Labshake search
Citations for Millipore Sigma :
101 - 150 of 10000+ citations for Nuclear pore complex protein Nup93 dye Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2024Quote: ... Recombinant proteins EZH2 (SRP0379) and PRMT5:MEP50 complex (SRP0145-25470 were purchased from Sigma-Aldrich United states ...
-
bioRxiv - Neuroscience 2023Quote: ... mouse anti-NeuN biotin conjugated antibody (1:2,000; MAB377B, Millipore), rabbit anti-parvalbumin (1:5,000 ...
-
bioRxiv - Microbiology 2021Quote: ... proteins were transferred to Immobilon-PSQ PVDF membrane (0.2 μm pore size, Millipore Sigma) by electrophoresis at 100 V for 30 minutes in Tris-Glycine/Methanol buffer ...
-
bioRxiv - Cancer Biology 2021Quote: ... The proteins were transferred to PVDF (polyvinylidene difluoride) membranes (0.2 μm pore size, Millipore) by immersion transfer (Bio-Rad) ...
-
bioRxiv - Biochemistry 2021Quote: ... The proteins were ultrafiltrated using 10 K pore size Amicon Ultra filter units (Millipore) and step-wise dialyzed against 5 mM Pipes-Na pH 7.0 ...
-
bioRxiv - Cell Biology 2024Quote: ... the proteins were transferred onto 0.45 nm pore polyvinylidene fluoride (PVDF) membrane (Millipore, USA) using an eBlot™ L1 wet transfer (GenScript Biotech ...
-
bioRxiv - Genomics 2022Quote: ... Proteins were transferred to a PVDF membrane (Millipore; Immobilon-FL, 0.45 mm pore size) in transfer buffer (25 mM tris ...
-
bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred to PVDF membranes (pore size 0.45 µm, Immobilon-P, Merck Millipore) using Trans-Blot Turbo Blotting System (BioRad ...
-
bioRxiv - Systems Biology 2024Quote: ... Proteins were transferred to Immobilon PVDF membranes (pore size 0.45 μM, Merck Millipore IPVH00010) for 1 h at 100 V ...
-
bioRxiv - Bioengineering 2022Quote: Biotinylated proteins were obtained using Biotin N-hydroxysuccinimide ester reagent (Sigma Aldrich) according to the manufacturer’s protocol.
-
bioRxiv - Cell Biology 2021Quote: ... and the protein concentration was estimated using the Bio-Rad Protein Assay Dye Reagent (Sigma). 10 µl of liver lysate was transferred to 96-well plate and ALT was measured using a microplate-based ALT activity assay kit (Pointe Scientific ...
-
bioRxiv - Neuroscience 2020Quote: ... The secondary antibody was washed off three times with PBS-tween and the cells were incubated in the nuclear dye DAPI (1:1000 in PBS, Sigma, D9452) for 10 min ...
-
bioRxiv - Molecular Biology 2023Quote: Proximity ligation assay using antibodies to RADX and an anti-biotin antibody to recognize EdU after conjugation to a biotin-azide were completed according to the manufacturer’s protocol (Sigma) and images obtained and quantified using a Molecular Devices ImageXpress instrument.
-
bioRxiv - Neuroscience 2021Quote: ... corticosterone:HBC complex (Sigma-Aldrich) was dissolved in saline and administered at 2mg/Kg IP ...
-
bioRxiv - Cancer Biology 2024Quote: ... 5 mg of nuclear protein was concentrated in 500ul using a Microcon centrifugal filter (Millipore) and then applied to a Superose 6 size exclusion column (10/300 GL GE Healthcare ...
-
bioRxiv - Biochemistry 2021Quote: ... the N protein was labelled with ATTO 488 dye (Sigma Aldrich) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... Bradford dye for protein estimation was purchased from Sigma-Aldrich (USA). All the primers were synthesized by GCC Biotech (India ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were incubated with a mouse anti-DENV complex monoclonal antibody (MAB8705, Merck Millipore), washed three times with PBS ...
-
bioRxiv - Immunology 2023Quote: ... or rabbit anti-ARPC3 (anti-Arp2/3 Complex Antibody Clone 13c9 Millipore #MILL-MABT95). HRP-conjugated goat anti-rabbit IgG (Cell Signaling #7074S ...
-
bioRxiv - Biochemistry 2020Quote: ... proteins were transferred to a 0.45 μm pore size polyvinylidene fluoride transfer membrane (Merck Millipore), and nonspecific binding was blocked in Tris-buffered saline/0.1% Tween 20 with 5% skim milk powder ...
-
bioRxiv - Systems Biology 2021Quote: ... proteins were transferred onto a PVDF membrane (pore size 0.45 mm, Immobilon-P, Millipore, www.millipore.com) (66 ...
-
bioRxiv - Biochemistry 2021Quote: ... proteins were transferred onto methanol-preactivated Immobilon-P PVDF membranes (pore size 0.45 μm; Millipore) using a semi-dry transfer cell or a wet transfer tank (Bio-Rad) ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred from gels to 42-μm-pore Millipore PVDF membranes (Sigma-Aldrich, MO) for 1 h at 30 V ...
-
bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to 0.45 µm pore size polyvinylidene difluoride membranes (PVDF) (Millipore, catalog # IPVH00010), incubated with rabbit anti-LC3B antibody (1:1000 ...
-
bioRxiv - Neuroscience 2024Quote: ... Protein samples were transferred onto a 0.45 μm pore polyvinylidene difluoride (PVDF) membrane (IPVH00010, Millipore), previously activated in cold 100% methanol ...
-
bioRxiv - Bioengineering 2024Quote: ... Proteins were then transferred to 0.45 µm pore size Immobilon-FL PVDF membranes (Millipore-Sigma) using a wet tank system at 10V for 16h at 4°C that included a cooling pack and a magnetic stir plate under constant agitation to keep the system from heating up ...
-
bioRxiv - Bioengineering 2024Quote: ... Proteins were then transferred to 0.45 µm pore size Immobilon-FL PVDF membranes (Millipore-Sigma) using a wet tank system at 10V for 16h at 4°C that included a cooling pack and a magnetic stir plate under constant agitation to keep the system from heating up ...
-
bioRxiv - Cell Biology 2023Quote: ... nuclear/cytosolic fractions were obtained using a nuclear extraction kit (Millipore, 2900). All samples were boiled for 5 mins prior to analysis.
-
bioRxiv - Molecular Biology 2022Quote: ... Nuclear counterstaining was done with Nuclear Fast Red (Sigma Aldrich, Darmstadt, Germany) for 1 min and then washed in 37°C tap water for 10 min before mounting.
-
bioRxiv - Cancer Biology 2024Quote: ... antibody obtained from Cell Signaling Technology and rabbit secondary antibody conjugated to biotin obtained from Sigma Aldrich. The staining intensity was assessed on a scale from 0 (no staining ...
-
bioRxiv - Cancer Biology 2023Quote: ... samples were incubated with biotin-conjugated hyaluronic acid binding protein (HABP) (Sigma 385911), followed by streptavidin conjugated to AlexaFluor-488 (ThermoFisher S11223) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Biotin switch-treated protein reduced with 2.5 μL of 0.2 M dithiothreitol (Sigma) for 1 hr at 57°C and the samples cooled before adding 2.5 μL of 0.5M iodoacetamide and incubated in the dark for 45 minutes ...
-
bioRxiv - Developmental Biology 2020Quote: ... Nuclear fast red (Sigma) was used to stain 5 mM sections in Supplementary Fig ...
-
bioRxiv - Neuroscience 2023Quote: ... Nuclear fast red (Sigma) staining was used as nuclear counterstaining.
-
bioRxiv - Molecular Biology 2020Quote: Liver nuclear and cytosolic proteins separated by SDS-PAGE were transferred to polyvinylidene difluoride membrane (Millipore). The blots were blocked in a blocking solution (1%[w/v] skim milk in TBS [50 mM Tris-HCl ...
-
bioRxiv - Plant Biology 2023Quote: Nuclear proteins were extracted from frozen poplar stem using the CellLytic™ PN kit (Sigma, USA) according to the manufacturer’s instruction ...
-
bioRxiv - Microbiology 2021Quote: ... Filipin complex (Sigma-Aldrich, F9765), Nigericin sodium salt (Cayman Chemical ...
-
bioRxiv - Neuroscience 2024Quote: ... or CF dye-conjugated IgG secondary antibodies (1:250, Sigma) in blocking solution and mounted in Aqua-mount (Southern Biotech ...
-
bioRxiv - Biochemistry 2020Quote: ... Protein complex was incubated overnight on ice and then concentrated onto 30 kDa MWCO Amicon concentrator (Millipore) until protein concentration reached 11 mg/ml ...
-
bioRxiv - Molecular Biology 2022Quote: ... The ORF1p-FLAG protein complex bound was eluted using 200 μg/mL of 3xFLAG peptide (Sigma-Aldrich) in the Lysis150 buffer containing 0.2 mM PMSF and 1x cOmplete EDTA-free protease inhibitor cocktail by incubation at 4°C for 1 hour with gentle rotation ...
-
bioRxiv - Genetics 2021Quote: ... Proteins were separated using SDS-PAGE and transferred to 0.45-μm pore size PVDF membranes (Millipore). Membranes were blocked in 5% BSA for 1 h before overnight incubation with primary antibodies diluted in Tris-buffered saline with 0.1% tween (TBST) ...
-
bioRxiv - Genomics 2021Quote: Protein samples were transferred onto a immobilon-PSQ 0.2 μM pore size PVDF membrane (Merk Millipore). The PVDF membrane was blocked by 3% BSA in 0.05% PBST (0.05% Tween-20 in 1 x PBS ...
-
bioRxiv - Cancer Biology 2022Quote: ... biotin (Sigma) was added to a final concentration of 50μM to transduced cells ...
-
bioRxiv - Neuroscience 2020Quote: ... Biotin (Sigma) was added to the media on DIV13 at a final concentration of 100μM ...
-
bioRxiv - Microbiology 2024Quote: ... purified RSP protein was mixed with Biotin-NHS (Biotinamidocaproate N-hydroxysuccinimide ester, Sigma Aldrich) and three rounds of MACS (with RSP concentrations of 100 nM (the first one ...
-
bioRxiv - Neuroscience 2022Quote: ... The primary antibodies used were: goat anti-Biotin (Sigma, F6762, 1:80), rabbit anticalcium sensing receptor (abeam ...
-
bioRxiv - Molecular Biology 2020Quote: Complex III activity was assayed using a mitochondrial complex III activity assay kit (Sigma Aldrich) and complex IV activity was determined using a complex IV human enzyme activity microplate assay kit (Abcam) ...
-
bioRxiv - Molecular Biology 2022Quote: ... Nuclear counterstaining was performed with Nuclear Fast Red (Sigma-Aldrich, Saint Louis, MO, USA).
-
bioRxiv - Molecular Biology 2020Quote: Immunoprecipitations were performed on 500 μg of nuclear protein extracts using anti-flag M2 gel (Sigma-Aldrich) or by using Diagenode A/G protein-coupled magnetic beads to target endogenous proteins ...
-
bioRxiv - Cell Biology 2022Quote: ... The treated samples were washed again with TBST 5 times the second day and mounted with PBS+50% glycerol supplemented with H33342 nuclear dye (Sigma, B2261, 1 μg/ml) and ready for imaging ...