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Citations for Millipore Sigma :
51 - 100 of 10000+ citations for Nuclear pore complex protein Nup93 dye Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Vibratome sections (80 μm thick) were obtained and counterstained with the fluorescent nuclear dye DAPI (Sigma-Aldrich). Three sections per animal were imaged using a Leica K5 camera into Leica DMi8 microscope ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were counterstained with the nuclear dye 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI, Sigma-Aldrich, Germany) and actin filaments were labeled with ATTO 647-phalloidin conjugate (Hypermol EK ...
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bioRxiv - Cancer Biology 2023Quote: Treated living cells were stained with the nuclear dye 4’6-diamidino-2-phenylindole (DAPI; Sigma, Cat # D9542) at a final concentration of 5 µg/mL for 20 minutes at 37 °C prior to live cell imaging by epifluorescence (Leica DMI6000B) ...
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bioRxiv - Systems Biology 2023Quote: ... the stretched cells were stained with BioTracker 650 red nuclear dye (Sigma-Aldrich Chemie N.V., Zwijndrecht, Netherlands), followed by stimulation with recombinant mouse IFNα or IFNβ1 (BioLegend Uk Limited ...
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bioRxiv - Cell Biology 2022Quote: ... The protein complex was concentrated using centrifuge filters (Amicon Ultracel-30K, Millipore) and subjected to SEC using a Superdex 200 column (HiLoad 16/600 Superdex 200 pg ...
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bioRxiv - Biochemistry 2024Quote: ... The dialysed protein complex was then bound to Ni2+-NTA resin (Sigma) and eluted over a 0.01 – 0.5 M imidazole gradient ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were then analyzed by western blotting using a primary anti-biotin antibody (1:5,000 dilution; Sigma-Aldrich) and an anti-mouse secondary antibody coupled to peroxidase (1:10,000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... The beta subunit of complex V and proteins bound to it were immunoprecipitated using an antibody to the beta subunit of ATP synthase (Millipore, MAB3494) in accordance with the Pierce™ Co-Immunoprecipitation Kit (Cat.No:26149) ...
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bioRxiv - Microbiology 2023Quote: ... lysates corresponding to 1 OD600 of cells were analyzed by SDS-PAGE and immunoblotted with either anti-c-Myc (9E10, Covance Research) for Myc-tagged proteins or anti-peroxidase soluble complex antibody (Sigma, P2416) for TAP-tagged proteins ...
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bioRxiv - Genetics 2022Quote: ... TAP-tagged Rrp45 protein was detected with peroxidase anti-peroxidase (PAP) soluble complex antibody produced in rabbit (1:5000, Sigma-Aldrich). The 3-phosphoglycerate kinase (Pgk1 ...
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bioRxiv - Genetics 2020Quote: ... Nuclear and cytoplasmic protein extracts were prepared using NXTRACT kit (Sigma-Aldrich) according manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: Biotin-anti-6xHis antibodies (SIGMA, MA1-21315-BTIN) were flowed in through a streptavidin-coated flow channel ...
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bioRxiv - Neuroscience 2020Quote: ... tissue was incubated at 4°C in blocking solution with Hoechst 33342 nuclear dye (Sigma, H21492, 1:2000) for 24 hours ...
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bioRxiv - Neuroscience 2024Quote: Pressured-fixed MRAs were stained with the nuclear dye Hoechst 33342 (0.01 mg/ml; Sigma-Aldrich, Cat#: 14533) for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... A commercial mouse anti-DENV complex monoclonal antibody (MAB8705; Merck Millipore) diluted 1:200 in phosphate-buffered saline (PBS ...
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bioRxiv - Physiology 2023Quote: Glycerol stocks of pLKO.1-puro lentiviral plasmid vectors containing shRNA targeting human Nup93 (NM_014669) and an empty vector insert (shEmpty) were purchased from Sigma-Aldrich. Lentivirus expressing shNup93 or shEmpty were generated by PEI-mediated co-transfection of pLKO.1-puro ...
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bioRxiv - Molecular Biology 2020Quote: ... TAP-tagged proteins were detected with peroxidase–anti-peroxidase– soluble complex (P1291, Sigma). α-tubulin was detected with antibody T5168 (Sigma).
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bioRxiv - Microbiology 2020Quote: ... The protein-DNA complex was treated with 1 U/μl DNase I (Novagen) to digest non-specific DNA ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was filtered with a 0.22 μm pore size filter (Millipore) prior to size-exclusion chromatography (SEC ...
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bioRxiv - Neuroscience 2023Quote: ... The dialyzed protein solution was filtered (0.45 μm pore size filter, Millipore) and protein concentration was determined by absorbance measurements at 280 nm using extinction coefficient ε = 37752 M-1cm-1 ...
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bioRxiv - Neuroscience 2023Quote: ... The dialyzed protein solution was filtered (0.45 μm pore size filter, Millipore) and protein concentration was determined by absorbance measurements at 277 nm using extinction coefficient ε = 5840 M-1cm-1 ...
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bioRxiv - Neuroscience 2020Quote: ... revelation was performed using an avidin/biotin complex coupled with peroxidase (ABC kit, Universal Vectastain Kit PK6200) and diaminobenzidine (Sigma Aldrich).
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An innovative hematopoietic stem cell gene therapy approach benefits CLN1 disease in the mouse modelbioRxiv - Neuroscience 2022Quote: ... sections were washed trice with PBS and incubated for 1h at RT with Avidin-Biotin complex (Vector) and then with DAB/H2O2 mix (Sigma-Aldrich).
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bioRxiv - Biochemistry 2020Quote: ... pH 7.0 and SYPRO Orange protein stain dye (Sigma). Melting curves were recorded with a temperature range of 20 to 95 °C ...
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bioRxiv - Cell Biology 2020Quote: ... The total nuclear protein was lysed with CHAPS buffer containing protease inhibitor (Sigma) and phosphatase inhibitor (Roche Applied Science ...
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bioRxiv - Neuroscience 2020Quote: ... Bound DNA and proteins were eluted with 16 mM biotin (Sigma) in water (at pH 7.0 ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein complex was eluted in Strep Buffer supplemented with 2.5 mM desthiobiotin (Sigma) at 1 mL/min ...
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bioRxiv - Plant Biology 2024Quote: ... The protein complex was eluted using 3 mg/ml maltose (Sigma-Aldrich®; USA). Eluted proteins were denatured and separated on SDS-PAGE gel and analyzed by immunoblotting with anti-GST antibody (1:4000 ...
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bioRxiv - Plant Biology 2024Quote: ... Protein complex was eluted using 2.8 mg/ml reduced glutathione (Sigma-Aldrich®; USA). Alternatively ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were then transferred to 0.45 µm pore PVDF (catalog number IPVH00010, Millipore) at 25 to 35 V for 1 hour and incubated with appropriate antibodies overnight at 4 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins were transferred onto nitrocellulose membranes (0.45 µm pore size; Sigma, cat. # GE10600012) for 2.5 h at 400 mA constant ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred onto PVDF membranes (Immobilon-FL, 0.45 μm pore size, Millipore) following standard procedures ...
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bioRxiv - Microbiology 2024Quote: ... The protein was concentrated using Amicon centrifugal units with 10kDa pore size (Millipore), the final protein concentration was evaluated by Bradford assay and protein purity was assessed by SDS-PAGE ...
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bioRxiv - Neuroscience 2023Quote: ... and immunoreactivity was visualized by the avidin-biotin complex method and sections were developed in diaminobenzidine (DAB, Sigma Aldrich, St. Louis, USA).
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bioRxiv - Molecular Biology 2021Quote: ... For the detection of biotin-labelled PARylated proteins the same assay was conducted in presence of biotin-NAD+ (Sigma Aldrich) followed by SDS-PAGE and western blot detection with anti-streptavidin HRP antibody (Molecular Probes).
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies were conjugated to IR-Dyes (Sigma) and detected using Odyssey CL or CLx infrared scanners (LiCor).
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bioRxiv - Immunology 2023Quote: ... Nuclear staining was performed with Hoechst 33342 nuclear stain (Sigma). The nasal septum was embedded using a glycerol-based mounting media and hardened under applied pressure ...
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bioRxiv - Immunology 2023Quote: ... Nuclear staining was performed with Hoechst 33342 nuclear stain (Sigma).
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bioRxiv - Biochemistry 2021Quote: ... the proteins were incubated with SYPRO orange dye (Sigma-Aldrich), which binds specifically to hydrophobic amino acids leading to an increased fluorescence at 610 nm when excited with a wavelength of 490 nm ...
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bioRxiv - Biochemistry 2020Quote: ... the proteins were incubated with SYPRO orange dye (Sigma-Aldrich), which binds specifically to hydrophobic amino acids leading to an increased fluorescence at 610 nm when excited with a wavelength of 490 nm ...
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bioRxiv - Cell Biology 2020Quote: ... and nucleoproteins were extracted using a nuclear protein extraction kit (Merck Millipore, Darmstadt, Germany). Nucleoproteins were separated by electrophoresis on 6/12% sodium dodecyl sulfate (SDS ...
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bioRxiv - Cell Biology 2021Quote: Nuclear extracts were pre-cleared by incubation with 40 μl Protein A dynabeads (Sigma) for one hour at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Total or nuclear protein was extracted using a nuclei isolation kit (NUC201, Sigma-Aldrich). Protein samples were electrophoresed on a 10% sodium dodecyl sulfate-polyacrylamide gel and transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Genetics 2023Quote: ... Immunoprecipitation of nicks were carried out according to standard ChIP protocols using Protein G-coupled Dynabeads coated with monoclonal anti-biotin antibodies (Sigma-Aldrich) antibody ...
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bioRxiv - Biochemistry 2021Quote: The following antibodies were used: Peroxidase anti-peroxidase complex (PAP) (Sigma, cat# 1291) to detect TAP ...
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bioRxiv - Cell Biology 2024Quote: ... The mRNA-ribosome complex was precipitated using a polyclonal HA-antibody (Sigma #H6908) and Dynabeads Protein G (Life Technologies #10004D ...
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bioRxiv - Plant Biology 2023Quote: ... The complex was then incubated with 2 μg of anti-HA antibody (Sigma). The precipitated DNA was recovered and ChIP qPCR was performed using EpiQuik™ Chromatin Immunoprecipitation (ChIP ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated in biotinylated anti-rabbit secondary antibody 1:500 (Vectors Laboratories, #BA-1000) followed by Avidin/Biotin complex amplification (Vectastain Elite ABC kit; Vectors Laboratories, #PK-6100) and DAB (Sigma Life Science, #5905) mixed with 3% hydrogen peroxide for signal amplification ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein A-Sepharose (P3391) and PRMT5:MEP50 active complex (SRP0145) were purchased from Sigma Aldrich. Protein co-immunoprecipitation was performed using ANTI-FLAG® M2 Affinity Gel (A2220 ...
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bioRxiv - Cancer Biology 2024Quote: ... Recombinant proteins (PRMT5:MEP50 complex (SRP0145-25470) and EZH2 (SRP0379) were purchased from Sigma-Aldrich USA ...