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Citations for Millipore Sigma :
1751 - 1800 of 2557 citations for SCAMP1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Core complexes were eluted from anti-Flag resin with Flag buffer containing 250 μg/ml 3xFlag peptide (Sigma). Fractions containing protein were pooled and loaded on a Superdex 200 Increase 10/300 GL column (Cytiva ...
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bioRxiv - Microbiology 2024Quote: ... beads were resuspended in 35 µL TBS (50 mM Tris-HCl, 150 mM NaCl, pH 7.5) with 0.5 µg/µL FLAG peptide (Sigma) and incubated at room temperature for 15 min at 900 rpm ...
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bioRxiv - Biophysics 2024Quote: ... The Aβ (1–42) peptide (Stratech, A-1163, 0.5 mg) was initially dissolved in 100% HFIP (Sigma-Aldrich) at a concentration of μM ...
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bioRxiv - Microbiology 2024Quote: ... Stock solutions (1 mg/ml) were prepared by dissolving the peptides in 0.1% trifluoroacetic acid (TFA) (Sigma, Germany). The mass correctness and absence of degradation of the synthetic peptides were verified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS ...
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bioRxiv - Cell Biology 2024Quote: ... Elution was done by adding either 20 μl of 0.2 mg/ml of 3xFLAG peptide (Sigma-Aldrich F4799) or 20 μl of 1x Strep-Tactin Elution Buffer (IBA 2-1042-025 ...
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bioRxiv - Microbiology 2024Quote: Fifteen amino acid (aa) peptides overlapping 12 aa spanning the structural proteins of CHIKV were purchased from Sigma and were divided into 4 overlapping peptide pools ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were incubated with primary antibodies (Table 3) diluted in Intercept (TBS) blocking buffer with 0.05% Tween-20 (Sigma-Aldrich) overnight at 4°C on an orbital shaker.
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were blocked with either Odyssey Blocking Buffer (Licor Cat #927-40000) or 5% skimmed milk in TBST buffer (SIGMA) and incubated for 1h at room temperature or overnight at 4° ...
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bioRxiv - Cell Biology 2020Quote: ... These slides were rehydrated in 1x PBS at room temperature for 15 min followed by blocking for 1 hour in 1x PBS containing 3% BSA (Bovine serum albumin, Sigma). The slides were then incubated for 1 hour at room temperature with 1xPBS+3% BSA with respective antibodies ...
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bioRxiv - Developmental Biology 2021Quote: ... the embryos were incubated overnight with an AP-coupled anti-DIG antibody in the blocking solution and finally stained with NBT/BCIP liquid substrate (Sigma). After the staining ...
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bioRxiv - Developmental Biology 2021Quote: Newborn pups were injected at P2 via the temporal vein with 0.0025mg/g of DLL4-neutralizing (blocking antibody (YW152F, Genentech (31)) or IgG from human serum (cat #I4506, Sigma Aldrich). The pups were sacrificed 48 h after injection at P4 ...
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bioRxiv - Biophysics 2020Quote: ... The membrane was incubated for 1 h in a blocking buffer of PBST (PBS supplemented with 0.1 v/v % Tween) and 10 % (v/w) dry skim milk (Sigma-Aldrich). The membrane was washed 3 times with PBST for 5 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were permeabilized with 0.5 % TritonX-100 (Wako) in 1x PBS for 1-2 h and blocked in blocking solution (2 % BSA (Sigma-Aldrich), 1 % DMSO ...
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bioRxiv - Developmental Biology 2021Quote: ... Rigid paralysis of the skeletal muscles was induced by treatment with the neuromuscular blocking agent decamethonium bromide (DMB) (Sigma Aldrich) (Bowman and Rand ...
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bioRxiv - Plant Biology 2022Quote: ... following an hour of primary antibody incubation in Intercept® (PBS) Blocking Buffer with 0.2% Tween® 20 (Sigma, P1379) at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... the samples were permeabilized with TritonX-100 at 0.1% in D-PBS with subsequent blocking with 3% Bovine Serum Albumin (Sigma-Aldrich) in D-PBS ...
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bioRxiv - Neuroscience 2021Quote: ... For immunofluorescence stainings slices were incubated for 1 h at room temperature (RT) in blocking buffer (2% gelatine, 2% BSA (bovine serum albumin, Cat#a2153, CAS Number: 9048-46-8, Sigma), 0.1% Triton X-100 (Merck ...
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bioRxiv - Neuroscience 2021Quote: ... Whole brains were dissected and incubated in blocking solution (5% normal donkey serum and 1% Bovine Serum Albumin (BSA; Sigma) in PBS with 0.1% Triton-X100 (PBS-T) ...
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bioRxiv - Immunology 2021Quote: ... for 10 min at room temperature followed by 1 h blocking step with PBS containing 0.4% Triton X-100 5% goat serum (all Sigma-Aldrich) before incubation overnight at 4°C with anti-Muc2 (Novus Biologicals #NBP1-31231 ...
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bioRxiv - Immunology 2019Quote: ... saturated in blocking solution (PBS-BSA 5%) for 1 hour and finally incubated with primary Abs: rabbit anti-mouse laminin (Sigma), Alexa fluor 594 Goat anti-mouse IgM (Thermofisher ...
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bioRxiv - Physiology 2020Quote: ... followed by antigen retrieval in citrate buffer (10 mM, pH=6.0) for 40 min in 90□ and blocking in 2% BSA (A2153, Sigma Aldrich) with 0,3 M glycine (G8898 ...
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bioRxiv - Immunology 2019Quote: ... This was followed by incubation for 1 hour at room temperature in blocking buffer consisting of 2% goat serum (Sigma) in PBS 0.1% Triton X-100 ...
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bioRxiv - Developmental Biology 2019Quote: ... Antibody and chemical staining were performed in blocking solution at the following dilutions: anti-β-catenin 1:500 (Sigma C2206), anti-laminin 1:500 (Sigma L9393) ...
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bioRxiv - Neuroscience 2019Quote: Tissue sections were washed in PBS and subsequently blocked for 3 h at room temperature in blocking buffer (PBS containing 0.2% Triton X-100 (Sigma-Aldrich) and 5% goat serum (Gibco)) ...
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bioRxiv - Microbiology 2019Quote: ... were first floated on ultrapure water for 5 min before incubation in blocking buffer (1xPBS containing 0.1%v/v Tween-20 and 3% fatty-acid free BSA (Sigma A7030)) at RT for 1h ...
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bioRxiv - Genomics 2019Quote: ... Cells were then incubated with a STAT3 antibody (9139, Cell Signalling) in blocking buffer + 0.1% tween-20 (P1379, Sigma Aldrich) for 1-2 hours at room temperature or overnight at 4°C on a shaker ...
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bioRxiv - Neuroscience 2019Quote: ... sections were incubated with 0.5% Triton and blocked with 0.5% Triton and goat serum blocking solution before being incubated in mouse anti-PV monoclonal antibody (1:2000, Sigma-Aldrich) and mouse anti-NeuN polyclonal antibody (1:2000 ...
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bioRxiv - Molecular Biology 2020Quote: Sections were incubator in PBS for 10 min and then blocked with blocking buffer (5% Bovine Serum Albumin (BSA, Sigma) in PBS with 0.1% Triton X-100) ...
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bioRxiv - Neuroscience 2020Quote: ... Spontaneous IPSCs were isolated by blocking AMPA receptor-mediated events with CNQX (6-cyano-7-nitroquinoxaline-2,3-dione; 20uM; Sigma-Aldrich). Miniature events were isolated by blocking sodium channels with the addition of tetrodotoxin (1uM ...
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bioRxiv - Bioengineering 2021Quote: ... then incubated in blocking buffer containing 1% (w/v) BSA and 10% (v/v) goat serum (all from Sigma-Aldrich) in 1X PBS for 1 hour at room temperature (RT ...
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bioRxiv - Neuroscience 2020Quote: ... were coated for 2h with 5.0 µg/ml monoclonal GR antibody followed by overnight blocking with 1% BSA in PBS-Tween (0.05% Tween-20, Sigma Aldrich) at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... The blocking buffer was next replaced with rabbit polyclonal T22 antibody that recognises oligomeric Tau (diluted 1/2000) ((ABN454; Merck Millipore) (37) ...
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bioRxiv - Neuroscience 2021Quote: ... for 30 min prior to incubation for 1 h in primary antibodies diluted in blocking buffer: Goat anti-mouse GAPDH (RRID: AB_1078991, Sigma-Aldrich) 1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were then blocked and permeabilized using a DPBS-based blocking buffer containing 0.1% (w/w) saponin (Sigma-Aldrich, S4521) and 5% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... then incubated with a 0.5% Triton X-100 solution and finally with casein blocking buffer (0.25% casein (Sigma, C3400-500G) in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated overnight at 4°C in blocking buffer with the following primary antibodies: rat anti-MBP (monoclonal, 1:100, Millipore), rabbit anti-Parvalbumin (1:500 ...
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bioRxiv - Plant Biology 2021Quote: ... After blocking with Tris Buffered Saline with Tween 20 (TBST) containing 5% (w/v) bovine serum albumin (BSA) (Sigma, A3608) for 1 h ...
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Induction of Dopaminergic Neurons for Neuronal Subtype-Specific Modeling of Psychiatric Disease RiskbioRxiv - Neuroscience 2021Quote: ... 500uL of a blocking solution consisting of PBS with 5% donkey serum (Jackson, #017-000-121) and 0.1% Triton X-100 (Sigma, #T8787) was added to the wells and incubated for one hour at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was blocked in the blocking buffer of 5% BSA in TBST (137 mM NaCl, 2.7 mM KCl [Sigma-Aldrich P9541] ...
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bioRxiv - Cancer Biology 2021Quote: ... The chambers were gently washed twice with PBS and filled with 100μL of blocking solution - 2.5% (w/v) bovine serum albumin (Sigma-Aldrich, A9418) in PBS - and incubated for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then blocked and permeabilized for 30 min at RT in blocking buffer that contained 3% bovine serum albumin (BSA) and 0.1% Triton X-100 (Sigma Aldrich). Subsequently ...
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bioRxiv - Physiology 2022Quote: ... Cells were washed with PBS three times for 5 min and incubated with blocking buffer consisting of 5% goat serum (CST) and 0.3% Triton X-100 (Sigma Aldrich) in PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... permeabilized for 30 min at room temperature in DPBS/0.3% Triton-X100 and block by blocking buffer (PBST containing 3% donkey serum (Sigma, D9663) and 1% BSA (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by blocking for 1 hour at room temperature with 4% skim milk powder (Sigma-Aldrich, St. Louis, MO, USA) in Tris-buffered saline pH 7.4 (TBS ...
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bioRxiv - Molecular Biology 2020Quote: ... The primary antibody was then replaced with blocking solution containing 5 μg/mL bisBenzimide H 33342 trihydrochloride (Sigma-Aldrich, B2261) and goat anti-mouse Alexa Fluor 488 secondary antibody (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... washed 3 times with PBS and incubated for 2 h with blocking and permeabilization buffer (1% BSA, 5% goat serum, 0.15% saponin (Sigma Aldrich)) ...
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bioRxiv - Neuroscience 2021Quote: ... at 40um thickness from postmortem human brain blocked for 1 hour at room temperature in blocking buffer before being incubated in primary antibodies for 72 hours at 4°C with gentle rocking in blocking buffer (Anti-NeuN, Synaptic Systems cat no. 266 004, 1:500; Anti-phospho-Histone H2A.X, Millipore cat no ...
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bioRxiv - Neuroscience 2021Quote: ... and APC rat anti-mouse 4D4 (marking resident microglia; 1:1000) in blocking buffer containing 0.2% bovine serum albumin (BSA, Sigma-Aldrich) in HBSS ...
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bioRxiv - Neuroscience 2020Quote: ... cells were washed in PBS before permeabilisation in 0.1% Triton X-100 (Fisher Bioreagents) and blocking in 1% bovine serum albumin (BSA; Fisher Bioreagents) and 10% normal goat serum (NGS; Sigma) in PBS for 30 min ...
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bioRxiv - Biophysics 2021Quote: ... cells were treated for 10 min with a solution of 0.25% Triton-X100 in blocking buffer (solution of 3% bovine serum albumin (BSA, Sigma-Aldrich) in BRB80 buffer) ...