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Citations for Millipore Sigma :
1451 - 1500 of 10000+ citations for 6 Chloro 7H pyrrolo 2 3 d pyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: The mGluR5 specific negative allosteric modulator CTEP (chloro-4-((2,5-dimethyl-1-(4-(trifluoromethoxy)phenyl)-1H-imidazol-4-yl)ethynyl)pyridine) was purchased from Sigma-Aldrich (SML2306) and administered at 2 mg/kg concentration by intraperitoneal (i.p. ...
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bioRxiv - Cell Biology 2021Quote: ... XL-413) for 1 h followed by inhibitor incubation together with 100 μM 5-chloro-2ʹ-deoxyuridine (CldU; Sigma-Aldrich, Germany) and ...
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bioRxiv - Microbiology 2022Quote: ... the samples were permeabilized with TritonX-100 at 0.1% in D-PBS with subsequent blocking with 3% Bovine Serum Albumin (Sigma-Aldrich) in D-PBS ...
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bioRxiv - Genomics 2020Quote: BJ cells (Fig. 3) and A549 cells (Fig. S10) were treated with 5 μg/mL of Actinomycin D (Sigma, A9415), which inhibits transcription by halting elongation of the transcribed RNA (42 ...
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bioRxiv - Biochemistry 2020Quote: ... Bound protein was eluted seven times with 0.5 column volumes of buffer A with 3 mM D-Desthiobiotin (Sigma-Aldrich), 0.026% DDM and 0.1 mM AEBSF protease inhibitor ...
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bioRxiv - Biochemistry 2022Quote: ... The column was washed with 30 column volumes (CV) of wash buffer before bound proteins were eluted with wash buffer supplemented with 3 mM D-desthiobiotin (Sigma). The protein was transferred into wash buffer without D-desthiobiotin and incubated overnight at 4°C with 1:100 (w/w ...
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bioRxiv - Molecular Biology 2022Quote: ... were diluted in PBS together with 3 μg of D-Val-Leu-Lys 4-nitroanilide dihydrochloride chromogenic substrate (S-2251) (Sigma) and 1 μg of FhNEJ-Teg or recombinant protein in the presence or absence of 15 ng t-PA (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... 0.01% C12E9) and elution was performed using 3 column volumes of buffer C supplemented with 1 mg/mL d-desthiobiotin (Sigma). The elution fraction was concentrated to 0.5 mg/mL using 100-kDa cut-off filters (Millipore ...
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bioRxiv - Immunology 2023Quote: ... ex vivo isolated naïve or overnight stimulated (anti-CD40 at 5μg/ml and IL-4 at 1ng/ml) 3×105 B cells were plated on a poly-D lysine (PDL) (Sigma)-coated XF96 cell culture microplate and incubated at 37 °C for a minimum of 30 min in a CO2-free incubator in assay medium (XF RPMI medium pH 7.40 supplemented with 2 mM L-glutamine ...
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bioRxiv - Pathology 2023Quote: Mouse epidydimal adipose tissue was harvested and digested in Hanks’ Balanced Salt Solution (HBSS, no calcium and magnesium) containing fetal bovine serum (FBS,3%) and collagenase D (2.5 mg/ml, Sigma-Aldrich) for 30 minutes at 37°C under agitation ...
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bioRxiv - Immunology 2024Quote: ... approximately 30 g of spleen tissue was placed in R10 supplemented with 10U/mL DNase I and 3 mg/ml collagenase D (Sigma), the capsule was removed ...
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bioRxiv - Immunology 2019Quote: ... the stock solution of 6-hydroxydopamine (6-OHDA) (Sigma) was prepared in water at 42 mg/mL and stored at −20°C ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with interleukin-6 (IL-6, Sigma) for 24 ...
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bioRxiv - Neuroscience 2023Quote: ... 6-OHDA (10mg/ml; 6-OHDA hydrobromide, Sigma-Aldrich) was dissolved in a vehicle solution immediately before the surgeries ...
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bioRxiv - Biophysics 2021Quote: ... D-(+)-Glucose (D-Glu; Sigma-Aldrich, 20 mM) or L-(–)-Glucose (L-Glu ...
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bioRxiv - Systems Biology 2021Quote: ... plus D-glucose (EMD) and D-galactose (Sigma). Cultures were grown in a humidified incubator (Infors Multitron ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... D-mannitol and D-trehalose dihydrate (Sigma-Aldrich) at a concentration between 50 and 500 mg/mL were prepared by simple mixing (Table 1) ...
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bioRxiv - Cancer Biology 2019Quote: Tumors were harvested and mechanically dissociated for 30 min with collagenase D (2 mg/ml; Sigma-Aldrich) and DNase I (4 U/ml ...
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bioRxiv - Molecular Biology 2020Quote: Femur preparation was done on ice with PBS supplemented with 2 μg/mL Actinomycin D (Sigma Aldrich). Segments were digested in 10 mg/ml Collagenase II (Nordmark ...
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bioRxiv - Genomics 2019Quote: We added 2 ml of ice-cold Buffer D (10 ml of Sucrose Cushion solution (Sigma-Aldrich), 1.2 ml of Sucrose Cushion buffer (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2019Quote: ... GluR inhibition was performed using 40 µM D(-)-2-amino-5-phosphonopentanoic acid (APV, #A8054, Sigma Aldrich) and 2 µM 6,7-dinitroquinoxaline-2,3-dione (DNQX ...
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bioRxiv - Cell Biology 2020Quote: Corneas were extracted from mice after euthanasia and treated with 2 mg/mL collagenase D (Sigma C0130) for 1 h at 37°C and triturated with a pipet tip ...
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bioRxiv - Microbiology 2021Quote: ... 50 μM Antimycin A and 1% DMSO and d) 100 mM 2-DG (Sigma-Aldrich: D8375-1G). Each condition was tested in triplicate.
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bioRxiv - Cell Biology 2021Quote: ... The parasites (ring stage; 2-4 hpi) were tightly synchronized with 5% (v/v) D-sorbitol (Sigma) and then monitored by Giemsa staining of methanol-fixed blood smears ...
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bioRxiv - Cell Biology 2022Quote: ... carefully washed five times and resuspended in SD-N medium (2% (w/v) α-D-glucose (Sigma), 0.17% (w/v ...
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bioRxiv - Cell Biology 2023Quote: Yeast cells were grown in synthetic defined complete (SDC) containing 2% (w/v) α-D-glucose (Sigma), 0.7% (w/v ...
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bioRxiv - Biochemistry 2023Quote: ... The medium was supplemented with 2 µM of the antimitotic agent (1-β-D-Arabinofuranosylcytosine (Sigma Aldrich)) and 100 ng/ml NGF (Alomone labs) ...
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bioRxiv - Cell Biology 2023Quote: The following chemicals and reagents were used in this study: 2-Deoxy-D-glucose (D8375-1G, Sigma), 2-mercaptoethanol (BME ...
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bioRxiv - Biochemistry 2023Quote: ... pH 6.5-6.6) was prepared according to the manufacturer’s recipe and supplemented with either 2%D-glucose (Sigma), 2% maltose monohydrate (VWR) ...
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bioRxiv - Bioengineering 2023Quote: ... MIK665 treatments were delivered in 2% vitamin E/d-α-tocopheryl polyethylene glycol 1000 succinate (Sigma-Aldrich) in NaCl 0.9% (wt/vol ...
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bioRxiv - Molecular Biology 2023Quote: ... Total DNA was labeled with 2 μg/ml of 7-AAD (7-amino-actinomycin D, Sigma-Aldrich) diluted in PBS containing 100 μg/ml of RNAse A (Sigma -Aldrich) ...
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bioRxiv - Biochemistry 2022Quote: ... pLANT-2/RIL–RFC[1+5] was co-transformed with pET(11a)-RFC[2+3+4] into BLR(DE3) cells (Novagen, Madison, Wisconsin). The cell lysate was clarified by centrifugation ...
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bioRxiv - Microbiology 2021Quote: ... Three subsamples (∼2 ml) were taken from each section with a 3 ml syringe and immediately fixed in 2 ml RNAlater (Sigma Life Science) in a 5 ml tube (Qiagen ...
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bioRxiv - Immunology 2022Quote: Fabs (2-4 mg/ml) were mixed with a 2 to 3-fold molar excess of fen-G4 or fentanyl (Sigma F-013) in Dulbecco’s PBS and incubated for 1-2 hours at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: A stock solution (74.8 mM) of the MEK inhibitor 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one (PD98059, PD hereafter, Sigma-Aldrich, Argentina) was conserved at −20 °C and freshly diluted in DMSO to the desired concentration (11.22 mM ...
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bioRxiv - Biochemistry 2022Quote: ... were mixed with a stock solution of 40% positively charged (3-acrylamidopropyl)-trimethylammonium chloride (75% wt, Aldrich) or negatively charged 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS, Sigma-Aldrich, Inc) containing N,N’-methylenebisacrylamide (Sigma ...
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bioRxiv - Neuroscience 2022Quote: One day before plating, 6-well MEA plates (Axion Biosystems, M384-tMEA-6W) were coated with poly-D-lysine (Millipore Sigma P6407-5MG) in borate buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... and Glucose-6-phosphate dehydrogenase (G-6-PDH) with anti-G-6-PDH antibody (Sigma A9521). Blots were probed with IgG Goat anti-Rabbit (BioRad 170-6515 ...
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bioRxiv - Immunology 2021Quote: The livers of freshly-sacrificed mice were perfused retrogradely via the IVC(3) with 3 ml of PBS and then 10 ml of 2% paraformaldehyde (Sigma, catalogue# 30525-89-4) in PBS ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: When the tumor sizes reached to 80 mm3 animals were treated with a PFKFB3 inhibitor 3-(3-pyridinyl)-1-(4-pyridinyl)-2-propen-1-one) (3PO) (Sigma-Aldrich, Merck, Overijse, Belgium). The animals received intraperitoneal (i.p. ...
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bioRxiv - Cancer Biology 2021Quote: ... 20 μl of 5mg/ml MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (Sigma) was added to each well ...
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bioRxiv - Cell Biology 2020Quote: Spheroids were cultured on dishes coated with 3% poly-2-hydroxyethyl methacrylate (polyHEMA) (Sigma, P3932), which was dissolved in 95% absolute ethanol overnight in magnetic stirrer at room temperature (RT) ...
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bioRxiv - Cell Biology 2020Quote: ... RPE1 cells and LCLs were treated with 3 mM or 2 mM thymidine (Sigma, T9250) for 22 hours ...
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bioRxiv - Cell Biology 2022Quote: ... Media was changed every 2-3 days for new media: DMEM/F-12 (Sigma-Aldrich) + 10% FBS (Gibco ...
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bioRxiv - Microbiology 2019Quote: The mitochondria-dependent reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma) to formazan was used to assess cell viability ...
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bioRxiv - Cancer Biology 2019Quote: Cell viability was accessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma) assay ...
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bioRxiv - Cancer Biology 2019Quote: Cell viability was accessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma) assay ...
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bioRxiv - Genomics 2020Quote: ... 3·106 cells were fixed in 400 μl of DPBS/2% Paraformaldehyde (Sigma P-6148) for 10 minutes at room temperature shaking ...
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bioRxiv - Neuroscience 2021Quote: ... [3H]3-(2-carboxypiperazin-4-yl) propyl-1-phosphonic acid (CPP, 10 μM, Sigma-Aldrich) and a selective group III metabotropic glutamate receptor agonist ...
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bioRxiv - Neuroscience 2021Quote: ... [3H]3-(2-carboxypiperazin-4-yl) propyl-1-phosphonic acid (CPP, 10 μM, Sigma-Aldrich) and a selective group III metabotropic glutamate receptor agonist ...