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Citations for Millipore Sigma :
1751 - 1800 of 5383 citations for Recombinant Human Insulin Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... the animals were subjected to ip injection of 1 U of insulin (Sigma Aldrich) per kg body weight ...
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bioRxiv - Biochemistry 2019Quote: ... All the buffer and insulin solutions were sterilized with a 0.22-μm filter (Millipore). Insulin concentration was measured by UV absorption at 276 nm by using an extinction coefficient of 1.0675 cm−1 (mg/ml ...
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bioRxiv - Physiology 2021Quote: ... and plasma insulin (pg/ml) was measured via Luminex multiplex technology (Millipore, Billerica, MA).
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bioRxiv - Cell Biology 2020Quote: ... or DMEM (4.5g/L glucose) + 20% FBS + 1μg/ml Insulin (Cat nb. 91077C, Sigma) + 0.25 μM Dexamethasone (Cat nb ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the medium was changed to DMEM supplemented with insulin/transferrin/selenium (Sigma-Aldrich, I3146) and cultures were used within 5 days ...
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bioRxiv - Developmental Biology 2022Quote: ... LIF and the additional insulin) supplemented with 0.25μM all-trans retinoic acid (Sigma-Aldrich). Medium was being refreshed every 48H.
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bioRxiv - Cell Biology 2022Quote: ... for 15 min and/or 100 nM insulin (I0516, Sigma-Aldrich, St. Louis, MO) for 10 min ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were then collected after 16 hours with or without insulin (Sigma I9278-5ML) (100 nM ...
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bioRxiv - Neuroscience 2020Quote: ... and N1 medium (high glucose DMEM supplemented with 5 μg/ml insulin (#I6634, Sigma), 50 μg/ml apo-transferrin (#T2036 ...
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bioRxiv - Immunology 2022Quote: ... and 1% insulin transferrin sodium selenite (ITS) liquid media supplement (Sigma-Aldrich, ON, Canada) and incubated at 37°C/5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... 1 μg/mL insulin and 20% Horse Serum (heat inactivated, Millipore-Sigma, Burlington, Massachusetts), pH 7.45 and 305 mOsm) ...
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bioRxiv - Neuroscience 2023Quote: ... 1 μg/mL insulin and 20% Horse Serum (heat inactivated, Millipore-Sigma, Burlington, Massachusetts), pH 7.45 and 305 mOsm) ...
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bioRxiv - Neuroscience 2023Quote: ... 1 μg/mL insulin and 20% Horse Serum (heat-inactivated, Millipore-Sigma, Burlington, Massachusetts), pH 7.45 and 305 mOsm) ...
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bioRxiv - Neuroscience 2023Quote: ... 1 μg/mL insulin and 20% Horse Serum (heat-inactivated, Millipore-Sigma, Burlington, Massachusetts), pH 7.45 and 305 mOsm) ...
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bioRxiv - Neuroscience 2023Quote: ... 1 μg/mL insulin and 20% Horse Serum (heat inactivated, Millipore-Sigma, Burlington, Massachusetts), pH 7.45 and 305 mOsm) ...
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bioRxiv - Neuroscience 2023Quote: ... 1 μg/mL insulin and 20% Horse Serum (heat inactivated, Millipore-Sigma, Burlington, Massachusetts), pH 7.45 and 305 mOsm) ...
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bioRxiv - Cancer Biology 2022Quote: ... Bovine pancreas insulin solution was purchased from Millipore Sigma (Cat# I0516; Burlington, MA, USA). Recombinant human EGF was purchased from Peprotech (Cranbury ...
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bioRxiv - Microbiology 2023Quote: ... IEC-6 cells were supplemented with 0.1 U/mL of bovine insulin (Sigma-Aldrich). The cells were seeded in 8- chambers slides or in 96-wells plates (Falcon ...
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bioRxiv - Cell Biology 2024Quote: ... b) Minimal Induction (MI) (Hilgendorf et al., 2019) containing 0.4 µg/ml insulin (Sigma), 0.1 µM Dexamethasone (Sigma) ...
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bioRxiv - Neuroscience 2024Quote: ... and N1 medium (high-glucose DMEM supplemented with 5 μg/ml insulin (#I6634, Sigma), 50 μg/ml apo-transferrin (#T2036 ...
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bioRxiv - Biophysics 2024Quote: The insulin-releasing pancreatic β-cell line (1.1B4, Merck KGaA, Sigma Aldrich, Darmstadt, Germany) was cultured in a 175 cm2 cell culture bottles and maintained in RPMI 1640 medium (cat ...
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bioRxiv - Biophysics 2024Quote: ... Insulin from bovine pancreas was purchased as a lyophilized powder (Sigma cat. no. I5500) and used without further purification ...
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bioRxiv - Biochemistry 2022Quote: ... as a His-tag fusion and Ars2206-531 into pRSFduet vector (Novagen) as a Strep-tag fusion ...
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bioRxiv - Molecular Biology 2019Quote: ... Monoclonal anti-His and anti-Flag antibodies were obtained from Sigma Aldrich, St ...
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bioRxiv - Microbiology 2019Quote: ... The supernatant was mixed with Ni2+-NTA His•Bind resin (EMD Millipore) equilibrated with Buffer G and allowed to go overnight at 4 °C ...
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bioRxiv - Microbiology 2019Quote: ... The supernatant was mixed with Ni2+-NTA His•Bind resin (EMD Millipore) equilibrated with Lysis/Binding Buffer (50 mM Na2HPO4 ...
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bioRxiv - Microbiology 2020Quote: ... and proteins were purified over HIS-Select (Sigma–Aldrich, St. Louis, MO) nickel affinity resin in 20 mM Tris (pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... 10 % heat-inactivated fetal bovine serum (HI-FBS) (v/v) (Sigma-Aldrich), and 1 % antibiotic-antimycotic (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... The supernatant was loaded onto His-Select Nickel Affinity Gel (Sigma-Aldrich) equilibrated with buffer A (25 mM Tris–HCl ...
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bioRxiv - Biochemistry 2021Quote: ... Bound protein was detected using anti-His-HRP conjugate (Sigma; 1:10,000). All proteins and antibodies were in 0.1 M phosphate buffer ...
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bioRxiv - Cell Biology 2021Quote: ... The β-Spectrin-His construct was transformed into BL21(DE3) cells (Novagen) and induced for protein expression with 0.5mM IPTG (Invitrogen ...
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bioRxiv - Immunology 2022Quote: 30µL/well of rabbit anti-His tag antibody (Sigma Aldrich, catalog #SAB5600227) was adsorbed at a concentration of 0.5 μg/mL on to 384 well microplates (Thermofisher ...
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bioRxiv - Bioengineering 2022Quote: ... Pre-cleared supernatant was mixed with Ni-NTA His-Bind Resin (Novagen) (Pajenda et al. ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 15% FBS HI (Life Tehnologies) + 1% Pen/Strept (Sigma-Aldrich), 1% L-glutamine (Life Technologies) ...
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bioRxiv - Molecular Biology 2019Quote: ... Purification of rcCBP2 was performed by HIS-Select HF Nickel Affinity (Sigma) and gel permeation chromatography on Superdex 75 column (GE Healthcare Life Sciences) ...
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bioRxiv - Cell Biology 2019Quote: ... for 10 minutes followed by anti-his antibody (Sigma, Cat. No. 11922416001) for another 10 mins ...
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bioRxiv - Microbiology 2021Quote: ... The crude lysate rocked with HIS-select nickel affinity gel resin (Sigma) for 45 min at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... the supernatant was loaded onto a His-bind resin column (Merck Millipore) (1 ml ...
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bioRxiv - Plant Biology 2023Quote: ... The membrane was probed with 1:10000 anti-His antibody (Sigma, USA) followed by 1:20000 anti-mouse antibody (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... The His-tag of the eluted protein was cleaved by thrombin (Sigma), incubated at 4 °C overnight ...
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bioRxiv - Biophysics 2023Quote: ... His-Clathrin was labeled with Atto594 NHS-ester (ATTO-TEC, Sigma-Aldrich) according to a previously published protocol (41 ...
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bioRxiv - Plant Biology 2023Quote: ... in-frame of the C-terminal (His)6-tag (Novagen, http://www.emdbiosciences.com) resulting in pET28a:ApTPS1 ...
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bioRxiv - Biophysics 2023Quote: ... then incubated with mouse anti-His tag primary antibody (Millipore Sigma SAB1305538) at 1:1,000 dilution ...
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bioRxiv - Plant Biology 2024Quote: ... and confirmed by Western blotting using His-tag antibody (Sigma; SAB 1305538). The His-tagged GUN1-PS protein was produced essentially as described (19) ...
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bioRxiv - Immunology 2024Quote: ... and 5% Fetal bovine serum (FBS; Sigma Heat Inactivated, US/HI origin). PBMCs were cultured overnight in chemotaxis media containing RPMI and 1% ultrapure BSA prior to use in assays.
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS) (Sigma Aldrich) at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... or human liver microsomes (HLMs) (male human pooled, Sigma-Aldrich), respectively ...
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bioRxiv - Microbiology 2020Quote: ... FLAG-tagged ANP32 proteins were detected using mouse anti-FLAG M2 primary antibody (Sigma), followed by goat anti-mouse Alexa Fluor 568 (Invitrogen) ...
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Transcription factor LSF facilitiates lysine methylation of α-tubulin by microtubule-associated SET8bioRxiv - Biochemistry 2019Quote: ... the epitope tagged LSF was detected by mouse anti-FLAG antibody (F3165, Sigma-Aldrich) and visualized with an anti-mouse IgG coupled with Alexa Fluor 488 (Molecular Probes) ...
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bioRxiv - Biochemistry 2020Quote: ... Flag-EGFP tagged ATP11C was eluted with 0.2 mg/ml Flag peptide (Sigma-Aldrich) in the wash buffer ...