Labshake search
Citations for Millipore Sigma :
1851 - 1900 of 5383 citations for Recombinant Human Insulin Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2022Quote: ... AMPA-receptor-mediated excitatory postsynaptic currents (EPSCs) were recorded in presence of picrotoxin (100 μM, Sigma Aldrich) to block GABAARs ...
-
bioRxiv - Bioengineering 2023Quote: ... The stimulation of ACh to muscarinic receptors was blocked by addition of 10 nM Atropine (Sigma Aldrich). In the presence of Atropine ...
-
bioRxiv - Neuroscience 2024Quote: ... 2) GluR2 to visualize postsynaptic receptors (mouse anti-GluR2 at 1:2000, Millipore, catalog # MAB397, RRID: AB_2113875) and 3 ...
-
bioRxiv - Neuroscience 2024Quote: ... Aladdin) or β receptor agonist isoprenaline (2 mg/mL, 4 mg/mL, n = 7, I5627, Sigma-Aldrich) or β receptor antagonist propranolol (2·5 mg/mL ...
-
bioRxiv - Immunology 2023Quote: ... Anti-human IgG HRP antibody (Anti-Human IgG-Peroxidase from Sigma) was diluted 1:30,000 in blocking buffer ...
-
bioRxiv - Cell Biology 2020Quote: ... N2 (2x stock in Advanced DMEM/F-12 (Fisher) with 25 μg/mL insulin (Sigma), 100 μg/mL apo-Transferrin (Sigma) ...
-
bioRxiv - Systems Biology 2022Quote: ... T-47D media was additionally supplemented with 0.2 units/mL Bovine Insulin (Sigma Aldrich, IO516). Cells were regularly passaged and tested for presence of mycoplasma contamination (Mycoplasma PCR Detection Kit ...
-
bioRxiv - Neuroscience 2020Quote: ... Blood MeS markers were then quantified using an ELISA kit (insulin: EZRMI-13K, Millipore; glucose) and colorimetric assays (triglyceride ...
-
bioRxiv - Molecular Biology 2021Quote: ... The insulin concentration in each perifusion fraction and islet extract was measured by radioimmunoassay (Millipore).
-
bioRxiv - Cell Biology 2019Quote: ... 1 μg/mL insulin and 0.5 mM of 3-isobutyl-1-methylxanthine (IBMX) (Sigma, #I5879) complemented with budesonide (Selleck Chemicals ...
-
bioRxiv - Biochemistry 2021Quote: ... primary hepatocytes were first pre-incubated in M199 supplemented with 100nM insulin (Sigma, Cat#10516) for lipogenesis activation ...
-
bioRxiv - Cell Biology 2021Quote: ... 5 μg/ml insulin (#I9278) and 2 × 10−11 M liothyronine (all Sigma-Aldrich, #T6397). HEK293T cells (a gift from Noor Gammoh ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Plasma insulin and plasma c-peptide were measured using radioimmunoassay (EMD Sigma, Cat #: RI-13K) and bead immunoassay (Luminex ...
-
bioRxiv - Physiology 2022Quote: ... Serum insulin was determined as previously described according to the manufacture’s instruction (Millipore #EZMI-13K)(16).
-
Adipocyte vesicles: ‘all-in-one’ packages that stimulate tumor mitochondrial metabolism and dynamicsbioRxiv - Cancer Biology 2019Quote: ... bovine serum albumin (BSA) and insulin were obtained from Sigma Aldrich (Saint Louis, MO, USA). Lalistat 2 was obtained from Tocris Cookson (Bristol ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Next day we replace the medium with 100 μl DMEM ± 1 μM insulin (Sigma I0516) without serum or glucose and incubate for 1 hour and then fixed with 3.7% formaldehyde for 20 minutes ...
-
bioRxiv - Physiology 2019Quote: ... Serum insulin and testosterone levels were measured using ELISA kit purchased from Millipore (#EZRMI-13K) and Crystal Chem (#80552) ...
-
bioRxiv - Biochemistry 2019Quote: ... Lyophilized nisin powder (2.5% nisin content) and insulin chain B were obtained from Sigma-Aldrich. The leader peptide of NisA was synthesized and obtained from JPT peptide technologies ...
-
bioRxiv - Cell Biology 2020Quote: ... 100 ng ml-1 cholera toxin and 10 µg/ml insulin (all from Sigma-Aldrich). Where indicated ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... were added directly to 1 mL of insulin (11061-Sigma-Aldrich, St. Louis, MO, USA) at the concentration of 10 mg/mL ...
-
bioRxiv - Cancer Biology 2021Quote: ... T-47D media was additionally supplemented with 0.2 units/mL Bovine Insulin (Sigma Aldrich, IO516). Cells were regularly passaged and tested for presence of mycoplasma contamination (Mycoplasma PCR Detection Kit ...
-
bioRxiv - Neuroscience 2023Quote: ... The plasma insulin concentrations were measured using an RIA assay (Millipore, St. Charles, MI, USA). Leptin concentrations were determined by ELISA (Millipore ...
-
bioRxiv - Physiology 2022Quote: ... for glucose tolerance test or injected with bovine insulin (0.75 U/kg bodyweight; Sigma, USA) for insulin tolerance test ...
-
bioRxiv - Genomics 2023Quote: ... supplemented with 10% fetal bovine serum (Gemini) and 10µg/ml insulin from bovine pancreas (Sigma, made as a 1000x solution in 1% aqueous glacial acetic acid).
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were resuspended in Dulbecco’s Modified Eagle Medium supplemented with insulin (10 µg/mL, Sigma), 10% fetal bovine serum ...
-
bioRxiv - Systems Biology 2023Quote: ... dishes in RPMI-1640 defined medium supplemented with 250 ng/ml insulin (Sigma-Aldrich, I6634), 0.5 μg/ml hydrocortisone (Sigma-Aldrich ...
-
p110α-dependent hepatocyte signaling is critical for liver gene expression and its rewiring in MASLDbioRxiv - Cell Biology 2024Quote: Plasma FGF21 and insulin were assayed using the rat/mouse FGF21 ELISA kit (EMD Millipore) and the ultrasensitive mouse insulin ELISA kit (Crystal Chem) ...
-
bioRxiv - Plant Biology 2019Quote: ... Immuno-blotting was performed using anti-ubiquitin antibody and anti-His (Sigma, USA).
-
bioRxiv - Biochemistry 2020Quote: 25 μl of the monoclonal α-His antibody (Catalog-ID H1029, Sigma-Aldrich) were mixed with 100 μl buffer comprising 50 mM HEPES-NaOH ...
-
bioRxiv - Cell Biology 2021Quote: Ezrin constructs in pET-28a(+) (N-terminal hepta-His tag, Novagen, Madison, WI): hEzrin wildtype (His-Ezrin/WT ...
-
bioRxiv - Biochemistry 2020Quote: ... The Gag-His6 protein was loaded onto a HIS-select affinity column (Sigma), washed (20 mM Tris-HCl ...
-
bioRxiv - Cell Biology 2021Quote: ... SC –leu-trp plates and SC –leu-trp-his + 10mM 3-aminotriazole (Sigma) plates ...
-
bioRxiv - Neuroscience 2020Quote: ... and HIS dihydrochloride (Sigma-Aldrich; 10 μg/10 μl; 9 mM; 90 nmol), as well as a combination of 2 substances (ALA 90 μg with BAM8-22 1 μg in 10 μl ...
-
The formation of a fuzzy complex in the negative arm regulates the robustness of the circadian clockbioRxiv - Molecular Biology 2022Quote: ... and Western blotting with primary anti-His at 1:1000 (Sigma Aldrich, SAB1305538), secondary anti-Mouse at 1:10000 (ThermoFisher ...
-
bioRxiv - Molecular Biology 2022Quote: ... which coeluted with His-TrxA or monoclonal anti-polyHistidine-Peroxidase antibody (Sigma-Aldrich) for the detection of recombinant His-tagged TrxA ...
-
bioRxiv - Bioengineering 2022Quote: ... Cells were washed with a washing solution of 4% HI-FBS (#F4135; Sigma) in PBS and permeabilized with 0.3% Triton X-100 (#T8787 ...
-
bioRxiv - Synthetic Biology 2021Quote: ... and Guanidine Hydrochloride (Serva, 24205.02) via Hi-Trap Desalting column (Sigma Aldrich GE17140801) for further experiments ...
-
bioRxiv - Biochemistry 2021Quote: ... The supernatant was incubated with 15 ml HIS-Select Nickel Affinity Gel (Sigma) for 1 h at 4°C ...
-
bioRxiv - Molecular Biology 2021Quote: ... Fractions containing His-Pap1 were pooled and concentrated using 30,000 MWCO concentrators (Millipore).
-
bioRxiv - Biochemistry 2022Quote: ... cleared cell lysates were captured by Ni-NTA His-bind resin (Merk Millipore) equilibrated in high salt buffer (50 mM Tris pH8 ...
-
bioRxiv - Microbiology 2022Quote: ... purified from bacterial lysates on a nickel affinity resin (His-Select, Sigma-Aldrich) as described (12) ...
-
bioRxiv - Biochemistry 2022Quote: ... The supernatant was then incubated with HIS-Select nickel affinity gel (Sigma-Aldrich) for 1 h ...
-
bioRxiv - Cell Biology 2023Quote: ... Affinity chromatography (HIS-Select™ Nickel Affinity Gel; #P-6611; Sigma Aldrich Inc.) was then used to purify His6-tagged caltubin from the cell lysate and the protein was eluted with 125 mM imidazole (in lysis buffer) ...
-
bioRxiv - Molecular Biology 2023Quote: ... the protein was re-applied to His-Select Cobalt affinity gel (Sigma-Aldrich). The flow through fractions were pooled and concentrated (Vivaspin Turbo 3,000 MWCO ...
-
bioRxiv - Immunology 2024Quote: ... 10X hi ovomucoid inhibitor stock solution contained 300 mg BSA (Sigma-Aldrich, #A8806), 300 mg ovomucoid trypsin inhibitor (Worthington ...
-
bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS, Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
-
bioRxiv - Microbiology 2024Quote: ... followed by western blotting was using anti-His primary antibody (Sigma-Aldrich, H1029) and anti-mouse IgG HRP conjugate (Promega ...
-
bioRxiv - Biochemistry 2024Quote: ... The medium was then loaded on a His-select Nickel affinity column (Millipore) equilibrated in 50 mM NaH2PO4 ...
-
bioRxiv - Molecular Biology 2021Quote: ... The HA-tagged PGBD1 was eluted by 500 μl 100 μg/ml HA-peptide (Sigma) in 100 mMNaCl ...
-
bioRxiv - Cell Biology 2019Quote: Immunoprecipitations of Flag-tagged proteins were performed using α-Flag M2 affinity beads (Sigma-Aldrich). For each immunoprecipitation reaction ...