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Citations for Millipore Sigma :
1301 - 1350 of 10000+ citations for 6 Chloro 7H pyrrolo 2 3 d pyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... 6-Formylindolo[3,2-b]carbazole (FICZ) and 2-Methyl-2H-pyrazole-3-carboxylic acid (2-methyl-4-o-tolylazo-phenyl)-amide (CH-223191) from Sigma-Aldrich. 1-HP ...
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bioRxiv - Neuroscience 2020Quote: ... were performed in DJ-1-deficient cells with MTT (3-(4, 5-dimethylthiazol-2-yl)−2–5-diphenyltetrazolium bromide) (Sigma-Aldrich) as described in [24] ...
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bioRxiv - Biochemistry 2022Quote: ... they were cultivated 72 hours in the presence of 2 μM DL-threo-1-phenyl-2-palmitoylamino-3-morpholino-1-propanol (PPMP) (Sigma-Aldrich) to inhibit the synthesis of glucosylceramide-based GSLs 75 and incubated with fluorescent SaroL-1 as previously described ...
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bioRxiv - Developmental Biology 2019Quote: ... 2 mg of protein lysate were incubated for 3 h with 2 μg of α-FLAG M2® antibody (Sigma-Aldrich) pre-coupled to 20 μl of rotein G Dynabeads® (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... Mice were subjected to cutaneous immune challenge by applying 20 µl of 3-5% 4- Ethoxymethylene-2-phenyl-2-oxazolin-5-one (Sigma-Aldrich) in 1:2 acetone:olive oil ...
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bioRxiv - Biochemistry 2019Quote: apoTaLPMO9A was prepared by dialyzing the purified TaLPMO9A extensively using D-tube Dialyzer Maxi (6-8 kDa) from Millipore (Billerica, MA, USA). Dialysis was performed in 20 mM phosphate buffer (pH 7 ...
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bioRxiv - Genetics 2021Quote: ... Mouse neural progenitor cells were routinely passaged 1:2-1:4 every 3-5 days using Accutase and maintained in NS expansion medium on laminin-coated plates (Sigma, 3 μg/ml). Both mESCs and mNPCs were incubated at 37 °C and 5% CO2 ...
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bioRxiv - Genetics 2020Quote: ... containing either tunicamycin only (Enzo Life-Sciences; 2-3 μg/mL) or tunicamycin and auxin (IAA, indole-3-acetic acid, Sigma-Aldrich; 1 mM), and seeded with OP50-1 bacteria ...
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bioRxiv - Immunology 2021Quote: Blood was extracted from hBLT mice at 3 and 5-6 weeks post-infection and lysed with Red Blood Cell Lysis Buffer (SIGMA). T cells were activated for 1.5 h with 5μl/ml of anti-CD28 and anti-CD49d in the presence or absence of 6.4μg/ml of a Gag pool of peptides in the presence of 0.5μg/ml Brefeldin A ...
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bioRxiv - Bioengineering 2019Quote: Cy3-(GT)6-SWCNTs and Cy3-(GT)30-SWCNTs were first filtered 3 times using 100kDa Amicon centrifuge filters (Millipore) to remove free Cy3-DNA from solution ...
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bioRxiv - Bioengineering 2019Quote: ... Laccase activity was followed by the oxidation of 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) (Sigma-Aldrich, USA) (33) ...
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bioRxiv - Biochemistry 2019Quote: ... 3 mM KCl) and harvested in a PBS lysis buffer (PBS plus 6 uL/mL protease inhibitors cocktail (Sigma P8340), 0.6 mM PMSF ...
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bioRxiv - Cell Biology 2022Quote: ... and 3 mL volumes of culture were transferred per well to 6-well tissue culture-treated polystyrene plates (Sigma CLS3506) and incubated at 23°C for 48 hours ...
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bioRxiv - Cell Biology 2022Quote: ... Liposomes consisting of DPPC/egg yolk PC/egg yolk PG/cholesterol (6:3:1.2:0.95, DPPC from Avanti Polar Lipids, all other Sigma) with 0.6 μCi tracer [2-palmitoyl-1-14C]-dipalmitoyl phosphatidylcholine (14C-DPPC ...
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bioRxiv - Microbiology 2022Quote: ... Samples were centrifuged 1800 rpm for 6 minutes at 4°C and pellets were resuspended n 3 mL RBC lysis for 4 minutes (Sigma). Samples were centrifuged 1800 rpm for 6 minutes at 4°C and the pellets were resuspended in 200 μl of PBS + 2% FBS + 2 mM EDTA ...
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bioRxiv - Microbiology 2020Quote: ... and the resulting adhered bacteria on the cover slip were stained with 200 μL of the membrane stain N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide (FM4-64) (Sigma) at a final concentration of 20 μg/mL for 5 minutes ...
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bioRxiv - Neuroscience 2019Quote: hNSG and non-engrafted NSG mice (4-6 months old) were injected 3 mg/kg (i.p.) with lipopolysaccharide (LPS from E. coli; O55:B5, Sigma-Aldrich) or 0.9% saline solution ...
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bioRxiv - Immunology 2020Quote: ... Cells were then treated with 6-thio-dG (3-5 μM) in the presence of 500 ng/ml doxycycline (Sigma), washed three times with warm PBS and allowed to recover in regular growth medium containing 500 ng/ml doxycycline ...
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bioRxiv - Molecular Biology 2021Quote: Total splenocytes were isolated from C57BL/6 and mFICD-/- mouse spleens and then treated for 3 days with 20 μg/ml LPS (Sigma), 100 µg/ml heparan sulfate or 2.5 μM thapsigargin (Enzo Life Sciences) ...
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bioRxiv - Cancer Biology 2022Quote: ... Lentiviral transductions were performed in a 6-well plate format (3 × 105 cells/well) using 10 µg/mL polybrene (Merck Millipore). Stably transduced cells were flow-sorted.
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bioRxiv - Cell Biology 2022Quote: ... The following incubations ensued at room temperature: 3×10 min in 0.1 M maleic acid buffer (Sigma-Aldrich; pH 6), 2 h in the dark in 0.5% (w/v ...
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bioRxiv - Neuroscience 2022Quote: ... using 0.5 mM EDTA and either re-seeded with split ratios (1:3 to 1:8) onto 6-well plates or cryopreserved using CryoStor CS10 Cryopreservation Reagent (Sigma). iPSC lines were maintained in 5% CO2 ...
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bioRxiv - Neuroscience 2022Quote: ... mito-tempol[23] (25 μg, Sanbio) and oligodeoxynucleotide (3 μg/μl day 4, 5 and 6 after carrageenan, Sigma-Aldrich), were performed under light isoflurane anesthesia as described.[8a ...
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bioRxiv - Immunology 2022Quote: ... Aliquots of 3 µl of between 3-6 mg/ml of Toll5A-Spz1C gel-filtered complex mixed with 8 mM CHAPSO (final concentration, Sigma) were applied to the grids ...
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Transport of metformin metabolites by guanidinium exporters of the Small Multidrug Resistance familybioRxiv - Biophysics 2023Quote: ... 100 mM KCl and pre-loaded with 0.4 mM substrate (Gdm+ or guanylurea) and 1mM pyranine (trisodium 8-hydroxypyrene-1, 3, 6-trisulfonate; Sigma-Aldrich) using three freeze/thaw cycles ...
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bioRxiv - Cell Biology 2023Quote: ... 3 – 6 mg of cell extract and 20 μl of α-Flag M2 affinity bead suspension (Sigma, catalog no.: A2220) were used ...
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bioRxiv - Cancer Biology 2023Quote: ... Organoids were passaged at a 1:3 to 1:6 ratio every 7 days using cell dissociation solution-non enzymatic (Sigma) and plated in fresh BME matrix droplets.
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bioRxiv - Cell Biology 2020Quote: ... embryos/egg chambers were stained using 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich, St Louis, MO). Lipid droplets were stained using either Nile Red (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... Slides were incubated in 4’,6-diamidino-2-phenylindole (DAPI) to label nuclei (1:10,000 dilution, Sigma). TUNEL assay was conducted with ApopTag Fluorescin Direct In Situ Apoptosis Detection Kit (Millipore ...
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bioRxiv - Developmental Biology 2021Quote: ... the leaves were harvested and treated with 0.5 mg/ml DAPI (4ʹ,6-diamidino-2-phenylindole; Sigma). A Zeiss LSM 510 confocal scanning microscope was used to collect images.
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bioRxiv - Neuroscience 2021Quote: ... brain slices were also stained by incubating with 4’,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich, USA) to mark all cell nuclei for 2 days ...
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bioRxiv - Neuroscience 2021Quote: ... brain slices were also stained by incubating with 4’,6-diamidino-2-phenylindole (DAPI, Sigma-Aldrich, USA) to mark all cell nuclei for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was isolated from 2-week-old seedlings or 6-week-old plants using Trizol (Sigma) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Stained cells were washed three times with PBS containing 4’,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich). Image were taken with a FLUOVIEW FV1000 ...
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bioRxiv - Neuroscience 2020Quote: ... Cell nuclei were stained with 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI, 1 µg/ml, Sigma-Aldrich). Sections were coverslipped using Vectashield Antifade Mounting Medium (Vector Labs ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were counterstained with DAPI (4′,6-diamidino-2-phenylindole dihydrochloride; 5 µg/ml, Sigma-Aldrich) at room temperature for 20 minutes.
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bioRxiv - Biochemistry 2020Quote: ... for 20 min and optionally counterstained with 1 μg/ml DAPI (4’,6-diamidino-2-phenylindole, Sigma) nucleic acid stain ...
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bioRxiv - Cell Biology 2021Quote: ... The biotinylation reaction was quenched by 3 washes of a stop solution prepared with 10 mM 6-Hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox; Sigma), 20 mM sodium Ascorbate and 10 μM sodium azide in PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... the eIF4EWT and eIF4EKI mice (6-8 weeks old) were treated with 2 μl 4-HT (Sigma) at 5 mM working concentration for 3 continuous days ...
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bioRxiv - Neuroscience 2020Quote: ... and counter-stained using 4’,6-diamidino-2-phenylindole (DAPI) for nuclei (Sigma, D9542-10MG, 1:1000). Confocal images of the cortex of non-transgenic littermates and arcAβ mice were obtained using a Leica SP8 confocal microscope (Leica Microsystems GmbH ...
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bioRxiv - Microbiology 2021Quote: ... samples were treated with 10 μg/mL of 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI, Sigma-Aldrich) in PBS ...
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bioRxiv - Microbiology 2020Quote: ... Nuclear DNA was stained with DAPI (4’,6’-diamidino-2-phenylindole) (0.1 µg/ml; Sigma-Aldrich, Poland). Immunostained cultures were mounted on glass slides in ProLong Gold antifade medium (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Slides were inserted into a 1 μg/ml solution of 4’,6-diamidino-2-phenylindole (DAPI, Sigma) for 10s and ...
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bioRxiv - Cell Biology 2022Quote: ... nuclei were counterstained with 500 ng/mL of 4′,6-diamidino-2-phenylindole (DAPI, Sigma, CAT#D8417) for 10 minutes at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... sections were stained with DAPI (4’,6-diamidino-2-phenylindole; Sigma-Aldrich Corp., St. Louis, MO, USA) and cover slipped with Fluoromont-G ...
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bioRxiv - Biophysics 2020Quote: Unsized unilamellar DOPG liposomes containing Laurdan (6-Dodecanoyl-N, N-dimethyl-2-naphthylamine, from Sigma, Taufkirchen, Germany) (molar ratio lipid:Laurdan=1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were washed 1x and incubated with 0.2µg/mL DAPI (4’,6-diamidino-2-phenylindole, Sigma-Aldrich) for 10min at RT ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were mounted after 4′,6-diamidino-2-phenylindole (DAPI) staining (1:5 000, Sigma-Aldrich, USA). Confocal images were captured under 20× objective using A-1R Confocal Microscope (Nikon ...
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bioRxiv - Developmental Biology 2022Quote: ... and (+−)-6-hydroxy-2,5,7,8- tetra- methylchromane-2-carboxylic acid (Trolox) (cat: 238813-5 G) were ordered from Sigma. Potassium chloride (cat ...
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bioRxiv - Immunology 2019Quote: ... Then cells were stained with a 1/15000 dilution of 4’,6-diamidino-2-phenylindole (DAPI) (Sigma) and mounted in antifading agent Citifluor AF1 (Citifluor Ltd.) ...