Labshake search
Citations for Millipore Sigma :
2051 - 2100 of 5383 citations for Recombinant Human Insulin Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2023Quote: ... human iPSC-derived cardiomyocytes were suspended in fibrinogen from human plasma (Sigma-Aldrich) at a concentration of 50x106 cells/ml ...
-
bioRxiv - Neuroscience 2019Quote: ... synaptic GABAA receptor currents were isolated pharmacologically with AMPA and NMDA-type glutamate receptor antagonists: 6,7 dinitroquinoxaline-2,3-dione (DNQX, 20 μM; Sigma Aldrich) and DL-2-amino-5-phosphonopentanoic acid (AP5 ...
-
bioRxiv - Neuroscience 2021Quote: ... Vasoconstriction was induced by the application of the thromboxane A2 receptor agonist U46619 (9,11-dideoxy-11a,9a-epoxymethanoprostaglandin F2α, 50 nM, Sigma) for 2 min ...
-
bioRxiv - Neuroscience 2022Quote: ... and NMDA receptor mediated PSCs were blocked with DL-2-amino-5-phosphonopentanoic acid (D-AP5; 50 μM; Sigma). The AMPA-R PSC frequency and amplitude was determined from a 1 min interval after the recording had stabilized (~10 min after wash-in of picrotoxin/D-AP5) ...
-
bioRxiv - Neuroscience 2021Quote: Rats were subcutaneously injected with NMDA receptor co-agonist D-SER (s.c. D-SER 10mg/Kg, Sigma-Aldrich, Ireland) in vehicle ...
-
bioRxiv - Biochemistry 2021Quote: ... Blot was stripped and re-probed for total receptor using HRP conjugated anti-FLAG M2-antibody (Sigma, 1: 5000). The signal was normalized with respect to total receptor and quantified using ImageLab software (BioRad).
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Effect of glucocorticoid receptor modulators on osteogenic differentiation of MSCs was quantified using Alizarin Red S (Sigma-Aldrich, A5533) staining ...
-
bioRxiv - Neuroscience 2021Quote: ... Vasoconstriction was induced by the application of the thromboxane A2 receptor agonist U46619 (9,11-dideoxy-11a,9a-epoxymethanoprostaglandin F2α, 50 nM, Sigma) for 2 min ...
-
bioRxiv - Neuroscience 2020Quote: The following neurotransmitter/neuropeptide blockers were used: CCK-A receptor antagonist devazepide ([2mg/kg] in 5% DMSO PBS; Sigma)10,41 ...
-
bioRxiv - Immunology 2021Quote: ... Diphtheria toxin receptor (DTR) and control mice were given 200 ng intraperitoneal injections of diphtheria toxin (Sigma Cat #D0564) on three consecutive days prior to transplant ...
-
bioRxiv - Physiology 2023Quote: ... mice were given the highly-selective ß3-adrenergic receptor agonist CL-316,243 (CL; Sigma; 1 mg/kg body mass) dissolved in saline ...
-
bioRxiv - Biochemistry 2023Quote: ... Solubilised receptors were harvested by centrifugation at 45,000 rpm for 1 hour and supplemented with 10 mM imidazole (Sigma) before loading onto a Nickel-NTA (Ni2+ ...
-
bioRxiv - Neuroscience 2023Quote: ... in cold Gey’s balanced salt solution (GBSS) supplemented with the glutamate receptor antagonist kynurenic acid (1 mM, Sigma, K3375) and glucose (33.33 mM ...
-
bioRxiv - Neuroscience 2024Quote: ... Activity was recorded before and 5–60 minutes after addition of NMDA receptor antagonist AP5 (50μM; Sigma-Aldrich, A8054), and before and 20–60 minutes after addition of GABA receptor antagonist bicuculline (10μM ...
-
bioRxiv - Plant Biology 2020Quote: ... using either 0.5 μg of recombinant histone H3 (Millipore, Cat. #14-411), 2 μg of chicken core histones (Millipore ...
-
bioRxiv - Molecular Biology 2021Quote: ... Recombinant proteins were extracted and purified using the Bugbuster reagent (Novagen, Inc). To raise antibodies against TRF4 or TIMRHOM1 ...
-
bioRxiv - Cell Biology 2020Quote: ... 14-3-3 antibody and active recombinant AMPK (α2β1γ1) were from Millipore. Precast Tris-Glycine polyacrylamide gels and 10X tris-glycine gel electrophoresis buffer were from BioRad ...
-
bioRxiv - Genetics 2020Quote: ... 0.5 U DNA recombinant Taq polymerase in buffer (pH= 8.0; Sigma, Germany), 1x PCR buffer (pH=8.7 ...
-
bioRxiv - Bioengineering 2021Quote: ... and 1 ng/mL of recombinant basic fibroblast growth factor (Sigma-Aldrich). RAW 264.7 macrophages were cultured in complete media composed of high-glucose Dulbecco’s Modified Eagle’s Medium (DMEM (1x ...
-
bioRxiv - Bioengineering 2021Quote: ... Supernatant containing soluble recombinant protein was loaded into α-lactose agarose (Sigma) affinity column preequilibrated with PBS ...
-
bioRxiv - Biochemistry 2021Quote: ... Bovine erythrocyte ubiquitin and recombinant hsNEDD8 were purchased from Sigma-Aldrich (U6253) and BostonBiochem (UL-812 ...
-
bioRxiv - Microbiology 2020Quote: ... A set of recombinant RT standards (EMD Millipore, Molsheim, France; catalog# 382129) ranging from 5.12e3 nU/mL to 5.12e9 nU/mL was used to estimate the nU/mL of reverse transcriptase activity in the FIV stock.
-
bioRxiv - Microbiology 2021Quote: ... Recombinant VEGF (V7259) and TGF-β1 (100-21) was purchased from Sigma and PeproTech ...
-
bioRxiv - Microbiology 2020Quote: ... The Red/ET recombinant enzyme was induced by L-arabinose (Sigma-Aldrich) and a temperature shift from 30°C to 37°C ...
-
Q-SNARE Syntaxin 7 confers actin-dependent rapidly replenishing synaptic vesicles upon high activitybioRxiv - Neuroscience 2020Quote: ... neurons were incubated with 10 nM recombinant TeNT–light chain (Sigma–Aldrich) for 16 h in a CO2 incubator ...
-
bioRxiv - Cell Biology 2020Quote: ... The recombinant protein was expressed in Rossetta2(DE3) strain (EMD Millipore/Novagen). Expressed protein was found in inclusion body thus the proteins were solubilized by 8M urea containing buffer (20mM Hepes pH7.8 ...
-
bioRxiv - Cell Biology 2020Quote: ... The recombinant protein was expressed in Rossetta2(DE3) strain (EMD Millipore/Novagen). Expressed protein was found in inclusion body thus the proteins were solubilized by 8M urea containing buffer (20mM Hepes pH7.8 ...
-
bioRxiv - Cell Biology 2019Quote: ... The recombinant protein was expressed in Rossetta2(DE3) strain (EMD Millipore/Novagen). Expressed fragment was found in inclusion body thus the proteins were solubilized by 8M urea containing buffer (20mM Hepes pH7.8 ...
-
bioRxiv - Cell Biology 2019Quote: ... The recombinant protein was expressed in Rossetta2(DE3) strain (EMD Millipore/Novagen). Expressed fragment was found in inclusion body thus the proteins were solubilized by 8M urea containing buffer (20mM Hepes pH7.8 ...
-
bioRxiv - Molecular Biology 2020Quote: ... 3 μM CHIR99021 (Cayman) and 1,000 U/ml recombinant mouse LIF (Millipore)39 ...
-
bioRxiv - Biochemistry 2021Quote: ... and supplied with 1000 U/ml recombinant leukemia inhibitory factor (LIF, Millipore). 293T cells were cultured in DMEM supplemented with 10% FCS.
-
bioRxiv - Biochemistry 2021Quote: ... and supplied with 1000 U/ml recombinant leukemia inhibitory factor (LIF, Millipore). ESCs were cultured on plates which were pre-coated with 0.1% gelatin ...
-
bioRxiv - Genetics 2020Quote: ... 10 ng/μL recombinant fibroblast growth factor basic (βFGF; Sigma-Aldrich, #F0291), 10 μg/μL Heparin (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2020Quote: ... supplemented with 1000 units/ml ESGRO Recombinant Mouse LIF protein (Millipore #ESG1107), 10% fetal bovine serum ...
-
bioRxiv - Synthetic Biology 2021Quote: Recombinant hACE2 with a C-terminal deca-histidine tag (SAE0064, Sigma-Aldrich) was resuspended in PBS to a final concentration of 50 ng/μL ...
-
bioRxiv - Molecular Biology 2021Quote: ... samples were digested overnight at 37 °C with recombinant trypsin (Trypzean, Sigma) (enzyme ...
-
bioRxiv - Immunology 2021Quote: ... Recombinant Wnt5A (Cat no.- 10UG 645-WN-010 R & D, GF146 Millipore) and recombinant Wnt3A (Cat no.- 10UG 5036-WN-010 R & D ...
-
bioRxiv - Cancer Biology 2022Quote: ... eluted in water and aliquoted for later use with recombinant Cas9 (Sigma). Oligos used for sgRNA production are listed in Extended Data Table 4.
-
ZMYM2 is essential for methylation of germline genes and active transposons in embryonic developmentbioRxiv - Molecular Biology 2022Quote: ... media supplemented with 1000U/mL ESGRO Recombinant Mouse LIF (Millipore Sigma, ESG1107), 2 mM GlutaMAX (Gibco ...
-
bioRxiv - Immunology 2023Quote: ... then tested against recombinant proteins coated in Dulbecco’s PBS (DPBS, Sigma-Aldrich) overnight onto 96-well Immunlon-2 plates (VWR Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... and with recombinant mouse Leukemia Inhibitory Factor (LIF, 20 U/ml, Millipore).
-
bioRxiv - Molecular Biology 2023Quote: ... The DNA was digested by adding 10 U DNase I recombinant (Sigma) and incubating for one additional hour ...
-
bioRxiv - Biochemistry 2023Quote: ... They were incubated with recombinant ULK1 proteins (Sigma, #SRP5096-10UG, Darmstadt, Germany) in the kinase buffer (20 mM HEPES (pH 7.5) ...
-
bioRxiv - Developmental Biology 2023Quote: ... and 10 μg/μl recombinant mouse leukemia inhibitory factor (LIF; Sigma-Aldrich) in a 5% CO2 incubator at 37°C ...
-
bioRxiv - Developmental Biology 2024Quote: ... Serial dilutions from 2μg to 0.0625μg of recombinant SPARC (SRP3159, Sigma-Aldrich; S5174-25UG ...
-
bioRxiv - Cell Biology 2021Quote: ... then switched to differentiation medium (1.5% FBS, 1μg/ml bovine insulin, 1μM hydrocortisone, 10μM trans-retinoic acid (Sigma®), and 5U brain-derived neurotropic factor (BDNF ...
-
bioRxiv - Neuroscience 2020Quote: ... Japan) in growth factor-free chemically-defined medium in the presence of 7μg/ml Insulin (Sigma, USA), 50 μM Y-27562 (Tocris ...
-
bioRxiv - Pathology 2019Quote: ... Sections were co-stained with primary antibodies against insulin (guinea pig; 1:500 and 1:1000; Millipore) and proinsulin (mouse ...
-
bioRxiv - Developmental Biology 2019Quote: ... 1 μg/ml insulin and 0.5mM methylisobutyl-xanthine in 10% Fetal Bovine Serum(FBS)/Dulbecco’s Modified Eagle’s Medium media (Sigma). Cells were fed with fresh media every two days ...
-
bioRxiv - Physiology 2019Quote: Mouse serum insulin levels were determined using the mouse metabolic hormone panel (Millipore Sigma, Milliplex MMHMAG-44K) according to the manufacturer’s instructions ...