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Citations for Millipore Sigma :
1701 - 1750 of 5383 citations for Recombinant Human Insulin Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... FLAG-tagged proteins were detected with Monoclonal ANTI-FLAG M2-Peroxidase (Sigma #A8592) at a dilution of 1:5,000 ...
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bioRxiv - Molecular Biology 2021Quote: ... Epitope tagged PARP1 was detected by mouse anti-FLAG antibody (# F3165, Millipore-Sigma) and visualized with an anti-mouse IgG coupled with Alexa Fluor 594 dye (Thermo Fisher Scientific # A-11005) ...
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bioRxiv - Cell Biology 2021Quote: ... EGFP-tagged TPX2 expressing hESCs with minimal dose of doxycycline (Sigma-Aldrich, #D9891) were selected using a cell sorter (BD FACSAria II) ...
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bioRxiv - Microbiology 2022Quote: ... GST-tagged proteins were purified under native conditions on gluthatione-agarose resin (Sigma). The recombinant proteins were then washed 3 times with Buffer A (100mM KCl ...
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bioRxiv - Biochemistry 2022Quote: ... and MBP-tagged and associated proteins were eluted in 60 mM maltose (Sigma) for 30 minutes on ice.
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bioRxiv - Cell Biology 2020Quote: The 6His-tagged-She1Cter expression plasmid for was constructed in pet28 vector (Novagen). The She1 C-terminal part coding for amino-acid 194 to 338 was cloned between the NdeI and XhoI site of the vector downstream of 6His and thrombin site.
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bioRxiv - Molecular Biology 2019Quote: ... Myc-tagged proteins were incubated with an anti-Myc primary antibody 9E10 (Millipore) at a 1:2000 dilution while tubulin was incubated with anti-Tubulin antibody B-5-1-2 (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... TAP-tagged proteins were detected with peroxidase–anti-peroxidase– soluble complex (P1291, Sigma). α-tubulin was detected with antibody T5168 (Sigma).
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bioRxiv - Cell Biology 2020Quote: ... Myc-tagged proteins were immunoprecipitated with 4AG Myc monoclonal agarose beads (EMD Millipore), washed three times in lysis buffer and boiled in 2X Laemmli reducing buffer (Bio-Rad) ...
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bioRxiv - Physiology 2020Quote: ... or mouse anti-S-tag (for S-tagged NTD, 1:1500, Sigma, SAB2702204) and rabbit anti-Nav1.5 (1:150 ...
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bioRxiv - Immunology 2020Quote: ... Flag-tagged proteins were immunoprecipitated with Anti-FLAG M2 Magnetic Beads (Sigma-Aldrich). For the endogenous interaction assay ...
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bioRxiv - Molecular Biology 2021Quote: ... Epitope tagged PARP1 was detected by mouse anti-FLAG antibody (# F3165, Millipore-Sigma) and visualized with an anti-mouse IgG coupled with Alexa Fluor 594 dye (Thermo Fisher Scientific # A-11005) ...
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bioRxiv - Immunology 2022Quote: ... GFP-tagged SAMHD1 proteins were immunoprecipitated with anti-SAMHD1 antibody (Sigma-Aldrich, SAB1400478) bound to Protein-A/G-Agarose (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... Flag-tagged proteins were bound to anti-FLAG M2 affinity gel (Sigma-Aldrich) for 1 h at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... FLAG-tagged proteins were detected using anti-FLAG M2 primary (Sigma-Aldrich, F1804) antibodies combined with anti-mouse-HRP secondary (Rockland ...
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bioRxiv - Plant Biology 2024Quote: ... HA-tagged proteins were detected by anti-HA Peroxidase high affinity antibody (Sigma). GFP was detected using a horseradish peroxidase-conjugated anti-GFP antibody (1:1.000 ...
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bioRxiv - Molecular Biology 2024Quote: ... Tagged proteins were detected by FLAG-HRP conjugated antibody (Sigma, A8592, 1:4000), mouse HA-probe antibody (Thermofisher ...
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bioRxiv - Cell Biology 2024Quote: ... FLAG-tagged protein was isolated with anti-FLAG M2 magnetic beads (Sigma, M8823) for 2 hr at 4LJ°C and eluted with 100 μg/ml 3x FLAG peptide ...
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bioRxiv - Developmental Biology 2024Quote: ... FLAG-tagged proteins were purified using anti-FLAG M2 Affinity agarose gel (Sigma) for >1h at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... at 4 °C for 40 minutes and filtered before purification of TlyA by sequential Ni2+-affinity (Cytiva HisTrap™ FF crude 1mL or manual His-column using Millipore Ni-NTA His-Bind® Resin) and gel filtration (Cytiva HiLoad™ 16/600 Superdex™ 75 ...
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bioRxiv - Developmental Biology 2020Quote: ... 1000 U/mL recombinant leukemia inhibitory factor (LIF, Millipore), and 0.3% BSA (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... active recombinant GSK3α was purchased (Millipore, Cat# 14-492). 0.8 µg Dyn2WT or Dyn2S848A was incubated with 10 ng GSK3α in kinase buffer (50 mM HEPES (pH 7.4) ...
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bioRxiv - Cell Biology 2021Quote: ... active recombinant GSK3α was purchased (Millipore, Cat# 14-492). 0.8 µg Dyn2WT or Dyn2S848A was incubated with 10 ng GSK3α in kinase buffer (50 mM HEPES (pH 7.4) ...
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bioRxiv - Bioengineering 2022Quote: ... Plates were coated with either recombinant GFP protein (Millipore) at 1 μg/mL to measure GFP-specific responses ...
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bioRxiv - Microbiology 2022Quote: ... recombinant IL-2 (100 U/mL; Sigma Millipore #11011456001), and anti-CD28 + anti-CD49d (1.5ug/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... and 2 μg/ml of recombinant TNC (Merck Millipore) added to the medium ...
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bioRxiv - Plant Biology 2021Quote: ... which yields recombinant proteins fused to hexahistidine tail (Novagen). The correct cloning was confirmed by sequencing.
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bioRxiv - Cell Biology 2021Quote: ... 1 μg of recombinant CDK1:CyclinB1 active complex (Millipore) in the presence or absence of 3 mM ATP ...
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bioRxiv - Cell Biology 2021Quote: ... 1 μg of recombinant CDK1:CyclinB1 active complex (Millipore) in the presence or absence of 3 mM ATP ...
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bioRxiv - Microbiology 2022Quote: ... recombinant IL-2 (100 U/mL; Sigma Millipore #11011456001), and anti-CD28 + anti-CD49d (1.5ug/ml ...
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bioRxiv - Biochemistry 2022Quote: ... 1,000 U/mL recombinant leukemia inhibitory factor (LIF, Millipore), and 0.3% BSA (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... recombinant mouse EGF (0·025 µg/ml, Sigma-Aldrich), and bovine pituitary extract (30 µg/ml ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... supplemented with 75 mIU/ml of recombinant FSH (Millipore), 100 mIU/ml recombinant hCG (Sigma) ...
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bioRxiv - Immunology 2024Quote: ... 20 μg of Ovalbumin recombinant protein (Sigma-Aldrich, A5503) (1:1 mixed with Alhydrogel ...
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bioRxiv - Biochemistry 2021Quote: ... The eluted solution of receptors was concentrated to 500 μL using 50 kDa spin filters (Millipore) and further purified by size exclusion chromatography on a Superdex 200 Increase 10/300 column (GE Healthcare ...
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bioRxiv - Developmental Biology 2021Quote: ... 100 ng/L ethinylestradiol (EE2) or 10μM ICI 182,780 (ICI, an estrogen receptor antagonist, Sigma-Aldrich)(aqueous exposure in system water ...
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bioRxiv - Neuroscience 2020Quote: ... and the AMPA receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 10 μM, Sigma Aldrich), for 2 min at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... 5 μM mecamylamine (Mec, relatively non-selective antagonist with higher affinity for β4 containing receptors, Sigma), 10 µM DHβE (α4β2* nAChR antagonist ...
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bioRxiv - Immunology 2022Quote: ... Immune mice sera samples were heat-inactivated and treated with receptor destroying enzyme (RDE, SIGMA-ALDRICH) before use ...
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bioRxiv - Microbiology 2020Quote: ... four volumes of receptor destroying enzyme (RDE, Vibrio cholera filtrate, Sigma Aldrich, San Diego, CA, USA) were added to each volume of mouse serum ...
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bioRxiv - Neuroscience 2021Quote: ... All experiments were performed in the presence of blockers of GABA receptors (picrotoxin, 30 μM, Sigma) and glutamate receptors (NBQX ...
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bioRxiv - Neuroscience 2022Quote: ... both receptors were nucleofected together with VAMP2-SEP and DAMGO 10μM or DPDPE 10μM (Sigma-Aldrich) added to the culture medium for 18h in the incubator before imaging ...
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bioRxiv - Neuroscience 2022Quote: ... while IPSCs were completely inhibited by the GABAA receptor antagonist bicuculline (Sigma-Aldrich, St. Louis, MO). To assess synaptic input from either or both POm and vM1 ...
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bioRxiv - Neuroscience 2022Quote: ... Following drugs were used for pharmacological manipulations: adrenergic α1 receptor agonist – phenylephrine (10 μM Sigma P6126); AMPA & Kainate receptors antagonist – CNQX (10 μM ...
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bioRxiv - Biochemistry 2023Quote: ... and probe the receptor using anti-FLAG peroxidase coupled antibody (1:2000, Sigma, Cat. no. A8592). Data were quantified using ImageLab software (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... and the postsynaptic AMPA receptors (Mouse IgG2B anti PAN-GluA1-4, 1:500; Millipore-Sigma: #MABN832). Coverslips were incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... and the postsynaptic AMPA receptors (Mouse IgG2B anti PAN-GluA1-4, 1:500; Millipore-Sigma: #MABN832). Coverslips were incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... 0,005 CPP [()-3-(2-carboxypiperazin-4-yl)propyl-1-phosphonic acid] (NMDA receptors antagonist, Sigma Aldrich). Traces of whole-cell voltage-clamp recordings (holding potential ...
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bioRxiv - Neuroscience 2024Quote: ... and before and 20–60 minutes after addition of GABA receptor antagonist bicuculline (10μM; Sigma-Aldrich).
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bioRxiv - Cell Biology 2020Quote: ... Penicillin-Streptomycin solution and bovine insulin were purchased from Sigma (St. Louis, MO, USA). Fetal bovine serum was purchased from Corning Inc ...