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Citations for Millipore Sigma :
151 - 200 of 10000+ citations for Nuclear pore complex protein Nup93 dye Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... The treated samples were washed again with TBST 5 times the second day and mounted with PBS+50% glycerol supplemented with Hoechst nuclear dye (Sigma, B2261, 1 μg/ml) and ready for imaging ...
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bioRxiv - Cell Biology 2024Quote: ... All experiments used dibenzocyclooctyne-PEG4-biotin (DBCO-biotin, Sigma) as the alkyne half of the cycloaddition ...
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bioRxiv - Biochemistry 2024Quote: ... All experiments used dibenzocyclooctyne-PEG4-biotin (DBCO-biotin, Sigma) as the alkyne half of the cycloaddition ...
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bioRxiv - Molecular Biology 2024Quote: ... DAPI nuclear staining (Sigma, D9564) was either included in the secondary antibody solution at a dilution of 1:5.000 or after this incubation DAPI was prepared in PBS 1X at a dilution of 1:5.000-1:10.000 and cells were incubated for 5 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... nuclear stained with DAPI (Sigma) and mounted in Vectashield® mounting media before imaging ...
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bioRxiv - Cell Biology 2024Quote: ... For nuclear staining DAPI (Sigma) was added to the normal staining or embedding medium supplemented with DAPI (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2024Quote: ... Nuclear Fast Red (Sigma-Aldrich) and hematoxylin were used as nuclear counterstains for alkaline phosphatase and peroxidase stainings ...
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bioRxiv - Developmental Biology 2024Quote: ... Hoechst nuclear stain (Sigma Aldrich) was added at 1:500 with secondary antibody for some experiments ...
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bioRxiv - Molecular Biology 2020Quote: ... Incubated RdRp complex protein was concentrated with a 100 kDa molecular weight cut-off Centrifugal Filter (Millipore Corporation) and then size-separated by a Superdex S200 10/300 GL column in 25 mM HEPES pH 7.4 ...
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bioRxiv - Cancer Biology 2020Quote: ... The pETDuet-1-His-BAP1-ULD+ASXL2-AB protein complex was expressed in Rosetta 2 (DE3) pLysS (Millipore). The bacteria bearing the desired plasmids were propagated with aeration at 37°C in 1L of 2YT to an A600 absorbance of approximately 0.6 ...
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bioRxiv - Genetics 2024Quote: ... complex was prepared 15 min before transfection by mixing gRNAs (1.5 µM) with Cas9 protein (0.45 µM) (Sigma, Catalog no ...
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bioRxiv - Physiology 2024Quote: ... or 10 mM succinate (complex II substrate) with 1µM rotenone (complex I inhibitor, Sigma-Aldrich R8875). After measuring oxygen consumption (State 3 ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were transferred to a polyvinylidene difluoride (PVDF) membrane (Immobilon, 0.2 μm pore, Millipore Corp., MA, USA) and incubated overnight at 4°C with primary antibody solution containing polyclonal rabbit anti-mouse ApoA-II antiserum (diluted 1:3000 ...
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bioRxiv - Developmental Biology 2022Quote: Separated proteins were wet transferred onto 0.45mm pore-sized PVDF membranes (Immobilon®-FL PVDF membrane, Millipore) for 2 hours at 250mA and 4°C using cold transfer buffer (1.5x NuPAGE transfer buffer (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were separated by SDS-PAGE and transferred onto 0.45 µm pore size Immobilon PVDF membrane (Millipore). PVDF membranes were blocked with TBS-T (50 mM Tris ...
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bioRxiv - Neuroscience 2024Quote: ... Protein from electrophoresed gels was transferred to PVDF membranes with 0.45 µM pore size (Millipore, cat# IPFL00010) by wet transfer in Towbin’s buffer [25 mM Tris ...
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bioRxiv - Developmental Biology 2024Quote: ... Separated proteins were electrotransferred to Immobilon-P polyvinylidene difluoride membranes with 0.45-μm pore size (#IPVH00010; Millipore). Membranes were blocked with 5% skim milk in Tris-buffered saline (TBS ...
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bioRxiv - Immunology 2023Quote: ... Total protein content was quantified using the Bradford dye-binding method (Sigma, #B6916). A total of 50 μg of cellular proteins were mixed with 5x loading buffer and boiled for 5 min ...
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bioRxiv - Cell Biology 2022Quote: Mitochondrial complex I inhibitor rotenone (Sigma), pan caspase inhibitor Z-VAD (OMe)-FMK (Abcam) ...
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bioRxiv - Microbiology 2020Quote: ... using Dimethyl-amine-borane complex (Sigma) and Formaldehyde (Thermo ...
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bioRxiv - Biochemistry 2021Quote: ... and mTORC1 complex (Sigma-Aldrich; SRP0364) at a 1:10 kinase:substrate mass ratio ...
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bioRxiv - Cell Biology 2020Quote: ... 5-hydroxytryptamine creatinine sulfate complex (Sigma) was added to NGM agar plates to a final concentration of 20 mM ...
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bioRxiv - Neuroscience 2024Quote: ... 1mM ribonucleoside vanadyl complex (Sigma-Aldrich), 10mM NaPO4 ...
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bioRxiv - Developmental Biology 2023Quote: ... Arp2/3 complex (Sigma-Aldrich MABT95), N-WASP (Thermo Fisher Scientific PA5-52198) ...
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bioRxiv - Biophysics 2024Quote: ... 2 mM vanadyl ribonucleoside complex (Sigma), and 1 mg/ml Escherichia coli tRNA (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... and bounded proteins were eluted with NETN buffer containing 2 mg/ml biotin (Sigma-Aldrich) for 1 h twice at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... Bait-purified prey proteins from plasma were then eluted with 5 mM biotin (Sigma-Aldrich) in ice-cold 1x PBS ...
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bioRxiv - Neuroscience 2020Quote: ... The primary antibodies (PV; 1:1000, Swant; Biotin-WFA; 1:100, Sigma-Aldrich) were incubated over night at 4 °C following rinsing tissues for 5 min three times in PBS-T (0.2 % Triton-X in PBS) ...
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Measuring adaptation dynamics to hydrogen peroxide in single human cells using fluorescent reportersbioRxiv - Cell Biology 2020Quote: ... MCF7 growing cells are routinely screened for the presence of mycoplasma using DNA-staining with the nuclear dye Hoechst 33342 (1:10000 dilution) (Sigma-Aldrich, L’Isle d’Abeau Chesnes, France) to avoid collecting data from unknowingly contaminated cell cultures.
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bioRxiv - Genetics 2021Quote: Three-fold diluted nuclear extracts were pre-cleared with 50 μL Protein-G agarose beads (Millipore, 16-266) for 1 hour at 4°C and then incubated overnight on a rotor with 3 μL anti-FLAG antibody (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... To validate successful separation of cell fractions protein levels of PARP1 (nuclear control: Sigma-Aldrich, Cat.-No. HPA045168) and GAPDH (cytoplasmic control ...
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bioRxiv - Cancer Biology 2023Quote: ... The nuclear protein lysate then was pre-cleared by adding 2 μL of poly D[IC] (Sigma-Aldrich) 25 µg/µL and 20 μL of High Capacity Strepavidin agarose beads (Thermofisher #20357) ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... immunoblotting with antibody against the nuclear marker Lamin-A/C was performed (Sigma-Aldrich SAB4200236,1:1000).
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bioRxiv - Molecular Biology 2023Quote: ... secondary antibody was incubated at room temperature for 1h followed by DAPI nuclear staining (Sigma, Germany). Immunostainings were examined with an immunofluorescence microscope (Leica) ...
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bioRxiv - Microbiology 2022Quote: ... of 3.5 mg/mL mixtures of purified STFK or STFK1628x proteins in complex with excess Fab fragments of nAbs were incubated in 0.01% (v/v) Digitonin (Sigma) and then loaded onto glow-discharged (60 s at 20 mA ...
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bioRxiv - Microbiology 2020Quote: ... the mice were injected with 700 μg of His-tagged TGT complex protein in incomplete Freund’s adjuvant (Sigma-Aldrich). One week after the 6-week injection ...
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bioRxiv - Biochemistry 2023Quote: ... Protein complex-containing fractions were collected and concentrated using 10 kDa molecular weight cut-off centrifugal filters (Merck Millipore). The concentrated protein was split into two samples ...
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bioRxiv - Molecular Biology 2023Quote: ... the protein complex was eluted with 500 μL elution buffer (0.4 mg/ml 3X Flag peptide (Millipore Sigma F4799) in wash buffer ...
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bioRxiv - Biochemistry 2020Quote: ... NHS-biotin (Sigma) was dissolved in DMSO and added to the concentrated protein at a molar ratio of 10:1 such that the final DMSO concentration did not exceed 5% ...
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bioRxiv - Neuroscience 2020Quote: ... Biotin (40μM, Sigma) was added at t=0 ...
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bioRxiv - Genetics 2022Quote: ... biotin (Sigma, B4501) was added into the medium to the final concentration of 500 μM ...
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bioRxiv - Cell Biology 2021Quote: ... Biotin-phenol (Sigma).
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Stromal transdifferentiation drives lymph node lipomatosis and induces extensive vascular remodelingbioRxiv - Pathology 2022Quote: ... 0.0033% Biotin (Sigma), in the case of biotin-streptavidin amplification ...
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bioRxiv - Molecular Biology 2024Quote: ... Biotin (Sigma-Aldrich; #B4501 ...
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bioRxiv - Molecular Biology 2023Quote: ... Biotin (Sigma, B4639) was dissolved in 60 mM NaOH and stored at -20 °C in the dark ...
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bioRxiv - Immunology 2023Quote: ... biotin (Sigma-Aldrich) was added to a final concentration of 500 μM ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.001% Biotin (Sigma), 10000 U/mL penicillin/streptomycin solution and kept at 26 °C [97] ...
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bioRxiv - Neuroscience 2024Quote: ... biotin (Sigma B4639) was added to 50uM in the media ...
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bioRxiv - Molecular Biology 2024Quote: ... Biotin (B7653, Sigma), HMCES (NPB2-14410 ...
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bioRxiv - Genomics 2020Quote: Approximately 5 × 106 cells from the overnight incubation with the primary/secondary antibody-antibody complex was stringently washed with NET-2 buffer then denatured in a DL-Dithiothreitol (DTT) (Sigma-Aldrich D9779), NuPAGE™ LDS Sample Buffer (4X ...