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Citations for Millipore Sigma :
1801 - 1850 of 10000+ citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... the solubilisate was precleared by incubation with 15 μg bead-immobilized control IgG (Millipore 12-370) for 1 h at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... OSU-ERb-12 was administered as a solution in 20% hydroxypropyl-β-cyclodextrin (HPBCD) (Sigma H107). Tamoxifen (Sigma T5648) ...
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bioRxiv - Cancer Biology 2024Quote: 12-well cell culture plates were coated with 50 µg/ml poly-D-lysine (Sigma-Aldrich) for 1 hour ...
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bioRxiv - Biochemistry 2024Quote: 12 to 14-week-old mice were treated with antibiotic mixture mixture (chloramphenicol (Sigma, C0378-25G), spectinomycin dihydrochloride pentahydrate (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... dialysis was performed for 12 h against the buffer A containing 50% glycerol (Sigma-Aldrich, USA). The His-tag of the recombinant protein was removed during 12 h of incubation with 1-2 units of the enteropeptidase (L-HEP ...
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bioRxiv - Immunology 2024Quote: ... Stimulation included phorbol-12-myristat-13-acetat (PMA, Sigma-Aldrich; #P8139- 5M6, concentration of 2ng/µl) and ionomycin (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... The cells were exposed to phorbol 12-myristate 13-acetate (PMA, 50 ng/ml, Sigma-Aldrich) and ionomycin (1 μM ...
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bioRxiv - Synthetic Biology 2023Quote: 12 well plates were coated with HFN (1mL of HFN from Sigma added to 50mL PBS), by adding 700uL of PBS to the wells ...
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bioRxiv - Genomics 2023Quote: ... Cells were differentiated by addition of 10 ng/ml phorbol 12-myristate 13-acetate (Sigma, P1585) for 24 hours and treated with 100 mg/ml lipopolysaccharide (LPS ...
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bioRxiv - Cell Biology 2023Quote: ... All cell lines were tested every 6-12 months either by PCR (LookOut kit, Sigma/Merck) or Mycostrips (Invivogen ...
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bioRxiv - Immunology 2023Quote: ... Lysates migrated through 10 or 12% PAGE and transferred to PVDF membranes (Immobilon-P, EMD Millipore).
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bioRxiv - Cell Biology 2022Quote: Proteins were resolved on a 4-12% SDS-PAGE gel and transferred onto PVDF membranes (Millipore) using Criterion™ Blotter (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... plated at 500 cells/cm2 in gelatinized plates (12 wells) onto mitomycin-C (Sigma, Cat#M0503)-treated MEFs (10μg/mL mitomycin-C for 3 hours to inactivate MEFs) ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were resolved with 12% SDS-PAGE and transferred onto a PVDF membrane (Immobilon-P, Millipore). Flag-tagged H2B was detected with western blot using mouse anti-FLAG (F3165 ...
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bioRxiv - Cell Biology 2022Quote: Human neuroblastoma SH-SY5Y cells (ATCC CRL-2266) were maintained in DMEM/F-12 (Sigma-Aldrich) as described in Silveyra et al ...
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bioRxiv - Plant Biology 2022Quote: Transgenic lines were incubated in 12 well plates containing 25 μM of Brefeldin A (BFA, Sigma, www.sigmaaldrich.com ...
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bioRxiv - Neuroscience 2023Quote: ... and stained with Thioflavin-S 0.05% in 100% ethanol (ThioS, Sigma-Aldrich, Cat# 1326-12-1) for 5 min ...
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bioRxiv - Immunology 2022Quote: ... 6 × 106 MNC were transferred to permeable transwells (0.4-μm pore, 12-mm diameter; Millipore, (24)) ...
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bioRxiv - Neuroscience 2023Quote: 8-12 week-old male and female mice were trained to associate acetophenone (Sigma-Aldrich, 42163), lyral (IFF ...
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bioRxiv - Bioengineering 2023Quote: ... cells on nanovials in a 12-well plate were activated via 10 ng/mL PMA (Sigma) and 2.5 μM ionomycin (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were differentiated using 25 ng/mL phorbol-12-myristate-13-acetate (PMA, Sigma-Aldrich, USA) for 48 hours to obtain a macrophage-like phenotype ...
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bioRxiv - Genetics 2023Quote: ... Larva were incubated in Schneider’s insect medium (Thermo) with either 12 uM tunicamycin or DMSO (Sigma) control for 3 hours at 25°C ...
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bioRxiv - Developmental Biology 2023Quote: ... on Millicell™ 12 mm cell culture insert with 0.4 µm hydrophilic PTFE membrane (Sigma, #PICM01250). The cell culture insert was then submerged in RO media in a 24-well plate ...
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bioRxiv - Bioengineering 2024Quote: ... then cultured with added 10 ng/mL phorbol 12-myristate 13-acetate (PMA, Sigma catalog #524400) and 1 ug/mL ionomycin calcium salt (ionomycin ...
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bioRxiv - Developmental Biology 2024Quote: N2B27 defined basal medium contained 1:1 Neurobasal and DMEM/F-12 medium (Sigma-Aldrich D6421), Neurobasal medium (Life technologies 21103-049) ...
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bioRxiv - Immunology 2024Quote: ... and cells were stimulated with phorbol 12-myristate 13 acetate (PMA) (20 ng/ml, Sigma-Aldrich) and ionomycin (1μg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... Cuticles were then transitioned to Wash Buffer A (2x SSC, 10% Formamide (Sigma, 75-12-7), DEPC-Treated Water (Thermo ...
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bioRxiv - Molecular Biology 2024Quote: ... Four grams of 12-day-old seedlings were harvested and fixed with 1% formaldehyde (Sigma, F8775). Samples were ground in liquid nitrogen and homogenized in 40 ml of NEB1 buffer (10 mM Tris-HCl [pH 8.0] ...
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bioRxiv - Immunology 2024Quote: ... single-cell suspensions were stimulated with phorbol 12-myristate 13-acetate (PMA) (10 ng/ml) (Sigma) and ionomycin (250 ng/ml ...
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bioRxiv - Immunology 2024Quote: ... The extracts were separated on 12% SDS-PAGE gels and transferred to polyvinylidene difluoride membrane (Millipore). The membranes were probed with specific antibodies targeting RTF1 (Sigma) ...
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bioRxiv - Molecular Biology 2024Quote: ... with 10% FBS and differentiated into macrophages using phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich) as described previously 24 ...
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bioRxiv - Immunology 2024Quote: THP-1 cells were differentiated with 10 ng/mL phorbol 12-myristate 13-acetate (PMA) (Sigma) in 0.4 µm transparent PET membrane inserts (Falcon ...
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bioRxiv - Developmental Biology 2024Quote: ... both times for 8-12 minutes and in the presence of DNase I solution (Sigma Aldrich). FBS (Cytiva HyClone ...
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bioRxiv - Cancer Biology 2024Quote: THP-1 monocytes were differentiated to macrophages with phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich). THP-1 differentiation was optimized using ∼5×105 cells/mL in a 1 mL culture tube and treated with PMA to a final concentration of 25 ...
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bioRxiv - Neuroscience 2024Quote: ... The neurons were plated on 12-mm glass coverslips coated with poly-D-lysine (Sigma-Aldrich) in 24-well plates and cultured with Neurobasal medium (Gibco ...
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bioRxiv - Genetics 2024Quote: ... and incubated in cell culture media (DMEM/Nutrient mixture F-12 Ham powder, Millipore Sigma, D9785) at 37°C with 5% CO2 ...
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bioRxiv - Biochemistry 2024Quote: ... the blots were incubated with 10 mL HRP-linked mouse secondary antibody (Millipore Sigma 12-349) at a 1:4,000 dilution in TBST at room temperature for 1 hour ...
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bioRxiv - Bioengineering 2024Quote: ... adult mice age 6-12 weeks were injected i.p with 100 µl of tamoxifen (Sigma-Aldrich #) dissolved in corn oil or ChremophorEL:EtOH:PBS (ratio 1:1:2 ...
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bioRxiv - Cell Biology 2024Quote: ... then 12-15 embryos were transferred to tubes containing ice cold lysis buffer (CellLytic M [Sigma C2978] + protease inhibitor cocktail ...
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bioRxiv - Developmental Biology 2024Quote: ... 4× SDS sample buffer (0.225 M Tris-HCl pH6.8, 50% glycerol (Sigma-Aldrich, 12-1120-3), 5% SDS ...
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bioRxiv - Biophysics 2021Quote: ... and incubated on a shaker at 35°C for 30 min in the same solution with the addition of elastase type IV (0.6 mg/ml of 5.7 units per mg; Sigma, Chemical Co.), collagenase type 2 (0.8 mg/ml of 250 units per mg ...
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bioRxiv - Genetics 2020Quote: ... wild-type and mutant LIR-2s were N-terminally FLAG-tagged using the p3xFLAG-CMV-10 expression vector (SIGMA-Aldrich). For co-immunoprecipitation ...
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bioRxiv - Developmental Biology 2020Quote: ... mice (n= 4 samples per group) The samples were mechanically processed and digested in collagenase type 1 (3.68 mg/ml; Sigma-Aldrich) for 1 h and 20 min at 37°C in a shaker platform ...
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bioRxiv - Neuroscience 2021Quote: ... Acute inflammatory-type macrophages were induced by treating cells with 10 ng/mL lipopolysaccharide (from E. coli 055:B5, Sigma) for 3.5 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... was replaced by the wild-type CARD14 gene obtained from pFN21AE3344 with N-terminal 3xFLAG tag peptides (DYKDHDGDYKDHDIDYKDDDDK) (Sigma-Aldrich). To generate CARD14-expressing vector (pTRE3G-Pur-3FLAG-CARD14wt ...
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bioRxiv - Cell Biology 2022Quote: ... gastrocnemius muscles were enzymatically digested in a well containing 0.18% collagenase type I in Dulbecco modified eagles medium (DMEM) (Sigma-Aldrich) for ~2.5 hours ...
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bioRxiv - Immunology 2022Quote: ... GLS KO cells and their parental wild-type cells were cultured in RPMI cell culture medium (Sigma, St. Louis, MO) supplemented with 10% FBS (Thermo Fisher ...
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bioRxiv - Genomics 2020Quote: Twenty-four well plates were coated with: human or rat tail collagen type I (88 ng per well; Sigma-Aldrich), human collagen type III (88 ng per well ...
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bioRxiv - Genetics 2020Quote: HEK293 cells purchased from the American Type Culture Collection (CRL-1573) and were routinely passaged in Dulbecco’s Modified Eagle’s Medium (DMEM) (Millipore Sigma D6046) supplemented with 10% FBS and 100U/mL Penicillin-Streptomycin (referred to as “culture media” hereafter) ...
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bioRxiv - Cancer Biology 2021Quote: ... Chip channels were then coated with a mixture of ECM in Dulbecco’s phosphate-buffered saline (DPBS): 200 μg/mL human placenta collagen type IV (Sigma-Aldrich) and 30μg/mL fibronectin (Gibco ...