1-3 h at room temperature (20-25°C ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were subsequently blocked for 1 hour at room temperature in blocking buffer (PBS with 5% skimmed milk powder (Sigma-Aldrich) and 0.1% Tween) ...
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bioRxiv - Cell Biology 2023Quote: ... After blocking, cells were incubated with anti-GLUT4 (LM048) (2 µg/mL, Integral Molecular, PA, USA) and Lectin-FITC conjugate (Sigma #L4895) for 1 h at room temperature ...