Labshake search
Citations for Millipore Sigma :
1451 - 1500 of 10000+ citations for Recombinant Human CTLA4 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2024Quote: ... Human thrombin (Sigma, #T6884-100UN) was reconstituted in sterile 0.9% saline supplemented with human serum albumin (0.1% w/v ...
-
bioRxiv - Cancer Biology 2024Quote: ... thrombin (from human plasma, Sigma) and aprotinin (from bovine lung ...
-
bioRxiv - Immunology 2024Quote: ... 5% human AB serum (Sigma), 500 IU/mL of IL-2 (Proleukin S ...
-
bioRxiv - Pathology 2020Quote: ... high-purity (>99%) human CRP obtained from human plasma (C4063; Sigma-Aldrich, Saint Louis, Missouri) was infused via the femoral vein after 45 minutes of ischemia with LAD ligation ...
-
bioRxiv - Biophysics 2020Quote: ... Human Serum from human male AB plasma and NaOH 1N Bioreagent were purchased from Sigma. ss M13mp18 ...
-
bioRxiv - Immunology 2023Quote: ... Human platelets were stimulated with 1 U ml−1 Thrombin from human plasma (Sigma-Aldrich) or left unstimulated for 30 min to assess the purity and activation of isolated cells ...
-
bioRxiv - Biochemistry 2024Quote: ... and human apo-transferrin (P02787) isolated from human plasma were purchased from Sigma Aldrich (E0518), Invitrogen (RP-43132) ...
-
bioRxiv - Biochemistry 2022Quote: ... the biotinylated surface was coated for 10 min with 0.2 mg/mL Streptomyces avidinii streptavidin (Sigma-Aldrich) in PBS ...
-
bioRxiv - Neuroscience 2019Quote: ... Tissue was then incubated in secondary biotinylated rabbit antibody to goat antibody (1:250, Sigma, B-7024) for 2 h ...
-
bioRxiv - Biophysics 2021Quote: ... DDS-Tween 20 passivated surface (42) with 0.2 mg/mL biotinylated bovine serum albumin (A8549, Sigma-Aldrich) and 0.2 mg/mL NeutrAvidin (31000 ...
-
bioRxiv - Plant Biology 2022Quote: ... Biotinylated DNA fragments were purified following the NucleoSpin Gel clean-up kit (Sigma-Aldrich, catalog No. 740609.50) protocol ...
-
bioRxiv - Biochemistry 2024Quote: ... the surface was incubated for 10 min with biotinylated BSA (Sigma-Aldrich, 3 mg/ml in PBS), washed several times with PBS ...
-
bioRxiv - Microbiology 2024Quote: ... Samples were incubated with a biotinylated version of the lectin from Dolichos biflorus (Sigma Aldrich, #L6533-5MG) at 1:300 ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were biotinylated by incubation in biotin-free growth media supplemented with 50 µM biotin (Sigma-Aldrich). After exactly 20 minutes ...
-
bioRxiv - Biochemistry 2021Quote: ... The supernatant pH was adjusted to 8.0 and incubated with Ni2+-NTA His-binding resin (Novagen) that was pre-equilibrated with binding buffer (50 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2021Quote: ... the blot was incubated with a mouse anti-His-tag primary antibody (Sigma-Aldrich, Steinheim, Germany), diluted 1:1 000 in TBS (10 mM TRIS/HCl (pH 7.5) ...
-
bioRxiv - Biochemistry 2020Quote: ... The Trx-His(6) tag was cleaved by addition of 300 units of thrombin (Sigma T4648) directly to the eluate and the solution dialyzed (Slide-A-Lyzer dialysis cassettes ...
-
bioRxiv - Biophysics 2020Quote: ... MBP and the His-tag were cleaved from the P domain using HRV 3C protease (Novagen). Cleaved P domain protein eluted from Ni-NTA resin and was further purified by size-exclusion chromatography using a Superdex 16/600 200 pg column (GE Healthcare ...
-
bioRxiv - Neuroscience 2019Quote: ... or combined together (GPA2-FLAG/GPB5-His) and then treated with DSS (Sigma Aldrich, Oakville, ON). In some experiments ...
-
bioRxiv - Immunology 2021Quote: ... using cross-reactive anti-SARS-CoV-1 Spike CR3022 or mouse anti-His tag (Sigma-Aldrich) as positive controls ...
-
bioRxiv - Plant Biology 2021Quote: ... and GST were fused with a C-terminal octa-His tag in the pET42 vector (Novagen).
-
bioRxiv - Biochemistry 2021Quote: ... The latter were then probed with mouse anti-His HRP conjugated antibody (1:400, Sigma-Aldrich) and visualized by chromogenic DAB reagent (Sigma).
-
bioRxiv - Molecular Biology 2022Quote: ... Cleared supernatant was incubated with 20 μl His Mag Sepharose Ni beads (Sigma, GE28-9673-88) overnight ...
-
bioRxiv - Neuroscience 2024Quote: ... and the supernatant subjected to Ni-NTA affinity purification (HIS-Select Nickel Affinity Gel (Sigma Aldrich)) ...
-
bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifugation and applied to cOmplete His-tag purification resin (Sigma Aldrich) for 1 hour at 4 °C with rotation ...
-
bioRxiv - Molecular Biology 2024Quote: ... Clarified lysate was applied to 2.5 ml bed volume His-select Cobalt Affinity Gel (Sigma-Aldrich). The slurry was rotated in the cold room for an hour prior to application to a gravity flow column ...
-
bioRxiv - Developmental Biology 2021Quote: ... All additional recombinant genes were cloned into pFLAG-CMVTM-6a expression vector (Sigma) unless otherwise specified ...
-
bioRxiv - Genetics 2020Quote: ... 200 µg/ml Proteinase K (Proteinase K, recombinant, PCR Grade, 3115879001, Sigma Aldrich)) ...
-
bioRxiv - Molecular Biology 2021Quote: ... and 103 U/mL ESGRO recombinant mouse leukemia inhibitory factor (LIF) (Millipore; ESG1107) in an humidified cell incubator with 5% CO2 at 37°C.
-
bioRxiv - Biophysics 2020Quote: The recombinant MdmX (18-111 aa) was cloned into pET46Ek/LIC vector (Novagen) and expressed in E.coli BL21 (DE3 ...
-
bioRxiv - Cell Biology 2021Quote: ... Recombinant SBF or MBF complex was purified with anti-FLAG resin (M2, Sigma) from insect cells co-infected with FLAGSwi4-Swi6 or FLAGMbpl-Swi6 baculovirus constructs ...
-
bioRxiv - Microbiology 2021Quote: ... according to the manufacturer’s protocol and using recombinant porcine LDH enzyme (Sigma-Aldrich) for derivation of a standard curve ...
-
bioRxiv - Cell Biology 2022Quote: ... Recombinant PRDX6 was eluted with lysis buffer supplemented with 250 mM imidazole (Sigma). Subsequently ...
-
bioRxiv - Immunology 2020Quote: ... recombinant GM-CSF (10ng/ml; Peprotec) and β-estradiol (1 μM; Sigma-Aldrich). To differentiate into macrophages ...
-
bioRxiv - Microbiology 2020Quote: ... Recombinant genes were expressed in Escherichia coli strains BL21 (DE3) or TUNER (Novagen), containing the appropriate recombinant plasmid ...
-
bioRxiv - Developmental Biology 2021Quote: ... We injected in each matrix 25 μg of Recombinant Sema3C-Fc (Sigma-Aldrich) in a concentration of 50 μg/ml in PBS ...
-
bioRxiv - Cell Biology 2019Quote: ... Purified recombinant active TGF-β1 was purchased from Sigma Aldrich (St. Louis, MO). Immunofluorescence imaging was conducted using the following primary antibodies ...
-
bioRxiv - Cancer Biology 2021Quote: ... or C1-27 was added to 0.5µg of recombinant GSTO1 (Sigma, GS75-100ug) in PBS in 12uL total volume and incubated in a thermocycler at 37°C for 20 mins in a competitive CMFDA binding assay described previously (28) ...
-
bioRxiv - Biochemistry 2021Quote: ... Recombinant genes were expressed in Escherichia coli strains BL21 (DE3) or TUNER (Novagen), containing the appropriate recombinant plasmid ...
-
bioRxiv - Developmental Biology 2020Quote: ... 0.1 mM β-mercaptoethanol and 1,000 U/ml recombinant leukaemia inhibitory factor (Millipore). For differentiation ESCs were resuspended at 1×104 cells/ml in DMEM (ThermoFisher ...
-
bioRxiv - Immunology 2021Quote: ... and recombinant Wnt3A (Cat no.- 10UG 5036-WN-010 R & D, GF154 Millipore) were purchased from R & D Systems and Millipore ...
-
bioRxiv - Immunology 2022Quote: ... the culture medium was supplemented with 2.5ug/mL recombinant mouse EGF (Sigma-Aldrich) 16 hours before harvesting.
-
bioRxiv - Cell Biology 2022Quote: ... and supplemented with recombinant mouse Leukemia Inhibitory Factor (LIF, 20 U/ml, Millipore). Cultured mESCs at 60% confluency were washed with PBS twice before the preparation of total lysate ...
-
bioRxiv - Developmental Biology 2022Quote: ... FGF treatments were done using recombinant Fibroblast Growth Factor-Basic (F3685, Sigma-Aldrich) concentrations were 300 ng/mL ...
-
bioRxiv - Cell Biology 2022Quote: All experiments were performed using the BDNF mature recombinant (GF029; Merck Millipore, USA) or the C-ter truncated form of BDNF (BDNFΔC-ter) ...
-
bioRxiv - Molecular Biology 2022Quote: Total RNA (10 µg) was incubated with recombinant RNAse-free DNase I (Sigma) in 50 µl-reaction tubes at 37° C for 20 minutes ...
-
bioRxiv - Microbiology 2022Quote: ... RNA samples were DNase I digested (DNase I recombinant, Rnase-free, Millipore Sigma), cleaned up (RNA Clean & Concentrator-5 ...
-
bioRxiv - Biochemistry 2024Quote: ... Recombinant plasmids were transformed into BL21 (DE3) or BL21 (DE3) Tuner cells (Novagen) and were incubated in 1L LB in 2L conical flasks at 37°C ...
-
bioRxiv - Neuroscience 2024Quote: ... Cells were incubated with recombinant Cholera toxin B (CTxB)-FITC (Sigma Aldrich C1655) for 1 h to stain for lipid rafts and then with an anti-TLR4 antibody (Abcam ab22048 ...
-
bioRxiv - Neuroscience 2023Quote: ... Michigan) and 0.6 TIU/mL of recombinant aprotinin was added (Sigma; Burlington, MA). For saliva ...