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Citations for Millipore Sigma :
1701 - 1750 of 10000+ citations for Recombinant Human CTLA4 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... FLAG-tagged proteins (LuxR was tagged N-terminally and H-NS was tagged C-terminally) were captured from clarified lysates using anti-FLAG agarose M2 beads (Sigma). Lysates were incubated with the anti-FLAG resin for 90 minutes at room temperature in immunoprecipitation buffer (50 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: Samples of episomal PPS-GFP Dd2 or endogenously HA-FLAG-tagged PPS Dd2 parasites were harvested by centrifugation and treated with 0.05% saponin (Sigma 84510) in PBS for 5 min at room temperature and spun down by centrifuge at 5,000 rpm for 30 minutes at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were hybridized overnight at 37°C with sixteen FLAP-Y-Cy-3 tagged complementary oligonucleotide probes, targeting regions in Cox7c mRNA (IDT) in Hybridization mix (15 % Formamide, 1.7 % tRNA (Sigma; R1753), 2 % FLAP:Probe mix ...
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Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligationbioRxiv - Microbiology 2020Quote: ... Transduced cell populations were selected with puromycin (2 μg/ml) and expression of flag-tagged NPC1 was confirmed by immunostaining with an anti-Flag M2 antibody (Sigma).
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bioRxiv - Cell Biology 2021Quote: ... U2OS cells plated in six well plates were transfected with 1.8□μg of C-terminally tagged histone H3-HaloTag Fusion Vector DNA and 0.2□μg of C-terminally tagged NSD2-PWWP1-NanoLuc (Wt or F266A) or NSD3-PWW1-NanoLuc using X-tremeGENE HP DNA Transfection Reagent (Sigma), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The peak fractions containing Halo-tagged activating adaptors were collected and concentrated to using a 50K MWCO concentrator (EMD Millipore) to 0.2 mL ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA tagged with 6xHis at the C-terminus was introduced into a pNG2 vector (a derivative of pET-3a, Merck-Novagen) linearized with NdeI and BamHI ...
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bioRxiv - Biochemistry 2022Quote: ... The purification of 3XFLAG-tagged 26S proteasome was carried out by affinity purification using M2 anti-FLAG beads (Sigma A2220). After incubation for 1.5 hrs at 4°C the proteasome was eluted with FLAG peptide ...
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bioRxiv - Genetics 2022Quote: ... Degradation of the AID-tagged RAD21 was induced with addition of 500 μM indole-3-acetic acid (auxin; Sigma-Aldrich). Degradation of AID-tagged WAPL was induced with addition of 1μM 5-Ph-IAA (modified auxin ...
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bioRxiv - Biochemistry 2020Quote: ... The Flag-tagged receptor was detected using mouse anti-Flag M2 primary antibody (1:10,000 dilution; Sigma Aldrich, catalog #F3165) and IRDye® 680RD-conjugated donkey anti-mouse IgG secondary antibody (1:20,000 dilution ...
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bioRxiv - Biochemistry 2020Quote: ... HA-tagged [P2G]CXCL12 was detected using a rabbit anti-HA primary antibody (1:10,000 dilution; Sigma Aldrich, catalog #SAB4300603) and IRDye® 800CW-conjugated goat anti-rabbit IgG (1:20,000 dilution ...
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bioRxiv - Cell Biology 2020Quote: ... After 24 hrs the cells were transiently transfected with Cx26 constructs tagged at the C-terminus with a fluorescent marker (mCherry) according to the GeneJuice Transfection Reagent protocol (Merck Millipore). The HeLa cells stably expressing mouse Cx26 were originally obtained from Dr K ...
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bioRxiv - Molecular Biology 2021Quote: FLAG-tagged RPL3 was transiently expressed in METTL18 KO cells and immunopurified with ANTI-FLAG M2 Affinity Gel (Sigma-Aldrich). The purified proteins were incubated in 1× reaction buffer (50 mM Tris-HCl pH 8.5 and 50 mM MgCl2 ...
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bioRxiv - Genetics 2020Quote: ... The expression of tagged MCM2 in HeLa and Patski cells was confirmed by Western blot using anti-MCM2 rabbit polyclonal antibody (HPA031495 Sigma) at 1:1,000 dilution.
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bioRxiv - Biochemistry 2022Quote: ... Flag-tagged H2B was detected with western blot using mouse anti-FLAG (F3165, Sigma-Aldrich, RRID: AB_259529, 1:3000 diluted) and HRP-conjugated anti-mouse (sc-516102 ...
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: ... RPN11- 3xFLAG tagged subunits were detected by monoclonal anti-FLAG antibodies (Monoclonal ANTI- FLAG® M2 antibody #F3165, Millipore Sigma). The lysate from a wild-type strain with untagged RPN11 was used as a negative control ...
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bioRxiv - Neuroscience 2022Quote: ... HA-tag fluorescence HA-tagged SaCas9 was visualized using the following antibodies: rabbit anti-HA-tag (Sigma-Aldrich, #H6908, RRID:AB_260070) and secondary antibody anti-rabbit Alexa Fluor 488 (1:1000 ThermoFisher ...
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bioRxiv - Biochemistry 2024Quote: ... the cells were transiently transfected with Cx26 constructs tagged at the C-terminus with a fluorescent marker (mCherry) according to the GeneJuice Transfection Reagent protocol (Merck Millipore).
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bioRxiv - Immunology 2024Quote: ... cells expressing GFP-tagged CD80 (Omar S Qureshi et al., 2011) in CHO cell medium (DMEM containing 25 mM D-glucose (Sigma), 1 mM sodium pyruvate (PAA) ...
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... both the excised control spots and the peptide membranes were incubated with HRP-tagged anti-Biotin antibody (1:10,000, Sigma-Aldrich) and washed thrice again with Binding Buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... or full length DDX50) and 50 ng N-terminally tagged HaloTag-GID4 using X-tremeGENE HP DNA Transfection Reagent (Sigma), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... The peak fractions containing Halo-tagged activating adaptors were collected and concentrated to using a 30kDa MWCO concentrator (EMD Millipore) to 0.2mL ...
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bioRxiv - Biochemistry 2021Quote: Lyophilized human haptoglobin phenotype 1-1 (Hp) and human α,β haemoglobin (Hb) were purchased from Sigma Aldrich. Deglycosylation enzyme PNgase F was from New England BioLabs Inc ...
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bioRxiv - Genetics 2020Quote: ... Antibody bound DNA-proteins were isolated by protein G plus/protein A agarose beads (EMD Millipore), washed ...
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bioRxiv - Molecular Biology 2020Quote: ... Sph2-YM4271 cells were grown on synthetic dropout (SD)-His media supplemented with the inhibitor 3 mM 3-amino-1,2,4-triazole (3-AT; Sigma). All Y1H effector plasmids were separately transformed into the Sph2-YM4271 reporter strain and plated on SD/-Leu plates ...
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bioRxiv - Plant Biology 2019Quote: ... The transformants were then spotted on SD (-Trp -Leu -His) selection media containing 0.5/1mM 3-Amino-1,2,4-triazole (3-AT; Sigma, A8056). The positive interactors were then scored based on the stringency of the selection.
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bioRxiv - Plant Biology 2019Quote: ... at least four independent colonies were used for yeast two-hybrid assay on SD-Leu-Trp-His plates with and without 3-amino-1,2,4-triazole (Sigma). Colonies were imaged every 24 h post plating for 4-5 days.
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were then incubated for 4 hours with anti-His hexahistidine antisera conjugated to horseradish peroxidase (HRP) (Sigma) diluted in TBST at a ratio of 1:5000 ...
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bioRxiv - Microbiology 2021Quote: ... Dialysis was performed in concert with cleavage of the His-SUMO tag using the SUMO protease (Sigma Aldrich) at 20U/1mg of protein ...
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bioRxiv - Molecular Biology 2022Quote: CobB driven deacetylation of His-PrsAc was analyzed by Western blot with anti-acetyl lysine antibodies (Sigma/Merck) and mass spectrometry ...
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bioRxiv - Biochemistry 2020Quote: ... 2020) genes fused with an N-terminal His-tag sequence have been inserted into a pET28a vector (Novagen) for protein expression in E ...
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bioRxiv - Cell Biology 2020Quote: Fixed cells were permeabilized using ice-cold Hi-C lysis buffer (10 mM TRIS-HCl pH 8 [Sigma] ...
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bioRxiv - Bioengineering 2021Quote: ... aliquots of each fraction were visualized via immunoblotting (anti-His antibody; Cat: SAB2702218, Sigma Aldrich Canada, Oakville, Canada) to identify fractions enriched for RBD ...
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bioRxiv - Cell Biology 2022Quote: ... HCP-1 was produced similarly but in Hi-5 cells maintained in EX-Cell 405 medium (Sigma-Aldrich) and collected after 66h infection ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was flown through 500 μL of Roche cOmplete™ His-Tag purification resin (Millipore Sigma, #5893682001) for His-tag tagged proteins ...
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bioRxiv - Microbiology 2023Quote: ... The fractions containing His-SSRP1 were concentrated with an Amicon centrifugal filter unit (50 kDa cut-off, Millipore) and buffer exchanged into storage buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Pathology 2023Quote: ... Then 2.4 µL of multiplexed PCR products were mixed with 9.35 µL of Hi-Di formamide (Sigma-Aldrich) and 0.15 µL of Genescan 500 Liz internal line size standard (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... Supernatant was isolated and combined with 2 ml of nickel-nitrilotriacetic acid (Ni-NTA) His Bind resin (Novagen), equilibrated in lysis buffer and incubated for 1 hour at 4°C with gentle rotation ...
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bioRxiv - Synthetic Biology 2023Quote: ... Membranes were then incubated with anti-His primary antibody 1:5000 in 4% milk in PBS-T (Sigma) for 2 hours ...
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bioRxiv - Biochemistry 2024Quote: ... and lysates were clarified by ultracentrifugation (20,000 x g for 20 min) and incubated with 0.4 mL of His-Select nickel resin (Sigma) for 1 h at 4°C with gentle mixing ...
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bioRxiv - Biochemistry 2024Quote: ... and the soluble fraction was purified by affinity chromatography (either Ni2+ or Strep) (His-Select resin (Sigma-Aldrich); Strep-Tactin®XT 4Flow resin (IBA Lifesciences)) ...
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bioRxiv - Biochemistry 2024Quote: Cleaved MntR was loaded onto a 5 ml column of Ni-NTA His•Bind® Resin (Millipore Sigma) equilibrated with Buffer A and then eluted with 40 mM imidazole ...
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bioRxiv - Cell Biology 2020Quote: ... The in vitro interaction experiments using recombinant Ana1 fragments and commercial GST-PBD (Sigma, SRP0360) were carried out as described previously (Alvarez-Rodrigo et al. ...
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bioRxiv - Biophysics 2021Quote: ... Recombinant αE-catenin was expressed via isopropyl 1-thio-β-d-galactopyranoside (IPTG,Sigma-Aldrich) induction ...
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bioRxiv - Developmental Biology 2019Quote: ... 1000U/ml ESGRO® recombinant mice leukemia inhibitory factor (LIF, Millipore, ESG1107, stock 107U/ml) and 2i (1μM PD325 ...
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bioRxiv - Neuroscience 2021Quote: ... the astrocytes were stimulated with TNF recombinant (10 ng /ml) (Sigma-Aldrich Corporation, Missouri, EUA) or MCM (300 µl ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant INO80 complex was eluted with buffer B containing 1.6 mg Flag Peptide (Sigma-Aldrich). Anion exchange chromatography (MonoQ 5/50 GL ...
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bioRxiv - Microbiology 2019Quote: Recombinant L-1-Tosylamide-2-phenylethyl chloromethyl ketone (TPCK)-treated trypsin was purchased from Sigma. The protease inhibitor dec-RVKR-CMK was obtained from Tocris ...
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bioRxiv - Cell Biology 2021Quote: ... coli DH10B cells and cells harboring recombinant plasmids selected on Luria Bertani (LB; Sigma-Aldrich) agar plates with 50 μg/ml kanamycin ...
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bioRxiv - Biochemistry 2020Quote: Recombinant INO80 complex was eluted with buffer B containing 1.6 mg Flag Peptide (Sigma-Aldrich). Anion exchange chromatography (MonoQ 5/50 GL ...