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Citations for Millipore Sigma :
2101 - 2150 of 2557 citations for SCAMP1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Specimens were washed with phosphate-buffered saline and blocking solution (1% BSA, 0.1% gelatin from cold-water fish skin (Sigma-Aldrich G7041), 0.5% Triton X-100 and 0.05% Tween-20 (Sigma-Aldrich P1379 ...
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bioRxiv - Developmental Biology 2023Quote: ... the slides were rinsed three times with PBS and blocked for 1 hour at RT in blocking buffer made with 3% BSA (Merck Millipore, 0218072801) and 0.02% Triton X-100 (Sigma ...
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bioRxiv - Developmental Biology 2023Quote: ... for 30 minutes at room temperature and then overnight at 4°C in blocking solution containing mouse anti-Alpha-tubulin (1:500, Sigma, #T9026). Next ...
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bioRxiv - Cancer Biology 2023Quote: ... cleared biopsies were blocked for 3–4 h at room temperature (RT) or overnight at 4°C in blocking buffer (PBS (Sigma-Aldrich), 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: ... PLUS and MINUS PLA probes were diluted in the Duolink Antibody diluent in 1:5 ratio with blocking buffer from the PLA kit (Millipore Sigma). The coverslips were then incubated in PLA probe solution for 1 hour at 37°C in humidity chamber ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were incubated with PBG blocking buffer containing secondary antibodies and 0.4 μg/ml DAPI (4’,6-diaminido-2-phenylindole, Sigma, cat # D9542) for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... cells were washed once with PBS for 10 min followed by a blocking- permeabilization step for 1 hr at RT in blocking buffer (PBS/ 5% donkey serum Bio-Rad C06SB / 0.1% Triton Sigma X100). Primary antibodies diluted in blocking buffer were then added to the cells (Table 1 ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were kept in the blocking solution (5 % Normal Goat Serum, 0.1 % Triton-X, 0,1 % DMSO in PBS) (Sigma Life Science, 1002135493) for at least 1 hour before incubating in the primary AB containing-blocking solution overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies were diluted in blocking reagent to detect Pax7 (Developmental Studies Hybridoma Bank, 1:10) and laminin (Sigma, 1:50). After 16h incubation at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos after in situ HCR were washed with PBST and soaked in blocking solution [5% heat inactivated (56°C, 30 min) sheep serum (S2263, Sigma-Aldrich) in PBST] at room temperature for 2 hours ...
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bioRxiv - Neuroscience 2023Quote: ... Free-floating brain sections were then incubated for 1 hour at 4C in a blocking solution of phosphate-buffered saline (PBS) containing 3% bovine serum albumin (BSA, Sigma; A4503) (vol/vol ...
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bioRxiv - Bioengineering 2023Quote: ... followed by 1-hour blocking of non-specific binding sites with PBS with 5%v/v bovine serum albumin (BSA) (Sigma Aldrich) at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... membranes were incubated ON at 4 °C with primary antibodies diluted in blocking solution supplemented in 0.05 % Tween-20 (Sigma-Aldrich Corp.); anti-HSP70 (1:500 ...
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bioRxiv - Developmental Biology 2023Quote: ... Gradual conversion of each sample to 1XPBT was performed using a diluted series of SSC/PBT and samples were blocked in blocking buffer for 3 hours at room temperature before adding 1:10000 of anti-DIG antibody (Millipore/Sigma) overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Once dried, coverslips were placed upside down on a drop of hybridizing solution (70% formamide, 0.5% blocking reagent (Sigma, Cat:11096176001), 10 mM Tris-HCl pH 7.2 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with homemade anti-RBM20 (1:400) primary antibody in blocking buffer [PBS containing 5% goat serum (cat# G6767; Sigma-Aldrich), 0.1% Triton X-100 (cat# X100 ...
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bioRxiv - Cell Biology 2022Quote: ... and then incubated with primary antibodies in blocking buffer [1:400 homemade rabbit anti-RBM20,15 or 1:500 anti-α-actinin (cat# A7811; Sigma-Aldrich)] overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... and then incubation overnight at 4°C in blocking solution with primary antibodies: mouse anti-CRE (Sigma, F3165-2MG, 1:1000); mouse anti-Luciferase (Sigma ...
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bioRxiv - Bioengineering 2023Quote: ... collagen and mineralized collagen in the presence and absence of a function-blocking β1 integrin antibody (2 µg/mL, Millipore, US) or pharmacological inhibitors of focal adhesion kinase (FAK ...
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bioRxiv - Developmental Biology 2023Quote: ... After washing 3x in ddH20 slides were blocked for 1 hour with blocking solution: 5% normal donkey serum (Sigma, Cat#D9663), 0.1% Tween-20 in PBS ...
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bioRxiv - Systems Biology 2023Quote: ... 50 mL of blocking buffer was prepared per 2 x 108 cells by adding 1 g of biotin-free BSA (Sigma Aldrich) and 200 mL of 0.5 M pH 8.0 EDTA into DPBS (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... followed by permeabilization and blocking for 1 hour at room temperature in PBS with 0.5% Triton-X-100 (Sigma #X100-500ML)/0.2% Tween-20 (Sigma #P1379)/2% BSA (Sigma #A7906) ...
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bioRxiv - Neuroscience 2023Quote: ... brain sections were washed three times for 15 min with 1X TBS and then incubated for 1.5 h in blocking solution: 2.5% Donkey Serum (Sigma, Merck, Germany, S30); 2.5% Goat Serum (Sigma ...
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bioRxiv - Physiology 2023Quote: ... the secondary antibody was applied by incubating the slides for 1 hour at room temperature with blocking buffer containing Alexa Fluor 647-conjugated goat anti-mouse antibody (1:1000, Sigma, SAB4600354). The slides were then briefly rinsed in PBS-Tw before a 10-minute incubation in PBS-Tw containing 1:2000 SYTOX Orange (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... Free-floating brain sections were then incubated for 1 hour at 4C in a blocking solution of phosphate-buffered saline (PBS) containing 3% bovine serum albumin (BSA, Sigma; A4503) (vol/vol ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated with anti-calbindin-D28k mouse monoclonal antibody (1:2000 dilution by the blocking solution, C9848, Millipore Sigma) at 4 °C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... Coverslips were washed twice more with 0.01M PBS before blocking in 5% (v/v) normal donkey serum (NDS; catalogue # S30-100ml, Sigma Aldrich, UK) in 0.01M PBS (3% Tween-20 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then blocked and permeabilized in protein-blocking buffer (DPBS with 2% BSA, 10% fish gelatin (cat#: G7041, Sigma-Aldrich), 0.2% Triton-X (cat# T9284 ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were then incubated overnight at 4°C with the primary antibody diluted in blocking solution (5% goat and/or donkey serum in PBS, #G9023 and #D9663 Sigma-Aldrich). Cells were washed with complete PBS 3 times for 5min at RT and then incubated with the following secondary antibodies for 1h at RT ...
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bioRxiv - Bioengineering 2023Quote: ... The membrane was cut at 25 kDa to separate actin and LC3 bands and primary antibody in 5% BSA blocking buffer (anti-LC3B, rabbit, Sigma-Aldrich L7543 at 1:1000 and anti-actin ...
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bioRxiv - Immunology 2023Quote: ... in PBS for at least 30 min at 37 °C and permeabilized with PBS containing 0.1 % Triton-X for 5 minutes and blocked with blocking solution (3 % BSA in PBS) at RT for 30 minutes (all reagents from Sigma-Aldrich). Then ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were incubated with the following primary antibodies diluted in blocking buffer overnight at 4°C: mouse anti-FLAG M2 (1:500; Sigma; F1804), rabbit anti-PEX14 (1:500 ...
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bioRxiv - Developmental Biology 2023Quote: ... all samples were incubated with Blocking One Histo (Nacalai Tesque) for 10 min at RT and 10% normal donkey serum (Sigma-Aldrich) in PBS/0.5% Triton X-100 with 5% Blocking One Histo for 30 min at RT.
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bioRxiv - Cell Biology 2024Quote: ... Fixed cells were then incubated for 30 min at 37°C in blocking buffer [0.5% (w/v) bovine serum albumin (BSA, Sigma Cat. #A9647), 10% (w/v ...
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bioRxiv - Immunology 2024Quote: ... Cultures were washed with 4% FBS in PBS and incubated with blocking solution (2% FBS, 2% bovine serum albumin [BSA, A2153; Sigma], and 0.1% Tween-20 [P9416; Sigma] in PBS) for 45 min ...
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bioRxiv - Biochemistry 2024Quote: ... then incubated for 1-2 h at room temperature in PBST with blocking agent (5 mg/mL Bovine Serum Albumin (Sigma Aldrich) for anti-Strep blots ...
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bioRxiv - Immunology 2021Quote: ... The mix was transferred to a plate coated with avidin and the FLAG-peptide was detected with an anti-FLAG-alkaline phosphatase conjugate (Sigma), developed with pNPP SigmaFAST substrate and absorbance was read with a 405nm – filter (Figure 3D-F).
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bioRxiv - Molecular Biology 2021Quote: ... The beads were then washed three times for 5 min with mild EJC-Buffer before eluting twice with 22 μl of a 200 μg/ml dilution of FLAG-peptides (Sigma) in 1x TBS for 10 min at RT and 200 rpm each elution step ...
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bioRxiv - Neuroscience 2020Quote: ... mice were administered murine MOG 35-55 (Johns Hopkins Peptide Synthesis Core Facility, Baltimore) in incomplete Freund’s adjuvant (Sigma-Aldrich) supplemented with heat-killed mycobacterium tuberculosis (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... encoding PPK with a C-terminal GAAEPEA peptide tag for affinity purification (105) between the NdeI and HindIII sites of plasmid pET-21b(+)(Novagen), was synthesized by GenScript.
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bioRxiv - Molecular Biology 2020Quote: ... was replaced by the wild-type CARD14 gene obtained from pFN21AE3344 with N-terminal 3xFLAG tag peptides (DYKDHDGDYKDHDIDYKDDDDK) (Sigma-Aldrich). To generate CARD14-expressing vector (pTRE3G-Pur-3FLAG-CARD14wt ...
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bioRxiv - Developmental Biology 2022Quote: A peptide corresponding to residues 1 – 159 of HyYap was expressed as GST-fusions using the GEX4t-1 vector (Millipore) in E ...
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bioRxiv - Immunology 2022Quote: ... stimulated with SARS-CoV-2 spike protein overlapping peptide library or stimulated with 5μg/ml of Phytohaemagglutinin (PHA) (Sigma-Aldrich) as a positive control ...
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bioRxiv - Immunology 2021Quote: ... The beads were then washed with IP buffer three times and incubated in FLAG-elution buffer (100 μg/mL FLAG peptides (Sigma), 50 mM Tris-HCl pH7.4 ...
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bioRxiv - Molecular Biology 2021Quote: ... and proteins were eluted in Laemmli buffer by boiling at 95°C for 5min for Western blot or with 3xFlag peptide (150 µg/ml, Sigma) for 1 hr at 4°C for HAT assay ...
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bioRxiv - Molecular Biology 2019Quote: ... for 2 hours at 4°(C) Immunocomplexes were washed 5× with S300 buffer and eluted by competition with 1x Flag peptide (500 ng/ul; SIGMA) 2 times for 30 min at 16 °C and then resuspended in Laemmli sample buffer ...
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bioRxiv - Immunology 2019Quote: ... Commercial sources were used to obtain biotinylated CD16 (Acro Biosciences) and the biotinylated 19-mer adjacent loop peptides that overlap by 16 amino acids (Sigma). Loop peptide 1 is KDQQLLGIWGCSGKLICTT and loop peptide 2 is QLLGIWGCSGKLICTTAVP.
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bioRxiv - Immunology 2020Quote: ... Beads were eluted three times at room temperature for 15 min each using Influenza Hemagglutinin (HA) peptide (Sigma Aldrich I2149).
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bioRxiv - Cell Biology 2019Quote: ... Beads were washed extensively in lysis buffer and bound proteins eluted in 500 µL 100 µg/ml 3X FLAG peptide (Sigma) in 25 mM Tris-HCl pH 7.4 ...
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bioRxiv - Cancer Biology 2020Quote: ... were plated in 96-well plates (flat bottom, Costar) and pulsed with the indicated amounts of Melan-A peptides (ELAGIGILTV, Sigma) for 2 hours at 37C ...