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Citations for Millipore Sigma :
1451 - 1500 of 10000+ citations for HLA A*0201 WT 1 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... the membrane was incubated with polyclonal rabbit antibodies recognizing human PLG (Sigma- Aldrich, 1:500), followed by peroxidase-conjugated secondary antibodies (1:10,000) ...
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bioRxiv - Immunology 2023Quote: ... Human acute monocytic leukemia cell line (THP-1) was cultured in RPMI-1640 (Sigma-Aldrich) supplemented with 10% heat-inactivated fetal calf serum (FCS ...
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bioRxiv - Neuroscience 2024Quote: ... sections were double-immunolabeled for human nuclei and either Htt (1:200; clone EM48, Millipore) or IBA-1 (1:1,000 ...
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bioRxiv - Plant Biology 2019Quote: ... the membrane was incubated with anti-6x His antibody horseradish peroxidase (HRP) conjugate (Sigma A7058-1VL ...
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bioRxiv - Molecular Biology 2019Quote: ... Supernatant containing 20mM imidazole was incubated with Ni-NTA His•Bind® Resin (Novagen) beads with end-to-end shaking for 4 hr at 4°C ...
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bioRxiv - Immunology 2021Quote: ... the His-tag was cleaved using a Thrombin kit (Millipore Sigma, St. Louis, MO). Confirmation of the His-tag removal was confirmed by W ...
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bioRxiv - Microbiology 2021Quote: ... Cleared lysates were loaded on chromatographic columns filed with His-select resin (Millipore Sigma) or glutathione resin (GE healthcare) ...
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bioRxiv - Biochemistry 2022Quote: ... Ni-NTA pull down was conducted by using HIS-Select Nickel Affinity Gels (Sigma). After lysing cell pellet with buffer A (6 M guanidine-HCl ...
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bioRxiv - Plant Biology 2021Quote: ... Western blotting was performed using anti His-tag (Cat no. H1029-5ML, Sigma, USA) as well as with anti GST antibody (Cat no ...
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bioRxiv - Plant Biology 2020Quote: ... The pET-26-tNPR1-His plasmid was then transformed into Rosetta (DE3) pLysS (Novagen). The overnight culture at 37°C was transferred to fresh LB medium containing 50 μg/mL kanamycin and was incubated to OD600=1.0 at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS) (v/v) (Sigma-Aldrich) and 1% antibiotic-antimycotic (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... and the supernatant was applied to a HIS-Select Nickel Affinity Gel column (Sigma) equilibrated in lysis buffer ...
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bioRxiv - Cell Biology 2021Quote: ... and the supernatant was applied to a HIS-Select Nickel Affinity Gel column (Sigma) equilibrated in lysis buffer ...
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bioRxiv - Bioengineering 2022Quote: ... Primary antibody (Anti-His; Invitrogen, Waltham, MA or Anti-HA; Sigma Aldrich, St.Louis, MO) was added to the blocking solution at 1:2,000 dilution and incubated overnight at 4 °C ...
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bioRxiv - Plant Biology 2022Quote: ... and the subsequent western blot analyses were performed with anti-His (Sigma, Missouri, USA) or anti-FLAG antibody (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... The His-tag was removed by O/N incubation with TEV protease (Millipore Sigma) at 4 °C followed by negative INAC ...
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bioRxiv - Immunology 2020Quote: ... and loaded onto a cOmplete™ His-Tag Purification Resin gravity column (Sigma Millipore). Resin was first washed with detergent-containing buffer (25 mM Tris + 500 mM NaCl + 0.5 % N-Dodecyl-β-D-maltoside ...
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bioRxiv - Immunology 2022Quote: ... The lysate was mixed gently with 50% Ni-NTA His-bind slurry (EMD Millipore) at 4:1 ratio on a shaking platform for 60 minutes at RT ...
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bioRxiv - Cell Biology 2019Quote: ... input was taken and 20 µl HIS-Select® HF Nickel Affinity Gel (Sigma) pre-equilibrated with Pull-Down Buffer was added to each sample and incubated on an overhead rotator for 1h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... and the supernatant was incubated with 6 ml HIS-Select Nickel Affinity Gel (Sigma), gently agitated at 4°C for 1 h ...
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bioRxiv - Immunology 2020Quote: ... BM cells were cultured in growth media supplemented with 10% HI FBS (Sigma-Aldrich), 1% Pen/Strep (Lonza) ...
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bioRxiv - Immunology 2020Quote: ... and loaded onto a cOmplete™ His-Tag Purification Resin gravity column (Sigma Millipore). Resin was first washed with detergent-containing buffer (25 mM Tris + 500 mM NaCl + 0.5 % N-Dodecyl-β-D-maltoside ...
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bioRxiv - Biochemistry 2021Quote: ... the His-tag was removed by treating purified nanobody Nb_0 with carboxypeptidase A (Sigma) and B (Roche ...
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bioRxiv - Molecular Biology 2022Quote: ... Expression of pAgos was verified by Western blot with antibodies against His-tag (Sigma) after cell growth for 2 hours at 30 °C ...
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bioRxiv - Bioengineering 2022Quote: ... and mVenus-SpyCatcher-6×His were prepared using Gibson assembly with pET-19b (Novagen) as the plasmid vector ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Mpp75Aa1.1 or Mpp75Aa1.1-His was mixed with high purity pepsin (Sigma, St. Louis, MO) in 2 mg/ml NaCl ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% heat-inactivated Fetal Bovine Serum (FBS-hi; Sigma, Cat. no. F7524), 1% antibiotic-antimycotic (AA ...
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bioRxiv - Molecular Biology 2024Quote: ... The cleared lysate was added to cOmplete His-Tag Purification Resin (Merck Millipore/Roche) and incubated at 4°C for 1.5 h ...
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bioRxiv - Biochemistry 2023Quote: ... recombinant insulin human (≥ 98%), somatostatin-14 (≥ 97%, human HPLC grade), and urocortin-3 (≥ 97%, human HPLC grade) were all purchased from Sigma-Aldrich. Taxonomy will only be indicated for insulin ...
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bioRxiv - Microbiology 2023Quote: ... Cells that received Interferon-β pre-treatment were stimulated for 18 hours with 100 units of recombinant human Interferon-β protein (Millipore, IF014) prior to infection.
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bioRxiv - Immunology 2019Quote: ... endogenous Asc from a total of 3.5 mg protein was immunoprecipitated with 1 μg of anti-Asc antibody and 60 μl of protein A/G agarose beads (Sigma), and bound Mul1 was detected with anti-Mul1 antibody by western blotting ...
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bioRxiv - Biophysics 2021Quote: ... The GB1 tag was cleaved from the oxidized proteins by incubating overnight with thrombin (10 U.mg−1 protein, Sigma-Aldrich). The cleaved protein was purified by reversed phase high-performance liquid chromatography (RP-HPLC ...
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bioRxiv - Biophysics 2020Quote: ... For reduction and alkylation of the proteins, proteins were incubated with SDC buffer (1% Sodiumdeoxycholate, 40nmM 2-Cloroacetamide (Sigma-Aldrich), 10 mM tris(2-carboxyethyl ...
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bioRxiv - Microbiology 2021Quote: ... The following antibodies were used for specific protein detection: anti-PAP (1: 1:5000, rabbit, Sigma), anti-myc (mouse ...
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bioRxiv - Biochemistry 2020Quote: ... and protein expression was induced with 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) (Sigma-Aldrich) for 20-24h at 20°C ...
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bioRxiv - Cell Biology 2021Quote: ... eosinophil peroxidase (EPX) and Fibroblast specific protein-1 (FSP-1) were procured from Sigma-Aldrich (Merk). All other important reagents were procured from sigma other than specified Sigma-Aldrich (Merk).
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bioRxiv - Molecular Biology 2024Quote: ... the protein expression was induced by 1 mM isopropyl 1-thio-β-D-galactopyranoside (IPTG, Sigma) at 16°C overnight ...
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bioRxiv - Microbiology 2024Quote: ... 1% BSA at 4 °C overnight: rabbit anti-microtubule-associated protein 2 (MAP2, Millipore 1: 200); sheep polyclonal anti-dopamine beta hydroxylase (DBH ...
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bioRxiv - Cell Biology 2020Quote: ∼1mg of purified Rab1/Ypt1 (either prenylated-Ypt1/GDI complex or non-prenylated Ypt1-7xHis) was incubated in the presence of 20µM mantGDP (Sigma) and EDTA to facilitate exchange for 30 minutes at 30°C ...
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bioRxiv - Biophysics 2019Quote: ... The BBSome:ARL6 complex was eluted from the column with a total of 10 ml of 0.1 mg/ml Flag peptide (Sigma). Elution was performed in 5 steps ...
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bioRxiv - Biochemistry 2020Quote: ... After formation of the CAK-MAT1Δ219-THZ1 complex by incubation of 0.2 mg/mL CAK-MAT1Δ219 (approx. 2 μM) with 5 μM THZ1 (EMD Millipore) for 1.5 h at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA-calcium phosphate transfection complex was prepared by gently adding plasmid DNA and 2M CaCl2 (Sigma-Aldrich; Merck KGaA) dropwise to HBS buffer (Sigma-Aldrich ...
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bioRxiv - Biophysics 2022Quote: ... Eluted fractions corresponding to the full ternary complex were gathered and spun concentrated to 14.5 mg/mL using an Amicon Ultrafree 10K NMWL Membrane Concentrator (Millipore). Crystals were grown 1-3 µL hanging drops by varying the ratio of protein to mother liquor from 0.5-2:0.5-2 respectively ...
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bioRxiv - Immunology 2020Quote: ... Fractions containing the complex were concentrated to 13 mg/mL using a 100 kDa Amicon® spin concentrator (Millipore).
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bioRxiv - Immunology 2020Quote: ... Fractions containing the complex were concentrated to 6 mg/mL using a 10 kDa Amicon® spin concentrator (Millipore). 3 μL of the complex was mixed with 1 μL of a n-Dodecyl-β-D-Maltopyranoside (DDM ...
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bioRxiv - Immunology 2020Quote: ... Fractions containing the complex were concentrated to 6.1 mg/mL using a 10 kDa Amicon® spin concentrator (Millipore). 3.5 μL of the complex was mixed with 0.57 μL of 0.04 mM lauryl maltose neopentyl glycol (LMNG ...
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bioRxiv - Immunology 2020Quote: ... Fractions containing the complex were concentrated to 4 mg/mL using a 10 kDa Amicon® spin concentrator (Millipore). 3 μL of the complex was mixed with 1 μL of a n-Dodecyl-β-D-Maltopyranoside (DDM ...
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bioRxiv - Biophysics 2022Quote: Peak fractions of the PEAK3/14-3-3 complex were pooled and concentrated using an Amicon Ultra-0.5 30k MWCO centrifugal filter (Millipore). Immediately before preparing cryo-EM grids ...
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bioRxiv - Microbiology 2022Quote: SEC-purified LCMV pfGP-TD and LCMV pfGP-TD/M28 Fab complex in TBS was concentrated to 0.8 and 1.2mg/mL using a 30K Amicon centrifugal filter (Sigma Aldrich). For LCMV pfGP-TDapo ...
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bioRxiv - Molecular Biology 2024Quote: ... and the FANCI-FANCD2 complex was eluted with FLAG wash buffer containing 100 µg/mL 3X FLAG peptide (Sigma) and 5% glycerol ...