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Citations for Millipore Sigma :
1601 - 1650 of 10000+ citations for Recombinant Human CTLA4 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... and 0.6 µl human serum (Sigma). After two washes with CyFACS ...
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bioRxiv - Cell Biology 2023Quote: ... 1 ug/ml human insulin (Sigma), 10 nM hydrocortisone (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... 20 nM human insulin (Sigma-Aldrich), 25 nM dexamethasone ...
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bioRxiv - Microbiology 2023Quote: ... and human (AB serum, Sigma-Aldrich) for 15 minutes at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... apo-transferrin (apo-transferrin human, Sigma), ferritin (equine spleen ...
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bioRxiv - Cell Biology 2023Quote: ... mouse anti-human Tubulin (T6074, Sigma) at 1:10 000 (WB ...
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bioRxiv - Cell Biology 2023Quote: ... Human bronchial epithelial cells (16HBE14o-, Sigma) were maintained in medium consisting of α-MEM (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... 5% human AB serum (Sigma-Aldrich) and 25 ng/mL IL-2 (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2022Quote: ... 1:2000 human anti-CREST (Sigma) and donkey anti-human Dylight550 ...
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bioRxiv - Molecular Biology 2023Quote: ... Anti-human-CEBPα (Sigma Aldrich, HPA052734) and the secondary antibody (HPR anti-mouse ...
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bioRxiv - Bioengineering 2023Quote: ... rabbit anti-human Oct4 (Millipore, US), and rabbit anti-human HSP90 (Santa Cruz ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 1 % human albumin (Sigma). PBMC were thawed and rested overnight in X-Vivo-20 ...
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bioRxiv - Cell Biology 2024Quote: ... 5% human AB serum (Sigma-Aldrich), 50 μM 2-mercaptoethanol ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-human P2RY12 (Sigma, HPA014518), rabbit anti-human PU.1 (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2024Quote: ... Insulin solution human (Sigma Aldrich, I9278);
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bioRxiv - Biochemistry 2024Quote: ... human Glu-Fibrinopeptide B (Sigma-Aldrich) was recorded throughout the analysis for lock-mass calibration ...
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bioRxiv - Bioengineering 2024Quote: ... thrombin from human plasma (Sigma-Aldrich) and GelMA (8.7% w/v) ...
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bioRxiv - Cell Biology 2024Quote: ... human plasma fibronectin (FC010, Millipore, USA) solution (10 μg/ml in PBS buffer ...
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bioRxiv - Cell Biology 2024Quote: ... Human mammary epithelial cells (Sigma-Aldrich) were cultured with Human Mammary Epithelial Cell Growth Medium (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2024Quote: ... were coated with human IgM (Sigma) at 1:100 for 1 h at 37°C ...
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bioRxiv - Biophysics 2024Quote: ... IgM from human serum (Sigma-Aldrich). We prepared the antibody stock solution in 1X PBS (pH 7.2 ...
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bioRxiv - Immunology 2024Quote: ... purified human C1q (10μg/ml; Millipore) or fresh human serum at 10% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... adding human Apo-transferrin (Sigma, cat.no.T1147), Putrescine dihydrochloride powder ...
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bioRxiv - Cell Biology 2024Quote: ... Proliferating AC16 human cardiomyocytes (Sigma-Aldrich SCC109 ...
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bioRxiv - Molecular Biology 2022Quote: ... and LbaCas12a protein expression for protein purification utilized pET-21a protein expression plasmids (Novagen) (Figure 1).1,25 AspCas12a ...
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Ouabain enhances cell-cell adhesion mediated by β1-subunits of the Na+,K+-ATPase in CHO fibroblasts.bioRxiv - Cell Biology 2019Quote: ... The biotinylated extracts were incubated overnight at 4 °C with 100 μl of streptavidin-agarose suspension (S1638, Sigma). The next day ...
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bioRxiv - Bioengineering 2021Quote: ... Binding of biotinylated S1-RBD to coated VNAR-hFc was detected by streptavidin-HRP (Merck Millipore, 18-152). The signal intensities were collected for each primary VNAR-hFc antibody without competitor and compared to the signal when a competitor antibody was present ...
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bioRxiv - Biochemistry 2019Quote: ... The target of interest is biotinylated either through sortagging or labeled with (+)-Biotin N-hydroxysuccinimide ester (Sigma-Aldrich). For NHS labeling ...
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bioRxiv - Neuroscience 2022Quote: ... Ctip2 or Brn2 incubated sections were reacted with appropriate biotinylated secondary antibody for 1 hour (B7139; Sigma-Aldrich, St ...
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bioRxiv - Cell Biology 2022Quote: We synthesised biotinylated glutathione ethyl ester (bioGEE) by reacting sulfo-NHS-biotin (Thermo) with glutathione ethyl ester (Sigma) in 50mM NaHCO3 pH 8.5 for 2 hours at room temperature ...
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... washed with Binding Buffer and incubated with biotinylated anti-rabbit antibody in Binding Buffer (1:10,000, Sigma-Aldrich). After washing with Binding Buffer ...
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bioRxiv - Neuroscience 2023Quote: ... and a biotinylated donkey anti-rabbit immunoglobulin G secondary antibody (1:1,000 for 2 hours at RT; Millipore). To eliminate nonspecific binding prior to immunostaining endogenous peroxidase activity was blocked using 0.3% H2O2 for 30 min at room temperature (RT) ...
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bioRxiv - Synthetic Biology 2023Quote: We mixed the biotinylated high-affinity (3F10) anti-HA antibodies (50□μg ml−1, ∼500□nM, Sigma-Aldrich) at a 2:1 ratio with Streptavidin (Sigma-Aldrich ...
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bioRxiv - Pathology 2023Quote: ... and then incubated with biotinylated secondary antibody goat anti-rabbit (1:400; Sigma-Aldrich Cat#B8895, RRID: AB_258649), in TBS-Sap for 90 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated with a biotinylated donkey antibody to rabbit IgG (10 μg/ml; AP182B, Merck Millipore) and then with Alexa488-conjugated streptavidin (2.5 μg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... and then incubated with biotinylated secondary antibody goat anti-rabbit (1:400; Sigma-Aldrich Cat#B8895, RRID: AB_258649) in TBS-Sap ...
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bioRxiv - Biochemistry 2020Quote: ... Clarified lysate was loaded onto a gravity column containing 1 mL HIS-Select Nickel Affinity Gel (Sigma) pre-equilibrated with Resuspension Buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... pTB146 His-SUMO-mSTING (residues 139-378) was expressed in Rosetta (DE3) pLysS competent cells (Sigma-Aldrich). Cells were grown in 2×YT medium with 100 μg/mL ampicillin until they reached an OD600 of 1 ...
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bioRxiv - Biochemistry 2019Quote: ... Lysates were then added directly to 96-well His-Select Ni-NTA resin filter plates (Sigma Aldrich) and processed off-robot by centrifugation ...
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bioRxiv - Microbiology 2020Quote: ... and the supernatants were independently applied to a column of HIS-Select Nickel Affinity Gel (Sigma-Aldrich) equilibrated with 50 mM sodium phosphate buffer ...
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bioRxiv - Biophysics 2021Quote: ... followed by cleavage of the His-tag with 3C protease and dephosphorylation with bovine alkaline phosphatase (Sigma). Further purification was performed by ion exchange chromatography (Source Q for WT and the S897E ...
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bioRxiv - Biophysics 2020Quote: ... and passed through pre-equilibrated HIS-Select Ni-nitrilotriacetic acid resin (Ni-NTA) (Sigma-Aldrich Co., USA) at 4 °C for binding of 6x-His tagged protein ...
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bioRxiv - Cell Biology 2022Quote: ... The following primary antibodies were used: anti-His (1: 2000, Cat No: 70796-4, Novagen, Madison, WI); anti-DNALI1 (1 ...
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bioRxiv - Cell Biology 2019Quote: ... The His-tag was removed by cleavage with 16 U of thrombin (Ref 27-0846-01, Sigma) added directly onto the beads for 2h at 25°C ...
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bioRxiv - Biochemistry 2021Quote: ... pneumoniae LicB with an N-terminal 10×His affinity tag in a modified pET-19b vector (Novagen) was overexpressed in E ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... plasmid coding for 6x-His-Cas9 were transformed in Rosetta DE3 Novagen competent cells (Merck Millipore, USA). Protein production was induced using autoinduction medium (Formedium ...
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bioRxiv - Biophysics 2021Quote: ... The His-eluent was concentrated using a 30 kDa cutoff Amicon Ultra-4 centrifugal filter (Millipore UFC803008), filtered using a 0.22 μm Millex-GP PES membrane (Millipore SLGP033RS) ...
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bioRxiv - Microbiology 2022Quote: ... with the following modifications: Mouse anti-GST tag or mouse anti-His tag 1:200× (Sigma-Aldrich) and HRP-conjugated goat anti-mouse IgG 1:2,000× (Seracare Life Sciences ...
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bioRxiv - Genetics 2023Quote: ... imidazole was removed from the eluted His-SIRT5 using ultra-0.5 centrifugal filter units (UFC501096, Merck Millipore). 500 µl of the recombinant SIRT5 elution fractionation was added to the ultra-0.5 centrifugal filter units and centrifuged at 14,000 x g for 15 min at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... pTB146 His-SUMO-mSTING (residues 139-378) was expressed in Rosetta (DE3) pLysS competent cells (Sigma-Aldrich). Cells were grown in 2xYT medium with 100 μg/mL ampicillin until they reached an OD600 of 1 ...